FastQCFastQC Report
Sat 18 Jun 2016
SRR3552178_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552178_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481324
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198844.131105035277692No Hit
GAATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTC117452.440144268725432No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCG75821.5752383010196873No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGC50641.0520979631183984No Hit
GAACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCT36490.7581171934081824No Hit
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC21650.4498009656696944TruSeq Adapter, Index 13 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCT17050.3542312454812143No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTC15250.3168343984509395No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTT13420.2788142706368268No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTATACGCTCGTA13370.27777546933043024No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCC8660.17992038626787776No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTATACGCT5130.10658101403628326No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAG253.8874146E-545.0000041
TCTACGT207.0291955E-445.011
CTAGTAC207.0291955E-445.023
CGTTTTT100250.043.832921
TACGGCT4800.043.5937467
CGAACGG650.041.5384642
ACGGCTG5100.041.470598
ACGGGTA556.002665E-1140.9090925
GCGAACG501.0786607E-940.5000041
CAAGGGC1300.039.8076934
ATAGCGG403.452642E-739.3752
TAGGGTA800.039.3755
GCTACGA403.452642E-739.37510
CACCCGT403.452642E-739.37516
CGGCTGT5350.039.1121529
GCGAATG356.2407216E-638.571431
TACGGGA1050.038.571434
CGCACGG356.2407216E-638.571432
TGATACC8650.038.497114
GATACCT8700.038.275865