##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552180_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	804155
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.288681908338567	31.0	30.0	33.0	27.0	33.0
2	30.258476288775174	31.0	30.0	33.0	25.0	34.0
3	29.923547077366926	31.0	30.0	33.0	25.0	34.0
4	34.3546629692037	35.0	33.0	37.0	32.0	37.0
5	34.976615204780174	35.0	35.0	37.0	33.0	37.0
6	34.92560389477153	35.0	35.0	37.0	32.0	37.0
7	35.40256791290236	36.0	35.0	37.0	33.0	37.0
8	34.71743631513825	36.0	35.0	37.0	32.0	37.0
9	36.5669118515709	39.0	35.0	39.0	32.0	39.0
10	36.39610647201099	37.0	35.0	39.0	32.0	39.0
11	36.76325957060517	39.0	35.0	39.0	32.0	39.0
12	37.027030858478774	39.0	37.0	39.0	33.0	39.0
13	36.80933526496757	39.0	37.0	39.0	32.0	39.0
14	37.99955481219417	40.0	37.0	41.0	33.0	41.0
15	37.95820084436458	40.0	37.0	41.0	33.0	41.0
16	37.84455981744813	39.0	37.0	41.0	33.0	41.0
17	37.491341843301356	39.0	36.0	41.0	32.0	41.0
18	37.29341731382632	39.0	36.0	40.0	32.0	41.0
19	36.62288116097021	37.0	36.0	40.0	32.0	41.0
20	36.25662465569449	37.0	35.0	40.0	31.0	41.0
21	36.64433598000385	38.0	35.0	40.0	32.0	41.0
22	37.02740765150997	38.0	35.0	40.0	33.0	41.0
23	37.07100123732365	38.0	35.0	40.0	33.0	41.0
24	36.96021165073898	38.0	35.0	40.0	33.0	41.0
25	36.52868787733708	38.0	35.0	40.0	32.0	41.0
26	36.711546903271135	38.0	35.0	40.0	32.0	41.0
27	36.95231516312154	38.0	35.0	40.0	33.0	41.0
28	36.724933625980064	38.0	35.0	40.0	32.0	41.0
29	36.677961338299205	38.0	35.0	40.0	32.0	41.0
30	36.37921669329918	38.0	35.0	40.0	31.0	41.0
31	36.298538217134755	39.0	35.0	40.0	31.0	41.0
32	35.791149716161684	38.0	35.0	40.0	29.0	41.0
33	35.20806063507657	38.0	35.0	40.0	23.0	41.0
34	34.59289813530974	38.0	35.0	40.0	18.0	41.0
35	34.37445392990158	38.0	34.0	40.0	17.0	41.0
36	34.205531271956275	38.0	34.0	40.0	15.0	41.0
37	33.75624724089261	38.0	33.0	40.0	12.0	41.0
38	33.83353333623493	38.0	33.0	40.0	13.0	41.0
39	33.499402478377924	38.0	33.0	40.0	12.0	41.0
40	33.38731960878189	38.0	33.0	40.0	12.0	41.0
41	33.08015370171174	38.0	32.0	40.0	10.0	41.0
42	33.06117726060275	38.0	32.0	40.0	10.0	41.0
43	33.2377986830897	38.0	33.0	40.0	10.0	41.0
44	33.28971280412358	38.0	33.0	40.0	10.0	41.0
45	33.250835970677294	38.0	33.0	40.0	10.0	41.0
46	33.0897414055748	38.0	33.0	40.0	10.0	41.0
47	32.95195080550391	37.0	32.0	40.0	10.0	41.0
48	33.002826569504634	38.0	32.0	40.0	10.0	41.0
49	33.038286151301676	38.0	32.0	40.0	10.0	41.0
50	32.97908363437397	38.0	32.0	40.0	10.0	41.0
51	31.46839353109786	35.0	28.0	39.0	9.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	11.0
10	21.0
11	17.0
12	18.0
13	19.0
14	34.0
15	38.0
16	81.0
17	174.0
18	383.0
19	774.0
20	1427.0
21	2229.0
22	3275.0
23	5180.0
24	8135.0
25	13885.0
26	22036.0
27	27239.0
28	26850.0
29	23789.0
30	22298.0
31	22950.0
32	26447.0
33	34022.0
34	48039.0
35	62825.0
36	80369.0
37	116745.0
38	166850.0
39	87981.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.07717417662018	12.056382165129857	12.004402136404051	27.862041521845914
2	37.56614085592951	34.77625582132798	14.484023602414958	13.173579720327549
3	20.005968998513968	33.523885320616046	35.22778568808252	11.24235999278746
4	18.627503404194464	15.630320025368244	51.73206657920426	14.010109991233033
5	27.717417662017894	18.27794392872021	35.90564008182502	18.098998327436874
6	15.140613438951444	33.7257120828696	39.57843947995101	11.555234998227954
7	50.90312191057694	4.542283514994	39.653673732054145	4.900920842374915
8	46.49650875764001	15.160385746528965	31.102959006659166	7.240146489171864
9	42.390086488301385	6.20415218459128	30.69507744153801	20.710683885569324
10	26.53058179082391	18.067163668695713	38.74302839626689	16.65922614421349
11	21.42509839520988	17.937959721695446	42.52687603757982	18.110065845514857
12	19.22092133979146	14.954082235389944	45.85695543769547	19.96804098712313
13	16.36550167567198	17.749190143691205	52.87189658710074	13.013411593536073
14	15.085648910968658	24.76736450062488	40.45650403218285	19.690482556223614
15	11.179063737712257	18.827589208548105	54.830225516225106	15.163121537514535
16	11.988857869440594	22.799709011322445	40.2918591565059	24.919573962731064
17	12.374480044270072	21.253862750340417	46.22864994932569	20.143007256063818
18	13.718748251269966	21.098917497248664	45.70561645453924	19.47671779694213
19	12.507041552934448	24.038524911242234	40.82819854381307	22.626234992010247
20	13.769484738638695	22.66814233574373	48.226896556012214	15.335476369605361
21	13.911870224023975	28.347271359377235	42.639167822123845	15.101690594474945
22	12.498709825842033	19.139966797445766	42.57338448433449	25.787938892377717
23	12.292530668838719	27.116289770007025	43.455055306501855	17.136124254652398
24	17.581187706350143	18.618425552287803	39.68874159832371	24.111645143038345
25	11.951800336999707	31.22805926718108	38.19052297131772	18.629617424501497
26	13.165496701506552	18.96425440369083	43.2092071802078	24.661041714594823
27	17.63739577569001	21.33593647990748	37.15925412389402	23.867413620508486
28	10.93620011067518	19.306725693429748	45.766052564493165	23.991021631401907
29	17.064247564213368	16.545317755905266	38.88989063053764	27.500544049343723
30	13.136397833750957	22.438584601227376	39.77181016097643	24.65320740404524
31	15.467043045184075	19.723933818728977	35.76462249193252	29.044400644154422
32	16.64181656521442	23.429935771088907	39.19107634722162	20.73717131647506
33	11.911882659437547	20.712051781062108	33.95626465047161	33.41980090902873
34	15.195080550391404	25.471084554594576	34.34474697042237	24.98908792459165
35	11.998930554432913	27.768278503522332	31.505742052216302	28.727048889828456
36	13.114014089323575	28.841579048815213	29.408012136963645	28.636394724897563
37	12.83322245089566	24.926537794330695	40.26052191430757	21.97971784046608
38	13.087526658417842	25.12687230695575	30.393643016582622	31.391958018043788
39	17.79147054983181	26.45534753872077	35.68727421952236	20.065907691925062
40	15.49241128886844	26.835995548121943	35.086146327511486	22.585446835498132
41	13.651970080394948	35.0878872854114	29.58272969763292	21.67741293656074
42	15.187370593977528	26.137995784394803	39.64857521249013	19.02605840913754
43	19.331347812299867	25.030995268325135	31.55647853958503	24.081178379789968
44	19.002306769217377	21.48118211041404	34.478427666308114	25.038083454060473
45	15.514795033295819	19.90188458692665	35.5323289664306	29.050991413346928
46	22.431745123763452	25.54432914052639	31.79797427112932	20.22595146458083
47	12.846901405823505	20.95690507427051	45.37085512121419	20.825338398691795
48	17.847305556764553	23.674291647754476	32.98331789269482	25.495084902786154
49	14.933688157133886	18.946347408148924	42.63978959280239	23.480174841914806
50	16.80745627397703	18.6118347830953	38.66941074792795	25.91129819499972
51	16.969987129346954	18.642301546343678	33.05992004029074	31.32779128401863
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	530.0
1	1443.0
2	2356.0
3	27372.0
4	52388.0
5	33630.5
6	14873.0
7	14190.5
8	13508.0
9	13016.5
10	12525.0
11	11975.0
12	11425.0
13	10681.5
14	9938.0
15	9367.0
16	8796.0
17	8314.0
18	7832.0
19	7257.5
20	6683.0
21	6428.5
22	6174.0
23	6347.0
24	6520.0
25	6428.0
26	6723.0
27	7110.0
28	7958.0
29	8806.0
30	9971.5
31	11137.0
32	12723.5
33	14310.0
34	14945.0
35	15580.0
36	16808.0
37	18036.0
38	19509.0
39	20982.0
40	22739.5
41	24497.0
42	27871.0
43	31245.0
44	36347.0
45	41449.0
46	110377.0
47	179305.0
48	125693.0
49	72081.0
50	70794.0
51	69507.0
52	56936.5
53	44366.0
54	36843.0
55	29320.0
56	24113.0
57	18906.0
58	15719.0
59	12532.0
60	10534.0
61	8536.0
62	7122.5
63	5709.0
64	4666.0
65	3623.0
66	2993.5
67	2364.0
68	1994.5
69	1625.0
70	1332.5
71	1040.0
72	944.5
73	849.0
74	706.5
75	456.0
76	348.0
77	256.0
78	164.0
79	150.5
80	137.0
81	105.0
82	73.0
83	52.5
84	32.0
85	25.5
86	19.0
87	12.5
88	6.0
89	5.5
90	5.0
91	5.0
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	804155.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.05130593781041
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.98366327685727	29.67380229498887
2	9.957250171016277	7.577727451133108
3	3.807664358053162	4.346598041903322
4	1.9391265923727659	2.951451968740796
5	1.1480551898241254	2.18424996307444
6	0.8073913757153328	1.8433377749336262
7	0.6259041174010874	1.6671528341274788
8	0.47445661408673495	1.44429550214656
9	0.41014243612671986	1.4045809783623016
>10	2.691208969554113	18.162057377954937
>50	0.09890391022776703	2.5834198954111187
>100	0.04829421388021531	3.3975273899976295
>500	0.0023154760079555003	0.550164569395011
>1k	0.002646258294806286	2.0443314884726083
>5k	0.0016539114342539287	4.554748403728498
>10k+	0.001323129147403143	15.614554065629719
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG	36658	4.558573906771705	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC	33940	4.220579365918263	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29980	3.7281369885158955	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGC	23478	2.9195863981446366	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	9395	1.1683071049735436	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCT	8211	1.0210718082956645	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTC	6383	0.7937524482220468	No Hit
GAACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCT	6363	0.7912653655078934	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTA	5835	0.7256063818542445	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTT	4578	0.5692932332697054	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCC	2203	0.2739521609639933	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCTCAAAT	1981	0.24634554283689092	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	1882	0.23403448340183175	No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT	1662	0.20667657354614474	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATG	1442	0.17931866369045768	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGT	1314	0.16340133431987613	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTAT	1180	0.14673788013504857	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.487082714153366E-4	0.0	0.0	0.23005515105918636	0.0
2	2.487082714153366E-4	0.0	0.0	1.9460178696893011	0.0
3	2.487082714153366E-4	0.0	0.0	2.3608632664100826	0.0
4	2.487082714153366E-4	0.0	0.0	3.6384776566706667	0.0
5	2.487082714153366E-4	0.0	0.0	9.310767202840248	0.0
6	2.487082714153366E-4	0.0	0.0	10.772923130491012	0.0
7	2.487082714153366E-4	0.0	0.0	12.626545877349516	0.0
8	2.487082714153366E-4	0.0	0.0	14.58251207789543	0.0
9	2.487082714153366E-4	0.0	0.0	15.048467024392064	0.0
10	2.487082714153366E-4	0.0	0.0	20.93551616292879	0.0
11	2.487082714153366E-4	0.0	0.0	22.535580827079357	0.0
12	2.487082714153366E-4	0.0	0.0	27.052869160796114	0.0
13	2.487082714153366E-4	0.0	0.0	27.81789580366969	0.0
14	2.487082714153366E-4	0.0	0.0	28.178149734814806	0.0
15	2.487082714153366E-4	0.0	0.0	29.326560178075123	0.0
16	2.487082714153366E-4	0.0	0.0	30.151898576766918	0.0
17	2.487082714153366E-4	0.0	0.0	30.96654251978785	0.0
18	2.487082714153366E-4	0.0	0.0	31.862389713425895	0.0
19	2.487082714153366E-4	0.0	0.0	32.637115978884665	0.0
20	2.487082714153366E-4	0.0	0.0	33.203549067033094	0.0
21	2.487082714153366E-4	0.0	0.0	33.835765492970886	0.0
22	2.487082714153366E-4	0.0	0.0	34.5577656048896	0.0
23	2.487082714153366E-4	0.0	0.0	35.22218975197568	0.0
24	2.487082714153366E-4	0.0	0.0	35.769099240818	0.0
25	2.487082714153366E-4	0.0	0.0	36.25333424526366	0.0
26	2.487082714153366E-4	0.0	0.0	36.735330875266584	0.0
27	2.487082714153366E-4	0.0	0.0	37.25214666326765	0.0
28	2.487082714153366E-4	0.0	0.0	37.693728199165584	0.0
29	2.487082714153366E-4	0.0	0.0	38.15309237646971	0.0
30	2.487082714153366E-4	0.0	0.0	38.70224023975477	0.0
31	2.487082714153366E-4	0.0	0.0	39.15886862607333	0.0
32	2.487082714153366E-4	0.0	0.0	39.624450510162845	0.0
33	2.487082714153366E-4	0.0	0.0	40.09699622585198	0.0
34	2.487082714153366E-4	0.0	0.0	40.54777996779228	0.0
35	2.487082714153366E-4	0.0	0.0	41.029527889523784	0.0
36	2.487082714153366E-4	0.0	0.0	41.46066367802227	0.0
37	2.487082714153366E-4	0.0	0.0	41.872648929621775	0.0
38	2.487082714153366E-4	0.0	0.0	42.31970204749084	0.0
39	2.487082714153366E-4	0.0	0.0	42.79635144965834	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACCGA	20	7.0320204E-4	45.000004	17
GCTCTCG	20	7.0320204E-4	45.000004	9
ACGATGG	30	2.1646847E-6	45.000004	2
CGAATAT	60	0.0	45.000004	14
CGGGACG	20	7.0320204E-4	45.000004	6
CATCGAA	20	7.0320204E-4	45.000004	21
GCTACGA	60	0.0	45.000004	10
CCAACCG	20	7.0320204E-4	45.000004	16
GACGATA	20	7.0320204E-4	45.000004	19
AAGATCG	20	7.0320204E-4	45.000004	2
CGGTATT	20	7.0320204E-4	45.000004	9
CGTGCGG	55	1.8189894E-12	45.0	2
TGACCGG	25	3.8897564E-5	45.0	2
CGTTTTT	14595	0.0	43.93628	1
TACGGCT	2925	0.0	43.0	7
ACGGCTG	2965	0.0	42.495785	8
AACACGT	60	3.6379788E-12	41.250004	41
CGGCTGT	3075	0.0	40.97561	9
GGCGACT	840	0.0	40.714287	10
GAATGAC	965	0.0	40.569946	1
>>END_MODULE