##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552202_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	339783
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.680463707719337	30.0	25.0	33.0	16.0	33.0
2	28.602902440675372	31.0	28.0	33.0	16.0	34.0
3	28.400143621075806	31.0	27.0	33.0	16.0	34.0
4	32.44988124773753	35.0	32.0	35.0	28.0	37.0
5	33.73374182934402	35.0	33.0	35.0	30.0	37.0
6	33.34365756968418	35.0	33.0	36.0	28.0	37.0
7	34.3937071601581	35.0	35.0	37.0	32.0	37.0
8	34.49715848055965	35.0	35.0	37.0	32.0	37.0
9	36.395431790289685	39.0	35.0	39.0	31.0	39.0
10	36.15784780286241	37.0	35.0	39.0	32.0	39.0
11	36.254282880544345	37.0	35.0	39.0	32.0	39.0
12	36.14755594011472	37.0	35.0	39.0	32.0	39.0
13	36.32398619118673	38.0	35.0	39.0	32.0	39.0
14	37.29972364715127	39.0	36.0	40.0	32.0	41.0
15	37.544035457924615	39.0	36.0	41.0	32.0	41.0
16	37.35304002848877	39.0	36.0	41.0	32.0	41.0
17	36.40717457906958	38.0	34.0	40.0	30.0	41.0
18	36.12258411986474	38.0	34.0	39.0	30.0	41.0
19	35.58681275990853	37.0	34.0	39.0	29.0	41.0
20	35.96594002642863	37.0	35.0	39.0	31.0	41.0
21	36.34694201887675	38.0	35.0	40.0	32.0	41.0
22	36.75061436269619	38.0	35.0	40.0	32.0	41.0
23	36.982276923801365	38.0	35.0	40.0	33.0	41.0
24	36.90181969080266	38.0	35.0	40.0	33.0	41.0
25	36.154348510667106	38.0	35.0	40.0	31.0	41.0
26	36.378170773699686	38.0	35.0	40.0	31.0	41.0
27	36.718976523251605	38.0	35.0	40.0	33.0	41.0
28	36.64395805558253	38.0	35.0	40.0	32.0	41.0
29	36.699007896216116	38.0	35.0	40.0	32.0	41.0
30	36.30131583981541	38.0	35.0	40.0	31.0	41.0
31	36.100405258650376	38.0	35.0	40.0	31.0	41.0
32	35.82818445890465	38.0	35.0	40.0	30.0	41.0
33	35.443259374365404	38.0	35.0	40.0	25.0	41.0
34	35.096929510893716	39.0	35.0	40.0	22.0	41.0
35	34.83078023326652	39.0	35.0	40.0	20.0	41.0
36	34.557326293546176	38.0	35.0	40.0	18.0	41.0
37	34.507247272523934	38.0	35.0	40.0	18.0	41.0
38	34.30362025174891	38.0	34.0	40.0	18.0	41.0
39	34.207276408766774	38.0	34.0	40.0	17.0	41.0
40	34.05345470491461	38.0	34.0	40.0	16.0	41.0
41	33.84371201619857	38.0	34.0	40.0	15.0	41.0
42	33.853724288737226	38.0	34.0	40.0	15.0	41.0
43	33.77986832772682	38.0	34.0	40.0	15.0	41.0
44	33.64406989166615	38.0	33.0	40.0	15.0	41.0
45	33.57071424997719	38.0	33.0	40.0	15.0	41.0
46	33.45056992256823	37.0	33.0	40.0	15.0	41.0
47	33.44987830468269	37.0	33.0	40.0	15.0	41.0
48	33.37244064594167	37.0	33.0	40.0	15.0	41.0
49	33.34128546748954	37.0	33.0	40.0	13.0	41.0
50	33.24412934137376	37.0	33.0	40.0	13.0	41.0
51	31.474956074906633	35.0	30.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	13.0
10	27.0
11	28.0
12	19.0
13	16.0
14	19.0
15	40.0
16	75.0
17	162.0
18	318.0
19	477.0
20	798.0
21	1172.0
22	1711.0
23	2600.0
24	3982.0
25	6320.0
26	8861.0
27	9930.0
28	9158.0
29	8626.0
30	8520.0
31	9840.0
32	12759.0
33	18520.0
34	25132.0
35	31172.0
36	39530.0
37	59561.0
38	63261.0
39	17132.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.13390016569399	16.275093221261805	19.486554653999757	23.10445195904445
2	41.823752218327584	22.867536045064053	20.82505599161818	14.483655744990184
3	27.92664730136587	22.40665365836431	35.30694590370913	14.359753136560688
4	24.294917638610524	21.298593514095764	38.391267367702326	16.015221479591386
5	23.888187460820582	24.582748401185462	34.399602098986705	17.129462039007247
6	21.195880900457055	31.477148650756515	35.18098315689719	12.145987291889234
7	65.08742344378618	5.741899977338478	25.28407836766407	3.8865982112112727
8	64.73013658717475	7.44445719768205	23.060894747530043	4.764511467613152
9	59.56890132820064	6.708987795151611	24.45707995985673	9.26503091679101
10	32.3609480168225	22.208880373650242	31.154295535680127	14.275876073847133
11	27.010180026664077	21.56552858736311	35.41995920926003	16.004332176712786
12	23.25660789386167	20.1413843541319	40.09470750449552	16.507300247510912
13	20.763251840145035	21.30124226344461	42.923571809066374	15.011934087343981
14	19.145454598964633	24.687521153206603	38.90365321396303	17.26337103386573
15	17.327529629204523	21.52991762389525	45.351297740028194	15.791255006872031
16	19.194309309176738	21.903391282082975	39.9375483764638	18.96475103227648
17	19.256113460649885	20.31002139600863	40.07204598228869	20.36181916105279
18	20.914819163995844	20.23026461005995	40.48319074232672	18.371725483617485
19	20.34828110882534	22.273921885438646	38.18760797332415	19.190189032411865
20	21.778900062687068	21.915457806894402	40.04232112848494	16.263321001933587
21	20.531044813895928	24.53948549515426	38.40951430766106	16.519955383288746
22	19.52805172713173	20.24762863356907	38.5192902528967	21.705029386402497
23	18.512992115556102	24.047701032717942	38.875988498541716	18.56331835318424
24	19.545415750640853	20.32620819758493	39.27300659538588	20.855369456388342
25	17.907605736602477	24.321405132099017	38.52841372287607	19.242575408422436
26	18.40380478128688	21.568471642195167	39.40397253541231	20.62375104110565
27	19.23198041102704	22.403121992565843	38.31209919272006	20.05279840368706
28	16.44902776183623	22.10911081484359	42.04654146911411	19.395319954206066
29	19.89534497017214	21.347742529791073	38.733544644670275	20.023367855366516
30	18.05917306045329	22.156788303122877	39.41486183829091	20.369176798132926
31	20.609035766945375	21.643225234929353	38.223218936792016	19.524520061333263
32	21.49106930011213	22.788662175564994	37.272317920555174	18.447950603767698
33	18.5235871129515	22.388995329371983	35.66334984387094	23.424067713805577
34	20.372414158448183	22.479935723682466	37.7370262785366	19.41062383933275
35	19.974218839671202	22.852820770903783	34.38930140707451	22.7836589823505
36	20.868907508615795	24.11185962805673	35.73310024339064	19.28613261993684
37	19.57984949217589	24.12863504059944	36.38528119417393	19.906234273050742
38	19.070112395264037	25.125447712216324	34.128252443471276	21.676187449048363
39	20.6534758949094	22.403710603532254	35.961186992874865	20.981626508683483
40	22.047306663370446	22.55939820414794	34.404899597684405	20.988395534797206
41	19.314680251807772	23.75633860434454	35.55916570281621	21.36981544103148
42	21.784197561384765	22.578822366039503	35.28487299246873	20.35210708010701
43	21.453692503745035	22.14413316734504	34.54763775703905	21.85453657187087
44	20.310904312458245	23.2418926197014	34.581777193090886	21.865425874749473
45	20.164045876338722	22.50907196651981	34.028482884664626	23.298399272476846
46	21.742111877286384	23.60830294629219	33.56083147185115	21.08875370457027
47	18.680157630016804	22.957593522924927	37.975708025416225	20.38654082164205
48	20.360347633636763	22.990261431560732	34.820753245453716	21.82863768934879
49	19.757021393065575	21.27740351930497	37.478331758799	21.48724332883046
50	19.218442358799585	22.04877819078647	36.231948037423884	22.500831412990056
51	19.198429585941614	21.843352963509062	33.59997410111748	25.35824334943184
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	324.0
1	681.0
2	1038.0
3	9029.0
4	17020.0
5	11147.0
6	5274.0
7	5111.5
8	4949.0
9	4783.0
10	4617.0
11	4615.5
12	4614.0
13	4297.0
14	3980.0
15	3756.0
16	3532.0
17	3228.0
18	2924.0
19	2749.5
20	2575.0
21	2526.0
22	2477.0
23	2559.5
24	2642.0
25	2820.5
26	3069.5
27	3140.0
28	3343.0
29	3546.0
30	4059.5
31	4573.0
32	5081.5
33	5590.0
34	6403.5
35	7217.0
36	7752.0
37	8287.0
38	9265.5
39	10244.0
40	11500.0
41	12756.0
42	14366.5
43	15977.0
44	17773.5
45	19570.0
46	27847.0
47	36124.0
48	31432.5
49	26741.0
50	26652.5
51	26564.0
52	23766.0
53	20968.0
54	18981.0
55	16994.0
56	15852.5
57	14711.0
58	13583.0
59	12455.0
60	11825.5
61	11196.0
62	9861.5
63	8527.0
64	7149.5
65	5772.0
66	4807.0
67	3842.0
68	3190.0
69	2538.0
70	2134.5
71	1731.0
72	1288.0
73	845.0
74	652.0
75	334.0
76	209.0
77	168.0
78	127.0
79	89.0
80	51.0
81	40.5
82	30.0
83	20.5
84	11.0
85	10.5
86	10.0
87	8.5
88	7.0
89	4.0
90	1.0
91	2.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	339783.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.97947751339315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.89808803950761	31.143248591118137
2	8.12584163041793	6.497338054813752
3	2.827929593685671	3.391774428006459
4	1.5609843746707828	2.4962935882363446
5	1.1491097568042223	2.2970403841287537
6	0.911181436306298	2.185713464605734
7	0.8025831290987551	2.246079791370267
8	0.7550979598331524	2.415073752444685
9	0.6604730638306291	2.376483120325666
>10	5.222276570431089	34.72650199393128
>50	0.058361719552963064	1.5656544126532814
>100	0.0214239223675434	1.508651714739287
>500	0.002955023774833573	0.7342420052548685
>1k	0.002955023774833573	3.3829476782740397
>5k	0.0	0.0
>10k+	7.387559437083932E-4	3.0329570200974434
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10269	3.022223007036844	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCG	3712	1.0924619536586586	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTC	3404	1.0018158648313777	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGC	2995	0.8814449222003455	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	1343	0.39525226394492957	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCT	690	0.20307078341176574	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCT	668	0.19659606278124567	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTA	576	0.16951995832634356	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTC	552	0.16245662672941258	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCC	496	0.14597551966990696	No Hit
CTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGCT	361	0.10624427943717019	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCTTCTGTTCGTATGCCGTCTTCTGC	356	0.1047727520211429	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.829164496163728E-4	0.0	0.0	0.11477913845012847	0.0
2	8.829164496163728E-4	0.0	0.0	0.7919760553058864	0.0
3	8.829164496163728E-4	0.0	0.0	0.9715024000612156	0.0
4	8.829164496163728E-4	0.0	0.0	1.4150207632518401	0.0
5	8.829164496163728E-4	0.0	0.0	3.1732017199212437	0.0
6	8.829164496163728E-4	0.0	0.0	3.6361442450034285	0.0
7	8.829164496163728E-4	0.0	0.0	4.3089265796111045	0.0
8	8.829164496163728E-4	0.0	0.0	5.190665807294656	0.0
9	8.829164496163728E-4	0.0	0.0	5.515284755270275	0.0
10	8.829164496163728E-4	0.0	0.0	7.381475824276082	0.0
11	8.829164496163728E-4	0.0	0.0	8.425083067722635	0.0
12	8.829164496163728E-4	0.0	0.0	10.185618468257681	0.0
13	8.829164496163728E-4	0.0	0.0	10.59264295153083	0.0
14	8.829164496163728E-4	0.0	0.0	10.771580685319748	0.0
15	8.829164496163728E-4	0.0	0.0	11.22245668559051	0.0
16	8.829164496163728E-4	0.0	0.0	11.829608897443368	0.0
17	8.829164496163728E-4	0.0	0.0	12.513869145896058	0.0
18	8.829164496163728E-4	0.0	0.0	13.371769629439965	0.0
19	8.829164496163728E-4	0.0	0.0	13.839126736770233	0.0
20	8.829164496163728E-4	0.0	0.0	14.249682885841846	0.0
21	0.0011772219328218304	0.0	0.0	14.717039993172113	0.0
22	0.0011772219328218304	0.0	0.0	15.218536536554213	0.0
23	0.0011772219328218304	0.0	0.0	15.72150460735234	0.0
24	0.0011772219328218304	0.0	0.0	16.130000618041514	0.0
25	0.0011772219328218304	0.0	0.0	16.507300247510912	0.0
26	0.0011772219328218304	0.0	0.0	16.857523772525408	0.0
27	0.0011772219328218304	0.0	0.0	17.235706318444418	0.0
28	0.001471527416027288	0.0	0.0	17.5744519296139	0.0
29	0.001471527416027288	0.0	0.0	17.935564757507	0.0
30	0.001471527416027288	0.0	0.0	18.402333253870854	0.0
31	0.0017658328992327455	0.0	0.0	18.80435454392951	0.0
32	0.0017658328992327455	0.0	0.0	19.213439165585093	0.0
33	0.0017658328992327455	0.0	0.0	19.627232674971967	0.0
34	0.0017658328992327455	0.0	0.0	20.001883555092515	0.0
35	0.0017658328992327455	0.0	0.0	20.42009164672747	0.0
36	0.0017658328992327455	0.0	0.0	20.8153439106724	0.0
37	0.0017658328992327455	0.0	0.0	21.187934652410508	0.0
38	0.0017658328992327455	0.0	0.0	21.590544553435574	0.0
39	0.0017658328992327455	0.0	0.0	22.02817680696209	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	35	1.2085547E-7	45.000004	5
GCCAACG	35	1.2085547E-7	45.000004	1
CGGGTAT	20	7.026265E-4	45.0	6
TTAGGCG	20	7.026265E-4	45.0	1
CTCACGA	25	3.884986E-5	45.0	6
TTGGACG	20	7.026265E-4	45.0	1
CGAAAGG	20	7.026265E-4	45.0	2
ATGTGCG	25	3.884986E-5	45.0	1
GACGTAG	20	7.026265E-4	45.0	1
TATATCG	20	7.026265E-4	45.0	18
CACGATC	20	7.026265E-4	45.0	8
CGATTCG	20	7.026265E-4	45.0	23
CTACGAC	20	7.026265E-4	45.0	5
GCGATAC	40	6.7902874E-9	45.0	9
TCGTAAG	20	7.026265E-4	45.0	1
ACGTAGG	40	6.7902874E-9	45.0	2
TAGGCGG	25	3.884986E-5	45.0	2
ACGTAAG	25	3.884986E-5	45.0	1
CCAACGG	40	6.7902874E-9	45.0	2
CGATCAT	20	7.026265E-4	45.0	10
>>END_MODULE