##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552174_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	398686
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.433258253362297	31.0	30.0	33.0	28.0	34.0
2	30.31744029135711	31.0	30.0	33.0	25.0	34.0
3	29.942478542010505	31.0	28.0	34.0	25.0	34.0
4	34.41173755787763	35.0	33.0	37.0	32.0	37.0
5	35.07617272740954	35.0	35.0	37.0	33.0	37.0
6	34.99638562678398	35.0	35.0	37.0	32.0	37.0
7	35.53078362420551	37.0	35.0	37.0	33.0	37.0
8	35.01548587108652	37.0	35.0	37.0	32.0	37.0
9	36.94716895000075	39.0	37.0	39.0	32.0	39.0
10	36.57602724951465	38.0	35.0	39.0	32.0	39.0
11	36.91543219475979	39.0	37.0	39.0	33.0	39.0
12	37.132164159263176	39.0	37.0	39.0	34.0	39.0
13	37.05570047606387	39.0	37.0	39.0	33.0	39.0
14	38.103326928961636	40.0	37.0	41.0	33.0	41.0
15	38.29146245416192	40.0	38.0	41.0	34.0	41.0
16	38.00157015796893	39.0	37.0	41.0	33.0	41.0
17	37.822770300436936	39.0	37.0	41.0	33.0	41.0
18	37.61445097144119	39.0	37.0	40.0	33.0	41.0
19	36.952965491640036	38.0	36.0	40.0	32.0	41.0
20	36.779917027435125	38.0	35.0	40.0	32.0	41.0
21	36.955767195236355	38.0	35.0	40.0	33.0	41.0
22	37.299526444369754	39.0	35.0	40.0	33.0	41.0
23	37.36300998780995	39.0	35.0	40.0	33.0	41.0
24	37.312366122713115	39.0	35.0	41.0	33.0	41.0
25	36.82361306893144	38.0	35.0	40.0	32.0	41.0
26	37.01975238658995	39.0	35.0	40.0	33.0	41.0
27	37.22474328168032	39.0	35.0	40.0	33.0	41.0
28	37.09065530266927	39.0	35.0	41.0	33.0	41.0
29	37.03470651088827	39.0	35.0	41.0	33.0	41.0
30	36.67539115996047	39.0	35.0	41.0	32.0	41.0
31	36.519022488875954	39.0	35.0	41.0	31.0	41.0
32	36.208392067943194	39.0	35.0	41.0	30.0	41.0
33	35.783428562828895	39.0	35.0	41.0	27.0	41.0
34	35.41532183221884	39.0	35.0	41.0	23.0	41.0
35	35.22449245772362	39.0	35.0	41.0	21.0	41.0
36	34.94690307660665	39.0	35.0	41.0	18.0	41.0
37	34.64296965531772	39.0	35.0	41.0	17.0	41.0
38	34.56652102155581	39.0	35.0	41.0	18.0	41.0
39	34.49666905785505	39.0	35.0	41.0	17.0	41.0
40	34.448031784411796	39.0	35.0	41.0	16.0	41.0
41	34.29547062098995	39.0	34.0	41.0	15.0	41.0
42	34.183675875250195	39.0	34.0	41.0	15.0	41.0
43	34.25037749005483	39.0	34.0	41.0	15.0	41.0
44	34.245526554732294	39.0	34.0	41.0	15.0	41.0
45	34.214446958257874	39.0	34.0	40.0	15.0	41.0
46	34.086938091631005	38.0	34.0	40.0	15.0	41.0
47	34.072540796516556	38.0	34.0	40.0	15.0	41.0
48	34.08165824734252	38.0	34.0	40.0	15.0	41.0
49	34.04712480498437	38.0	34.0	40.0	15.0	41.0
50	33.96642972163557	38.0	34.0	40.0	15.0	41.0
51	32.68084908925821	37.0	31.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	9.0
10	14.0
11	18.0
12	10.0
13	9.0
14	14.0
15	25.0
16	47.0
17	102.0
18	201.0
19	340.0
20	655.0
21	1044.0
22	1524.0
23	2469.0
24	3682.0
25	5739.0
26	8920.0
27	11250.0
28	11053.0
29	9712.0
30	8837.0
31	9417.0
32	10832.0
33	14641.0
34	23705.0
35	29205.0
36	35760.0
37	54711.0
38	94302.0
39	60433.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.15766793918021	17.00937579950136	19.43534510868202	23.39761115263641
2	40.435831707158016	27.779254852189446	18.222109630134995	13.56280381051755
3	25.73428713323267	25.5855485269109	35.69601139744059	12.984152942415836
4	23.447525120019264	20.815127694476352	40.50982477438385	15.227522411120532
5	24.327917208028374	23.93211700435932	34.03380103640459	17.706164751207716
6	18.749592411070363	33.20407538764842	35.63807106344341	12.408261137837798
7	59.55865016579464	6.8276789252695105	28.959632392409063	4.654038516526791
8	57.218462649804614	10.737271938317372	25.946735024555668	6.097530387322354
9	50.368460392389004	9.412670622996544	27.91896379607009	12.299905188544368
10	28.805375659039946	21.866330896996633	35.19561760382858	14.132675840134842
11	22.516717416713906	22.55183277065159	38.076330746502265	16.855119066132243
12	20.24425236903227	20.050616274461603	42.565828747435326	17.139302609070796
13	18.59408155791776	22.850564103078614	44.31758326101243	14.237771077991201
14	14.861319434341812	27.138650466783382	40.227898647055575	17.772131451819227
15	14.014537756530201	22.961428291939022	47.13784783012195	15.886186121408826
16	14.058181124995611	24.488444540314937	40.67787682537134	20.77549750931811
17	14.057679477082216	23.40889823068781	41.65784602418946	20.875576268040515
18	15.538544117425742	23.018365330109408	42.79382772407358	18.649262828391265
19	16.15607269881561	24.219310434778247	40.09721936561605	19.527397500790094
20	16.449035080238584	24.97855455170234	42.477037066764325	16.095373301294753
21	15.520735616500204	27.603427258544315	41.07994762795784	15.795889496997637
22	14.49787552108677	23.330891980154806	39.46539381869441	22.705838680064012
23	14.615511956778015	26.138364527472746	40.535408817967024	18.710714697782215
24	16.035928023557386	23.019619449892897	40.57829971456234	20.36615281198738
25	13.845733233672613	28.69300652643935	38.43350406083986	19.027756179048172
26	13.639054293353666	24.28151477603929	40.0583416523279	22.021089278279145
27	16.502711406971905	25.21884390221879	37.60227346834351	20.6761712224658
28	12.816602539341737	24.83483242451453	41.39172180613315	20.956843230010584
29	15.51772572901983	23.746507276402983	37.567408938362526	23.168358056214664
30	14.573373532052795	26.816090858470076	37.904767160121	20.705768449356135
31	16.190184756926502	27.447665581435015	35.66867158616054	20.693478075477945
32	17.161876765173595	29.345399637810203	34.02953702914073	19.46318656787547
33	15.080288748538951	27.82089162900127	33.83865999809374	23.260159624366043
34	15.779084291898887	26.020728091781503	36.54730790647277	21.652879709846847
35	17.48393472557351	25.200282929423157	32.71145713669404	24.604325208309298
36	16.421946092915228	28.45673035923007	31.94945395624627	23.171869591608434
37	15.628840741836934	29.601240073641915	34.63377194082561	20.13614724369554
38	15.628589917880237	32.77491559773857	30.85611232900077	20.740382155380424
39	17.923629121664668	28.078989480443255	34.17250668445845	19.82487471343363
40	19.387688556909445	26.310178937810708	35.24051509207747	19.06161741320237
41	16.725693904476206	26.235684222671473	32.91086218226875	24.127759690583567
42	18.466913811871997	24.329171327811864	34.82790968330967	22.376005177006466
43	18.261990639249937	24.78366433734819	32.743311779194656	24.21103324420722
44	16.791409781131016	26.200568868733793	33.77946554431307	23.228555805822126
45	16.858379777569315	25.117761847669595	33.80504958789624	24.21880878686485
46	19.670617980064513	26.944261900342624	32.871482821067204	20.513637298525655
47	15.5769201828005	24.48593630074796	39.27602173138761	20.661121785063933
48	16.859383073396106	25.07737919064126	35.28766999593665	22.775567740025984
49	17.03947467430509	22.965190651289486	38.55063884861771	21.44469582578771
50	16.725443080519504	23.07279412871282	37.278961388160106	22.922801402607565
51	16.830538318375865	22.406605699723592	33.46217323909041	27.300682742810135
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	556.0
1	1107.0
2	1658.0
3	11747.5
4	21837.0
5	14255.0
6	6673.0
7	6311.5
8	5950.0
9	5785.0
10	5620.0
11	5530.0
12	5440.0
13	5151.0
14	4862.0
15	4477.5
16	4093.0
17	3715.0
18	3337.0
19	3238.5
20	3140.0
21	3184.5
22	3229.0
23	3330.5
24	3432.0
25	3842.0
26	4586.0
27	4920.0
28	5831.0
29	6742.0
30	7588.5
31	8435.0
32	8865.0
33	9295.0
34	10268.5
35	11242.0
36	11846.5
37	12451.0
38	12854.0
39	13257.0
40	15767.5
41	18278.0
42	20919.0
43	23560.0
44	25442.0
45	27324.0
46	41737.5
47	56151.0
48	47384.5
49	38618.0
50	36419.0
51	34220.0
52	27667.5
53	21115.0
54	17066.5
55	13018.0
56	10649.5
57	8281.0
58	6852.0
59	5423.0
60	4677.0
61	3931.0
62	3221.5
63	2512.0
64	2215.0
65	1918.0
66	1544.5
67	1171.0
68	1024.0
69	877.0
70	781.5
71	686.0
72	577.0
73	468.0
74	380.0
75	243.5
76	195.0
77	147.0
78	99.0
79	77.0
80	55.0
81	46.0
82	37.0
83	27.5
84	18.0
85	13.0
86	8.0
87	7.0
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	398686.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.94523112668972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.40625787265515	32.88768608247356
2	9.709610914881706	8.145437479498847
3	3.4493425041079018	4.340504057097619
4	1.7705886746762798	2.970710047583832
5	1.1528693283047622	2.4178685217307394
6	0.783516145254606	1.9718859482518465
7	0.5762276053938407	1.6919000062876526
8	0.4598784289991481	1.543176559563859
9	0.37557509091399527	1.4178225594433531
>10	3.183402271151667	25.320898065238822
>50	0.07697195335590533	2.165962701154226
>100	0.04606195633896696	3.5468910336272024
>500	0.002424313491524577	0.6838544209043274
>1k	0.005454705355930298	4.151428510545601
>5k	0.0012121567457622886	3.2616551004470336
>10k+	6.060783728811443E-4	3.4823189061514777
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13698	3.435786558845808	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTC	6417	1.6095373301294753	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCG	6413	1.6085340343026844	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGC	4362	1.0940940991155947	No Hit
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	2442	0.6125121022559107	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCC	1793	0.4497273543590695	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCT	1571	0.3940444359721686	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTC	1346	0.33760904571517436	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTT	1277	0.32030219270302945	No Hit
GAACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT	1213	0.3042494594743733	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTA	1202	0.30149039595069804	No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGCT	1124	0.2819261273282734	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	980	0.24580747756379706	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGC	659	0.16529298746381862	No Hit
TCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC	542	0.13594658453018163	Illumina Single End Adapter 1 (95% over 21bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	509	0.12766939395915583	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	498	0.12491033043548055	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCG	414	0.10384111807286936	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	406	0.10183452641928736	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.30149039595069804	0.0
2	0.0	0.0	0.0	1.4660660268983612	0.0
3	0.0	0.0	0.0	1.9228164520449678	0.0
4	0.0	0.0	0.0	2.777373672514209	0.0
5	0.0	0.0	0.0	5.829901225525853	0.0
6	0.0	0.0	0.0	7.021315019840175	0.0
7	0.0	0.0	0.0	8.237811209824272	0.0
8	0.0	0.0	0.0	9.750279668711718	0.0
9	0.0	0.0	0.0	10.266224547638995	0.0
10	0.0	0.0	0.0	13.1108190405482	0.0
11	0.0	0.0	0.0	15.039153619640519	0.0
12	0.0	0.0	0.0	17.65474584008468	0.0
13	0.0	0.0	0.0	18.34852490431066	0.0
14	0.0	0.0	0.0	18.68337488650216	0.0
15	0.0	0.0	0.0	19.430579453504762	0.0
16	0.0	0.0	0.0	20.32376356330546	0.0
17	0.0	0.0	0.0	21.34662365871889	0.0
18	0.0	0.0	0.0	22.500664683485248	0.0
19	0.0	0.0	0.0	23.203473410152352	0.0
20	0.0	0.0	0.0	23.81272480097119	0.0
21	0.0	0.0	0.0	24.497975850669448	0.0
22	0.0	0.0	0.0	25.215834014738416	0.0
23	0.0	0.0	0.0	25.928926523630125	0.0
24	0.0	0.0	0.0	26.485755707499134	0.0
25	0.0	0.0	0.0	26.998189051032643	0.0
26	0.0	0.0	0.0	27.472246329191393	0.0
27	0.0	0.0	0.0	28.00825712465449	0.0
28	0.0	0.0	0.0	28.519436348404508	0.0
29	0.0	0.0	0.0	29.005031528571358	0.0
30	0.0	0.0	0.0	29.556593409349713	0.0
31	0.0	0.0	0.0	30.04795754052061	0.0
32	0.0	0.0	0.0	30.53330189673076	0.0
33	0.0	0.0	0.0	31.013378949850257	0.0
34	0.0	0.0	0.0	31.472386790607146	0.0
35	0.0	0.0	0.0	31.96174433012446	0.0
36	0.0	0.0	0.0	32.4034453178692	0.0
37	0.0	0.0	0.0	32.8614498627993	0.0
38	0.0	0.0	0.0	33.37212743863592	0.0
39	0.0	0.0	0.0	33.860481682326444	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTGT	35	1.2092642E-7	45.000004	6
CGGGTAT	35	1.2092642E-7	45.000004	6
TCGTTGG	30	2.1619217E-6	45.000004	2
GCGACAG	35	1.2092642E-7	45.000004	1
CACGGGT	35	1.2092642E-7	45.000004	4
CGCGGGT	30	2.1619217E-6	45.000004	4
GTTCGGG	35	1.2092642E-7	45.000004	3
ACGCGAG	25	3.886206E-5	45.0	1
TACCCGC	20	7.027736E-4	45.0	31
CAAACGT	20	7.027736E-4	45.0	18
CTACTAG	25	3.886206E-5	45.0	12
GATCGCG	20	7.027736E-4	45.0	1
TTAGCCG	25	3.886206E-5	45.0	1
GACGTTG	25	3.886206E-5	45.0	1
GGCCGAT	50	2.1827873E-11	45.0	8
TAAACGG	20	7.027736E-4	45.0	2
TCGATTG	50	2.1827873E-11	45.0	1
CGACAGG	45	3.8380676E-10	45.0	2
CCTCGTT	20	7.027736E-4	45.0	14
ACGGGTA	20	7.027736E-4	45.0	5
>>END_MODULE