FastQCFastQC Report
Sat 18 Jun 2016
SRR3552185_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552185_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399019
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174654.376984554620206No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCG87792.200145857716049No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGC80642.0209563955601113No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTC77571.9440177034176318No Hit
GCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC24840.6225267468466413No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGATCAT24270.6082417128006435No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCT17930.4493520358679661No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGATCATCGTA17680.44308667005831803No Hit
GAACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCT13330.33406930497044No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTC13180.33031008548465113No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCC8470.21227059363087974No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATG5360.1343294429588566No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGAGGATCA4830.1210468674424025No Hit
CCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC4500.11277658457366693No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4290.10751367729356245No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGGATCATCGT4270.1070124480287906No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGC4170.10450630170493135No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTT4160.10425568707254543No Hit
CTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGCT4090.10250138464584393No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTAT4010.10049646758675651No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCCG207.027743E-445.00000436
CGCGCAA207.027743E-445.00000418
CACGACC207.027743E-445.00000427
TCGGCTA207.027743E-445.0000041
CTGCGAG207.027743E-445.0000041
CCGTATG207.027743E-445.00000430
TTGCGCA207.027743E-445.00000432
CTACCGT207.027743E-445.00000427
ACCGTAT207.027743E-445.00000429
CGCAATC207.027743E-445.00000420
TAAGACG207.027743E-445.0000041
CGATAGA207.027743E-445.00000410
CCCATCG551.8189894E-1245.00000440
TCGAACG207.027743E-445.0000041
TACCGTA207.027743E-445.00000428
CCATTAC207.027743E-445.0000046
CTAGATG253.8862127E-545.01
AACCGCA253.8862127E-545.021
CGGCGAT700.045.031
CGGCGAA750.045.031