##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552185_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	399019
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.949090143577124	30.0	28.0	33.0	25.0	33.0
2	28.634626421298233	31.0	28.0	33.0	16.0	34.0
3	28.36186998614101	31.0	26.0	33.0	16.0	34.0
4	33.43123761023911	35.0	32.0	35.0	28.0	37.0
5	34.376678804768694	35.0	35.0	35.0	32.0	37.0
6	33.49791112703906	35.0	33.0	37.0	28.0	37.0
7	34.63077948669111	35.0	35.0	37.0	32.0	37.0
8	33.806685897162794	35.0	35.0	37.0	28.0	37.0
9	36.15242883170977	39.0	35.0	39.0	31.0	39.0
10	36.010696232510234	37.0	35.0	39.0	32.0	39.0
11	36.580170367827094	38.0	35.0	39.0	32.0	39.0
12	36.60501880862816	39.0	35.0	39.0	32.0	39.0
13	36.56018635704064	39.0	35.0	39.0	32.0	39.0
14	37.34603364752054	39.0	36.0	40.0	32.0	41.0
15	37.64218495861099	39.0	36.0	41.0	32.0	41.0
16	37.27186424706593	39.0	36.0	41.0	32.0	41.0
17	37.06669607211687	39.0	36.0	40.0	32.0	41.0
18	36.835293557449646	39.0	36.0	39.0	32.0	41.0
19	36.14286537733792	37.0	35.0	40.0	30.0	41.0
20	35.87262511308985	37.0	35.0	39.0	31.0	41.0
21	36.297740207859775	37.0	35.0	40.0	32.0	41.0
22	36.705066676022945	38.0	35.0	40.0	32.0	41.0
23	36.88492778539368	38.0	35.0	40.0	33.0	41.0
24	36.81680320987221	38.0	35.0	40.0	33.0	41.0
25	36.14311348582398	37.0	35.0	40.0	31.0	41.0
26	36.388427618734944	38.0	35.0	40.0	32.0	41.0
27	36.536220079745576	38.0	35.0	40.0	32.0	41.0
28	36.38668334089354	38.0	35.0	40.0	31.0	41.0
29	36.33825958162393	38.0	35.0	40.0	31.0	41.0
30	35.76559261589047	38.0	35.0	40.0	30.0	41.0
31	35.75045298594804	38.0	35.0	40.0	30.0	41.0
32	35.18220185003722	38.0	35.0	40.0	25.0	41.0
33	34.61448452329338	38.0	34.0	40.0	21.0	41.0
34	34.202827434282575	38.0	34.0	40.0	16.0	41.0
35	33.79774396707926	38.0	34.0	40.0	14.0	41.0
36	33.61502083860668	38.0	33.0	40.0	12.0	41.0
37	33.481711397201636	38.0	33.0	40.0	10.0	41.0
38	33.30034660003659	38.0	33.0	40.0	10.0	41.0
39	33.2285856061992	38.0	33.0	40.0	10.0	41.0
40	33.04675466581792	38.0	33.0	40.0	10.0	41.0
41	32.90924241702776	38.0	32.0	40.0	10.0	41.0
42	33.073397507386865	38.0	33.0	40.0	10.0	41.0
43	33.03772502061305	38.0	33.0	40.0	10.0	41.0
44	33.10262669196204	38.0	33.0	40.0	10.0	41.0
45	33.13802600878655	38.0	33.0	40.0	10.0	41.0
46	32.94618551998777	38.0	33.0	40.0	10.0	41.0
47	32.7572872469732	37.0	32.0	40.0	10.0	41.0
48	32.814720602277085	37.0	32.0	40.0	10.0	41.0
49	32.83671203626895	37.0	32.0	40.0	10.0	41.0
50	32.795032316756846	37.0	32.0	40.0	10.0	41.0
51	31.953027299451907	36.0	30.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	12.0
10	11.0
11	13.0
12	22.0
13	8.0
14	18.0
15	23.0
16	67.0
17	110.0
18	314.0
19	679.0
20	1255.0
21	1903.0
22	2511.0
23	3481.0
24	5229.0
25	8380.0
26	12180.0
27	14396.0
28	13308.0
29	11375.0
30	10883.0
31	11950.0
32	14253.0
33	19747.0
34	25508.0
35	32740.0
36	42555.0
37	63898.0
38	78323.0
39	23863.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.70453036070964	13.639701367604049	16.40999551399808	28.245772757688233
2	42.98291560051025	27.54630731869911	17.017735997533954	12.453041083256688
3	23.452517298674998	26.87215395758097	37.637806720983214	12.037522022760822
4	21.673153408734922	17.891378606031292	47.419295823005925	13.016172162227862
5	26.877918094125842	21.492209644152283	36.44112185134041	15.188750410381461
6	17.612444520185754	34.10363917507688	37.75359068114551	10.530325623591859
7	55.39335219626133	5.452873171452988	35.68050644204913	3.473268190236555
8	54.05030838130514	12.884348865592868	28.14226891451284	4.92307383858914
9	48.80168613524669	7.454031011054611	28.80639769033555	14.937885163363148
10	29.877023399888223	21.157388495284685	35.85769098714597	13.107897117681114
11	25.721080951032405	19.937897694094765	39.64698422882119	14.694037126051644
12	20.251416599209563	17.995383678471452	43.28440500327052	18.468794719048468
13	19.9426593721101	20.109568717279128	47.35589032101228	12.591881589598492
14	15.436107052546369	27.02953994671933	41.247409271237714	16.28694372949659
15	13.453494695741306	21.070174603214383	49.70690618742466	15.76942451361965
16	14.712081379583427	22.106716722762577	42.121302494367434	21.05989940328656
17	14.811826003273026	22.92948456088557	44.57431851616088	17.684370919680518
18	14.670980579872136	21.395973625316085	44.91440257230859	19.01864322250319
19	15.581714153962592	22.683380991882593	42.3801874096221	19.354717444532714
20	17.721963114538404	22.176888819830634	46.65341750643453	13.44773055919643
21	16.23832449081372	26.703490309985238	42.995446332129546	14.062738867071491
22	14.537402980810437	20.92832672128395	44.894854630982486	19.639415666923128
23	16.240580022505196	25.04767943381142	43.08491575589132	15.62682478779206
24	16.528786849749007	22.58514005598731	42.040604582739164	18.845468511524512
25	13.875780351311592	29.10838832236059	41.00757106804438	16.008260258283443
26	14.043441540377778	22.44404401795403	45.36225092038224	18.150263521285954
27	17.534753984146118	25.10381711146587	40.588292788062724	16.773136116325286
28	13.93993769720239	24.446956159982356	45.291326979417015	16.321779163398233
29	19.474260624180804	21.52428831709768	41.776957989469174	17.224493069252343
30	14.580007468316044	29.57778952881943	41.5681960006917	14.274007002172828
31	20.324094842601482	22.423744232730773	39.025459940504085	18.22670098416366
32	21.601978853137318	25.551665459539517	37.50723649751014	15.339119189813017
33	16.924507354286387	26.476684067675976	36.45239950979778	20.14640906823986
34	20.62382994293505	23.988581997348497	38.32799941857405	17.0595886411424
35	16.94631082730396	27.079161643931744	34.91563058400728	21.058896944757016
36	21.725782481535965	29.304118350254	33.26282708342209	15.707272084787943
37	19.235926108781786	28.453532287936163	36.139131219315374	16.171410383966677
38	19.671494339868527	28.818928421954844	32.58942556620111	18.920151671975518
39	21.517271107390876	26.12256559211466	36.47395236818297	15.886210932311496
40	17.30769712720447	26.889696981847983	36.145145970492635	19.657459920454915
41	16.37866868494984	29.02042258639313	35.47124322400688	19.129665504650152
42	17.02274829018167	25.90027041318834	36.6443703182054	20.432610978424588
43	19.987268776674792	27.695423024968736	33.1345123916405	19.182795806715973
44	18.663021059147557	25.971946198050723	35.528383360190865	19.83664938261085
45	17.549289632824504	24.91059322989632	35.26022570353793	22.27989143374125
46	21.666888042925276	27.778627082920863	33.57509291537495	16.979391958778905
47	15.649380104706792	26.417037785168123	41.22059350557242	16.712988604552663
48	18.454760299634852	26.321052380964318	35.65970542756109	19.564481891839737
49	16.9919226903982	23.631706760830937	40.26048884890193	19.11588169986893
50	18.30188537387944	24.29182570253547	37.12429733922445	20.281991584360647
51	17.865565298895543	23.20090020775953	34.96174367636628	23.97179081697864
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	336.0
1	957.0
2	1578.0
3	14882.5
4	28187.0
5	18372.0
6	8557.0
7	8099.0
8	7641.0
9	7479.0
10	7317.0
11	6861.0
12	6405.0
13	6176.5
14	5948.0
15	5364.0
16	4780.0
17	4485.5
18	4191.0
19	3956.5
20	3722.0
21	3561.0
22	3400.0
23	3600.5
24	3801.0
25	3913.0
26	4706.0
27	5387.0
28	6320.0
29	7253.0
30	8036.0
31	8819.0
32	9266.0
33	9713.0
34	10150.0
35	10587.0
36	11267.5
37	11948.0
38	13353.5
39	14759.0
40	15669.5
41	16580.0
42	18442.5
43	20305.0
44	21818.5
45	23332.0
46	39980.0
47	56628.0
48	43720.0
49	30812.0
50	29565.5
51	28319.0
52	24129.0
53	19939.0
54	17222.5
55	14506.0
56	12091.5
57	9677.0
58	8094.0
59	6511.0
60	5719.0
61	4927.0
62	4126.5
63	3326.0
64	2703.5
65	2081.0
66	1679.5
67	1278.0
68	1071.5
69	865.0
70	704.5
71	544.0
72	484.0
73	424.0
74	355.5
75	222.0
76	157.0
77	119.5
78	82.0
79	60.0
80	38.0
81	29.5
82	21.0
83	15.0
84	9.0
85	8.5
86	8.0
87	7.5
88	7.0
89	4.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	399019.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.30778635926022
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.42403533903541	31.219830128971378
2	8.73703221195227	6.868667912027891
3	3.123001609687913	3.6827484021971473
4	1.6654719861076028	2.618640680690018
5	1.0944403851209614	2.1510014420640613
6	0.768593297686212	1.8127020685565345
7	0.5845574820413084	1.6084362433150792
8	0.4674618133692388	1.469991127282433
9	0.34973121009335756	1.2372443720563724
>10	3.6291721876691754	27.7187061091613
>50	0.10968001478464816	2.7345103846010823
>100	0.03912561930914349	3.118995755845472
>500	0.0012828071904637211	0.34868411044655145
>1k	0.003848421571391163	2.8043480553123583
>5k	0.0019242107856955815	6.202190250893106
>10k+	6.414035952318606E-4	4.403302956579191
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17465	4.376984554620206	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCG	8779	2.200145857716049	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGC	8064	2.0209563955601113	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTC	7757	1.9440177034176318	No Hit
GCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	2484	0.6225267468466413	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGGATCAT	2427	0.6082417128006435	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCT	1793	0.4493520358679661	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGGATCATCGTA	1768	0.44308667005831803	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCT	1333	0.33406930497044	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTC	1318	0.33031008548465113	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCC	847	0.21227059363087974	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATG	536	0.1343294429588566	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGAGGATCA	483	0.1210468674424025	No Hit
CCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGC	450	0.11277658457366693	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	429	0.10751367729356245	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGGATCATCGT	427	0.1070124480287906	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGC	417	0.10450630170493135	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTT	416	0.10425568707254543	No Hit
CTGTCTCTTATACACATCTGACGCGAGGATCATCGTATGCCGTCTTCTGCT	409	0.10250138464584393	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAGGATCATCGTAT	401	0.10049646758675651	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1060099894992469	0.0
2	0.0	0.0	0.0	0.9473233104188021	0.0
3	0.0	0.0	0.0	1.0981933191151299	0.0
4	0.0	0.0	0.0	1.6310000275676095	0.0
5	0.0	0.0	0.0	4.131382214881998	0.0
6	0.0	0.0	0.0	4.580734250749964	0.0
7	0.0	0.0	0.0	5.359644528205424	0.0
8	0.0	0.0	0.0	6.1322894398512355	0.0
9	0.0	0.0	0.0	6.345311877379273	0.0
10	0.0	0.0	0.0	9.193296559812941	0.0
11	0.0	0.0	0.0	10.145632162879462	0.0
12	0.0	0.0	0.0	13.037725020613054	0.0
13	0.0	0.0	0.0	13.474045095596951	0.0
14	0.0	0.0	0.0	13.699598264744285	0.0
15	0.0	0.0	0.0	14.394302025718073	0.0
16	0.0	0.0	0.0	14.953423270571076	0.0
17	0.0	0.0	0.0	15.523320944616673	0.0
18	0.0	0.0	0.0	16.144594618301383	0.0
19	0.0	0.0	0.0	17.16860600623028	0.0
20	0.0	0.0	0.0	17.643771349233997	0.0
21	0.0	0.0	0.0	18.066057004804282	0.0
22	0.0	0.0	0.0	18.56452950861989	0.0
23	2.5061463238592647E-4	0.0	0.0	19.026662890739537	0.0
24	2.5061463238592647E-4	0.0	0.0	19.401081151524114	0.0
25	2.5061463238592647E-4	0.0	0.0	19.720364193183784	0.0
26	2.5061463238592647E-4	0.0	0.0	20.05267919572752	0.0
27	2.5061463238592647E-4	0.0	0.0	20.39927923231726	0.0
28	2.5061463238592647E-4	0.0	0.0	20.73384976655247	0.0
29	2.5061463238592647E-4	0.0	0.0	21.061904320345647	0.0
30	2.5061463238592647E-4	0.0	0.0	21.434818893335905	0.0
31	2.5061463238592647E-4	0.0	0.0	21.813497602871042	0.0
32	2.5061463238592647E-4	0.0	0.0	22.180648039316424	0.0
33	2.5061463238592647E-4	0.0	0.0	22.524491314949916	0.0
34	2.5061463238592647E-4	0.0	0.0	22.904172483014595	0.0
35	2.5061463238592647E-4	0.0	0.0	23.277337670637237	0.0
36	2.5061463238592647E-4	0.0	0.0	23.636719053478657	0.0
37	2.5061463238592647E-4	0.0	0.0	24.013894075219476	0.0
38	2.5061463238592647E-4	0.0	0.0	24.377535906811456	0.0
39	2.5061463238592647E-4	0.0	0.0	24.76047506509715	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCCCG	20	7.027743E-4	45.000004	36
CGCGCAA	20	7.027743E-4	45.000004	18
CACGACC	20	7.027743E-4	45.000004	27
TCGGCTA	20	7.027743E-4	45.000004	1
CTGCGAG	20	7.027743E-4	45.000004	1
CCGTATG	20	7.027743E-4	45.000004	30
TTGCGCA	20	7.027743E-4	45.000004	32
CTACCGT	20	7.027743E-4	45.000004	27
ACCGTAT	20	7.027743E-4	45.000004	29
CGCAATC	20	7.027743E-4	45.000004	20
TAAGACG	20	7.027743E-4	45.000004	1
CGATAGA	20	7.027743E-4	45.000004	10
CCCATCG	55	1.8189894E-12	45.000004	40
TCGAACG	20	7.027743E-4	45.000004	1
TACCGTA	20	7.027743E-4	45.000004	28
CCATTAC	20	7.027743E-4	45.000004	6
CTAGATG	25	3.8862127E-5	45.0	1
AACCGCA	25	3.8862127E-5	45.0	21
CGGCGAT	70	0.0	45.0	31
CGGCGAA	75	0.0	45.0	31
>>END_MODULE