##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552178_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	481324
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.474491195120127	31.0	30.0	33.0	28.0	34.0
2	30.527719374059885	31.0	30.0	33.0	26.0	34.0
3	30.13653173330231	31.0	30.0	34.0	25.0	34.0
4	34.41935993218705	35.0	33.0	37.0	32.0	37.0
5	35.053971960675135	35.0	35.0	37.0	33.0	37.0
6	34.96437534799844	35.0	35.0	37.0	32.0	37.0
7	35.43565456947918	37.0	35.0	37.0	33.0	37.0
8	35.156574365707925	37.0	35.0	37.0	32.0	37.0
9	37.157758599197216	39.0	37.0	39.0	33.0	39.0
10	36.614153875559914	39.0	35.0	39.0	32.0	39.0
11	36.78541481413767	39.0	37.0	39.0	32.0	39.0
12	37.02358910006565	39.0	37.0	39.0	33.0	39.0
13	36.88868620721178	39.0	37.0	39.0	33.0	39.0
14	38.05867773059311	40.0	37.0	41.0	33.0	41.0
15	38.127753862263255	40.0	37.0	41.0	33.0	41.0
16	38.0197309920137	40.0	37.0	41.0	33.0	41.0
17	37.65229034912034	39.0	36.0	41.0	32.0	41.0
18	37.359628857069254	39.0	36.0	40.0	33.0	41.0
19	36.72646283999967	37.0	36.0	40.0	32.0	41.0
20	36.27776300371475	37.0	35.0	40.0	31.0	41.0
21	36.54868446202558	38.0	35.0	40.0	32.0	41.0
22	36.90759239098819	38.0	35.0	40.0	33.0	41.0
23	37.00102218048549	38.0	35.0	40.0	33.0	41.0
24	36.86676334444158	38.0	35.0	40.0	33.0	41.0
25	36.464686988390355	38.0	35.0	40.0	32.0	41.0
26	36.54876548852748	38.0	35.0	40.0	32.0	41.0
27	36.78003797857576	38.0	35.0	40.0	33.0	41.0
28	36.60944395043671	38.0	35.0	40.0	32.0	41.0
29	36.58795738421521	38.0	35.0	40.0	32.0	41.0
30	36.16156061197862	38.0	35.0	40.0	31.0	41.0
31	35.945597975584015	38.0	35.0	40.0	30.0	41.0
32	35.492715925239544	38.0	35.0	40.0	26.0	41.0
33	34.81640848991532	38.0	35.0	41.0	21.0	41.0
34	34.28480399896951	38.0	34.0	41.0	16.0	41.0
35	33.945834822281874	38.0	34.0	41.0	15.0	41.0
36	33.66065685484206	38.0	34.0	41.0	10.0	41.0
37	33.30279811519891	38.0	33.0	40.0	10.0	41.0
38	33.20595274700617	38.0	33.0	40.0	10.0	41.0
39	33.000122578554155	38.0	33.0	40.0	10.0	41.0
40	32.84258212763128	38.0	32.0	40.0	10.0	41.0
41	32.66509045881776	38.0	31.0	40.0	10.0	41.0
42	32.52155928231296	37.0	31.0	40.0	9.0	41.0
43	32.61050560537185	37.0	31.0	40.0	10.0	41.0
44	32.59263199009399	37.0	31.0	40.0	10.0	41.0
45	32.571924524852285	37.0	31.0	40.0	10.0	41.0
46	32.36413725473901	37.0	31.0	40.0	10.0	41.0
47	32.28348056610516	37.0	31.0	40.0	10.0	41.0
48	32.34171784494436	37.0	31.0	40.0	10.0	41.0
49	32.33574889263781	36.0	31.0	40.0	10.0	41.0
50	32.25762687919156	36.0	31.0	40.0	10.0	41.0
51	30.85995504067946	35.0	27.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	13.0
10	15.0
11	18.0
12	15.0
13	21.0
14	30.0
15	52.0
16	91.0
17	182.0
18	389.0
19	692.0
20	1136.0
21	1627.0
22	2395.0
23	3667.0
24	5682.0
25	9773.0
26	15340.0
27	18437.0
28	17396.0
29	14782.0
30	13453.0
31	13624.0
32	15859.0
33	20245.0
34	29518.0
35	35458.0
36	42113.0
37	57338.0
38	93190.0
39	68760.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.651569421013704	12.61354098278914	15.682367802145746	30.052521794051408
2	43.64565240877247	25.731939400486993	17.646117791757735	12.976290398982806
3	23.98966184939874	25.301875659638828	38.74417232467112	11.964290166291313
4	20.744446568216006	18.745792854709094	45.76626139565033	14.743499181424571
5	23.27351222876898	21.554919347466573	38.559888972916376	16.611679450848076
6	18.904313934065204	29.596695780804616	40.33208400162884	11.166906283501342
7	59.09699080037563	4.642403038286061	32.57203048258553	3.6885756787527737
8	57.65555010761981	8.425925156443476	29.201120243328816	4.71740449260789
9	52.92048599280318	5.911610474441333	29.72613042358162	11.441773109173862
10	28.80242830193383	20.971528533794284	36.4122711520722	13.813772012199681
11	23.786264553606305	19.86998362849141	40.81491884884195	15.52883296906034
12	20.1130631341882	18.8642162036383	44.691309803791206	16.331410858382295
13	18.963525608529803	19.88805877122271	47.516226076405914	13.63218954384157
14	16.7720703725557	24.017917244932725	42.73420814254016	16.47580423997141
15	15.14426872543235	20.571590030831626	48.857734083486385	15.426407160249644
16	18.03546052139515	22.262343037122605	41.41596928472297	18.28622715675927
17	17.91973805586258	21.31994249195968	43.34003706443062	17.42028238774713
18	18.72335474649093	20.72450158313319	43.41753164188779	17.134612028488085
19	17.756854011019605	22.43935477973257	40.8234370195544	18.98035418969343
20	18.855490272664564	22.748917569038735	44.08651968320715	14.309072475089547
21	18.17341333488461	23.90364910122911	42.98954550365243	14.933392060233855
22	17.705744986744897	19.041851227032105	43.38865296556997	19.86375082065303
23	17.34860509760577	23.18085115223841	43.82266415138243	15.647879598773384
24	18.73623588269025	20.872842409686616	41.967780538680806	18.423141168942333
25	16.29505281265842	26.354804663802344	40.27868961448006	17.07145290905918
26	17.012864515378414	20.556215771496955	42.52021507342248	19.910704639702153
27	19.245456283085822	21.094107087949073	41.21984359807531	18.440593030889797
28	14.877504549949721	22.050219810356435	43.23906557744887	19.833210062244973
29	17.720911485818284	20.508846431925274	41.56140146761849	20.208840614637957
30	16.744022737282993	23.331061821143344	44.27724360306156	15.647671838512103
31	18.327155928231296	23.468599114110244	40.05306197073074	18.151182986927726
32	19.019413118813937	23.524278864133098	41.38605180709875	16.07025620995421
33	17.442304975442738	24.7309504616433	37.05840556465084	20.768338998263125
34	18.38491328086694	23.55710498541523	36.78333097871704	21.274650755000792
35	19.716448795406006	24.102891191795965	36.848152180236184	19.332507832561852
36	18.464485460936917	27.338549500959854	33.3002301983695	20.896734839733735
37	18.144326898305508	25.370021025338442	38.32553539819332	18.160116678162737
38	19.029801131877903	28.927915499746533	32.30048782109348	19.74179554728208
39	20.271999734066863	24.879083527935446	34.13147900374799	20.717437734249692
40	21.6650322859446	25.72342122977454	35.096940937912926	17.514605546367935
41	18.241143180061663	26.89477358286726	32.41953445080652	22.444548786264555
42	20.7334352743682	24.10974728041818	36.42951525375838	18.727302191455237
43	20.977345821110106	24.394171078109547	32.880138950062744	21.748344150717607
44	19.501624685243204	24.243337128420773	34.400944062627254	21.85409412370877
45	19.02439936508464	23.667010163631982	34.577540284714665	22.731050186568716
46	21.353807414548204	25.37874695631217	33.76187349893211	19.50557213020751
47	16.964664134761616	24.473535497918242	39.38261960758242	19.179180759737726
48	18.651885216610847	24.90172939641489	35.213494444490614	21.23289094248365
49	18.341699146520845	23.127456765089626	38.90851069134305	19.62233339704648
50	17.60061829453757	23.989038568614905	37.49407883255354	20.91626430429399
51	18.09259459324696	23.260423332308385	34.535780472197516	24.111201602247135
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	260.0
1	779.5
2	1299.0
3	17256.5
4	33214.0
5	21670.5
6	10127.0
7	9709.5
8	9292.0
9	9004.5
10	8717.0
11	8380.5
12	8044.0
13	7602.5
14	7161.0
15	6673.5
16	6186.0
17	5750.5
18	5315.0
19	5012.0
20	4709.0
21	4553.5
22	4398.0
23	4362.5
24	4327.0
25	4305.0
26	4807.0
27	5331.0
28	5706.5
29	6082.0
30	6960.0
31	7838.0
32	8562.0
33	9286.0
34	10368.5
35	11451.0
36	12543.5
37	13636.0
38	15297.5
39	16959.0
40	18039.0
41	19119.0
42	20505.5
43	21892.0
44	23488.5
45	25085.0
46	43557.5
47	62030.0
48	46761.0
49	31492.0
50	31017.5
51	30543.0
52	27284.5
53	24026.0
54	21733.5
55	19441.0
56	17692.5
57	15944.0
58	14276.5
59	12609.0
60	11527.5
61	10446.0
62	9452.0
63	8458.0
64	7566.0
65	6674.0
66	5844.5
67	5015.0
68	4228.5
69	3442.0
70	3005.5
71	2569.0
72	2196.0
73	1823.0
74	1491.0
75	945.0
76	731.0
77	591.0
78	451.0
79	339.0
80	227.0
81	172.0
82	117.0
83	91.5
84	66.0
85	45.5
86	25.0
87	18.5
88	12.0
89	8.5
90	5.0
91	3.5
92	2.0
93	2.0
94	2.0
95	2.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	481324.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.83381626521609
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.56367164317689	32.36680427488958
2	9.134589859633262	7.825386874112744
3	4.138245892176613	5.317705926173348
4	2.6313055605164366	4.5083543566721
5	1.821414398214855	3.900906483797711
6	1.2986416656884863	3.3375467101472878
7	1.0024292915376516	3.005651046481615
8	0.7442021734437553	2.550161532917143
9	0.6042629073290247	2.3294597713575063
>10	2.9840472580086828	19.46936903276067
>50	0.049301585013922136	1.4880548118861123
>100	0.0219118155617333	1.6614763374107004
>500	9.959916164424226E-4	0.29415533050319115
>1k	0.002987974849327268	2.500640275191378
>5k	9.959916164424226E-4	2.697525967761679
>10k+	9.959916164424226E-4	6.746801267937225
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19884	4.131105035277692	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTC	11745	2.440144268725432	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCG	7582	1.5752383010196873	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGC	5064	1.0520979631183984	No Hit
GAACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCT	3649	0.7581171934081824	No Hit
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	2165	0.4498009656696944	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCT	1705	0.3542312454812143	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTC	1525	0.3168343984509395	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTT	1342	0.2788142706368268	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTATACGCTCGTA	1337	0.27777546933043024	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCC	866	0.17992038626787776	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTATACGCT	513	0.10658101403628326	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07583249536694617	0.0
2	0.0	0.0	0.0	0.6642095553099367	0.0
3	0.0	0.0	0.0	0.84849290706468	0.0
4	0.0	0.0	0.0	1.8249661350774116	0.0
5	0.0	0.0	0.0	4.897532639137047	0.0
6	0.0	0.0	0.0	5.581894939791076	0.0
7	0.0	0.0	0.0	6.293266074411415	0.0
8	0.0	0.0	0.0	7.07776882100207	0.0
9	0.0	0.0	0.0	7.3376769078624795	0.0
10	0.0	0.0	0.0	9.48841113262584	0.0
11	0.0	0.0	0.0	10.095486616083969	0.0
12	0.0	0.0	0.0	11.70521312047602	0.0
13	2.077602612793046E-4	0.0	0.0	11.991714520780182	0.0
14	2.077602612793046E-4	0.0	0.0	12.158961531110021	0.0
15	2.077602612793046E-4	0.0	0.0	12.597335682409355	0.0
16	2.077602612793046E-4	0.0	0.0	12.886953486632704	0.0
17	2.077602612793046E-4	0.0	0.0	13.170130722756397	0.0
18	2.077602612793046E-4	0.0	0.0	13.5033781818484	0.0
19	2.077602612793046E-4	0.0	0.0	13.830185072840747	0.0
20	2.077602612793046E-4	0.0	0.0	14.045424703526107	0.0
21	2.077602612793046E-4	0.0	0.0	14.270844587014153	0.0
22	2.077602612793046E-4	0.0	0.0	14.523273304468507	0.0
23	2.077602612793046E-4	0.0	0.0	14.74973198926295	0.0
24	2.077602612793046E-4	0.0	0.0	14.935885183369207	0.0
25	4.155205225586092E-4	0.0	0.0	15.122661658259302	0.0
26	4.155205225586092E-4	0.0	0.0	15.291570750679377	0.0
27	4.155205225586092E-4	0.0	0.0	15.5232234420058	0.0
28	4.155205225586092E-4	0.0	0.0	15.716648245256833	0.0
29	4.155205225586092E-4	0.0	0.0	15.92648610914893	0.0
30	6.232807838379138E-4	0.0	0.0	16.194081325676674	0.0
31	8.310410451172184E-4	0.0	0.0	16.394985498333764	0.0
32	8.310410451172184E-4	0.0	0.0	16.645544373436604	0.0
33	8.310410451172184E-4	0.0	0.0	16.86514696960883	0.0
34	8.310410451172184E-4	0.0	0.0	17.090982373619433	0.0
35	8.310410451172184E-4	0.0	0.0	17.319103140504108	0.0
36	8.310410451172184E-4	0.0	0.0	17.52561684021574	0.0
37	8.310410451172184E-4	0.0	0.0	17.73711678619807	0.0
38	8.310410451172184E-4	0.0	0.0	17.995986071752085	0.0
39	8.310410451172184E-4	0.0	0.0	18.37348646649658	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGAG	25	3.8874146E-5	45.000004	1
TCTACGT	20	7.0291955E-4	45.0	11
CTAGTAC	20	7.0291955E-4	45.0	23
CGTTTTT	10025	0.0	43.83292	1
TACGGCT	480	0.0	43.593746	7
CGAACGG	65	0.0	41.538464	2
ACGGCTG	510	0.0	41.47059	8
ACGGGTA	55	6.002665E-11	40.909092	5
GCGAACG	50	1.0786607E-9	40.500004	1
CAAGGGC	130	0.0	39.807693	4
ATAGCGG	40	3.452642E-7	39.375	2
TAGGGTA	80	0.0	39.375	5
GCTACGA	40	3.452642E-7	39.375	10
CACCCGT	40	3.452642E-7	39.375	16
CGGCTGT	535	0.0	39.112152	9
GCGAATG	35	6.2407216E-6	38.57143	1
TACGGGA	105	0.0	38.57143	4
CGCACGG	35	6.2407216E-6	38.57143	2
TGATACC	865	0.0	38.49711	4
GATACCT	870	0.0	38.27586	5
>>END_MODULE