##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3552189_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 443763 Sequences flagged as poor quality 0 Sequence length 51 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.92486304626569 30.0 28.0 33.0 25.0 33.0 2 28.602134472680238 31.0 28.0 33.0 16.0 34.0 3 28.4535844583708 31.0 26.0 33.0 16.0 34.0 4 33.441521713166715 35.0 32.0 35.0 28.0 37.0 5 34.37631573610238 35.0 35.0 35.0 32.0 37.0 6 33.5790703596289 35.0 33.0 37.0 28.0 37.0 7 34.65595148761839 35.0 35.0 37.0 32.0 37.0 8 33.907385248432156 35.0 35.0 37.0 28.0 37.0 9 36.18957641804296 39.0 35.0 39.0 31.0 39.0 10 36.00601897859894 37.0 35.0 39.0 31.0 39.0 11 36.52399591673934 38.0 35.0 39.0 32.0 39.0 12 36.59563100123264 39.0 35.0 39.0 32.0 39.0 13 36.5364575234979 39.0 35.0 39.0 32.0 39.0 14 37.38863762864412 39.0 36.0 41.0 32.0 41.0 15 37.63866748692433 39.0 36.0 41.0 32.0 41.0 16 37.34076072137605 39.0 36.0 41.0 32.0 41.0 17 37.055700903410155 39.0 36.0 40.0 31.0 41.0 18 36.88390199273035 39.0 36.0 40.0 32.0 41.0 19 36.18948177292834 37.0 35.0 40.0 30.0 41.0 20 35.81413276906817 37.0 35.0 39.0 30.0 41.0 21 36.19175100222416 37.0 35.0 40.0 31.0 41.0 22 36.60844414698837 38.0 35.0 40.0 32.0 41.0 23 36.77026250498577 38.0 35.0 40.0 32.0 41.0 24 36.70756462345892 38.0 35.0 40.0 32.0 41.0 25 36.07491160822331 37.0 35.0 40.0 31.0 41.0 26 36.246171943131806 38.0 35.0 40.0 31.0 41.0 27 36.39296651591052 38.0 35.0 40.0 32.0 41.0 28 36.24010789543067 38.0 35.0 40.0 31.0 41.0 29 36.28583275306864 38.0 35.0 40.0 31.0 41.0 30 35.670767954966955 38.0 35.0 40.0 30.0 41.0 31 35.622618379630566 38.0 35.0 40.0 30.0 41.0 32 34.97762995112256 38.0 35.0 40.0 24.0 41.0 33 34.48580435953426 38.0 34.0 40.0 19.0 41.0 34 33.940846803361254 38.0 34.0 40.0 15.0 41.0 35 33.59711602815016 38.0 33.0 40.0 12.0 41.0 36 33.27354466235355 38.0 33.0 40.0 10.0 41.0 37 33.20762208656422 38.0 33.0 40.0 10.0 41.0 38 33.16992628948335 38.0 33.0 40.0 10.0 41.0 39 32.96877161908496 38.0 33.0 40.0 10.0 41.0 40 32.86307556060329 38.0 32.0 40.0 10.0 41.0 41 32.78986981789829 38.0 31.0 40.0 10.0 41.0 42 32.76521025862904 38.0 32.0 40.0 10.0 41.0 43 32.73257797518045 38.0 33.0 40.0 9.0 41.0 44 32.83111480677749 38.0 33.0 40.0 10.0 41.0 45 32.845462555463165 38.0 33.0 40.0 10.0 41.0 46 32.71337403073262 38.0 32.0 40.0 10.0 41.0 47 32.552141571063835 37.0 31.0 40.0 10.0 41.0 48 32.60441271579649 37.0 32.0 40.0 10.0 41.0 49 32.65482701351848 37.0 32.0 40.0 10.0 41.0 50 32.5837800808089 37.0 32.0 40.0 10.0 41.0 51 31.83904246185464 36.0 30.0 40.0 8.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 7.0 10 21.0 11 13.0 12 9.0 13 9.0 14 29.0 15 45.0 16 66.0 17 146.0 18 337.0 19 729.0 20 1554.0 21 2770.0 22 3593.0 23 4544.0 24 6096.0 25 9535.0 26 14094.0 27 16259.0 28 15330.0 29 13218.0 30 12106.0 31 13241.0 32 15696.0 33 21502.0 34 27912.0 35 35375.0 36 45129.0 37 68591.0 38 87753.0 39 28051.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.05231846728997 13.4456906051203 16.22510213785286 29.276888789736866 2 42.43255972219405 27.625782230605072 17.189355579442182 12.752302467758692 3 24.28210553831662 25.886790922181437 37.45377600205515 12.37732753744679 4 21.82809292347492 17.750240556332997 46.80403729017516 13.617629230016922 5 26.29556767914405 21.7512501042223 37.13063053927434 14.82255167735931 6 18.663115221413232 32.844333574453024 38.075729612428255 10.416821591705483 7 56.150693050119095 5.155905291788635 35.031762449776124 3.661639208316151 8 55.15984883823122 12.272316529318578 27.981828137992576 4.586006494457627 9 49.54356266745988 7.212183079706961 29.098415145021107 14.145839107812053 10 31.00866904180835 20.0237514168599 35.77968420080088 13.18789534053087 11 26.335679180102893 19.11763711710981 40.57683943906996 13.969844263717345 12 20.83251645585594 18.196875359144407 42.90894013245809 18.06166805254156 13 20.071299319681902 20.29799690375268 46.33757208239533 13.293131694170086 14 16.129330295675846 26.18221888710866 41.8369715366085 15.85147928060699 15 14.744582130551667 20.487287132996666 48.48443876573757 16.2836919707141 16 15.753228637808919 21.648492551204136 42.344224281880194 20.254054529106753 17 16.1414989532701 23.431200888762696 44.10845428753636 16.318845870430838 18 16.102514179866283 21.79767127948928 43.96896541622443 18.130849124420017 19 16.319071215941843 22.52531193452361 42.31109849176249 18.844518357772053 20 19.521906963852327 21.69333630789408 45.832798137744696 12.951958590508896 21 16.531121341797313 27.248553845183128 42.37081505217875 13.849509760840808 22 16.015756158129452 20.293264648021577 45.385261952889266 18.3057172409597 23 18.438671092452502 23.64167359604113 43.156820194563316 14.762835116943052 24 16.656188100404947 23.49091744917895 42.49002282750026 17.362871622915836 25 14.69252731750957 28.478940335269055 41.44667311154828 15.381859235673096 26 15.048347879386068 22.002059657970584 45.67911249923946 17.27047996340389 27 19.214084995819842 23.44832714759906 40.76838312342399 16.569204733157115 28 13.16761424454044 24.830371166591174 45.101551954534294 16.900462634334094 29 17.07578144189579 21.262926381875012 45.19867586977734 16.462616306451867 30 17.261466142963698 25.797103408801547 42.21848148673955 14.722948961495213 31 17.8973911750191 21.867077696878738 39.5253772847218 20.710153843380365 32 20.192309859091452 25.545167127498235 39.32774927157063 14.934773741839678 33 19.534977003490603 23.3744138199895 37.463465859028354 19.627143317491544 34 17.05302154528431 24.315456673945327 38.99040704159653 19.641114739173837 35 20.136424172362275 24.5800123038649 37.71359937624362 17.5699641475292 36 17.181017795535002 26.664007589636814 38.12800977098136 18.02696484384683 37 18.38098264163529 28.142499487338963 37.0337319695423 16.44278590148345 38 18.747845133551017 26.5069417684665 34.242151779215476 20.503061318767 39 18.589652584825682 27.929547979439473 34.83233167253692 18.648467763197925 40 19.781504992529797 24.73144448726009 37.911903425927804 17.575147094282308 41 16.40109698194757 26.02244891980629 36.427777890450535 21.148676207795603 42 16.759170998934117 25.368496246870514 39.44763308342516 18.42469967077021 43 18.984908611128013 24.900678966024657 36.58642113019788 19.527991292649453 44 19.02952702230695 24.995549426157655 36.19499597758263 19.77992757395276 45 16.825422579169512 24.782372572747164 36.47307233816249 21.919132509920836 46 19.85564366565036 27.43198509114099 35.69923585337219 17.013135389836467 47 16.958151085151307 24.969184001370103 40.97975721274644 17.092907700732148 48 17.955530316858322 25.77569558525609 36.61165982743041 19.657114270455175 49 17.291437095927332 23.84854077514349 40.18428755889968 18.675734570029498 50 17.436559605014388 23.915693737422906 38.72630210269896 19.921444554863747 51 17.44129186074549 23.305683439133052 36.75790906407249 22.495115636048972 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 356.0 1 1113.5 2 1871.0 3 16691.0 4 31511.0 5 20651.5 6 9792.0 7 9434.0 8 9076.0 9 8683.0 10 8290.0 11 7913.5 12 7537.0 13 7114.0 14 6691.0 15 6205.0 16 5719.0 17 5333.5 18 4948.0 19 4470.5 20 3993.0 21 4012.0 22 4031.0 23 3925.5 24 3820.0 25 4249.5 26 4926.0 27 5173.0 28 6143.0 29 7113.0 30 8016.0 31 8919.0 32 10217.0 33 11515.0 34 12732.0 35 13949.0 36 14390.5 37 14832.0 38 15813.0 39 16794.0 40 18178.5 41 19563.0 42 20842.5 43 22122.0 44 23381.0 45 24640.0 46 40769.0 47 56898.0 48 44091.5 49 31285.0 50 29711.0 51 28137.0 52 24774.5 53 21412.0 54 18797.0 55 16182.0 56 14246.5 57 12311.0 58 10465.5 59 8620.0 60 7690.5 61 6761.0 62 5931.5 63 5102.0 64 4237.0 65 3372.0 66 2821.0 67 2270.0 68 1880.5 69 1491.0 70 1264.5 71 1038.0 72 894.5 73 751.0 74 659.0 75 461.5 76 356.0 77 252.0 78 148.0 79 108.5 80 69.0 81 49.5 82 30.0 83 23.5 84 17.0 85 12.5 86 8.0 87 5.0 88 2.0 89 2.0 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 443763.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.53263918654643 #Duplication Level Percentage of deduplicated Percentage of total 1 78.33111089409674 30.966355440576827 2 8.45283748396905 6.683259487125268 3 3.4110063576522838 4.04538250800251 4 1.975280778023026 3.1235224915882 5 1.2869570618678075 2.5438404587698966 6 0.8972254178560981 2.1281813227862085 7 0.6498257564302293 1.79825290141566 8 0.5322754567427468 1.6833802863412166 9 0.45228425211907936 1.6091991133902508 >10 3.867087993316254 27.922961651514967 >50 0.09760339899419225 2.504860366612158 >100 0.03846722195653459 2.8816334917096733 >500 0.0022965505645692295 0.5460940596292907 >1k 0.0034448258468538443 2.2281817886038016 >5k 0.0017224129234269222 5.253261433965059 >10k+ 5.741376411423074E-4 4.08163319796905 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17983 4.052388324398383 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCG 8589 1.9354925940197807 No Hit GAATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTC 7496 1.6891899504915913 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGC 7060 1.5909393076935212 No Hit GCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC 1892 0.42635370682098334 TruSeq Adapter, Index 13 (95% over 21bp) GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCAGCTTT 1839 0.4144103947377316 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTA 1637 0.3688906015147725 No Hit GAATGACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCT 1602 0.36100350862960634 No Hit GAACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCT 1596 0.35965143556357787 No Hit GAATGATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTC 1251 0.28190723426693975 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCC 711 0.16022065832437585 No Hit GAATGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTT 625 0.14084094437796751 No Hit AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 551 0.12416537656361616 No Hit GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGT 519 0.11695432021146422 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 456 0.1027575530181651 No Hit GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATG 455 0.10253220750716036 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.06197001552630571 0.0 2 0.0 0.0 0.0 0.6235310289501378 0.0 3 0.0 0.0 0.0 0.7427388042716495 0.0 4 0.0 0.0 0.0 1.2265556163988436 0.0 5 0.0 0.0 0.0 3.297255517021473 0.0 6 0.0 0.0 0.0 3.701976054786001 0.0 7 0.0 0.0 0.0 4.299141658948583 0.0 8 0.0 0.0 0.0 4.895405881067147 0.0 9 0.0 0.0 0.0 5.045260645885303 0.0 10 0.0 0.0 0.0 7.398318471796882 0.0 11 0.0 0.0 0.0 8.042130596737447 0.0 12 0.0 0.0 0.0 10.212883904246185 0.0 13 0.0 0.0 0.0 10.519353799212643 0.0 14 0.0 0.0 0.0 10.6692085640308 0.0 15 0.0 0.0 0.0 11.195164986715882 0.0 16 0.0 0.0 0.0 11.59132239506223 0.0 17 0.0 0.0 0.0 11.94826968449375 0.0 18 0.0 0.0 0.0 12.272767220340587 0.0 19 0.0 0.0 0.0 12.970211576900283 0.0 20 0.0 0.0 0.0 13.274428016756692 0.0 21 0.0 0.0 0.0 13.55340575938057 0.0 22 0.0 0.0 0.0 13.914409268010177 0.0 23 0.0 0.0 0.0 14.19834461187616 0.0 24 0.0 0.0 0.0 14.444647255404348 0.0 25 0.0 0.0 0.0 14.67111949396412 0.0 26 0.0 0.0 0.0 14.908633662563124 0.0 27 0.0 0.0 0.0 15.174541365548727 0.0 28 0.0 0.0 0.0 15.398985494509457 0.0 29 0.0 0.0 0.0 15.63266878942138 0.0 30 0.0 0.0 0.0 15.905336857737126 0.0 31 0.0 0.0 0.0 16.160202630683496 0.0 32 0.0 0.0 0.0 16.41101218443178 0.0 33 0.0 0.0 0.0 16.651455844673848 0.0 34 0.0 0.0 0.0 16.91736354765945 0.0 35 0.0 0.0 0.0 17.183045905134048 0.0 36 0.0 0.0 0.0 17.453009827317736 0.0 37 0.0 0.0 0.0 17.713509238039222 0.0 38 0.0 0.0 0.0 18.010514621543482 0.0 39 0.0 0.0 0.0 18.31563244344391 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAACAC 45 3.8380676E-10 45.000004 34 TCCGGCT 35 1.2096825E-7 45.0 7 TCACGAT 25 3.886922E-5 45.0 7 CCTTCGA 20 7.0286E-4 45.0 41 AGTCCGG 20 7.0286E-4 45.0 2 CGAACCC 40 6.7993824E-9 45.0 34 ACGCCGG 20 7.0286E-4 45.0 27 CCCTCGA 40 6.7993824E-9 45.0 41 TCCGAAG 20 7.0286E-4 45.0 14 ACCGCGA 20 7.0286E-4 45.0 30 CACGATC 25 3.886922E-5 45.0 8 ACGATAT 25 3.886922E-5 45.0 32 TACGCAG 20 7.0286E-4 45.0 1 ACGGCTA 25 3.886922E-5 45.0 30 GCGGCTA 25 3.886922E-5 45.0 24 CGGCTAT 25 3.886922E-5 45.0 31 CCGTTAG 20 7.0286E-4 45.0 1 CACCCGC 25 3.886922E-5 45.0 38 TACCGCG 35 1.2096825E-7 45.0 29 AAATGCG 20 7.0286E-4 45.0 1 >>END_MODULE