FastQCFastQC Report
Sat 18 Jun 2016
SRR3552174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398686
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136983.435786558845808No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC64171.6095373301294753No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCG64131.6085340343026844No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGC43621.0940940991155947No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC24420.6125121022559107Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC17930.4497273543590695No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCT15710.3940444359721686No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTC13460.33760904571517436No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT12770.32030219270302945No Hit
GAACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT12130.3042494594743733No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTA12020.30149039595069804No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGCT11240.2819261273282734Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC9800.24580747756379706Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGC6590.16529298746381862No Hit
TCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC5420.13594658453018163Illumina Single End Adapter 1 (95% over 21bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5090.12766939395915583No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4980.12491033043548055No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCG4140.10384111807286936No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC4060.10183452641928736No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTGT351.2092642E-745.0000046
CGGGTAT351.2092642E-745.0000046
TCGTTGG302.1619217E-645.0000042
GCGACAG351.2092642E-745.0000041
CACGGGT351.2092642E-745.0000044
CGCGGGT302.1619217E-645.0000044
GTTCGGG351.2092642E-745.0000043
ACGCGAG253.886206E-545.01
TACCCGC207.027736E-445.031
CAAACGT207.027736E-445.018
CTACTAG253.886206E-545.012
GATCGCG207.027736E-445.01
TTAGCCG253.886206E-545.01
GACGTTG253.886206E-545.01
GGCCGAT502.1827873E-1145.08
TAAACGG207.027736E-445.02
TCGATTG502.1827873E-1145.01
CGACAGG453.8380676E-1045.02
CCTCGTT207.027736E-445.014
ACGGGTA207.027736E-445.05
Produced by FastQC (version 0.11.5)