##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552201_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	277111
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.122131564607685	28.0	16.0	31.0	16.0	33.0
2	26.24130402618445	30.0	25.0	31.0	16.0	33.0
3	26.620668252072274	30.0	23.0	33.0	16.0	34.0
4	29.904204452367463	32.0	28.0	35.0	19.0	37.0
5	32.008877309092746	33.0	32.0	35.0	28.0	37.0
6	30.454384705045992	35.0	28.0	35.0	17.0	37.0
7	32.884125855704035	35.0	32.0	35.0	28.0	37.0
8	33.283821284611584	35.0	33.0	37.0	28.0	37.0
9	35.095405812111395	37.0	34.0	39.0	30.0	39.0
10	34.9631699932518	37.0	34.0	39.0	30.0	39.0
11	34.924882086961546	37.0	34.0	39.0	28.0	39.0
12	34.73384311701809	37.0	33.0	39.0	27.0	39.0
13	34.97965436233134	37.0	34.0	39.0	27.0	39.0
14	35.13819372020598	38.0	33.0	40.0	26.0	41.0
15	35.8333736300616	38.0	34.0	40.0	27.0	41.0
16	35.61846335944802	37.0	33.0	40.0	27.0	41.0
17	35.325548246009724	37.0	33.0	40.0	27.0	41.0
18	35.18923824748927	37.0	33.0	39.0	27.0	40.0
19	34.48380973689244	37.0	32.0	39.0	26.0	40.0
20	35.05721173103919	36.0	34.0	39.0	29.0	40.0
21	35.48655953751385	37.0	34.0	39.0	30.0	40.0
22	36.17083046143964	38.0	35.0	40.0	31.0	40.0
23	36.608499843023196	38.0	35.0	40.0	32.0	41.0
24	36.57128731807831	38.0	35.0	40.0	32.0	41.0
25	34.83080787121406	36.0	33.0	40.0	26.0	41.0
26	35.40574354680976	37.0	34.0	40.0	30.0	41.0
27	35.89140452742764	37.0	35.0	40.0	31.0	41.0
28	35.96436446045087	38.0	35.0	40.0	30.0	41.0
29	36.09478873086958	38.0	35.0	40.0	31.0	41.0
30	35.035570583628946	37.0	34.0	40.0	27.0	41.0
31	35.030283893457856	37.0	34.0	40.0	27.0	41.0
32	34.81738003904572	37.0	34.0	40.0	26.0	41.0
33	34.828036418619256	38.0	34.0	40.0	24.0	41.0
34	34.667212777551235	38.0	34.0	40.0	23.0	41.0
35	34.549660605317	38.0	34.0	40.0	21.0	41.0
36	34.349477285275576	38.0	34.0	40.0	20.0	41.0
37	34.40219984049713	38.0	34.0	40.0	18.0	41.0
38	34.03584845062087	37.0	34.0	40.0	18.0	41.0
39	33.937068539321785	37.0	34.0	40.0	18.0	41.0
40	33.696991458296495	37.0	33.0	40.0	18.0	41.0
41	33.37576999830393	37.0	33.0	40.0	18.0	41.0
42	33.68376931987543	37.0	33.0	40.0	17.0	41.0
43	33.642013489179426	37.0	33.0	40.0	16.0	41.0
44	33.740905269007726	37.0	33.0	40.0	17.0	41.0
45	33.74188321647282	37.0	34.0	40.0	18.0	41.0
46	33.54822435774834	37.0	33.0	40.0	17.0	41.0
47	33.42655831056869	36.0	33.0	40.0	17.0	41.0
48	33.4356954433422	36.0	33.0	40.0	17.0	41.0
49	33.545687468198665	36.0	33.0	40.0	18.0	41.0
50	33.42933337182573	36.0	33.0	40.0	17.0	41.0
51	32.2731035577801	35.0	31.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	19.0
10	23.0
11	28.0
12	28.0
13	23.0
14	39.0
15	58.0
16	79.0
17	163.0
18	281.0
19	617.0
20	964.0
21	1548.0
22	1957.0
23	2642.0
24	3730.0
25	5446.0
26	7004.0
27	7420.0
28	7495.0
29	7669.0
30	9275.0
31	12239.0
32	16608.0
33	20899.0
34	25243.0
35	36080.0
36	43580.0
37	42785.0
38	21009.0
39	2151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.04167644012688	16.55798578908813	20.218973624287738	22.181364146497252
2	40.521307346153705	24.23938421787659	19.65169192128786	15.587616514681843
3	27.570901191219406	23.130803179953162	33.83481709495473	15.463478533872706
4	24.169737036783097	20.838941795886846	38.78229301615598	16.209028151174078
5	26.16027512440863	23.68112417045877	32.414808506338616	17.743792198793983
6	21.208107942304707	30.69455921995157	33.482972527254425	14.6143603104893
7	64.38394722692351	5.141621949327165	26.534854264175728	3.9395765595736
8	64.32837382853802	9.518207505295711	20.762438156550985	5.3909805096152805
9	59.339759157882575	6.313715442548292	22.127234213004897	12.219291186564227
10	36.392636885580146	20.66825207227429	28.786659497457695	14.15245154468787
11	29.857710448159764	20.758829494318164	32.730205585487404	16.653254472034675
12	23.84892696428507	19.011876107408224	37.26448968103034	19.874707247276362
13	22.405822937378883	21.542630931287462	40.01320770377214	16.03833842756152
14	18.520015445074357	25.714966204878188	37.0977694858739	18.667248864173562
15	16.75682307811671	20.951892923774228	43.66986514429236	18.621418853816703
16	19.996319164522518	20.926993154367747	37.52900462269632	21.547683058413416
17	18.88701639415252	22.12759507922818	38.024834813486294	20.960553713133002
18	21.236616373944013	20.13056139958356	38.19408107220572	20.4387411542667
19	20.296198996070167	22.367931983934238	35.759316663719595	21.576552356276004
20	22.352053870109813	22.28637621747242	39.0071126732609	16.354457239156872
21	20.553496613270493	25.756104954332383	36.40418460472518	17.286213827671943
22	19.678756888034037	19.865685591694305	37.9100793544825	22.54547816578916
23	19.63184427900733	25.013081400593983	36.55466581983393	18.800408500564757
24	20.372341769182746	22.15718610953733	36.5102792743702	20.960192846909724
25	18.22915726910877	25.71785313466445	35.19347842561284	20.859511170613942
26	17.745596529910397	22.47835704825864	37.423270819274585	22.352775602556378
27	20.75738602942503	23.434291673733632	35.08197076261859	20.726351534222747
28	16.046638350697012	25.36853463052712	38.29981487562746	20.285012143148414
29	20.738260119591068	23.071981985558136	35.86613306581118	20.323624829039627
30	20.817289822489904	24.339705027949087	36.59796976662781	18.245035382933192
31	22.083930266211013	25.375751954992765	32.455586389569525	20.0847313892267
32	24.61251989275056	24.57138114329637	32.40614771697984	18.409951246973236
33	21.5664481020241	23.14199003287491	30.640068420235934	24.651493444865054
34	20.640465373081547	27.85021164803996	31.92908256980055	19.580240409077952
35	21.54696132596685	25.00947273836116	32.391713068048546	21.051852867623445
36	21.139904226104342	29.603299760745692	28.937862445013007	20.318933568136956
37	23.3079884955848	25.962520434049896	31.55089476780063	19.178596302564678
38	20.88405007379714	27.770821078917834	28.684895222492067	22.66023362479295
39	23.223184933113444	25.176553799740898	29.301976464304918	22.29828480284074
40	22.347001742983856	23.252415097199318	32.77567472962099	21.62490843019584
41	19.338099173255483	26.00474178217393	30.477317753535587	24.179841291035
42	21.466488158174883	23.676432909556098	32.99363792848353	21.863441003785486
43	22.293232675714787	23.678598106895794	29.242072671240045	24.786096546149377
44	21.758428932810318	24.031525273265945	30.55382139287145	23.656224401052288
45	20.85193297992501	23.997603848277404	31.27483210698962	23.875631064807965
46	23.597042340433976	25.248366178174088	29.86673210374182	21.28785937765011
47	19.28757790199595	25.02462911973902	35.17110471976934	20.516688258495694
48	20.77254241080289	25.267852954231333	31.643998253407478	22.315606381558293
49	21.527474549909602	22.54223036977962	34.451537470544295	21.478757609766483
50	20.464723522343032	23.261436752781375	32.570341848573314	23.703497876302276
51	19.74623887178784	23.432126476393936	30.25502415999365	26.566610491824576
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	318.0
1	645.5
2	973.0
3	6784.0
4	12595.0
5	8088.5
6	3582.0
7	3470.0
8	3358.0
9	3285.5
10	3213.0
11	3112.0
12	3011.0
13	2881.0
14	2751.0
15	2571.5
16	2392.0
17	2209.0
18	2026.0
19	1958.0
20	1890.0
21	1833.0
22	1776.0
23	1756.5
24	1737.0
25	1784.5
26	2196.0
27	2560.0
28	2699.5
29	2839.0
30	2948.0
31	3057.0
32	3967.0
33	4877.0
34	5322.0
35	5767.0
36	5810.5
37	5854.0
38	6564.5
39	7275.0
40	7927.5
41	8580.0
42	10332.5
43	12085.0
44	12958.0
45	13831.0
46	22449.0
47	31067.0
48	26641.5
49	22216.0
50	21510.5
51	20805.0
52	19233.5
53	17662.0
54	16291.5
55	14921.0
56	13888.0
57	12855.0
58	12272.0
59	11689.0
60	10696.0
61	9703.0
62	9130.5
63	8558.0
64	7897.0
65	7236.0
66	5903.0
67	4570.0
68	3788.5
69	3007.0
70	2549.0
71	2091.0
72	1659.5
73	1228.0
74	955.0
75	506.5
76	331.0
77	247.0
78	163.0
79	118.0
80	73.0
81	60.5
82	48.0
83	31.5
84	15.0
85	11.0
86	7.0
87	5.5
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	277111.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.05005948680597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.47702100875074	28.338550797830635
2	8.86544717895311	6.74661585072604
3	3.5734597295165047	4.0791106584542565
4	2.22966017285967	3.3935480885069014
5	1.5930555346938304	3.030792893044287
6	1.2416432360487384	2.8346759395826857
7	1.1178702618049006	2.977452097214548
8	0.9521849642317327	2.898455562516772
9	0.7924892597954895	2.7138837130085887
>10	5.064345606215017	30.336555548477516
>50	0.0578896422962991	1.53847504364856
>100	0.02395433474329618	1.6774732825958256
>500	0.005988583685824045	1.8293839262540394
>1k	0.0039923891238826965	4.715306379150955
>5k	9.980972809706741E-4	2.8897202189883715
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7609	2.7458310929555303	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG	3937	1.4207303210626787	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC	3703	1.336287624814605	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	3452	1.2457102027707307	Illumina PCR Primer Index 7 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	1324	0.4777868796258539	Illumina PCR Primer Index 7 (96% over 27bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTA	981	0.35400976504000203	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC	872	0.314675346702224	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGCATCT	862	0.31106668446940033	No Hit
GAACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT	755	0.2724539985781871	Illumina PCR Primer Index 7 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT	723	0.26090627943315137	Illumina PCR Primer Index 7 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTC	624	0.22518052332819702	Illumina PCR Primer Index 7 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT	467	0.16852452627286538	Illumina PCR Primer Index 7 (96% over 28bp)
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	400	0.1443464893129468	Illumina PCR Primer Index 7 (96% over 27bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATG	279	0.1006816762957804	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18079397786446585	0.0
2	0.0	0.0	0.0	0.9631519499406375	0.0
3	0.0	0.0	0.0	1.181836881249752	0.0
4	0.0	0.0	0.0	1.771853156316422	0.0
5	0.0	0.0	0.0	3.7212524944877683	0.0
6	0.0	0.0	0.0	4.184604725182328	0.0
7	0.0	0.0	0.0	4.905254573077214	0.0
8	0.0	0.0	0.0	5.793707214798402	0.0
9	0.0	0.0	0.0	6.091060982783072	0.0
10	0.0	0.0	0.0	8.220893432595602	0.0
11	0.0	0.0	0.0	9.408504173417873	0.0
12	0.0	0.0	0.0	11.677630985417396	0.0
13	0.0	0.0	0.0	12.139900617442109	0.0
14	0.0	0.0	0.0	12.341985702480233	0.0
15	0.0	0.0	0.0	12.96953206476827	0.0
16	0.0	0.0	0.0	13.498201081876937	0.0
17	0.0	0.0	0.0	14.101930273428337	0.0
18	0.0	0.0	0.0	14.778915308306058	0.0
19	0.0	0.0	0.0	15.45445687829065	0.0
20	0.0	0.0	0.0	15.906622256063455	0.0
21	0.0	0.0	0.0	16.318731483051916	0.0
22	0.0	0.0	0.0	16.773062058164417	0.0
23	0.0	0.0	0.0	17.151249860164338	0.0
24	0.0	0.0	0.0	17.459790481070762	0.0
25	0.0	0.0	0.0	17.763639841074514	0.0
26	0.0	0.0	0.0	18.04078510055537	0.0
27	0.0	0.0	0.0	18.348964855238513	0.0
28	0.0	0.0	0.0	18.61636672669075	0.0
29	0.0	0.0	0.0	18.901451043083817	0.0
30	0.0	0.0	0.0	19.233447968503597	0.0
31	0.0	0.0	0.0	19.536936462284068	0.0
32	0.0	0.0	0.0	19.835733695161867	0.0
33	0.0	0.0	0.0	20.135613526709513	0.0
34	0.0	0.0	0.0	20.437297689373572	0.0
35	0.0	0.0	0.0	20.744394845386868	0.0
36	0.0	0.0	0.0	21.046079008050924	0.0
37	0.0	0.0	0.0	21.34271104358903	0.0
38	0.0	0.0	0.0	21.666408045873315	0.0
39	0.0	0.0	0.0	22.03268726250492	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCCTCG	20	7.0240116E-4	45.0	40
CGACGGT	20	7.0240116E-4	45.0	28
CGTGCGG	40	6.7830115E-9	45.0	2
TATGCGG	20	7.0240116E-4	45.0	2
CGGGCTA	20	7.0240116E-4	45.0	6
GTGTACG	20	7.0240116E-4	45.0	1
CGAATAT	20	7.0240116E-4	45.0	14
AGTTAAC	20	7.0240116E-4	45.0	43
CGGCTAT	45	3.8198777E-10	45.0	31
CGCAATC	40	6.7830115E-9	45.0	20
TGTACGG	35	1.2074634E-7	45.0	2
AATCGCG	20	7.0240116E-4	45.0	1
AGGGCGC	30	2.1595188E-6	44.999996	6
CGTTTAT	30	2.1595188E-6	44.999996	39
CGTTTTT	3570	0.0	43.739494	1
ATGGGTA	65	0.0	41.53846	5
CGATGAA	325	0.0	40.846153	19
ACGGCTG	470	0.0	40.69149	8
TAGCCGT	50	1.0750227E-9	40.5	44
CCGATGA	330	0.0	40.227276	18
>>END_MODULE