SRR3552182_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552182_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	389261
Sequences flagged as poor quality	0
Sequence length	51
%GC	34

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25628	6.583757427535766	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCG	9624	2.4723771454114334	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTC	8257	2.121198887122008	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGC	7435	1.9100295174702835	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	2627	0.6748685329380545	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCT	2365	0.6075615075746092	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTC	1785	0.45856122241889113	No Hit
GAACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCT	1751	0.44982672294424564	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTA	1724	0.4428905027732036	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCC	1603	0.41180596052520035	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAGGGAAT	771	0.19806762043975637	No Hit
CTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCT	740	0.1901038120952266	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGC	676	0.1736624013194232	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	610	0.15670719645687597	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	553	0.1420640649846761	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTT	506	0.12998990394619547	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	479	0.12305368377515344	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGT	459	0.11791574290771487	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	459	0.11791574290771487	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	423	0.10866744934632548	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	410	0.10532778778249041	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTAT	401	0.10301571439214306	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	390	0.10018984691505185	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTCGTG	30	2.1617889E-6	45.000004	15
GGGTAAC	20	7.0275326E-4	45.0	7
CGACAGG	20	7.0275326E-4	45.0	2
GCACCGA	35	1.209155E-7	45.0	9
CGGATGG	35	1.209155E-7	45.0	2
GGGCCTA	20	7.0275326E-4	45.0	7
GAGCCGT	20	7.0275326E-4	45.0	33
CGTAAGG	25	3.886036E-5	45.0	2
TCGAGGA	20	7.0275326E-4	45.0	3
CGCACGG	20	7.0275326E-4	45.0	2
CGTTTTT	11705	0.0	43.712086	1
ACGGCTG	820	0.0	43.353657	8
CATGGGA	120	0.0	43.125004	4
TACGGCT	825	0.0	42.818184	7
CTACGAA	65	0.0	41.538464	11
CGAATAT	65	0.0	41.538464	14
GCTACGA	65	0.0	41.538464	10
AGCTACG	65	0.0	41.538464	9
CGGCTGT	845	0.0	41.27219	9
ACCCGTC	60	3.6379788E-12	41.250004	17

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content