##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552182_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	389261
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.75303459632483	31.0	30.0	33.0	28.0	34.0
2	30.72820035914207	31.0	30.0	33.0	26.0	34.0
3	30.399225712311278	31.0	30.0	34.0	25.0	34.0
4	34.52127235967641	35.0	35.0	37.0	32.0	37.0
5	35.219313005926615	35.0	35.0	37.0	33.0	37.0
6	35.187465479459796	36.0	35.0	37.0	33.0	37.0
7	35.65199185122579	37.0	35.0	37.0	35.0	37.0
8	35.07605436969026	37.0	35.0	37.0	32.0	37.0
9	37.14596890004393	39.0	37.0	39.0	33.0	39.0
10	36.88917461548935	39.0	37.0	39.0	32.0	39.0
11	37.15593393635633	39.0	37.0	39.0	34.0	39.0
12	37.36273091832986	39.0	37.0	39.0	34.0	39.0
13	37.2358443306676	39.0	37.0	39.0	34.0	39.0
14	38.45369045447656	40.0	38.0	41.0	34.0	41.0
15	38.50771076475681	40.0	38.0	41.0	34.0	41.0
16	38.41282841075782	40.0	38.0	41.0	34.0	41.0
17	38.06667505863675	40.0	37.0	41.0	33.0	41.0
18	37.68606667505863	39.0	37.0	40.0	33.0	41.0
19	36.91247774629362	37.0	36.0	40.0	33.0	41.0
20	36.30124261099879	36.0	35.0	40.0	32.0	41.0
21	36.53285327839162	37.0	35.0	40.0	33.0	41.0
22	36.82017720758052	38.0	35.0	40.0	33.0	41.0
23	36.87111732231074	38.0	35.0	40.0	33.0	41.0
24	36.745404754136686	38.0	35.0	40.0	33.0	41.0
25	36.31962102548162	37.0	35.0	40.0	33.0	41.0
26	36.46738563585872	37.0	35.0	40.0	33.0	41.0
27	36.66892650432486	38.0	35.0	40.0	33.0	41.0
28	36.48963805775559	38.0	35.0	40.0	33.0	41.0
29	36.400425421503826	38.0	35.0	40.0	33.0	41.0
30	35.946318793817	37.0	35.0	40.0	31.0	41.0
31	35.63141182908126	38.0	35.0	40.0	30.0	41.0
32	34.94697645024804	38.0	35.0	40.0	23.0	41.0
33	34.06242341256895	38.0	34.0	40.0	17.0	41.0
34	33.2562625076748	38.0	33.0	40.0	12.0	41.0
35	32.7195326528987	38.0	33.0	40.0	10.0	41.0
36	32.35100356829993	38.0	31.0	40.0	8.0	41.0
37	31.989074168745393	38.0	30.0	40.0	8.0	41.0
38	31.920053640102655	37.0	30.0	40.0	8.0	41.0
39	31.880452960866872	37.0	30.0	40.0	7.0	41.0
40	31.69432334603261	37.0	29.0	40.0	7.0	41.0
41	31.49340673738186	37.0	27.0	40.0	7.0	41.0
42	31.267758650365693	37.0	25.0	40.0	7.0	41.0
43	31.325830227019917	37.0	25.0	40.0	7.0	41.0
44	31.37584808136443	37.0	26.0	40.0	7.0	41.0
45	31.334372053712034	37.0	25.0	40.0	7.0	41.0
46	31.226357122855873	36.0	25.0	40.0	7.0	41.0
47	31.176652682904272	36.0	25.0	40.0	7.0	41.0
48	31.198689311284717	36.0	25.0	40.0	7.0	41.0
49	31.122221337354627	36.0	24.0	40.0	7.0	41.0
50	31.033620116066086	36.0	24.0	40.0	7.0	41.0
51	29.80440886705835	35.0	23.0	39.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	8.0
10	9.0
11	11.0
12	16.0
13	9.0
14	16.0
15	17.0
16	55.0
17	97.0
18	278.0
19	597.0
20	822.0
21	1277.0
22	1954.0
23	3159.0
24	5258.0
25	9445.0
26	15520.0
27	19446.0
28	18209.0
29	14719.0
30	11767.0
31	10702.0
32	11262.0
33	14041.0
34	21208.0
35	28284.0
36	32436.0
37	42095.0
38	74369.0
39	52168.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.76582036217345	11.803648451809968	13.61477260758206	37.81575857843452
2	47.692422307911656	26.336057298316558	15.174394557893034	10.797125835878754
3	19.975543401470993	24.627691959893234	45.96376210306195	9.433002535573818
4	18.259728048789885	15.494745170977827	54.836729084084965	11.408797696147314
5	22.626463992025915	18.579307970744566	45.03584998240255	13.758378054826967
6	15.246839524123917	29.289859503006983	46.55847875846797	8.904822214401134
7	47.593773843256834	4.924973218483228	43.90575988860944	3.575493049650492
8	45.341557464015146	11.654905063697623	38.4007131461924	4.602824326094831
9	41.5972830568693	6.975782315721328	38.52993235900848	12.897002268400893
10	24.678043780394134	18.53075442954727	44.97676366242701	11.81443812763159
11	20.605198054775585	17.12501380821608	48.792712344673625	13.477075792334706
12	17.827884118881677	15.986189214948327	51.50991237241851	14.676014293751493
13	15.221149819786724	18.39459899656015	55.04070533652228	11.343545847130846
14	13.454982646604721	23.003075057609163	48.249375097942	15.29256719784412
15	11.631784329794147	18.452657728362205	57.2030077505838	12.712550191259849
16	12.79681242148584	20.126598862973687	48.760600214252136	18.315988501288338
17	12.056435142487945	19.714536005405115	52.791314824757684	15.437714027349259
18	12.338764993153694	20.101422952723237	52.20173611021911	15.358075943903962
19	13.268475393116699	20.65888953684032	49.97289736192426	16.099737708118717
20	13.44830332347705	20.676872329876357	54.29801598413404	11.576808362512555
21	13.11793372570075	24.269834378476137	50.20795815660958	12.404273739213535
22	12.226244088156788	19.05328301576577	50.29530315135603	18.42516974472141
23	12.806574509133975	22.959659457279304	50.65752798251045	13.57623805107627
24	14.035569964625278	19.727637754617085	48.679420748546605	17.557371532211036
25	11.23487839778452	26.45294545305078	48.08547478427071	14.226701364893993
26	12.498554954131032	20.25658876691988	50.70659531779449	16.5382609611546
27	15.917083910281276	21.854488376693272	47.247990422878225	14.980437290147227
28	10.967705472677714	21.093302437182253	52.34611224859413	15.592879841545903
29	16.179375791564016	18.052155237745367	50.11470452986556	15.65376444082505
30	16.136987779407647	22.604113949252557	48.55662396181482	12.702274309524972
31	17.00504288896139	22.43250672428011	45.3374471113212	15.225003275437304
32	19.45686827090307	23.627334873002948	44.192713886055884	12.723082970038096
33	17.756980534911023	22.69222963512913	41.360167085837006	18.190622744122837
34	19.03863988429357	23.727267822874627	43.003794369330606	14.230297923501197
35	19.364385335289175	25.549181654468338	38.56512725395043	16.52130575629205
36	17.579721574984394	29.962929756641433	38.9507297160517	13.506618952322476
37	21.248468251378895	26.553649094052577	38.90962618911219	13.288256465456339
38	19.05739336845972	29.555491045853554	35.529374892424364	15.857740693262363
39	24.210491161457224	24.462764058048457	37.11751241454962	14.209232365944699
40	20.53583585306517	26.822106504376244	38.203673113926136	14.438384528632461
41	18.18137445056145	30.519625649628395	35.01429632046365	16.284703579346505
42	16.691114701960895	28.299007606721453	39.812876193607885	15.197001497709763
43	19.208191932919043	26.77971849221987	36.793308345814246	17.218781229046833
44	18.88938270209448	24.455570940834043	38.128402280218154	18.52664407685332
45	17.343633192125594	24.049930509349768	38.562301386473344	20.044134912051298
46	20.220109386761067	27.262171139672354	37.37004220818423	15.147677265382352
47	15.71773180462466	23.225291000125882	45.30482118681296	15.752156008436499
48	17.577409501594047	25.42407279434621	38.84694331052944	18.151574393530304
49	16.21482758354934	22.34310655318668	44.39514875623296	17.046917107031014
50	17.024567064257656	22.428910165672903	41.00693365120061	19.53958911886883
51	17.308181400140267	21.42110306452483	38.51349094823268	22.757224587102225
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	466.0
1	1045.0
2	1624.0
3	21384.0
4	41144.0
5	26924.0
6	12704.0
7	11998.5
8	11293.0
9	10777.0
10	10261.0
11	9870.0
12	9479.0
13	8883.0
14	8287.0
15	7457.5
16	6628.0
17	6009.0
18	5390.0
19	4968.0
20	4546.0
21	4247.5
22	3949.0
23	3895.5
24	3842.0
25	4148.0
26	4795.5
27	5137.0
28	5564.0
29	5991.0
30	6843.0
31	7695.0
32	8194.5
33	8694.0
34	9324.0
35	9954.0
36	10126.5
37	10299.0
38	11183.5
39	12068.0
40	13419.0
41	14770.0
42	16295.0
43	17820.0
44	18865.5
45	19911.0
46	37877.5
47	55844.0
48	40884.5
49	25925.0
50	24880.0
51	23835.0
52	19742.5
53	15650.0
54	12961.5
55	10273.0
56	8367.5
57	6462.0
58	5431.5
59	4401.0
60	3795.5
61	3190.0
62	2819.0
63	2448.0
64	2033.0
65	1618.0
66	1332.0
67	1046.0
68	900.5
69	755.0
70	651.5
71	548.0
72	448.5
73	349.0
74	286.0
75	191.0
76	159.0
77	115.0
78	71.0
79	50.5
80	30.0
81	21.0
82	12.0
83	10.0
84	8.0
85	6.0
86	4.0
87	2.5
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	389261.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.16914299918766
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.56857426229823	33.16939155045226
2	8.428540597062401	6.939915862298412
3	3.0952749259077557	3.8228944813948917
4	1.6717799286889095	2.753029877894619
5	1.0630467730769646	2.1882362307815275
6	0.7447101630344659	1.8395447516948569
7	0.6183641344381974	1.7820265033378535
8	0.4307593541011832	1.4187194757783461
9	0.39189831198258057	1.4520705882436007
>10	2.841116377092267	21.185703576463453
>50	0.09476309890716991	2.6843651998340086
>100	0.0410640095264403	3.4279559474675154
>500	0.0037905239562867968	1.0028981891832125
>1k	0.0037905239562867968	3.083339738788118
>5k	0.0018952619781433984	6.584380331266132
>10k+	6.317539927144661E-4	6.665527695121205
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25628	6.583757427535766	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCG	9624	2.4723771454114334	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTC	8257	2.121198887122008	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGC	7435	1.9100295174702835	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	2627	0.6748685329380545	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCT	2365	0.6075615075746092	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTC	1785	0.45856122241889113	No Hit
GAACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCT	1751	0.44982672294424564	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTA	1724	0.4428905027732036	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCC	1603	0.41180596052520035	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAGGGAAT	771	0.19806762043975637	No Hit
CTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCT	740	0.1901038120952266	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGC	676	0.1736624013194232	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	610	0.15670719645687597	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	553	0.1420640649846761	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTT	506	0.12998990394619547	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	479	0.12305368377515344	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGT	459	0.11791574290771487	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	459	0.11791574290771487	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	423	0.10866744934632548	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	410	0.10532778778249041	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTAT	401	0.10301571439214306	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	390	0.10018984691505185	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20731591400114577	0.0
2	0.0	0.0	0.0	1.1948281487228363	0.0
3	0.0	0.0	0.0	1.441963104446631	0.0
4	0.0	0.0	0.0	2.19210247109266	0.0
5	0.0	0.0	0.0	5.107627016320669	0.0
6	0.0	0.0	0.0	5.658157380266711	0.0
7	0.0	0.0	0.0	6.7152887137421935	0.0
8	0.0	0.0	0.0	7.790659737297084	0.0
9	0.0	0.0	0.0	8.070677514572484	0.0
10	0.0	0.0	0.0	11.326590642268298	0.0
11	0.0	0.0	0.0	12.521161893947763	0.0
12	0.0	0.0	0.0	15.41793295500962	0.0
13	0.0	0.0	0.0	15.870842442474329	0.0
14	0.0	0.0	0.0	16.11900498637161	0.0
15	0.0	0.0	0.0	16.822902885210695	0.0
16	0.0	0.0	0.0	17.39244363036626	0.0
17	0.0	0.0	0.0	17.956589537611013	0.0
18	0.0	0.0	0.0	18.535635473371336	0.0
19	0.0	0.0	0.0	19.114938306175034	0.0
20	0.0	0.0	0.0	19.505164915056994	0.0
21	0.0	0.0	0.0	19.898474288459415	0.0
22	0.0	0.0	0.0	20.35626481974819	0.0
23	0.0	0.0	0.0	20.76164835418909	0.0
24	0.0	0.0	0.0	21.096898995789456	0.0
25	0.0	0.0	0.0	21.40620303600926	0.0
26	0.0	0.0	0.0	21.702148429973718	0.0
27	0.0	0.0	0.0	22.02095766079828	0.0
28	0.0	0.0	0.0	22.338225509362612	0.0
29	0.0	0.0	0.0	22.640079535324627	0.0
30	0.0	0.0	0.0	23.022342335862056	0.0
31	0.0	0.0	0.0	23.34526191938057	0.0
32	0.0	0.0	0.0	23.670750473332802	0.0
33	0.0	0.0	0.0	23.989045910070622	0.0
34	0.0	0.0	0.0	24.295010288726587	0.0
35	0.0	0.0	0.0	24.629490239196837	0.0
36	0.0	0.0	0.0	24.929289088811878	0.0
37	0.0	0.0	0.0	25.25195177528702	0.0
38	0.0	0.0	0.0	25.55329200716229	0.0
39	0.0	0.0	0.0	25.88109263450487	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTCGTG	30	2.1617889E-6	45.000004	15
GGGTAAC	20	7.0275326E-4	45.0	7
CGACAGG	20	7.0275326E-4	45.0	2
GCACCGA	35	1.209155E-7	45.0	9
CGGATGG	35	1.209155E-7	45.0	2
GGGCCTA	20	7.0275326E-4	45.0	7
GAGCCGT	20	7.0275326E-4	45.0	33
CGTAAGG	25	3.886036E-5	45.0	2
TCGAGGA	20	7.0275326E-4	45.0	3
CGCACGG	20	7.0275326E-4	45.0	2
CGTTTTT	11705	0.0	43.712086	1
ACGGCTG	820	0.0	43.353657	8
CATGGGA	120	0.0	43.125004	4
TACGGCT	825	0.0	42.818184	7
CTACGAA	65	0.0	41.538464	11
CGAATAT	65	0.0	41.538464	14
GCTACGA	65	0.0	41.538464	10
AGCTACG	65	0.0	41.538464	9
CGGCTGT	845	0.0	41.27219	9
ACCCGTC	60	3.6379788E-12	41.250004	17
>>END_MODULE