SRR3552196_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552196_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	440817
Sequences flagged as poor quality	0
Sequence length	51
%GC	37

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18465	4.18881304486896	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCG	6242	1.416007095915085	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTC	5348	1.213201850200877	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGC	4478	1.015841040613225	No Hit
GCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	2011	0.4561983771043313	TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCC	1679	0.38088367735364104	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCT	1451	0.32916153415135985	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCGATACTCGTA	1241	0.2815227180439956	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCT	1165	0.2642820036432352	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTC	1079	0.24477277418974314	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	844	0.19146267045054977	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	777	0.17626361959724784	No Hit
CTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGCT	692	0.15698124164902896	TruSeq Adapter, Index 20 (95% over 22bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	645	0.1463192209011903	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGC	627	0.14223589380627336	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	586	0.13293498209007365	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	564	0.12794424897406406	No Hit
CCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	550	0.12476832790023978	TruSeq Adapter, Index 23 (95% over 21bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	504	0.11433315865767427	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	462	0.1048053954362014	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCGATACT	450	0.10208317737292345	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGCGC	30	2.1624455E-6	45.000004	15
TTGTGCG	20	7.0285506E-4	45.0	1
GTCGAAG	20	7.0285506E-4	45.0	1
CGACGGT	25	3.886879E-5	45.0	28
GCGAAAG	20	7.0285506E-4	45.0	1
CGACAGG	55	1.8189894E-12	45.0	2
GCCGATT	20	7.0285506E-4	45.0	9
GTTAACG	20	7.0285506E-4	45.0	1
GACGGTC	25	3.886879E-5	45.0	29
AACGAAG	20	7.0285506E-4	45.0	1
TTACGCG	20	7.0285506E-4	45.0	1
GCTCGAG	20	7.0285506E-4	45.0	1
TCGACGG	20	7.0285506E-4	45.0	2
GCGTACG	20	7.0285506E-4	45.0	1
ACGACCA	50	2.1827873E-11	45.0	28
GCTCACG	25	3.886879E-5	45.0	44
AACACGT	35	1.2096461E-7	45.0	41
CGTTTTT	7840	0.0	43.478954	1
CGAATAT	100	0.0	42.75	14
GCTACGA	100	0.0	42.75	10

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content