FastQCFastQC Report
Sat 18 Jun 2016
SRR3552201_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552201_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277111
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76092.7458310929555303No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG39371.4207303210626787No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC37031.336287624814605No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC34521.2457102027707307Illumina PCR Primer Index 7 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC13240.4777868796258539Illumina PCR Primer Index 7 (96% over 27bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTA9810.35400976504000203No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC8720.314675346702224No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGCATCT8620.31106668446940033No Hit
GAACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT7550.2724539985781871Illumina PCR Primer Index 7 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT7230.26090627943315137Illumina PCR Primer Index 7 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTC6240.22518052332819702Illumina PCR Primer Index 7 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT4670.16852452627286538Illumina PCR Primer Index 7 (96% over 28bp)
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC4000.1443464893129468Illumina PCR Primer Index 7 (96% over 27bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATG2790.1006816762957804No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTCG207.0240116E-445.040
CGACGGT207.0240116E-445.028
CGTGCGG406.7830115E-945.02
TATGCGG207.0240116E-445.02
CGGGCTA207.0240116E-445.06
GTGTACG207.0240116E-445.01
CGAATAT207.0240116E-445.014
AGTTAAC207.0240116E-445.043
CGGCTAT453.8198777E-1045.031
CGCAATC406.7830115E-945.020
TGTACGG351.2074634E-745.02
AATCGCG207.0240116E-445.01
AGGGCGC302.1595188E-644.9999966
CGTTTAT302.1595188E-644.99999639
CGTTTTT35700.043.7394941
ATGGGTA650.041.538465
CGATGAA3250.040.84615319
ACGGCTG4700.040.691498
TAGCCGT501.0750227E-940.544
CCGATGA3300.040.22727618