FastQCFastQC Report
Sat 18 Jun 2016
SRR3552200_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552200_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301763
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83572.769391873755232No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG54251.7977684474239717No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC50091.6599119176307235Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC47251.5657983251757175No Hit
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC14490.48017815305388667Illumina PCR Primer Index 7 (96% over 27bp)
GAATGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT10650.3529259717062728Illumina PCR Primer Index 7 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT10200.3380136067045993Illumina PCR Primer Index 7 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTA9630.3191246110358129No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTC8790.2912881963660223Illumina PCR Primer Index 7 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC8530.2826721632539443No Hit
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT4760.157739683128813Illumina PCR Primer Index 7 (96% over 28bp)
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC4030.13354851323720934Illumina PCR Primer Index 7 (96% over 27bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGCATCT3220.10670625623419705No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCCG253.8839455E-545.00000412
CAATTGG253.8839455E-545.0000042
GCCAGTA1050.045.00000425
CGGAATG207.0250104E-445.06
TCGTTTA351.2079545E-745.038
CGAACGG207.0250104E-445.02
ACGTTAG207.0250104E-445.01
CGCATCG351.2079545E-745.021
ATAGCGG406.7866495E-945.02
CTAAGTA207.0250104E-445.040
TATGCGG351.2079545E-745.02
CGTTAGG406.7866495E-945.02
AGGTTGC207.0250104E-445.010
CACTAGA207.0250104E-445.024
GCGATTT207.0250104E-445.09
GCGATAT351.2079545E-745.09
ATGGTCG351.2079545E-745.044
CTCTCTA207.0250104E-445.015
CGATAGA207.0250104E-445.010
CACCCGT207.0250104E-445.016