FastQCFastQC Report
Sat 18 Jun 2016
SRR3552172_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552172_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434562
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401919.24862275118395No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCG91792.112241751464693No Hit
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC79621.8321896530299475No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGC66131.521762142110907No Hit
GAATGACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCT26570.6114202346270498No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC23450.5396238051187172TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC22710.5225951647866127No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTA21320.4906089349736056No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTC20520.47219959407403317No Hit
GAACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT17570.4043151495068598No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT10080.2319576953346128TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGC9340.21492905500250825No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC7680.17672967263589545TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATGTGCTTT6980.16062149934876957No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGT6100.14037122435923988No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6030.13876040703052728No Hit
GAATGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT5930.13645923941808075No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA5720.13162678743194298No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCG5230.12035106613095484No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA5040.1159788476673064No Hit
TCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC4980.11459814709983845TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA4900.11275721300988123No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTAT4850.11160662920365794No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4650.10700429397876483No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGGC302.1623728E-645.0000044
CGGGTGA207.02844E-445.06
CCGGGCG207.02844E-445.05
CCCTCGT406.7975634E-945.014
TTGGACG253.886788E-545.01
GGATCAC207.02844E-445.08
TACGGGA253.886788E-545.04
TAACGCT207.02844E-445.036
TGTGCGG207.02844E-445.02
AATGCGG253.886788E-545.02
GCCCTCG453.8380676E-1045.013
GCGATCG253.886788E-545.09
ACGCTAA207.02844E-445.038
GTATCCG207.02844E-445.09
TAGTGCG253.886788E-545.01
AGCTACG351.2095916E-745.09
TTCGATG253.886788E-545.01
TGCGTAG253.886788E-545.01
CGTTTTT165650.044.3208581
TACGGCT7250.042.2068987
Produced by FastQC (version 0.11.5)