##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552198_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	603398
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.91225691831925	30.0	25.0	33.0	16.0	33.0
2	28.714283109987107	31.0	28.0	33.0	16.0	34.0
3	28.54057355178506	31.0	27.0	33.0	16.0	34.0
4	32.34051654132099	35.0	32.0	35.0	28.0	37.0
5	33.778981700303945	35.0	33.0	35.0	30.0	37.0
6	33.452150984922056	35.0	33.0	36.0	28.0	37.0
7	34.438746896741456	35.0	35.0	37.0	32.0	37.0
8	34.55594980427512	35.0	35.0	37.0	32.0	37.0
9	36.48725716691139	39.0	35.0	39.0	32.0	39.0
10	36.301603585030115	37.0	35.0	39.0	32.0	39.0
11	36.44970981010875	38.0	35.0	39.0	32.0	39.0
12	36.35441449921942	37.0	35.0	39.0	32.0	39.0
13	36.62981978727142	38.0	35.0	39.0	32.0	39.0
14	37.61630300398742	39.0	37.0	40.0	33.0	41.0
15	37.83777042681613	39.0	37.0	41.0	33.0	41.0
16	37.56342082671802	39.0	36.0	41.0	32.0	41.0
17	36.624387220375276	38.0	35.0	40.0	30.0	41.0
18	36.31722014325536	38.0	35.0	39.0	30.0	41.0
19	35.73943897725879	37.0	34.0	39.0	30.0	41.0
20	36.09264366139762	37.0	35.0	39.0	31.0	41.0
21	36.40301260527877	38.0	35.0	40.0	32.0	41.0
22	36.79237750207989	38.0	35.0	40.0	33.0	41.0
23	37.03807437213912	38.0	35.0	40.0	33.0	41.0
24	36.9385645958389	38.0	35.0	40.0	33.0	41.0
25	36.15990275075489	38.0	35.0	40.0	31.0	41.0
26	36.387043046214934	38.0	35.0	40.0	31.0	41.0
27	36.744180458006156	38.0	35.0	40.0	33.0	41.0
28	36.68462606770324	38.0	35.0	40.0	33.0	41.0
29	36.69777493462027	38.0	35.0	40.0	33.0	41.0
30	36.24372304846884	38.0	35.0	40.0	31.0	41.0
31	36.02515089542889	38.0	35.0	40.0	31.0	41.0
32	35.68573976048976	38.0	35.0	40.0	29.0	41.0
33	35.26301877036384	38.0	35.0	40.0	24.0	41.0
34	34.78691013228416	38.0	35.0	40.0	20.0	41.0
35	34.51505805455106	38.0	35.0	40.0	18.0	41.0
36	34.182871007195914	38.0	34.0	40.0	15.0	41.0
37	34.070575308502846	38.0	34.0	40.0	15.0	41.0
38	33.88697178313485	38.0	34.0	40.0	15.0	41.0
39	33.70191150782734	38.0	34.0	40.0	13.0	41.0
40	33.522832359404575	38.0	33.0	40.0	12.0	41.0
41	33.36019509511135	38.0	33.0	40.0	10.0	41.0
42	33.32848468175234	38.0	33.0	40.0	10.0	41.0
43	33.213005346388286	38.0	33.0	40.0	10.0	41.0
44	33.068251800635736	37.0	33.0	40.0	10.0	41.0
45	33.02211972860368	37.0	33.0	40.0	10.0	41.0
46	32.83674788448089	37.0	33.0	40.0	10.0	41.0
47	32.79809512129639	37.0	33.0	40.0	10.0	41.0
48	32.729498606226734	37.0	32.0	40.0	10.0	41.0
49	32.70800201525361	36.0	32.0	40.0	10.0	41.0
50	32.62282108989423	36.0	32.0	40.0	10.0	41.0
51	30.923844626598033	35.0	28.0	38.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	33.0
10	42.0
11	49.0
12	40.0
13	19.0
14	35.0
15	65.0
16	129.0
17	250.0
18	483.0
19	839.0
20	1313.0
21	2051.0
22	3170.0
23	4682.0
24	7568.0
25	12165.0
26	17534.0
27	19773.0
28	18776.0
29	16547.0
30	16224.0
31	17410.0
32	21533.0
33	29773.0
34	40636.0
35	57868.0
36	70035.0
37	105589.0
38	109109.0
39	29646.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.9452666399292	15.91404015260243	19.883725169788434	27.25696803767994
2	45.38397541920921	21.031226487326773	19.247992204150492	14.33680588931352
3	26.89021176735753	19.90245244432365	38.89141163875253	14.315924149566289
4	24.305847881497783	20.463607768007186	39.689060951478126	15.5414833990169
5	21.648563634615957	23.830208253921956	37.427701119327544	17.093526992134546
6	20.527578811994736	28.814149201687776	38.39621609617533	12.262055890142163
7	62.63958448652465	5.7391638686240265	27.60632285821299	4.014928786638338
8	62.524900646008106	6.3047938508248285	26.576322758776133	4.5939827443909325
9	56.82269414217481	7.083384432828746	28.606326172774853	7.487595252221585
10	29.5052353504652	21.541006102108394	34.63783439786012	14.315924149566289
11	23.661994239291477	21.438088956211324	39.03957255410194	15.860344250395261
12	20.98714281452706	19.307985773900477	42.98804437535424	16.71682703621822
13	19.89681768915376	19.649717102144855	44.65659481801398	15.796870390687406
14	17.371121548298138	22.47488390747069	42.764311449491046	17.38968309474012
15	16.471383730141632	20.489792806737842	46.12494572404947	16.91387773907106
16	18.54845392261824	21.062051912667922	42.66785769923003	17.721636465483808
17	17.781961491420255	20.301857149012758	42.440147299129265	19.476034060437723
18	19.439076695646985	20.137123424340153	42.68923662325695	17.734563256755905
19	19.098339735962004	21.441734974262427	41.265300846207644	18.19462444356793
20	20.117898965525242	21.678726147584182	42.0026251329968	16.20074975389378
21	18.962276971418532	23.09818726611623	41.5810128638146	16.358522898650644
22	18.43062124833029	20.186676124216522	40.95008601288039	20.4326166145728
23	17.45730015677878	22.585093089469968	41.393242934182744	18.56436381956851
24	18.089055648179144	21.01664241512236	41.72304183971442	19.17126009698408
25	17.667277650903713	23.107965223616915	40.40633213898621	18.81842498649316
26	16.898962210680182	22.08061677367177	41.583498785213074	19.43692223043497
27	18.22876443077372	21.390027809174043	41.61084392059636	18.770363839455882
28	15.77880602852512	22.774023115754442	42.30209579746701	19.145075058253425
29	18.083752349195723	22.957815571148725	40.69022436269262	18.268207716962934
30	18.170428141956055	22.291920092542565	42.3004385165347	17.237213248966686
31	19.451506302639384	24.043831766098	38.234631205274134	18.270030725988484
32	19.869306825677246	24.200776270388697	37.81368184846486	18.116235055469193
33	18.07115701411009	24.343965342941143	36.65076781825594	20.934109824692822
34	19.00984093417612	25.753151319692808	36.307047752892785	18.929959993238292
35	18.66297203504155	24.858385344333257	35.672474883907476	20.806167736717722
36	18.695786197501484	28.119582762952476	33.99630094895906	19.188330090586973
37	20.17424651722412	26.2166927964627	34.69385049337254	18.915210192940645
38	19.43509922140942	27.659355848047223	32.60053894775919	20.305005982784166
39	21.917871786117953	24.35689213421324	32.84482215718315	20.880413922485655
40	21.005704360969048	24.087749710804477	34.80074511350717	20.105800814719306
41	18.595686429189357	25.03422285125241	33.86073536869529	22.509355350862947
42	19.619057404896935	24.41423405447151	34.179761948166885	21.786946592464677
43	19.872952843728353	24.14923483339355	34.39984885597897	21.577963466899128
44	19.7058989257505	24.90893241276902	34.08778285642312	21.29738580505736
45	20.479351936864227	23.92798782892883	33.80703946648812	21.785620767718818
46	20.496753386653584	24.716853552713133	34.12971206401082	20.656680996622462
47	18.614082247538107	25.084769919688167	36.15441217902611	20.146735653747612
48	18.36018680870669	24.8782727155211	35.07916831013693	21.682372165635286
49	19.408251270305836	23.092055326666642	36.68441062118204	20.815282781845482
50	18.374439424724642	22.750821182701962	36.59640900367585	22.278330388897544
51	18.28444907009967	22.319265227925847	34.666173901802786	24.730111800171695
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	604.0
1	1372.0
2	2140.0
3	21539.5
4	40939.0
5	26411.0
6	11883.0
7	11226.0
8	10569.0
9	10295.5
10	10022.0
11	9492.0
12	8962.0
13	8523.5
14	8085.0
15	7432.5
16	6780.0
17	6556.0
18	6332.0
19	5986.0
20	5640.0
21	5352.5
22	5065.0
23	4865.0
24	4665.0
25	4977.0
26	5954.5
27	6620.0
28	7032.0
29	7444.0
30	8534.5
31	9625.0
32	10746.5
33	11868.0
34	12794.0
35	13720.0
36	14318.5
37	14917.0
38	16276.0
39	17635.0
40	19621.5
41	21608.0
42	24118.0
43	26628.0
44	28835.0
45	31042.0
46	41275.0
47	51508.0
48	49303.0
49	47098.0
50	45577.0
51	44056.0
52	39694.0
53	35332.0
54	31629.0
55	27926.0
56	25722.5
57	23519.0
58	22025.0
59	20531.0
60	19278.5
61	18026.0
62	16351.0
63	14676.0
64	13001.0
65	11326.0
66	9556.0
67	7786.0
68	6289.0
69	4792.0
70	4233.5
71	3675.0
72	3039.0
73	2403.0
74	1810.0
75	930.5
76	644.0
77	494.5
78	345.0
79	271.5
80	198.0
81	143.5
82	89.0
83	77.5
84	66.0
85	54.0
86	42.0
87	33.0
88	24.0
89	19.0
90	14.0
91	9.5
92	5.0
93	9.0
94	13.0
95	8.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	603398.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.87404263106837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.27840541035202	32.70363605393349
2	8.88758511992234	7.620934066375987
3	3.5288243041795306	4.538848909648301
4	2.141474642730827	3.6725470050317344
5	1.589714763259906	3.407874926562199
6	1.2447337914444714	3.2020061783232983
7	1.047668606266481	3.144245194181077
8	0.847600099138559	2.9072034227651486
9	0.7269783053433144	2.805164896963599
>10	3.5872974947350977	23.799766820178053
>50	0.08384121985491884	2.4246076128867258
>100	0.030416814524557977	2.3440214195940157
>500	0.003119673284570049	0.9073469503663852
>1k	0.0019497958028562808	2.1448966328082473
>5k	0.0	0.0
>10k+	3.899591605712561E-4	4.376899910381722
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26179	4.338595752720427	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCG	3334	0.5525374628354751	No Hit
GAATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTC	3318	0.5498858133437632	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGC	2717	0.45028322931133347	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCC	1883	0.31206599955584874	No Hit
GCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	1577	0.2613532030268579	No Hit
GAACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCT	912	0.15114402102758048	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCT	755	0.12512471039015707	No Hit
CTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGCT	700	0.11600966526239728	TruSeq Adapter, Index 15 (95% over 23bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCG	668	0.1107063662789734	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGC	666	0.11037491009250942	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTC	611	0.10125986496474963	No Hit
CCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	610	0.10109413687151765	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6572809323199612E-4	0.0	0.0	0.13606276454346883	0.0
2	1.6572809323199612E-4	0.0	0.0	0.6939035263623677	0.0
3	1.6572809323199612E-4	0.0	0.0	0.9269172254465543	0.0
4	1.6572809323199612E-4	0.0	0.0	1.3917845269623035	0.0
5	1.6572809323199612E-4	0.0	0.0	2.8148916635454544	0.0
6	1.6572809323199612E-4	0.0	0.0	3.4860904411350386	0.0
7	1.6572809323199612E-4	0.0	0.0	4.206676190507758	0.0
8	1.6572809323199612E-4	0.0	0.0	5.217949015409398	0.0
9	1.6572809323199612E-4	0.0	0.0	5.679998939340203	0.0
10	1.6572809323199612E-4	0.0	0.0	7.222264574957159	0.0
11	1.6572809323199612E-4	0.0	0.0	8.648852001498183	0.0
12	1.6572809323199612E-4	0.0	0.0	10.1339414449501	0.0
13	1.6572809323199612E-4	0.0	0.0	10.582401665235881	0.0
14	1.6572809323199612E-4	0.0	0.0	10.782601201860132	0.0
15	1.6572809323199612E-4	0.0	0.0	11.176039695192891	0.0
16	1.6572809323199612E-4	0.0	0.0	11.803486256169228	0.0
17	1.6572809323199612E-4	0.0	0.0	12.51180812664278	0.0
18	3.3145618646399224E-4	0.0	0.0	13.362656157295849	0.0
19	3.3145618646399224E-4	0.0	0.0	13.84857092665206	0.0
20	3.3145618646399224E-4	0.0	0.0	14.339788994991697	0.0
21	3.3145618646399224E-4	0.0	0.0	14.897629756810597	0.0
22	3.3145618646399224E-4	0.0	0.0	15.450332947739303	0.0
23	3.3145618646399224E-4	0.0	0.0	16.00469341960033	0.0
24	3.3145618646399224E-4	0.0	0.0	16.479670134803232	0.0
25	3.3145618646399224E-4	0.0	0.0	16.88321804182314	0.0
26	3.3145618646399224E-4	0.0	0.0	17.274833526130347	0.0
27	3.3145618646399224E-4	0.0	0.0	17.671255125141283	0.0
28	3.3145618646399224E-4	0.0	0.0	18.07364293550857	0.0
29	3.3145618646399224E-4	0.0	0.0	18.495918117063695	0.0
30	3.3145618646399224E-4	0.0	0.0	18.99674841481079	0.0
31	3.3145618646399224E-4	0.0	0.0	19.436259318062042	0.0
32	3.3145618646399224E-4	0.0	0.0	19.880410607923793	0.0
33	4.971842796959884E-4	0.0	0.0	20.32853937202311	0.0
34	4.971842796959884E-4	0.0	0.0	20.772359205698393	0.0
35	4.971842796959884E-4	0.0	0.0	21.2219795226368	0.0
36	4.971842796959884E-4	0.0	0.0	21.67607449809247	0.0
37	4.971842796959884E-4	0.0	0.0	22.106801812402427	0.0
38	4.971842796959884E-4	0.0	0.0	22.549958733704784	0.0
39	4.971842796959884E-4	0.0	0.0	23.032227485009894	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAT	35	1.2106466E-7	45.0	4
TAACGCC	40	6.8066583E-9	45.0	12
GCCGATA	20	7.03062E-4	45.0	9
GTATGCG	20	7.03062E-4	45.0	1
ACCGTCC	20	7.03062E-4	45.0	27
CCAGTCG	40	6.8066583E-9	45.0	28
CGTTTTT	11780	0.0	43.624786	1
ATTGCGG	55	6.002665E-11	40.909092	2
ATAACGC	45	1.925764E-8	40.0	11
CGGTAGT	45	1.925764E-8	40.0	12
GCGTAAG	45	1.925764E-8	40.0	1
AGCCGTT	125	0.0	39.6	45
CACGACC	105	0.0	38.57143	27
AGGTATA	35	6.2433573E-6	38.571426	6
TAGCCGT	130	0.0	38.07692	44
GTTTTTT	14025	0.0	37.925133	2
AAGTACG	30	1.1392791E-4	37.500004	1
CCGCTTG	30	1.1392791E-4	37.500004	39
TCGATGG	30	1.1392791E-4	37.500004	2
ATCGTTC	30	1.1392791E-4	37.500004	28
>>END_MODULE