FastQCFastQC Report
Sat 18 Jun 2016
SRR3552177_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552177_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620975
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204493.293047224123354No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC100631.6205161238375134No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG74131.193767865050928No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC55400.8921454164821451No Hit
GAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT36690.5908450420709368No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC25680.4135432183260196TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCACACTATCGTA20490.3299649744353637No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCT18230.29357059462941343No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCC18100.29147711260517734No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCACACTAT17900.2882563710294295No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTC17530.2822979991142961No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTT10370.16699545070252425No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC7230.11642980796328355No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAA453.8380676E-1045.00000431
ATGTACG351.2107193E-745.0000041
CATAGCG351.2107193E-745.0000041
TACCGCG351.2107193E-745.00000429
TGATCCG253.8887276E-545.04
TCACGAC207.0307794E-445.025
TTAATCG207.0307794E-445.020
ACCGCGA253.8887276E-545.030
CAACCGG253.8887276E-545.02
ACGCTCG207.0307794E-445.01
CGGTCTA207.0307794E-445.031
CCGCGAC253.8887276E-545.031
AGTAGCG406.8066583E-945.01
ATACCGC406.8066583E-945.028
TTCGTTA302.1638843E-644.99999611
GCGATCC302.1638843E-644.99999633
CGTTTTT115750.044.2807771
CCGATGA7350.042.24489618
GGCGAAC650.041.53846432
CGATGAA7500.041.39999819
Produced by FastQC (version 0.11.5)