##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552196_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	440817
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.562233307699113	31.0	30.0	33.0	28.0	34.0
2	30.462978968596946	31.0	30.0	33.0	25.0	34.0
3	30.143533484416437	31.0	30.0	34.0	25.0	34.0
4	34.47513140373443	35.0	33.0	37.0	32.0	37.0
5	35.15850568376447	35.0	35.0	37.0	33.0	37.0
6	35.09646860261741	35.0	35.0	37.0	33.0	37.0
7	35.61854011982297	37.0	35.0	37.0	35.0	37.0
8	35.16396599949639	37.0	35.0	37.0	32.0	37.0
9	37.215359208016025	39.0	37.0	39.0	33.0	39.0
10	36.772617662204496	39.0	37.0	39.0	32.0	39.0
11	37.01533062472636	39.0	37.0	39.0	33.0	39.0
12	37.2405034288605	39.0	37.0	39.0	34.0	39.0
13	37.20946787442408	39.0	37.0	39.0	34.0	39.0
14	38.341549894854325	40.0	38.0	41.0	34.0	41.0
15	38.5228609604439	40.0	38.0	41.0	34.0	41.0
16	38.27624615203134	40.0	37.0	41.0	34.0	41.0
17	38.04068354895569	40.0	37.0	41.0	33.0	41.0
18	37.77497918637439	39.0	37.0	40.0	34.0	41.0
19	37.11180603288893	38.0	36.0	40.0	33.0	41.0
20	36.818872684129694	38.0	35.0	40.0	32.0	41.0
21	36.97904799497297	38.0	35.0	40.0	33.0	41.0
22	37.287205348251085	39.0	35.0	40.0	33.0	41.0
23	37.33881406570073	39.0	35.0	40.0	33.0	41.0
24	37.26963116213758	39.0	35.0	41.0	33.0	41.0
25	36.756962639825595	38.0	35.0	40.0	33.0	41.0
26	36.92236007232026	38.0	35.0	40.0	33.0	41.0
27	37.13118595698442	39.0	35.0	41.0	33.0	41.0
28	37.031187544944956	39.0	35.0	41.0	33.0	41.0
29	36.947554200495894	39.0	35.0	41.0	33.0	41.0
30	36.53722746627285	38.0	35.0	41.0	32.0	41.0
31	36.399930129736376	39.0	35.0	41.0	31.0	41.0
32	35.94209842179408	39.0	35.0	41.0	29.0	41.0
33	35.36986323122747	39.0	35.0	41.0	23.0	41.0
34	34.906065328696485	39.0	35.0	41.0	18.0	41.0
35	34.60201398766382	39.0	35.0	41.0	16.0	41.0
36	34.33320629649038	39.0	35.0	41.0	15.0	41.0
37	34.01796663921763	39.0	34.0	41.0	12.0	41.0
38	33.94623846176531	39.0	34.0	41.0	12.0	41.0
39	33.912172171218444	39.0	34.0	41.0	12.0	41.0
40	33.792968510742554	39.0	34.0	41.0	10.0	41.0
41	33.699331014910946	39.0	34.0	41.0	10.0	41.0
42	33.54999240047457	38.0	33.0	41.0	10.0	41.0
43	33.57107371085961	39.0	33.0	41.0	10.0	41.0
44	33.518491800452345	38.0	33.0	41.0	10.0	41.0
45	33.520138742380624	38.0	33.0	40.0	10.0	41.0
46	33.40688085985794	38.0	33.0	40.0	10.0	41.0
47	33.402087487551526	38.0	33.0	40.0	10.0	41.0
48	33.40693984124932	38.0	33.0	40.0	10.0	41.0
49	33.37382859553965	38.0	33.0	40.0	10.0	41.0
50	33.2789933237602	38.0	33.0	40.0	10.0	41.0
51	32.084057556763916	36.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	10.0
10	15.0
11	12.0
12	17.0
13	16.0
14	18.0
15	24.0
16	64.0
17	114.0
18	227.0
19	397.0
20	756.0
21	1181.0
22	1786.0
23	2754.0
24	4465.0
25	7382.0
26	12022.0
27	15077.0
28	14548.0
29	12193.0
30	10634.0
31	10498.0
32	11785.0
33	15565.0
34	24338.0
35	30600.0
36	36271.0
37	54457.0
38	99252.0
39	74337.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.741926922056095	15.179995326858991	17.931023531306643	28.147054219778276
2	42.61768488964808	25.81071056696997	18.830489749714737	12.741114793667213
3	25.968145511629544	23.326913435734102	38.64438077478864	12.060560277847724
4	23.138626686357377	20.025089776483213	42.87130487254348	13.964978664615929
5	23.11027024819823	23.377047618399473	37.730396060043056	15.78228607335924
6	18.51766152394304	32.53776510434035	38.1137751039547	10.830798267761905
7	59.11523375913361	6.347305117543107	30.649680025951813	3.8877810973714717
8	58.04109188166519	9.734424942776707	27.537957928119834	4.686525247438279
9	51.86392539307695	9.265976584387625	28.973701104993683	9.896396917541745
10	28.788136573680234	22.10236901026957	35.70665378150117	13.40284063454903
11	23.26362186576289	21.692902043251507	40.28519771242942	14.75827837855618
12	20.661635100279707	20.50034368003049	43.16825349294605	15.66976772674375
13	18.167402799801277	22.89793723926255	45.58467572711579	13.349984233820383
14	15.588101184845412	25.697511665838658	42.26493079894832	16.449456350367615
15	15.427717170617287	22.27250763922444	47.83753802598357	14.462237164174702
16	16.012994054222045	23.218251564708257	42.768767992160015	17.999986388909683
17	15.844897088814633	23.509075194468455	43.28825793923556	17.35776977748136
18	15.746897238536626	22.929015895484977	44.70721410471919	16.61687276125921
19	17.11095533974416	23.70825081609829	42.12836619277387	17.05242765138368
20	17.05877949353133	24.20596301866761	44.90820453839122	13.827052949409847
21	15.932688621355348	27.02504667469721	42.61292100803735	14.429343695910093
22	15.761188883368835	23.042668499626828	41.901060984490165	19.295081632514172
23	16.217387260473167	25.086600562138027	42.97452230744277	15.721489869946032
24	16.19969284306186	23.561477892186552	42.577078470204185	17.661750794547398
25	14.256709700397217	28.482567596077285	41.01248363833518	16.24823906519032
26	14.740130258134329	25.00629512927133	42.30599092140276	17.947583691191582
27	17.29606616804706	25.21681332616483	40.5719380150947	16.915182490693418
28	13.433692439266181	25.31345206741119	43.58452600512231	17.668329488200317
29	16.928793581009806	25.497201786682457	40.114832231969274	17.459172400338463
30	17.082145198574462	26.74261655063212	40.616400910128235	15.558837340665175
31	18.29806926683862	25.08387834407475	38.64392707177808	17.97412531730854
32	19.175304037729944	27.472397843095887	37.323424459583	16.028873659591167
33	16.256632570885422	27.015065208465188	36.73020777329368	19.998094447355705
34	16.199465991556586	28.17677176696906	37.90030783749266	17.723454403981698
35	19.02648945027075	28.2085309777073	34.18924406272898	18.575735509292972
36	17.284950444288675	29.778116542692317	33.51912471615206	19.417808296866955
37	19.51149796854477	28.25775775435158	35.138844463802435	17.09189981330121
38	16.659747695755836	31.06685994414916	32.54366324347745	19.729729116617552
39	19.43255364470971	27.15480573571346	35.95505617977528	17.45758443980155
40	17.36548272866065	30.678717018626777	35.658334410877984	16.297465841834594
41	15.848073009888457	29.133177713200713	34.38410950575862	20.6346397711522
42	17.09462203136449	28.534970293795386	35.715727841712095	18.654679833128032
43	19.033068143923668	26.15371004294299	33.94492499154978	20.86829682158356
44	17.910380044326786	27.086523432626237	34.681738680677014	20.321357842369963
45	17.400871563483257	26.87033394810091	34.94239106023588	20.78640342817995
46	19.073674563367565	28.84212723193525	34.20376255906646	17.880435645630726
47	16.350549094068516	25.961793669481892	39.87550389390609	17.812153342543503
48	17.245024579360596	27.477842279222443	35.93917657440616	19.3379565670108
49	17.382496591556134	25.450470376596186	38.43545053843205	18.731582493415637
50	17.017038816561065	25.305739116231905	37.209998706946415	20.467223360260604
51	17.583940728238705	24.476823715963768	34.8466597250106	23.092575830786924
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	535.0
1	1385.0
2	2235.0
3	16505.5
4	30776.0
5	20260.5
6	9745.0
7	9103.0
8	8461.0
9	8057.5
10	7654.0
11	7457.5
12	7261.0
13	6963.0
14	6665.0
15	6194.0
16	5723.0
17	5156.5
18	4590.0
19	4295.0
20	4000.0
21	3950.5
22	3901.0
23	3991.5
24	4082.0
25	4487.0
26	5525.5
27	6159.0
28	7479.5
29	8800.0
30	9202.5
31	9605.0
32	10908.5
33	12212.0
34	12965.0
35	13718.0
36	14338.0
37	14958.0
38	16085.5
39	17213.0
40	19120.5
41	21028.0
42	22887.0
43	24746.0
44	26091.0
45	27436.0
46	40119.5
47	52803.0
48	43228.0
49	33653.0
50	32185.0
51	30717.0
52	25798.5
53	20880.0
54	17634.0
55	14388.0
56	11929.0
57	9470.0
58	8061.0
59	6652.0
60	5677.0
61	4702.0
62	4003.0
63	3304.0
64	2882.0
65	2460.0
66	2083.5
67	1707.0
68	1454.5
69	1202.0
70	1074.0
71	946.0
72	797.5
73	649.0
74	508.5
75	314.0
76	260.0
77	195.5
78	131.0
79	96.0
80	61.0
81	48.0
82	35.0
83	26.5
84	18.0
85	11.5
86	5.0
87	7.5
88	10.0
89	5.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	440817.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.10932884207761
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.84341936903388	31.62356634506411
2	8.870838207501693	7.116067335391032
3	3.3065871218223615	3.97874970642456
4	1.8128846396079097	2.9085434465114
5	1.1068108083578316	2.2196719339195004
6	0.7861781627615354	1.8919847075197698
7	0.6040674238319125	1.6960117261682592
8	0.4612139492501101	1.4799185565620767
9	0.3804708680693324	1.373438804600122
>10	3.672921240351492	28.55507724332222
>50	0.10249951661018665	2.7257857628214843
>100	0.041228855843203564	3.189040724366052
>500	0.0051536069804004455	1.3295899714335668
>1k	0.0040083609847559026	3.0096643609717497
>5k	0.0011452459956445436	2.6619360457617964
>10k+	5.726229978222718E-4	4.240953329162344
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18465	4.18881304486896	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCG	6242	1.416007095915085	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTC	5348	1.213201850200877	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGC	4478	1.015841040613225	No Hit
GCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	2011	0.4561983771043313	TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCC	1679	0.38088367735364104	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCT	1451	0.32916153415135985	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCGATACTCGTA	1241	0.2815227180439956	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCT	1165	0.2642820036432352	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTC	1079	0.24477277418974314	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	844	0.19146267045054977	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	777	0.17626361959724784	No Hit
CTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGCT	692	0.15698124164902896	TruSeq Adapter, Index 20 (95% over 22bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	645	0.1463192209011903	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGC	627	0.14223589380627336	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	586	0.13293498209007365	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	564	0.12794424897406406	No Hit
CCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	550	0.12476832790023978	TruSeq Adapter, Index 23 (95% over 21bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	504	0.11433315865767427	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	462	0.1048053954362014	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCGATACT	450	0.10208317737292345	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16424048981777017	0.0
2	0.0	0.0	0.0	0.9099013876506578	0.0
3	0.0	0.0	0.0	1.1344843778710894	0.0
4	0.0	0.0	0.0	1.6809696540741397	0.0
5	0.0	0.0	0.0	3.619869469643866	0.0
6	0.0	0.0	0.0	4.191081559921691	0.0
7	0.0	0.0	0.0	5.007520127399806	0.0
8	0.0	0.0	0.0	5.957120528473267	0.0
9	0.0	0.0	0.0	6.276300596392607	0.0
10	0.0	0.0	0.0	8.485607406247944	0.0
11	0.0	0.0	0.0	9.742364745461268	0.0
12	0.0	0.0	0.0	11.74115335842311	0.0
13	0.0	0.0	0.0	12.188277675316515	0.0
14	0.0	0.0	0.0	12.404694011347113	0.0
15	0.0	0.0	0.0	12.965244080877099	0.0
16	0.0	0.0	0.0	13.555965400608416	0.0
17	0.0	0.0	0.0	14.214288468911136	0.0
18	0.0	0.0	0.0	14.925467937942502	0.0
19	0.0	0.0	0.0	15.443143072975861	0.0
20	0.0	0.0	0.0	15.865767427299764	0.0
21	0.0	0.0	0.0	16.3371648552574	0.0
22	0.0	0.0	0.0	16.82217337353142	0.0
23	0.0	0.0	0.0	17.32646426975366	0.0
24	0.0	0.0	0.0	17.723000700971152	0.0
25	0.0	0.0	0.0	18.081879782313294	0.0
26	0.0	0.0	0.0	18.428962585381235	0.0
27	0.0	0.0	0.0	18.794647211881575	0.0
28	0.0	0.0	0.0	19.164188313971557	0.0
29	0.0	0.0	0.0	19.53645163412482	0.0
30	0.0	0.0	0.0	19.96089080049091	0.0
31	0.0	0.0	0.0	20.337918002254902	0.0
32	0.0	0.0	0.0	20.73286647293548	0.0
33	0.0	0.0	0.0	21.1257732800686	0.0
34	0.0	0.0	0.0	21.508471769464425	0.0
35	0.0	0.0	0.0	21.88164249563878	0.0
36	0.0	0.0	0.0	22.2990492653414	0.0
37	0.0	0.0	0.0	22.695358845053615	0.0
38	0.0	0.0	0.0	23.09371008831329	0.0
39	0.0	0.0	0.0	23.485255786414772	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGCGC	30	2.1624455E-6	45.000004	15
TTGTGCG	20	7.0285506E-4	45.0	1
GTCGAAG	20	7.0285506E-4	45.0	1
CGACGGT	25	3.886879E-5	45.0	28
GCGAAAG	20	7.0285506E-4	45.0	1
CGACAGG	55	1.8189894E-12	45.0	2
GCCGATT	20	7.0285506E-4	45.0	9
GTTAACG	20	7.0285506E-4	45.0	1
GACGGTC	25	3.886879E-5	45.0	29
AACGAAG	20	7.0285506E-4	45.0	1
TTACGCG	20	7.0285506E-4	45.0	1
GCTCGAG	20	7.0285506E-4	45.0	1
TCGACGG	20	7.0285506E-4	45.0	2
GCGTACG	20	7.0285506E-4	45.0	1
ACGACCA	50	2.1827873E-11	45.0	28
GCTCACG	25	3.886879E-5	45.0	44
AACACGT	35	1.2096461E-7	45.0	41
CGTTTTT	7840	0.0	43.478954	1
CGAATAT	100	0.0	42.75	14
GCTACGA	100	0.0	42.75	10
>>END_MODULE