##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552177_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620975
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.77520834172068	30.0	28.0	33.0	25.0	33.0
2	28.58692700994404	31.0	28.0	33.0	16.0	34.0
3	28.260010467410122	31.0	25.0	33.0	16.0	34.0
4	33.33883650710576	35.0	32.0	35.0	28.0	37.0
5	34.31006240186803	35.0	33.0	35.0	32.0	37.0
6	33.443777929868354	35.0	33.0	37.0	28.0	37.0
7	34.668704859293854	35.0	35.0	37.0	32.0	37.0
8	34.30125045291678	35.0	35.0	37.0	31.0	37.0
9	36.584474415234105	39.0	35.0	39.0	32.0	39.0
10	36.10643423648295	37.0	35.0	39.0	32.0	39.0
11	36.55036515157615	38.0	35.0	39.0	32.0	39.0
12	36.69129997181851	39.0	35.0	39.0	32.0	39.0
13	36.74112967510769	39.0	35.0	39.0	32.0	39.0
14	37.56411771810459	39.0	37.0	41.0	32.0	41.0
15	37.85078465316639	39.0	37.0	41.0	33.0	41.0
16	37.46478199605459	39.0	36.0	41.0	32.0	41.0
17	37.2756310640525	39.0	36.0	40.0	32.0	41.0
18	37.034291235556985	39.0	36.0	40.0	32.0	41.0
19	36.354975643141834	37.0	35.0	40.0	31.0	41.0
20	36.17150932002094	37.0	35.0	40.0	31.0	41.0
21	36.46263215105278	38.0	35.0	40.0	32.0	41.0
22	36.91019123153106	38.0	35.0	40.0	33.0	41.0
23	37.105360119167436	39.0	35.0	40.0	33.0	41.0
24	37.05050605902009	39.0	35.0	40.0	33.0	41.0
25	36.31022343894682	38.0	35.0	40.0	31.0	41.0
26	36.56500181166714	38.0	35.0	40.0	32.0	41.0
27	36.77177503120093	38.0	35.0	40.0	33.0	41.0
28	36.60077297797818	38.0	35.0	40.0	32.0	41.0
29	36.60132050404606	38.0	35.0	40.0	31.0	41.0
30	36.06539715769556	38.0	35.0	40.0	30.0	41.0
31	35.99506582390595	38.0	35.0	40.0	30.0	41.0
32	35.4322847135553	38.0	35.0	40.0	25.0	41.0
33	34.944914046459196	38.0	35.0	40.0	21.0	41.0
34	34.49849349812794	39.0	35.0	40.0	17.0	41.0
35	34.12679093361246	39.0	34.0	40.0	15.0	41.0
36	33.93360602278675	39.0	34.0	40.0	12.0	41.0
37	33.894941020169895	39.0	34.0	40.0	10.0	41.0
38	33.67392407101735	39.0	33.0	40.0	10.0	41.0
39	33.5263480816458	38.0	33.0	40.0	10.0	41.0
40	33.37513104392286	38.0	33.0	40.0	10.0	41.0
41	33.251208180683605	38.0	33.0	40.0	10.0	41.0
42	33.358141632110794	38.0	33.0	40.0	10.0	41.0
43	33.34208784572648	38.0	33.0	40.0	10.0	41.0
44	33.378635210757274	38.0	33.0	40.0	10.0	41.0
45	33.38138250332139	38.0	33.0	40.0	10.0	41.0
46	33.18248560731109	38.0	33.0	40.0	10.0	41.0
47	33.00398083658762	38.0	32.0	40.0	10.0	41.0
48	33.04430613148678	38.0	33.0	40.0	10.0	41.0
49	33.060113531140544	38.0	33.0	40.0	10.0	41.0
50	33.01878497524055	38.0	33.0	40.0	10.0	41.0
51	32.07169692821772	36.0	31.0	40.0	9.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	9.0
10	15.0
11	21.0
12	18.0
13	20.0
14	28.0
15	51.0
16	72.0
17	186.0
18	449.0
19	875.0
20	1734.0
21	2741.0
22	3987.0
23	5385.0
24	7924.0
25	13148.0
26	18870.0
27	21402.0
28	19534.0
29	17136.0
30	15816.0
31	17026.0
32	20170.0
33	26770.0
34	35872.0
35	47000.0
36	65619.0
37	102264.0
38	132566.0
39	44261.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.83091106727324	13.872056040903418	17.75771971496437	28.539313176858972
2	42.00330126011514	25.061556423366483	19.322839083699023	13.612303232819356
3	24.962357582833448	24.456862192519825	37.79234268690366	12.788437537743066
4	21.46028423044406	20.03462297193929	43.8875961190064	14.61749667861025
5	24.411932847538147	23.391118805104874	36.80083739280969	15.396110954547284
6	19.75055356495833	31.28821611175973	37.363500946092834	11.597729377189097
7	62.53810539876806	4.408228994726036	29.865453520673135	3.188212085832763
8	62.23535569064778	8.506300575707556	25.045774789645314	4.212568943999355
9	56.70936833205845	7.0526188654937805	26.143886629896535	10.09412617255123
10	27.580659446837636	28.17923426869037	32.686017955634284	11.554088328837715
11	22.04919682756955	20.955594025524377	42.63295623817384	14.362252908732234
12	18.16707596924192	19.985345625830348	44.793912798421836	17.053665606505895
13	19.03667619469383	21.154635854905592	46.481420347034906	13.327267603365675
14	15.82897862232779	26.313458673859657	42.05386690285438	15.80369580095817
15	14.895929787833648	23.063891461008897	46.491726720077295	15.548452031080156
16	17.128869922299607	23.544909215346834	41.339989532589875	17.986231329763676
17	17.459479045050124	24.115302548411773	42.29558355811426	16.129634848423848
18	17.368171021377673	22.281251258102177	43.067595313821	17.282982406699144
19	16.65799750392528	23.990820886509116	41.324046861789924	18.027134747775676
20	18.678851805628245	23.90869197632755	43.90853093924876	13.503925278795442
21	17.618100567655702	25.759974234067396	42.20991183219937	14.41201336607754
22	16.953178469342568	21.27251499657796	43.60078908168606	18.173517452393416
23	16.657031281452557	24.492290349853054	43.144248963323804	15.706429405370587
24	16.987157292966707	23.99388059100608	42.22037924232055	16.79858287370667
25	15.50384476025605	27.768750754861305	40.31933652723539	16.408067957647248
26	16.54060147348927	23.3907967309473	42.68674262248883	17.381859173074602
27	18.727162929264463	23.27533314545674	40.55445066226498	17.44305326301381
28	15.366158057892829	25.215990981923586	42.250332138975	17.16751882120858
29	18.80494383831877	21.650630057570755	41.33982849551109	18.20459760859938
30	16.211763758605418	25.93292805668505	40.6462417971738	17.209066387535728
31	17.55159225411651	25.11647006723298	38.34019082893836	18.991746849712147
32	18.414750996416927	25.528402914771124	37.92890212971537	18.127943959096584
33	16.679254398325213	26.881275413663996	36.27956036877491	20.15990981923588
34	18.568863480816457	25.5338781754499	36.29308748339305	19.604170860340595
35	17.297636780868793	26.369982688514032	36.311928821611176	20.020451709006
36	18.42554048069568	30.875156004670075	33.35673738878377	17.342566125850478
37	16.96461210193647	27.18305889931157	35.49997987036515	20.352349128386813
38	17.651918354201054	30.064817424211927	33.220661057208424	19.062603164378597
39	19.36857361407464	27.21140142517815	33.283465517935504	20.13655944281171
40	18.214742944562985	27.286766778050648	35.7982205402794	18.70026973710697
41	17.21888964934176	29.317766415717216	33.49233060912275	19.97101332581827
42	18.146624260235917	26.721365594428114	36.62369660614356	18.5083135391924
43	19.476951568098556	27.548935142316516	33.40214984500181	19.571963444583115
44	18.52377309875599	26.320222231168728	34.05032408712106	21.105680582954225
45	18.108297435484523	25.86883529932767	34.68142839888884	21.341438866298965
46	20.15556181810862	27.35456338822014	33.76287290148557	18.727001892185676
47	16.717420185997824	26.760336567494665	37.814082692539955	18.708160553967552
48	17.832279882442933	26.65083135391924	34.86452755747011	20.652361206167722
49	17.73855630258867	25.096662506542135	37.80087765207939	19.363903538789806
50	17.48991505294094	25.770602681267363	36.101614396714844	20.637867869076857
51	17.701517774467572	24.780224646724907	34.69141269777366	22.826844881033857
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	310.0
1	843.0
2	1376.0
3	18220.0
4	35064.0
5	23490.0
6	11916.0
7	11463.5
8	11011.0
9	10659.0
10	10307.0
11	10012.0
12	9717.0
13	9415.5
14	9114.0
15	8534.0
16	7954.0
17	7447.5
18	6941.0
19	6550.0
20	6159.0
21	5939.5
22	5720.0
23	5891.0
24	6062.0
25	6868.0
26	8349.0
27	9024.0
28	10357.5
29	11691.0
30	13360.0
31	15029.0
32	16846.0
33	18663.0
34	20353.5
35	22044.0
36	22909.0
37	23774.0
38	25391.5
39	27009.0
40	29057.0
41	31105.0
42	32756.5
43	34408.0
44	34847.5
45	35287.0
46	53956.5
47	72626.0
48	58008.5
49	43391.0
50	41307.5
51	39224.0
52	34266.0
53	29308.0
54	25727.5
55	22147.0
56	19303.0
57	16459.0
58	14043.5
59	11628.0
60	10275.5
61	8923.0
62	7573.0
63	6223.0
64	5323.5
65	4424.0
66	3760.5
67	3097.0
68	2593.0
69	2089.0
70	1697.5
71	1306.0
72	1141.0
73	976.0
74	828.0
75	606.0
76	532.0
77	412.0
78	292.0
79	223.0
80	154.0
81	110.0
82	66.0
83	52.5
84	39.0
85	29.5
86	20.0
87	12.0
88	4.0
89	4.5
90	5.0
91	3.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	620975.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.02476861606541
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.0642512960602	31.20518309375123
2	8.45159431411296	6.934494023466765
3	3.7797981989537677	4.651960395824972
4	2.352231747005029	3.859990526089785
5	1.6592138942991437	3.4034433049091595
6	1.2663273915532194	3.117047293839389
7	0.9560143435026999	2.745418706508656
8	0.7965449030000362	2.6142456270306202
9	0.6637336997837628	2.4506569310682504
>10	3.9218274099051986	25.306342193360393
>50	0.05777975799850105	1.5853897138474575
>100	0.02470582749586827	2.0153314449620345
>500	0.0011954432659291097	0.30782520367160215
>1k	0.003187848709144293	2.6971896098660455
>5k	7.969621772860732E-4	2.11750391033365
>10k+	7.969621772860732E-4	4.987978021469955
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20449	3.293047224123354	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	10063	1.6205161238375134	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG	7413	1.193767865050928	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	5540	0.8921454164821451	No Hit
GAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	3669	0.5908450420709368	No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	2568	0.4135432183260196	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCACACTATCGTA	2049	0.3299649744353637	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCT	1823	0.29357059462941343	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCC	1810	0.29147711260517734	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCACACTAT	1790	0.2882563710294295	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTC	1753	0.2822979991142961	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTT	1037	0.16699545070252425	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	723	0.11642980796328355	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.610370787873908E-4	0.0	0.0	0.10048713716333185	0.0
2	1.610370787873908E-4	0.0	0.0	0.6486573533556101	0.0
3	1.610370787873908E-4	0.0	0.0	0.7943959096581988	0.0
4	1.610370787873908E-4	0.0	0.0	1.5912073754982086	0.0
5	1.610370787873908E-4	0.0	0.0	3.6602117637586056	0.0
6	1.610370787873908E-4	0.0	0.0	4.202262570956963	0.0
7	1.610370787873908E-4	0.0	0.0	4.834333105197472	0.0
8	1.610370787873908E-4	0.0	0.0	5.616812271025403	0.0
9	1.610370787873908E-4	0.0	0.0	5.904102419582109	0.0
10	1.610370787873908E-4	0.0	0.0	7.64201457385563	0.0
11	1.610370787873908E-4	0.0	0.0	8.41789121945328	0.0
12	1.610370787873908E-4	0.0	0.0	9.985265107290953	0.0
13	1.610370787873908E-4	0.0	0.0	10.308788598574822	0.0
14	1.610370787873908E-4	0.0	0.0	10.477233382986432	0.0
15	1.610370787873908E-4	0.0	0.0	10.98482225532429	0.0
16	1.610370787873908E-4	0.0	0.0	11.344095978098958	0.0
17	1.610370787873908E-4	0.0	0.0	11.721244816619027	0.0
18	1.610370787873908E-4	0.0	0.0	12.099037803454245	0.0
19	1.610370787873908E-4	0.0	0.0	12.681186843270662	0.0
20	1.610370787873908E-4	0.0	0.0	12.985546922178832	0.0
21	1.610370787873908E-4	0.0	0.0	13.271226699947663	0.0
22	1.610370787873908E-4	0.0	0.0	13.598776118201215	0.0
23	1.610370787873908E-4	0.0	0.0	13.900076492612424	0.0
24	1.610370787873908E-4	0.0	0.0	14.138089295060187	0.0
25	1.610370787873908E-4	0.0	0.0	14.363058094126172	0.0
26	1.610370787873908E-4	0.0	0.0	14.578203631386126	0.0
27	1.610370787873908E-4	0.0	0.0	14.831353919239906	0.0
28	3.220741575747816E-4	0.0	0.0	15.060831756511936	0.0
29	3.220741575747816E-4	0.0	0.0	15.302387374693023	0.0
30	3.220741575747816E-4	0.0	0.0	15.603043600789082	0.0
31	4.831112363621724E-4	0.0	0.0	15.88083256169733	0.0
32	4.831112363621724E-4	0.0	0.0	16.15362937316317	0.0
33	4.831112363621724E-4	0.0	0.0	16.426748258786585	0.0
34	4.831112363621724E-4	0.0	0.0	16.69487499496759	0.0
35	4.831112363621724E-4	0.0	0.0	16.970087362615242	0.0
36	4.831112363621724E-4	0.0	0.0	17.23257780103869	0.0
37	4.831112363621724E-4	0.0	0.0	17.48862675631064	0.0
38	4.831112363621724E-4	0.0	0.0	17.78251942509763	0.0
39	4.831112363621724E-4	0.0	0.0	18.077056242199767	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	45	3.8380676E-10	45.000004	31
ATGTACG	35	1.2107193E-7	45.000004	1
CATAGCG	35	1.2107193E-7	45.000004	1
TACCGCG	35	1.2107193E-7	45.000004	29
TGATCCG	25	3.8887276E-5	45.0	4
TCACGAC	20	7.0307794E-4	45.0	25
TTAATCG	20	7.0307794E-4	45.0	20
ACCGCGA	25	3.8887276E-5	45.0	30
CAACCGG	25	3.8887276E-5	45.0	2
ACGCTCG	20	7.0307794E-4	45.0	1
CGGTCTA	20	7.0307794E-4	45.0	31
CCGCGAC	25	3.8887276E-5	45.0	31
AGTAGCG	40	6.8066583E-9	45.0	1
ATACCGC	40	6.8066583E-9	45.0	28
TTCGTTA	30	2.1638843E-6	44.999996	11
GCGATCC	30	2.1638843E-6	44.999996	33
CGTTTTT	11575	0.0	44.280777	1
CCGATGA	735	0.0	42.244896	18
GGCGAAC	65	0.0	41.538464	32
CGATGAA	750	0.0	41.399998	19
>>END_MODULE