##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552172_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	434562
Sequences flagged as poor quality	0
Sequence length	51
%GC	31
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.862594980693203	31.0	30.0	33.0	28.0	34.0
2	30.907177802016744	31.0	30.0	34.0	27.0	34.0
3	30.552758869850564	31.0	30.0	34.0	25.0	34.0
4	34.543291866292954	35.0	35.0	37.0	32.0	37.0
5	35.277171036583965	35.0	35.0	37.0	33.0	37.0
6	35.25911147316148	37.0	35.0	37.0	33.0	37.0
7	35.73255369774623	37.0	35.0	37.0	35.0	37.0
8	35.22753255001588	37.0	35.0	37.0	32.0	37.0
9	37.24303781738854	39.0	37.0	39.0	33.0	39.0
10	37.06686502731486	39.0	37.0	39.0	33.0	39.0
11	37.32567044518389	39.0	37.0	39.0	34.0	39.0
12	37.51085000529269	39.0	37.0	39.0	35.0	39.0
13	37.42432380189708	39.0	37.0	39.0	34.0	39.0
14	38.60987845232671	40.0	38.0	41.0	34.0	41.0
15	38.7059798141577	40.0	38.0	41.0	34.0	41.0
16	38.55763274285373	40.0	38.0	41.0	34.0	41.0
17	38.26659026790193	40.0	38.0	41.0	33.0	41.0
18	37.695201145061006	39.0	37.0	39.0	34.0	41.0
19	36.75677578803485	37.0	37.0	39.0	33.0	41.0
20	36.03190338777896	35.0	35.0	39.0	32.0	41.0
21	36.17762712800475	35.0	35.0	39.0	33.0	41.0
22	36.446104353348886	35.0	35.0	40.0	33.0	41.0
23	36.48150781706638	35.0	35.0	40.0	33.0	41.0
24	36.32172164156093	35.0	35.0	40.0	33.0	41.0
25	35.883963623142385	35.0	35.0	40.0	32.0	41.0
26	36.024392376691935	35.0	35.0	40.0	33.0	41.0
27	36.24410095682549	35.0	35.0	40.0	33.0	41.0
28	36.03824770688647	35.0	35.0	40.0	33.0	41.0
29	35.856170120719256	35.0	35.0	40.0	32.0	41.0
30	35.35605276117102	35.0	35.0	40.0	31.0	41.0
31	34.93058527897055	35.0	35.0	40.0	26.0	41.0
32	34.08763996852003	35.0	34.0	40.0	21.0	41.0
33	32.977793732539894	35.0	33.0	40.0	15.0	41.0
34	31.95611213129542	35.0	31.0	40.0	10.0	41.0
35	31.375566662524566	35.0	27.0	40.0	7.0	41.0
36	30.945865031917194	35.0	23.0	40.0	7.0	41.0
37	30.542675153372823	35.0	22.0	40.0	7.0	41.0
38	30.46849011188277	35.0	22.0	40.0	7.0	41.0
39	30.348978971930357	35.0	22.0	40.0	7.0	41.0
40	30.250367036234184	35.0	22.0	40.0	7.0	41.0
41	30.03863200187775	35.0	21.0	40.0	7.0	41.0
42	29.86058606136754	35.0	20.0	40.0	7.0	41.0
43	29.85402083016923	35.0	20.0	40.0	7.0	41.0
44	29.76129298005808	35.0	18.0	40.0	7.0	41.0
45	29.748259166701185	35.0	20.0	40.0	7.0	41.0
46	29.669683497406584	35.0	20.0	40.0	7.0	41.0
47	29.728471426401757	35.0	20.0	40.0	7.0	41.0
48	29.735137908975013	35.0	20.0	40.0	7.0	41.0
49	29.702896249557025	35.0	20.0	40.0	7.0	41.0
50	29.612504084572514	35.0	20.0	40.0	7.0	41.0
51	28.57452331312908	35.0	20.0	39.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	9.0
10	7.0
11	9.0
12	4.0
13	4.0
14	9.0
15	27.0
16	54.0
17	125.0
18	318.0
19	581.0
20	1001.0
21	1552.0
22	2559.0
23	4082.0
24	6868.0
25	12695.0
26	21177.0
27	26280.0
28	24348.0
29	19631.0
30	15528.0
31	13587.0
32	13330.0
33	15668.0
34	21951.0
35	32134.0
36	36485.0
37	44586.0
38	75737.0
39	44215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.994196455281408	10.949185616781955	13.662032115095199	44.394585812841434
2	55.81689149074241	22.38184654893893	12.65527128464983	9.145990675668834
3	17.21365420814521	21.366801515088756	52.54002881061851	8.879515466147524
4	16.341281566266723	13.334806080605299	59.903304936925	10.420607416202982
5	19.373300012426306	15.921318476995229	51.98935940096005	12.716022109618422
6	12.67644202668434	25.452984844510105	53.701428104620284	8.169145024185271
7	40.62504314689273	4.533990546803448	51.21984895135792	3.6211173549458997
8	38.14852656237775	9.848767264510013	47.17577698924435	4.826929183867894
9	34.79595546780436	6.076463197426374	47.579401788467464	11.548179546301794
10	20.809688836115445	15.160552464320395	52.77175638919187	11.258002310372282
11	16.77206014331672	14.801110083256244	55.39025501539481	13.036574758032227
12	14.91708893092355	12.93049093109844	58.71360128129013	13.438818856687885
13	13.63050611880468	14.952987145677717	61.54380732783814	9.872699407679457
14	11.156750935424634	19.46787800129786	56.089579852817316	13.285791210460188
15	9.550535941936939	15.267786875060404	63.495197463192824	11.686479719809832
16	10.010999581187495	17.104348746554003	56.478477179320784	16.406174492937716
17	10.135262632259609	16.165012127153318	58.81255148862533	14.887173751961747
18	10.040224409865566	16.829819450389127	59.3669027664637	13.763053373281602
19	10.949185616781955	17.79815078170664	56.709744524371665	14.542919077139741
20	11.288377722856577	18.02504590829387	59.74291355433748	10.943662814512084
21	10.710554535371248	20.633649513763284	57.10393453638376	11.551861414481708
22	9.653628250974544	16.54953723519314	56.25940602261588	17.537428491216442
23	10.223167235055067	19.5953626870274	57.42862928649997	12.752840791417567
24	12.072155411655874	16.525144858501204	55.14817218256544	16.25452754727749
25	9.575388552151361	22.376093629907814	54.35818134121253	13.690336476728293
26	9.981314518986933	17.859591956958962	56.875198475706576	15.283895048347532
27	12.866518471472425	17.99213921143588	54.73074037766763	14.410601939424064
28	9.617269802697889	17.688615203354182	57.96687239105122	14.727242602896709
29	12.82118546950723	18.157132929248302	54.59589195557826	14.425789645666212
30	12.206083366700264	19.013857631362153	56.32521941633185	12.454839585605736
31	15.289187733856158	17.946345975948198	51.583203317363235	15.181262972832416
32	13.746715083233232	19.96930242404996	53.54932092543757	12.734661567279238
33	14.909264961041233	17.79032681182432	49.69187365669341	17.60853457044104
34	14.576286007520215	21.8256543370106	46.8849094030311	16.713150252438087
35	13.416727647608395	21.017254154758124	49.261325196404655	16.304693001228824
36	16.798983804382345	23.51724264892006	45.58129795058012	14.10247559611747
37	14.761760117083409	21.74695440466493	49.136141678287565	14.355143799964102
38	18.676046225854996	22.22513703453132	43.45732024429195	15.641496495321727
39	17.848776469180464	20.827637943492526	44.32900253588671	16.9945830514403
40	17.063848196574945	21.401319029275452	46.00080080632913	15.534031967820471
41	14.668792945540568	23.37687142456082	43.86370644465001	18.090629185248595
42	15.024553458424805	22.02838720366714	48.00879966495	14.93825967295806
43	17.68700438602547	20.894832037775966	44.751036676009406	16.667126900189157
44	16.89126062564145	21.338036919933174	42.80332840883464	18.96737404559073
45	16.016586816150514	20.196197550637194	43.54798624822235	20.239229384989944
46	18.466639973122362	22.163005508995266	43.48102227070015	15.88933224718222
47	13.677219821337347	19.596513270833622	50.38659615889102	16.339670748938012
48	15.446587598547504	20.66011294130642	45.419986100947625	18.473313359198457
49	14.971166369816045	18.318905012403295	49.25902402879221	17.450904588988454
50	14.87060534515213	17.858671489913984	47.15161472931365	20.119108435620234
51	15.08024171464601	17.865114759228835	43.743815612041544	23.310827914083607
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	628.0
1	1179.5
2	1731.0
3	31687.0
4	61643.0
5	39559.0
6	17475.0
7	16508.0
8	15541.0
9	14808.0
10	14075.0
11	13398.5
12	12722.0
13	11871.5
14	11021.0
15	9941.5
16	8862.0
17	8196.5
18	7531.0
19	6736.0
20	5941.0
21	5446.5
22	4952.0
23	4646.0
24	4340.0
25	4707.0
26	4980.5
27	4887.0
28	5652.5
29	6418.0
30	6737.0
31	7056.0
32	7218.0
33	7380.0
34	7447.5
35	7515.0
36	7803.0
37	8091.0
38	9434.5
39	10778.0
40	12431.5
41	14085.0
42	15569.0
43	17053.0
44	36688.5
45	56324.0
46	43993.0
47	31662.0
48	30616.5
49	29571.0
50	24769.5
51	19968.0
52	16828.0
53	13688.0
54	11420.0
55	9152.0
56	7580.5
57	6009.0
58	5099.5
59	4190.0
60	3562.0
61	2934.0
62	2571.0
63	2208.0
64	1815.5
65	1423.0
66	1141.5
67	860.0
68	717.0
69	574.0
70	508.5
71	443.0
72	375.0
73	307.0
74	260.0
75	167.5
76	122.0
77	86.0
78	50.0
79	47.5
80	45.0
81	27.5
82	10.0
83	8.0
84	6.0
85	4.0
86	2.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	434562.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.81051753084869
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.65315484547368	36.58920126652201
2	8.830953374597167	7.914391820129875
3	3.120636386467062	4.195119945095598
4	1.531659832695359	2.7453787913716865
5	0.9941102859138023	2.2273298197268723
6	0.6393035517671292	1.7188513808396866
7	0.5297882612735677	1.6618060318605936
8	0.401825264311739	1.440479844062327
9	0.33577984449857534	1.3541821747568152
>10	1.804203564291932	13.528447275673457
>50	0.10002243209487754	3.1334919369978205
>100	0.04819733774519989	3.9904240682897356
>500	0.004664258491470957	1.3481005480080264
>1k	0.0036277566044774106	3.3027882848871917
>5k	0.001554752830490319	5.51641350859305
>10k+	5.18250943496773E-4	9.333593303185287
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40191	9.24862275118395	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCG	9179	2.112241751464693	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTC	7962	1.8321896530299475	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGC	6613	1.521762142110907	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCT	2657	0.6114202346270498	No Hit
GCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	2345	0.5396238051187172	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCC	2271	0.5225951647866127	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTA	2132	0.4906089349736056	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTC	2052	0.47219959407403317	No Hit
GAACTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCT	1757	0.4043151495068598	No Hit
CTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGCT	1008	0.2319576953346128	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGC	934	0.21492905500250825	No Hit
CCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	768	0.17672967263589545	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATGTGCTTT	698	0.16062149934876957	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCATGTGCTTTCGT	610	0.14037122435923988	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	603	0.13876040703052728	No Hit
GAATGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTT	593	0.13645923941808075	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	572	0.13162678743194298	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCG	523	0.12035106613095484	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	504	0.1159788476673064	No Hit
TCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTATGCCGTCTTCTGC	498	0.11459814709983845	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	490	0.11275721300988123	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCATGTGCTTTCGTAT	485	0.11160662920365794	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	465	0.10700429397876483	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3011676124465555E-4	0.0	0.0	0.247835751860494	0.0
2	2.3011676124465555E-4	0.0	0.0	1.2067322959669737	0.0
3	2.3011676124465555E-4	0.0	0.0	1.5604217580000093	0.0
4	2.3011676124465555E-4	0.0	0.0	2.382398829165919	0.0
5	2.3011676124465555E-4	0.0	0.0	5.299358894703173	0.0
6	2.3011676124465555E-4	0.0	0.0	6.055752688914355	0.0
7	2.3011676124465555E-4	0.0	0.0	7.302295184576654	0.0
8	2.3011676124465555E-4	0.0	0.0	8.685296919657034	0.0
9	2.3011676124465555E-4	0.0	0.0	9.103419074838573	0.0
10	2.3011676124465555E-4	0.0	0.0	12.22564329140606	0.0
11	2.3011676124465555E-4	0.0	0.0	14.000073637363599	0.0
12	2.3011676124465555E-4	0.0	0.0	16.834191668852775	0.0
13	2.3011676124465555E-4	0.0	0.0	17.465862178469354	0.0
14	2.3011676124465555E-4	0.0	0.0	17.778820973762087	0.0
15	2.3011676124465555E-4	0.0	0.0	18.640838361384567	0.0
16	2.3011676124465555E-4	0.0	0.0	19.481224773450048	0.0
17	2.3011676124465555E-4	0.0	0.0	20.32414246988922	0.0
18	2.3011676124465555E-4	0.0	0.0	21.251052784182694	0.0
19	2.3011676124465555E-4	0.0	0.0	21.900442284415114	0.0
20	2.3011676124465555E-4	0.0	0.0	22.408770210004555	0.0
21	2.3011676124465555E-4	0.0	0.0	22.974167092382675	0.0
22	2.3011676124465555E-4	0.0	0.0	23.56855868667762	0.0
23	2.3011676124465555E-4	0.0	0.0	24.12314008127724	0.0
24	2.3011676124465555E-4	0.0	0.0	24.58176278643784	0.0
25	2.3011676124465555E-4	0.0	0.0	24.992751322020794	0.0
26	2.3011676124465555E-4	0.0	0.0	25.357716505354816	0.0
27	2.3011676124465555E-4	0.0	0.0	25.73142612561614	0.0
28	2.3011676124465555E-4	0.0	0.0	26.122394502970806	0.0
29	2.3011676124465555E-4	0.0	0.0	26.489890970678523	0.0
30	2.3011676124465555E-4	0.0	0.0	26.915146745458646	0.0
31	2.3011676124465555E-4	0.0	0.0	27.31025722451572	0.0
32	2.3011676124465555E-4	0.0	0.0	27.704217119766568	0.0
33	2.3011676124465555E-4	0.0	0.0	28.09288432950879	0.0
34	2.3011676124465555E-4	0.0	0.0	28.47027581795003	0.0
35	2.3011676124465555E-4	0.0	0.0	28.843295087927615	0.0
36	2.3011676124465555E-4	0.0	0.0	29.20526875336546	0.0
37	2.3011676124465555E-4	0.0	0.0	29.552514946083644	0.0
38	2.3011676124465555E-4	0.0	0.0	29.91771024617891	0.0
39	2.3011676124465555E-4	0.0	0.0	30.258973403104736	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGGC	30	2.1623728E-6	45.000004	4
CGGGTGA	20	7.02844E-4	45.0	6
CCGGGCG	20	7.02844E-4	45.0	5
CCCTCGT	40	6.7975634E-9	45.0	14
TTGGACG	25	3.886788E-5	45.0	1
GGATCAC	20	7.02844E-4	45.0	8
TACGGGA	25	3.886788E-5	45.0	4
TAACGCT	20	7.02844E-4	45.0	36
TGTGCGG	20	7.02844E-4	45.0	2
AATGCGG	25	3.886788E-5	45.0	2
GCCCTCG	45	3.8380676E-10	45.0	13
GCGATCG	25	3.886788E-5	45.0	9
ACGCTAA	20	7.02844E-4	45.0	38
GTATCCG	20	7.02844E-4	45.0	9
TAGTGCG	25	3.886788E-5	45.0	1
AGCTACG	35	1.2095916E-7	45.0	9
TTCGATG	25	3.886788E-5	45.0	1
TGCGTAG	25	3.886788E-5	45.0	1
CGTTTTT	16565	0.0	44.320858	1
TACGGCT	725	0.0	42.206898	7
>>END_MODULE