SRR3552190_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552190_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	410029
Sequences flagged as poor quality	0
Sequence length	51
%GC	33

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31486	7.678969048530713	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCG	10425	2.5425030912447655	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGC	8637	2.10643637401257	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTC	6494	1.5837904148243174	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCT	2237	0.545571166917462	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	2120	0.5170365998502545	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTA	1621	0.3953378907345578	No Hit
GAACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCT	1498	0.3653400125356987	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCC	1316	0.3209529082089316	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTC	1286	0.31363635255067324	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAGTCGAT	941	0.22949596248070261	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	754	0.1838894322108924	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	589	0.14364837609047165	No Hit
CTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGCT	560	0.13657570562082194	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGC	530	0.12925914996256363	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	501	0.12218647949291392	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	495	0.12072316836126225	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	494	0.12047928317265365	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	475	0.11584546458909004	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	475	0.11584546458909004	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATG	463	0.11291884232578671	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	437	0.10657782742196284	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	427	0.10413897553587673	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTT	420	0.10243177921561646	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGAGTCGATCGT	416	0.10145623846118201	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTAT	412	0.10048069770674757	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	20	7.0279714E-4	45.000004	4
CCGTCTC	40	6.7957444E-9	45.000004	19
ATCGTCG	20	7.0279714E-4	45.000004	2
GCGATAT	20	7.0279714E-4	45.000004	9
CGTGTAC	20	7.0279714E-4	45.000004	18
GCTACGA	45	3.8380676E-10	45.000004	10
GGTCTCG	20	7.0279714E-4	45.000004	9
CACCGAG	40	6.7957444E-9	45.000004	16
GTTACGG	25	3.886402E-5	45.0	2
GCGGGCC	25	3.886402E-5	45.0	5
GACTATG	35	1.2093733E-7	45.0	24
GTATGCG	30	2.1620726E-6	44.999996	1
CGTTTTT	12515	0.0	44.01119	1
TACGGCT	875	0.0	43.2	7
TGATACC	1315	0.0	43.11787	4
ACGGCTG	895	0.0	42.23464	8
GATACCT	1340	0.0	41.97761	5
GAATGAT	3250	0.0	41.746155	1
ATGATAC	3155	0.0	41.576862	3
TGCGGGT	65	0.0	41.53846	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content