FastQCFastQC Report
Sat 18 Jun 2016
SRR3552198_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552198_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603398
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261794.338595752720427No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCG33340.5525374628354751No Hit
GAATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTC33180.5498858133437632No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGC27170.45028322931133347No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCC18830.31206599955584874No Hit
GCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC15770.2613532030268579No Hit
GAACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCT9120.15114402102758048No Hit
GAATGACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCT7550.12512471039015707No Hit
CTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGCT7000.11600966526239728TruSeq Adapter, Index 15 (95% over 23bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCG6680.1107063662789734No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGC6660.11037491009250942No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTC6110.10125986496474963No Hit
CCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC6100.10109413687151765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAT351.2106466E-745.04
TAACGCC406.8066583E-945.012
GCCGATA207.03062E-445.09
GTATGCG207.03062E-445.01
ACCGTCC207.03062E-445.027
CCAGTCG406.8066583E-945.028
CGTTTTT117800.043.6247861
ATTGCGG556.002665E-1140.9090922
ATAACGC451.925764E-840.011
CGGTAGT451.925764E-840.012
GCGTAAG451.925764E-840.01
AGCCGTT1250.039.645
CACGACC1050.038.5714327
AGGTATA356.2433573E-638.5714266
TAGCCGT1300.038.0769244
GTTTTTT140250.037.9251332
AAGTACG301.1392791E-437.5000041
CCGCTTG301.1392791E-437.50000439
TCGATGG301.1392791E-437.5000042
ATCGTTC301.1392791E-437.50000428