##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552190_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	410029
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.840642978911248	31.0	30.0	33.0	28.0	34.0
2	30.79952149725995	31.0	30.0	33.0	26.0	34.0
3	30.58298559370191	31.0	30.0	34.0	25.0	34.0
4	34.58193444853901	35.0	35.0	37.0	32.0	37.0
5	35.265654380543815	35.0	35.0	37.0	33.0	37.0
6	35.22399635147758	36.0	35.0	37.0	33.0	37.0
7	35.67613754149097	37.0	35.0	37.0	35.0	37.0
8	35.10537303459024	37.0	35.0	37.0	32.0	37.0
9	37.17617290484331	39.0	37.0	39.0	33.0	39.0
10	36.951108336239635	39.0	37.0	39.0	33.0	39.0
11	37.195700791895206	39.0	37.0	39.0	34.0	39.0
12	37.38759209714435	39.0	37.0	39.0	34.0	39.0
13	37.22968375407593	39.0	37.0	39.0	34.0	39.0
14	38.483856019940056	40.0	38.0	41.0	34.0	41.0
15	38.48938489716581	40.0	38.0	41.0	33.0	41.0
16	38.45704816000819	40.0	38.0	41.0	34.0	41.0
17	38.075906826102546	40.0	37.0	41.0	33.0	41.0
18	37.654521997224585	39.0	37.0	40.0	33.0	41.0
19	36.890793090244834	37.0	37.0	40.0	33.0	41.0
20	36.16508832302105	36.0	35.0	40.0	32.0	41.0
21	36.418282609278855	36.0	35.0	40.0	33.0	41.0
22	36.6826346429155	37.0	35.0	40.0	33.0	41.0
23	36.73196286116348	37.0	35.0	40.0	33.0	41.0
24	36.5842318470157	37.0	35.0	40.0	33.0	41.0
25	36.19432284057957	36.0	35.0	40.0	33.0	41.0
26	36.30820015169659	37.0	35.0	40.0	33.0	41.0
27	36.52217769962613	37.0	35.0	40.0	33.0	41.0
28	36.333898334020276	37.0	35.0	40.0	33.0	41.0
29	36.2376636774472	37.0	35.0	40.0	33.0	41.0
30	35.76388743235235	36.0	35.0	40.0	31.0	41.0
31	35.43567406207853	37.0	35.0	40.0	30.0	41.0
32	34.71843942745513	37.0	35.0	40.0	22.0	41.0
33	33.64462025856708	36.0	33.0	40.0	16.0	41.0
34	32.86252435803321	37.0	33.0	40.0	10.0	41.0
35	32.356350404483585	37.0	32.0	40.0	9.0	41.0
36	31.911457482275644	37.0	30.0	40.0	8.0	41.0
37	31.5130320050533	36.0	28.0	40.0	7.0	41.0
38	31.298200858963636	36.0	26.0	40.0	7.0	41.0
39	31.189350021583838	36.0	25.0	40.0	7.0	41.0
40	30.987064329596198	35.0	24.0	40.0	7.0	41.0
41	30.830514427028334	35.0	23.0	40.0	7.0	41.0
42	30.616183245575314	35.0	23.0	40.0	7.0	41.0
43	30.576590924056593	35.0	23.0	40.0	7.0	41.0
44	30.54857583244112	35.0	23.0	40.0	7.0	41.0
45	30.55618505032571	35.0	23.0	40.0	7.0	41.0
46	30.349509424943115	35.0	23.0	40.0	7.0	41.0
47	30.244692448582907	35.0	23.0	40.0	7.0	41.0
48	30.253996668528323	35.0	23.0	40.0	7.0	41.0
49	30.224491438410453	35.0	23.0	40.0	7.0	41.0
50	30.174109636147687	35.0	23.0	40.0	7.0	41.0
51	28.764633721029487	34.0	20.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	9.0
10	13.0
11	11.0
12	9.0
13	7.0
14	18.0
15	25.0
16	47.0
17	160.0
18	289.0
19	567.0
20	897.0
21	1400.0
22	2102.0
23	3272.0
24	5639.0
25	10201.0
26	17306.0
27	21531.0
28	20703.0
29	17444.0
30	14775.0
31	13665.0
32	13637.0
33	16180.0
34	22897.0
35	30483.0
36	33615.0
37	40950.0
38	70271.0
39	51902.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.840147891978376	10.668513690495063	13.891212572769243	39.60012584475732
2	49.558201980835506	26.302773706250044	14.373373590648466	9.765650722265987
3	19.38594587212124	23.407124861900012	47.96855832148458	9.23837094449417
4	17.776791397681627	14.410200254128366	56.98987144811708	10.823136900072921
5	22.573037516858562	17.98872762658251	46.83156557219124	12.606669284367692
6	14.450929080626004	29.101356245533854	47.94953527677311	8.498179397067036
7	44.82439046994237	5.4098612537162	46.466469444844144	3.299278831497284
8	43.208163325033105	12.075975113955355	40.54713203212456	4.168729528886981
9	38.96187830616859	7.076328747478837	40.93198285974894	13.02981008660363
10	24.530216155442663	16.526879806062496	46.93131461433216	12.01158942416268
11	20.717802887112864	16.458591953252085	50.403020274175724	12.420584885459322
12	17.92190308490375	14.956503076611655	52.813581478383234	14.308012360101358
13	15.000890180938423	17.092205673257258	57.108887420158084	10.798016725646235
14	13.451975348085135	21.15045521170454	50.43155484124293	14.966014598967389
15	11.784532313568064	17.257803716322503	59.21239717190735	11.745266798202078
16	12.704955015376962	18.569418260659614	50.975906582217355	17.749720141746074
17	12.760073068002505	18.44698789597809	54.49175546119909	14.301183574820318
18	12.08890102895161	19.22473776245095	54.04227505859341	14.644086150004023
19	13.373444317353163	19.755188047674675	52.061195671525674	14.81017196344649
20	13.158581466188975	19.807867248414137	56.36625702084487	10.66729426455202
21	13.079318779891178	23.433464462269743	52.17923610281224	11.307980655026839
22	11.380902326420813	18.417965558533663	52.85748081233279	17.343651302712733
23	12.706906096885831	22.270619882983887	52.76041450726656	12.262059512863724
24	13.80951103458536	18.461133236917387	50.344243943721054	17.3851117847762
25	11.108238685556387	25.236507661653203	50.24498267195735	13.410270980833063
26	11.534793880432847	18.875006401986198	53.54718812571794	16.043011591863017
27	14.687741598764967	20.54781491065266	50.07987239926932	14.684571091313053
28	10.385119101331856	20.206131761411996	55.17365844854876	14.235090688707382
29	15.733521287518688	17.735330915618164	51.96242217013919	14.568725626723964
30	14.236310114650427	22.241353660350853	51.31539476476055	12.206941460238179
31	15.832050903716565	20.556350892253963	47.90514817244634	15.706450031583133
32	18.773794048713626	22.30671489089796	46.767179882398565	12.15231117798985
33	13.478558833643476	21.804311402364224	46.110153184286965	18.606976579705336
34	16.086423155435348	22.739854985866852	45.52751146870099	15.646210389996806
35	20.23539798404503	22.197454326401303	41.210011974762764	16.357135714790903
36	15.168683190701145	28.948196347087645	41.90996246606947	13.973157996141735
37	20.530742947450058	25.245775298820327	40.739801331125356	13.483680422604255
38	15.83327032965961	28.112645690914544	40.46372329762041	15.590360681805434
39	19.50179133671033	23.590526523733686	39.576225096273674	17.331457043282306
40	18.927441717537054	23.505654478097888	42.72307568489058	14.843828119474475
41	15.384277697431155	29.130622468166884	37.22541576327528	18.259684071126674
42	17.959217518760866	25.107736282067854	41.96629994463807	14.966746254533216
43	17.84264039860595	25.966456031158774	38.86773862336567	17.323164946869614
44	17.37974631062681	24.147804179704362	40.77540856866222	17.697040941006613
45	16.130810259762114	23.23884408176007	40.72150994197971	19.908835716498103
46	18.99451014440442	26.25448443890554	39.4947674432784	15.256237973411638
47	14.818951830236399	22.433779074163045	47.37811227986313	15.369156815737423
48	16.76174124269259	24.765077592072757	40.94368934880216	17.529491816432497
49	15.265017840201548	21.895280577715234	45.555070495013766	17.284631087069453
50	16.05081591789849	21.717732160408165	42.774779344875604	19.456672576817738
51	16.60711803311473	20.60805455223899	40.938323874652774	21.846503539993513
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	491.0
1	1238.5
2	1986.0
3	26288.5
4	50591.0
5	32482.5
6	14374.0
7	13395.0
8	12416.0
9	11873.0
10	11330.0
11	10980.5
12	10631.0
13	9979.5
14	9328.0
15	8549.0
16	7770.0
17	7045.5
18	6321.0
19	5764.5
20	5208.0
21	4894.5
22	4581.0
23	4351.0
24	4121.0
25	4535.0
26	5248.0
27	5547.0
28	6364.5
29	7182.0
30	7689.0
31	8196.0
32	8734.0
33	9272.0
34	9688.0
35	10104.0
36	10322.5
37	10541.0
38	10977.5
39	11414.0
40	12549.5
41	13685.0
42	14678.5
43	15672.0
44	17713.0
45	19754.0
46	37224.0
47	54694.0
48	40106.0
49	25518.0
50	24309.5
51	23101.0
52	19303.0
53	15505.0
54	13171.5
55	10838.0
56	9007.0
57	7176.0
58	6247.0
59	5318.0
60	4667.0
61	4016.0
62	3479.5
63	2943.0
64	2453.5
65	1964.0
66	1594.5
67	1225.0
68	1046.5
69	868.0
70	713.0
71	558.0
72	463.0
73	368.0
74	292.0
75	168.5
76	121.0
77	95.0
78	69.0
79	47.0
80	25.0
81	21.0
82	17.0
83	13.0
84	9.0
85	8.0
86	7.0
87	5.0
88	3.0
89	2.5
90	2.0
91	1.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	410029.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.42198114746456
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.7393625226788	34.25123714795386
2	8.318508164254276	7.057751930380498
3	3.2065674175547296	4.0808682760674255
4	1.7563125816750382	2.980250369154931
5	1.113980000391687	2.3628619287634356
6	0.7791246324319692	1.9831206281132547
7	0.5938046748444582	1.7633259505069547
8	0.4823744958151281	1.6370625413989668
9	0.40082115116304945	1.530326458632953
>10	2.4548723354173214	17.9084101878199
>50	0.09685499236912344	2.8350586556213373
>100	0.04813751117746854	4.142487423821613
>500	0.0034798200851182074	0.9533847340564681
>1k	0.0034798200851182074	2.479538412857054
>5k	0.001159940028372736	3.722752106066688
>10k+	0.001159940028372736	10.31156324878468
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31486	7.678969048530713	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCG	10425	2.5425030912447655	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGC	8637	2.10643637401257	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTC	6494	1.5837904148243174	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCT	2237	0.545571166917462	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	2120	0.5170365998502545	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTA	1621	0.3953378907345578	No Hit
GAACTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCT	1498	0.3653400125356987	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCC	1316	0.3209529082089316	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTC	1286	0.31363635255067324	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAGTCGAT	941	0.22949596248070261	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	754	0.1838894322108924	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	589	0.14364837609047165	No Hit
CTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGCT	560	0.13657570562082194	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGC	530	0.12925914996256363	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	501	0.12218647949291392	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	495	0.12072316836126225	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	494	0.12047928317265365	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	475	0.11584546458909004	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	475	0.11584546458909004	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATG	463	0.11291884232578671	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	437	0.10657782742196284	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	427	0.10413897553587673	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTT	420	0.10243177921561646	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGAGTCGATCGT	416	0.10145623846118201	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTAT	412	0.10048069770674757	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.438851886086106E-4	0.0	0.0	0.15730594665255385	0.0
2	2.438851886086106E-4	0.0	0.0	0.9955393399003485	0.0
3	2.438851886086106E-4	0.0	0.0	1.166746742303593	0.0
4	2.438851886086106E-4	0.0	0.0	1.7752402878820766	0.0
5	2.438851886086106E-4	0.0	0.0	4.237749037263218	0.0
6	2.438851886086106E-4	0.0	0.0	4.646256728182641	0.0
7	2.438851886086106E-4	0.0	0.0	5.5700938226320575	0.0
8	2.438851886086106E-4	0.0	0.0	6.374914945040473	0.0
9	2.438851886086106E-4	0.0	0.0	6.597094351862917	0.0
10	2.438851886086106E-4	0.0	0.0	10.009779796063205	0.0
11	2.438851886086106E-4	0.0	0.0	11.049706240290321	0.0
12	2.438851886086106E-4	0.0	0.0	14.108514275819514	0.0
13	2.438851886086106E-4	0.0	0.0	14.519948589002242	0.0
14	2.438851886086106E-4	0.0	0.0	14.743591306956338	0.0
15	2.438851886086106E-4	0.0	0.0	15.372571208377945	0.0
16	2.438851886086106E-4	0.0	0.0	15.868633682007857	0.0
17	2.438851886086106E-4	0.0	0.0	16.339088210833868	0.0
18	2.438851886086106E-4	0.0	0.0	16.830760751068826	0.0
19	2.438851886086106E-4	0.0	0.0	17.399257125715497	0.0
20	2.438851886086106E-4	0.0	0.0	17.776547512493018	0.0
21	2.438851886086106E-4	0.0	0.0	18.179201958885834	0.0
22	2.438851886086106E-4	0.0	0.0	18.62087803545603	0.0
23	2.438851886086106E-4	0.0	0.0	18.989876325820855	0.0
24	2.438851886086106E-4	0.0	0.0	19.306439300634832	0.0
25	2.438851886086106E-4	0.0	0.0	19.59129720092969	0.0
26	2.438851886086106E-4	0.0	0.0	19.860790334342205	0.0
27	2.438851886086106E-4	0.0	0.0	20.15540364218141	0.0
28	2.438851886086106E-4	0.0	0.0	20.447090327757305	0.0
29	2.438851886086106E-4	0.0	0.0	20.752678469083893	0.0
30	2.438851886086106E-4	0.0	0.0	21.068509788332044	0.0
31	2.438851886086106E-4	0.0	0.0	21.387999385409326	0.0
32	2.438851886086106E-4	0.0	0.0	21.71358611220182	0.0
33	2.438851886086106E-4	0.0	0.0	22.02771023512971	0.0
34	2.438851886086106E-4	0.0	0.0	22.353540847110814	0.0
35	2.438851886086106E-4	0.0	0.0	22.667908855227314	0.0
36	2.438851886086106E-4	0.0	0.0	22.971058144667815	0.0
37	2.438851886086106E-4	0.0	0.0	23.278841252691883	0.0
38	2.438851886086106E-4	0.0	0.0	23.605403520238813	0.0
39	2.438851886086106E-4	0.0	0.0	23.980742825507463	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	20	7.0279714E-4	45.000004	4
CCGTCTC	40	6.7957444E-9	45.000004	19
ATCGTCG	20	7.0279714E-4	45.000004	2
GCGATAT	20	7.0279714E-4	45.000004	9
CGTGTAC	20	7.0279714E-4	45.000004	18
GCTACGA	45	3.8380676E-10	45.000004	10
GGTCTCG	20	7.0279714E-4	45.000004	9
CACCGAG	40	6.7957444E-9	45.000004	16
GTTACGG	25	3.886402E-5	45.0	2
GCGGGCC	25	3.886402E-5	45.0	5
GACTATG	35	1.2093733E-7	45.0	24
GTATGCG	30	2.1620726E-6	44.999996	1
CGTTTTT	12515	0.0	44.01119	1
TACGGCT	875	0.0	43.2	7
TGATACC	1315	0.0	43.11787	4
ACGGCTG	895	0.0	42.23464	8
GATACCT	1340	0.0	41.97761	5
GAATGAT	3250	0.0	41.746155	1
ATGATAC	3155	0.0	41.576862	3
TGCGGGT	65	0.0	41.53846	4
>>END_MODULE