##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552200_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	301763
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.845892306213816	30.0	25.0	33.0	16.0	33.0
2	28.526303754933508	31.0	28.0	33.0	16.0	34.0
3	28.354788360402036	31.0	27.0	33.0	16.0	34.0
4	32.539042228503824	35.0	32.0	35.0	28.0	37.0
5	33.707740842979426	35.0	33.0	35.0	30.0	37.0
6	33.422258527387385	35.0	33.0	36.0	28.0	37.0
7	34.329102640151376	35.0	35.0	37.0	32.0	37.0
8	34.361088006150524	35.0	35.0	37.0	31.0	37.0
9	36.25913050970463	39.0	35.0	39.0	31.0	39.0
10	36.07099279898463	37.0	35.0	39.0	31.0	39.0
11	36.08316460268489	37.0	35.0	39.0	31.0	39.0
12	35.902622256539075	37.0	35.0	39.0	31.0	39.0
13	36.025569735189535	37.0	35.0	39.0	31.0	39.0
14	37.101052812969115	39.0	36.0	40.0	32.0	41.0
15	37.21842969482673	39.0	36.0	40.0	32.0	41.0
16	37.09472665634952	39.0	35.0	40.0	32.0	41.0
17	36.11570006925965	38.0	34.0	40.0	29.0	41.0
18	35.877625818937375	38.0	34.0	39.0	30.0	41.0
19	35.30702571223112	37.0	34.0	39.0	28.0	41.0
20	35.74302681243228	37.0	34.0	39.0	30.0	40.0
21	36.227314150508846	38.0	35.0	40.0	31.0	41.0
22	36.67369425675116	38.0	35.0	40.0	32.0	41.0
23	36.91417436862704	38.0	35.0	40.0	33.0	41.0
24	36.841372202688866	38.0	35.0	40.0	33.0	41.0
25	36.13748869145654	38.0	35.0	40.0	31.0	41.0
26	36.30387754628632	38.0	35.0	40.0	31.0	41.0
27	36.65490799070794	38.0	35.0	40.0	32.0	41.0
28	36.568230697600434	38.0	35.0	40.0	32.0	41.0
29	36.57441767214669	38.0	35.0	40.0	32.0	41.0
30	36.1670947067732	38.0	35.0	40.0	31.0	41.0
31	36.00817528987981	38.0	35.0	40.0	30.0	41.0
32	35.80103591228878	38.0	35.0	40.0	30.0	41.0
33	35.42039282483273	38.0	35.0	40.0	26.0	41.0
34	35.04304371311261	38.0	35.0	40.0	23.0	41.0
35	34.927148126178494	38.0	35.0	40.0	22.0	41.0
36	34.64043305507965	38.0	35.0	40.0	21.0	41.0
37	34.59648465849027	38.0	35.0	40.0	20.0	41.0
38	34.38642245735892	38.0	34.0	40.0	18.0	41.0
39	34.20169470743597	38.0	34.0	40.0	18.0	41.0
40	33.995214787763906	38.0	34.0	40.0	18.0	41.0
41	33.74976057369525	37.0	33.0	40.0	16.0	41.0
42	33.80020413370758	37.0	33.0	40.0	15.0	41.0
43	33.74053810440644	37.0	33.0	40.0	15.0	41.0
44	33.61062489437075	37.0	33.0	40.0	15.0	41.0
45	33.55736455430255	37.0	33.0	40.0	15.0	41.0
46	33.33435179263196	37.0	33.0	40.0	15.0	41.0
47	33.243658102550675	36.0	33.0	40.0	15.0	41.0
48	33.21122536560148	36.0	33.0	40.0	15.0	41.0
49	33.21279944857388	36.0	33.0	40.0	15.0	41.0
50	33.12323247051494	36.0	33.0	40.0	15.0	41.0
51	31.12740793271541	35.0	29.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	14.0
10	15.0
11	13.0
12	7.0
13	14.0
14	24.0
15	36.0
16	89.0
17	163.0
18	356.0
19	498.0
20	734.0
21	1156.0
22	1691.0
23	2289.0
24	3499.0
25	5300.0
26	7125.0
27	8064.0
28	7978.0
29	7636.0
30	7938.0
31	9381.0
32	12822.0
33	18256.0
34	25136.0
35	29328.0
36	36574.0
37	50844.0
38	50661.0
39	14115.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.3493370625292	16.601770263418643	19.965999807796184	22.082892866255968
2	39.766638057018255	26.907540023130732	19.46096771307284	13.864854206778169
3	26.936701981356233	25.783147702004555	33.829859856907575	13.450290459731642
4	24.095067983815113	20.52604196008126	39.55421970221664	15.82467035388699
5	25.678098375214987	23.50917773219381	32.653108565331074	18.159615327260134
6	20.727193194659385	32.54176290665191	33.952472635810224	12.778571262878485
7	64.34287835155403	5.420478985163854	26.227867564943345	4.008775098338763
8	63.58466743769117	9.790133316543114	21.588465119978263	5.036734125787456
9	58.8985395823875	6.723488300421192	22.547164496641404	11.830807620549901
10	35.435093102865494	20.753372679884546	29.373051036740755	14.438483180509207
11	28.766283474117106	20.805731650334867	33.229720012062444	17.198264863485583
12	24.272359434390562	19.063304646361548	37.89165669747451	18.772679221773377
13	21.1695933563757	21.63684745976147	41.40037048942382	15.793188694439014
14	18.65172337231536	25.73509674811027	37.143718746168354	18.469461133406018
15	16.72206334109881	21.14739050181765	44.81795316191846	17.31259299516508
16	19.71282098865666	21.53842585075042	37.67062230956081	21.0781308510321
17	18.682873645874412	21.07316006269821	38.831135692579934	21.41283059884744
18	21.032399598360303	20.926687499792884	38.56470143788337	19.47621146396344
19	20.001126712022348	23.04424333003052	35.77874026968183	21.175889688265297
20	21.282264558610564	23.128415345817743	39.08497728349731	16.504342812074377
21	20.386859886732303	25.933265509688063	36.572740859548716	17.10713374403091
22	19.40131825306615	20.281810559942738	36.97073531214894	23.346135874842176
23	18.62653804475698	25.87659852268171	36.94621275636841	18.550650676192905
24	20.67516561009799	21.32998412661592	36.35004954219039	21.644800721095695
25	18.07378638202828	26.832646812233442	34.88664945669284	20.20691734904544
26	17.690704294429736	22.62835403942829	36.7593111150141	22.921630551127873
27	20.473682989630937	23.68050423676859	34.727915615897246	21.11789715770323
28	15.919115332230923	24.481795316191846	39.016711790378544	20.58237756119869
29	21.233219447049507	23.627813880429343	34.78889061945964	20.350076053061507
30	21.135129223927386	24.067894340923175	36.62012904166515	18.17684739348429
31	22.285038258500876	25.809327187229712	32.196458810390936	19.709175743878475
32	24.841680391565564	24.758171147556197	32.22230690972717	18.17784155115107
33	20.811696596335537	23.26229524494388	30.555104502540072	25.37090365618051
34	20.87134605634223	28.68973333377518	31.991993716923545	18.446926892959045
35	21.516554382081303	24.805559329672626	32.5377862759848	21.14010001226128
36	20.976726769020722	29.61993352399068	28.79610820412045	20.607231502868146
37	23.72590410355146	25.884220398127006	31.617527662437077	18.772347835884453
38	20.362668716840698	27.71810990744392	28.610532106321852	23.308689269393533
39	23.737502609663874	25.05840676292322	29.27131556884045	21.932775058572457
40	22.115368683370725	23.059818466810047	33.64328960144219	21.181523248377037
41	19.198841474932316	26.76007330255863	29.98843463247648	24.052650590032577
42	21.39924377740147	23.571809665200835	33.87757942491293	21.151367132484765
43	22.872585439566812	23.31034619883816	28.93330196213585	24.88376639945918
44	21.578854929199405	24.150078041376844	30.648555323217224	23.622511706206524
45	20.893548910900275	23.510171889860587	31.343140146406284	24.253139052832854
46	23.954560366910457	25.132637202042662	29.8717867995745	21.041015631472384
47	19.335703847058785	24.0135470551393	36.11310863160825	20.53764046619367
48	20.996941308245212	24.88774303012629	31.55489572943005	22.560419932198446
49	20.92966997279322	22.315857146171002	35.01158193681796	21.742890944217812
50	20.64037009176075	22.73340336621786	33.11936851105006	23.506858030971326
51	20.11578622959077	22.829505274006422	30.112041569045907	26.9426669273569
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	481.0
1	800.5
2	1120.0
3	7471.5
4	13823.0
5	8891.0
6	3959.0
7	3778.5
8	3598.0
9	3570.0
10	3542.0
11	3465.5
12	3389.0
13	3311.5
14	3234.0
15	3003.0
16	2772.0
17	2603.5
18	2435.0
19	2259.5
20	2084.0
21	2113.0
22	2142.0
23	1996.0
24	1850.0
25	1961.5
26	2437.0
27	2801.0
28	2907.5
29	3014.0
30	3203.0
31	3392.0
32	4387.5
33	5383.0
34	5830.5
35	6278.0
36	6190.0
37	6102.0
38	7068.0
39	8034.0
40	8814.0
41	9594.0
42	11466.5
43	13339.0
44	14524.5
45	15710.0
46	26815.0
47	37920.0
48	30491.5
49	23063.0
50	22422.0
51	21781.0
52	20171.0
53	18561.0
54	16834.5
55	15108.0
56	14067.0
57	13026.0
58	12648.5
59	12271.0
60	11235.5
61	10200.0
62	9653.5
63	9107.0
64	8409.5
65	7712.0
66	6269.5
67	4827.0
68	3994.0
69	3161.0
70	2738.5
71	2316.0
72	1803.0
73	1290.0
74	972.5
75	479.0
76	303.0
77	225.0
78	147.0
79	109.0
80	71.0
81	54.0
82	37.0
83	33.5
84	30.0
85	21.5
86	13.0
87	10.0
88	7.0
89	4.5
90	2.0
91	2.0
92	2.0
93	2.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	301763.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.922668388028605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.39623284320649	25.981149689018622
2	8.779997225446214	6.1324186310413875
3	3.22890426825225	3.3828585905059043
4	2.0423599175048315	2.8529863251209076
5	1.4974517675318315	2.6147505752290723
6	1.2179976239291856	2.5521436270731437
7	1.0493969295110572	2.5653418683711053
8	0.884802976819123	2.4719744758555833
9	0.7900379600577008	2.48312103237447
>10	6.0010959568227165	34.99604885093438
>50	0.07381667221406632	1.6944303788818411
>100	0.027930632729646715	1.9107628758566815
>500	0.002992567792462148	0.9388342662595728
>1k	0.003990090389949531	2.8771177013127316
>5k	0.002992567792462148	6.546061112164613
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8357	2.769391873755232	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG	5425	1.7977684474239717	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	5009	1.6599119176307235	Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC	4725	1.5657983251757175	No Hit
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	1449	0.48017815305388667	Illumina PCR Primer Index 7 (96% over 27bp)
GAATGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT	1065	0.3529259717062728	Illumina PCR Primer Index 7 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT	1020	0.3380136067045993	Illumina PCR Primer Index 7 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTA	963	0.3191246110358129	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTC	879	0.2912881963660223	Illumina PCR Primer Index 7 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC	853	0.2826721632539443	No Hit
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT	476	0.157739683128813	Illumina PCR Primer Index 7 (96% over 28bp)
CCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	403	0.13354851323720934	Illumina PCR Primer Index 7 (96% over 27bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGCATCT	322	0.10670625623419705	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1815994671314906	0.0
2	0.0	0.0	0.0	1.0080758741131286	0.0
3	0.0	0.0	0.0	1.2281161043600441	0.0
4	0.0	0.0	0.0	1.880283533766565	0.0
5	0.0	0.0	0.0	4.414060040495356	0.0
6	0.0	0.0	0.0	4.914784118662659	0.0
7	0.0	0.0	0.0	5.722702915864437	0.0
8	0.0	0.0	0.0	6.69233802686214	0.0
9	0.0	0.0	0.0	6.993899185784871	0.0
10	0.0	0.0	0.0	9.539936970403927	0.0
11	0.0	0.0	0.0	10.654719100751251	0.0
12	0.0	0.0	0.0	13.143427126586095	0.0
13	0.0	0.0	0.0	13.588809761302745	0.0
14	0.0	0.0	0.0	13.798908414881877	0.0
15	0.0	0.0	0.0	14.391426384281704	0.0
16	0.0	0.0	0.0	14.92926568200873	0.0
17	0.0	0.0	0.0	15.497592481516952	0.0
18	0.0	0.0	0.0	16.165003661814072	0.0
19	0.0	0.0	0.0	16.62993806397736	0.0
20	0.0	0.0	0.0	17.02428727179939	0.0
21	0.0	0.0	0.0	17.42559558328887	0.0
22	0.0	0.0	0.0	17.83817101500184	0.0
23	3.313858889260777E-4	0.0	0.0	18.218933401377903	0.0
24	3.313858889260777E-4	0.0	0.0	18.527453663968082	0.0
25	3.313858889260777E-4	0.0	0.0	18.79123683155324	0.0
26	3.313858889260777E-4	0.0	0.0	19.033479916358203	0.0
27	3.313858889260777E-4	0.0	0.0	19.319797324390333	0.0
28	3.313858889260777E-4	0.0	0.0	19.57927247541945	0.0
29	3.313858889260777E-4	0.0	0.0	19.851008904338837	0.0
30	3.313858889260777E-4	0.0	0.0	20.186371423932027	0.0
31	3.313858889260777E-4	0.0	0.0	20.465066956518857	0.0
32	3.313858889260777E-4	0.0	0.0	20.73779754310502	0.0
33	3.313858889260777E-4	0.0	0.0	21.03372514191601	0.0
34	3.313858889260777E-4	0.0	0.0	21.30479879905754	0.0
35	3.313858889260777E-4	0.0	0.0	21.599732240201746	0.0
36	3.313858889260777E-4	0.0	0.0	21.867160652565094	0.0
37	3.313858889260777E-4	0.0	0.0	22.14121678270696	0.0
38	3.313858889260777E-4	0.0	0.0	22.44940565940821	0.0
39	3.313858889260777E-4	0.0	0.0	22.807302419448376	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCCG	25	3.8839455E-5	45.000004	12
CAATTGG	25	3.8839455E-5	45.000004	2
GCCAGTA	105	0.0	45.000004	25
CGGAATG	20	7.0250104E-4	45.0	6
TCGTTTA	35	1.2079545E-7	45.0	38
CGAACGG	20	7.0250104E-4	45.0	2
ACGTTAG	20	7.0250104E-4	45.0	1
CGCATCG	35	1.2079545E-7	45.0	21
ATAGCGG	40	6.7866495E-9	45.0	2
CTAAGTA	20	7.0250104E-4	45.0	40
TATGCGG	35	1.2079545E-7	45.0	2
CGTTAGG	40	6.7866495E-9	45.0	2
AGGTTGC	20	7.0250104E-4	45.0	10
CACTAGA	20	7.0250104E-4	45.0	24
GCGATTT	20	7.0250104E-4	45.0	9
GCGATAT	35	1.2079545E-7	45.0	9
ATGGTCG	35	1.2079545E-7	45.0	44
CTCTCTA	20	7.0250104E-4	45.0	15
CGATAGA	20	7.0250104E-4	45.0	10
CACCCGT	20	7.0250104E-4	45.0	16
>>END_MODULE