##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552166_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	551590
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.488904802480103	31.0	30.0	33.0	28.0	34.0
2	30.534105041788283	31.0	30.0	33.0	25.0	34.0
3	30.012012545550135	31.0	30.0	34.0	25.0	34.0
4	34.46786018600773	35.0	33.0	37.0	32.0	37.0
5	35.12801537373774	35.0	35.0	37.0	33.0	37.0
6	35.05667978027158	35.0	35.0	37.0	32.0	37.0
7	35.60032451639805	37.0	35.0	37.0	35.0	37.0
8	35.23709276817927	37.0	35.0	37.0	32.0	37.0
9	37.28293478852046	39.0	37.0	39.0	34.0	39.0
10	36.73076560488769	39.0	35.0	39.0	32.0	39.0
11	36.92055874834569	39.0	37.0	39.0	33.0	39.0
12	37.17376855998115	39.0	37.0	39.0	34.0	39.0
13	37.12106818470241	39.0	37.0	39.0	33.0	39.0
14	38.17207527330082	40.0	38.0	41.0	33.0	41.0
15	38.36164905092551	40.0	38.0	41.0	34.0	41.0
16	38.1609691981363	40.0	37.0	41.0	34.0	41.0
17	37.947587882303885	40.0	37.0	41.0	33.0	41.0
18	37.736441922442395	39.0	37.0	40.0	33.0	41.0
19	37.12978299098968	38.0	36.0	40.0	32.0	41.0
20	36.88734386047608	38.0	35.0	40.0	32.0	41.0
21	37.0604271288457	39.0	35.0	40.0	33.0	41.0
22	37.3977320110952	39.0	35.0	40.0	33.0	41.0
23	37.466979096792905	39.0	35.0	41.0	33.0	41.0
24	37.4128591888903	39.0	35.0	41.0	33.0	41.0
25	36.958253412861005	39.0	35.0	40.0	33.0	41.0
26	37.09366921082688	39.0	35.0	40.0	33.0	41.0
27	37.322843053717435	39.0	35.0	41.0	33.0	41.0
28	37.20448884134955	39.0	35.0	41.0	33.0	41.0
29	37.1459109120905	39.0	35.0	41.0	33.0	41.0
30	36.80891966859443	39.0	35.0	41.0	32.0	41.0
31	36.69237839699777	39.0	35.0	41.0	32.0	41.0
32	36.33438060878551	39.0	35.0	41.0	31.0	41.0
33	35.86415272213057	39.0	35.0	41.0	27.0	41.0
34	35.41570369296035	39.0	35.0	41.0	23.0	41.0
35	35.179736761000015	39.0	35.0	41.0	20.0	41.0
36	34.95821352816404	39.0	35.0	41.0	18.0	41.0
37	34.633849417139544	39.0	35.0	41.0	16.0	41.0
38	34.639476785293425	39.0	35.0	41.0	18.0	41.0
39	34.55909099149731	39.0	35.0	41.0	16.0	41.0
40	34.43842165376457	39.0	35.0	41.0	15.0	41.0
41	34.24989756884643	39.0	34.0	41.0	15.0	41.0
42	34.15439728784061	39.0	34.0	41.0	15.0	41.0
43	34.23426095469461	39.0	34.0	41.0	15.0	41.0
44	34.207659674758425	39.0	34.0	41.0	15.0	41.0
45	34.182628401530124	39.0	34.0	41.0	15.0	41.0
46	34.09047843506953	38.0	34.0	40.0	15.0	41.0
47	34.04436991243496	38.0	34.0	40.0	15.0	41.0
48	34.0399572145978	38.0	34.0	40.0	15.0	41.0
49	34.00994579307094	38.0	34.0	40.0	15.0	41.0
50	33.92682245871028	38.0	34.0	40.0	13.0	41.0
51	32.64495005348175	37.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	15.0
10	22.0
11	14.0
12	11.0
13	19.0
14	22.0
15	37.0
16	68.0
17	118.0
18	315.0
19	539.0
20	878.0
21	1306.0
22	2022.0
23	3082.0
24	5048.0
25	8912.0
26	13263.0
27	15821.0
28	14954.0
29	12872.0
30	11994.0
31	12592.0
32	14922.0
33	20148.0
34	30469.0
35	38258.0
36	46948.0
37	71119.0
38	126731.0
39	99060.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.88777896626117	16.815388241266156	18.91839953588716	24.37843325658551
2	41.79589912797549	24.164324951503836	20.212839246541815	13.826936673978862
3	25.423593611196722	24.540872749687267	36.98943055530376	13.046103083812252
4	22.5783643648362	21.356079696876304	40.89504885875378	15.170507079533714
5	23.15778023531971	24.74772929168404	35.29306187566853	16.801428597327725
6	19.64538878514839	32.15141681321271	36.576805235772945	11.626389165865952
7	63.67610000181294	5.599267571928425	26.807230007795646	3.9174024184629888
8	63.08381225185373	8.497978571040084	23.841621494225784	4.5765876828804
9	57.7189579216447	7.685781105531282	25.161623669754707	9.43363730306931
10	28.726046520060187	27.615620297684874	31.935676861436935	11.722656320817999
11	21.322177704454397	22.034844721622946	41.42279591725738	15.220181656665277
12	20.320709222429702	19.77374499175112	43.79919868017912	16.106347105640058
13	18.70773581827082	22.00203049366377	45.423231023042476	13.867002665022934
14	15.795971645606338	25.59890498377418	41.97302344132417	16.63209992929531
15	14.438985478344424	23.25912362443119	47.04490654290324	15.256984354321142
16	16.260447071194186	24.272920103700212	41.300241121122575	18.16639170398303
17	16.51897242517087	22.89508511756921	42.14742834351602	18.438514113743903
18	16.77804166137892	22.60646494679019	43.99535887162566	16.620134520205223
19	16.52785583494987	24.199858590619844	41.356442285030546	17.915843289399735
20	16.98435432114433	24.437897713881686	43.83872078899182	14.73902717598216
21	16.44065338385395	26.17813956018057	42.20816185935205	15.173045196613428
22	15.4234123171196	22.37658405699886	42.435323337986546	19.764680287894993
23	14.642941315107235	25.743033775086566	42.52615167062493	17.087873239181274
24	15.9237839699777	23.465980166427965	41.73045196613427	18.87978389746007
25	14.772747874326948	27.8059790786635	39.879802026867786	17.54147102014177
26	14.46110335575337	24.757337877771533	41.412099566707155	19.369459199767945
27	16.2718685980529	24.782175166337318	40.09064703856125	18.855309197048534
28	13.141282474301564	24.439710654652913	42.71524139306369	19.703765477981836
29	16.00137783498613	24.167406950814918	40.04441704889501	19.78679816530394
30	16.449174205478705	24.64819884334379	40.16316466941025	18.739462281767256
31	16.71060026468935	24.36610525934118	38.25631356623579	20.666980909733677
32	17.34857412208343	26.37973857394079	36.41164633151435	19.86004097246143
33	17.856197538026432	24.674849072680797	35.83059881433673	21.638354574956036
34	16.497398429993293	26.766257546365964	37.47439221160644	19.2619518120343
35	15.555756993419026	28.405881179861854	34.50787722765097	21.53048459906815
36	17.267354375532552	29.573052448376515	33.215613045921785	19.943980130169148
37	15.726717308145544	27.506481263257132	35.66652767454087	21.100273754056456
38	17.24704943889483	27.92944034518392	33.18171105349988	21.641799162421364
39	18.145724179191067	26.442647618702296	35.574248989285515	19.83737921282112
40	19.214271469751083	27.16800522126942	35.54361029025181	18.07411301872768
41	16.27621965590384	29.406261897423814	34.699505067169454	19.61801337950289
42	16.612157580811836	28.11073442230642	35.67740531916822	19.59970267771352
43	18.232020159901378	26.654217806704256	33.90743124422125	21.206330789173116
44	17.729291684040682	25.496473830199967	34.800123279972446	21.974111205786905
45	17.208796388621984	25.52203629507424	34.10612955274751	23.163037763556265
46	19.156438659149007	27.276056491234428	34.1721205968201	19.39538425279646
47	14.998096412190215	26.148044743378236	39.339364382965606	19.514494461465944
48	16.841132000217556	26.46168349680016	35.62392356641709	21.0732609365652
49	17.248137203357565	24.94062618974238	38.10076324806468	19.71047335883537
50	17.29400460486956	24.87536032197828	36.32553164488116	21.505103428271
51	16.22745154915789	24.409434543773457	34.26131728276437	25.101796624304285
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	624.0
1	1338.0
2	2052.0
3	16801.5
4	31551.0
5	20292.0
6	9033.0
7	8556.5
8	8080.0
9	7999.5
10	7919.0
11	7572.5
12	7226.0
13	6864.5
14	6503.0
15	6024.0
16	5545.0
17	5379.0
18	5213.0
19	4997.0
20	4781.0
21	5020.0
22	5259.0
23	5120.5
24	4982.0
25	5645.0
26	7424.5
27	8541.0
28	9395.5
29	10250.0
30	11730.0
31	13210.0
32	15106.5
33	17003.0
34	18797.5
35	20592.0
36	21466.5
37	22341.0
38	23679.0
39	25017.0
40	26990.0
41	28963.0
42	30508.5
43	32054.0
44	33992.5
45	35931.0
46	50492.0
47	65053.0
48	54882.0
49	44711.0
50	42065.5
51	39420.0
52	33467.5
53	27515.0
54	23307.5
55	19100.0
56	15586.0
57	12072.0
58	10109.0
59	8146.0
60	6882.5
61	5619.0
62	4747.0
63	3875.0
64	3201.5
65	2528.0
66	2057.5
67	1587.0
68	1342.0
69	1097.0
70	877.0
71	657.0
72	559.5
73	462.0
74	418.5
75	284.0
76	193.0
77	138.5
78	84.0
79	69.5
80	55.0
81	37.5
82	20.0
83	21.5
84	23.0
85	18.0
86	13.0
87	9.0
88	5.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	551590.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.341714876808375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.68471619673271	30.56249954897636
2	8.997916818005082	7.079869558783897
3	3.3694249548730606	3.9767686762025662
4	1.903329412819568	2.9952097230316213
5	1.2262685165948322	2.4121753171140323
6	0.8769322025787828	2.070001000808762
7	0.6635003743366152	1.827226978346472
8	0.5444701441552451	1.7136311336232324
9	0.4656506736773396	1.6487546432406872
>10	4.146919168642652	31.41738663995622
>50	0.08198650805139264	2.072976231137066
>100	0.031857614556924325	2.312215419177519
>500	0.002342471658597377	0.6845410973954011
>1k	0.0028109659903168523	2.0147331006001963
>5k	0.0014054829951584261	3.506568222387589
>10k+	4.684943317194754E-4	3.705442709218402
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20104	3.644736126470748	No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTC	6843	1.2405953697492704	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCG	6831	1.2384198408238003	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGC	5351	0.9701046066824996	No Hit
GCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	2505	0.4541416631918635	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCC	2081	0.3772729744919233	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCT	1754	0.31798981127286574	No Hit
GAACTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCT	1661	0.3011294621004732	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCCGTAATCGTA	1504	0.2726662919922406	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTC	1426	0.2585253539766856	No Hit
CTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGCT	929	0.16842219764680286	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGC	839	0.15210573070577785	No Hit
CCTGTCTCTTATACACATCTGACGCACCCGTAATCGTATGCCGTCTTCTGC	712	0.1290813829112203	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	652	0.11820373828387028	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCCGTAAT	582	0.10551315288529522	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8129407712250042E-4	0.0	0.0	0.1829257238166029	0.0
2	1.8129407712250042E-4	0.0	0.0	0.9456299062709621	0.0
3	1.8129407712250042E-4	0.0	0.0	1.1978099675483602	0.0
4	1.8129407712250042E-4	0.0	0.0	1.7964430102068565	0.0
5	1.8129407712250042E-4	0.0	0.0	3.873891839953589	0.0
6	1.8129407712250042E-4	0.0	0.0	4.477419822694393	0.0
7	1.8129407712250042E-4	0.0	0.0	5.3218876339309995	0.0
8	1.8129407712250042E-4	0.0	0.0	6.3967802171903045	0.0
9	1.8129407712250042E-4	0.0	0.0	6.7898257763918854	0.0
10	1.8129407712250042E-4	0.0	0.0	8.89863848148081	0.0
11	1.8129407712250042E-4	0.0	0.0	10.259069236208052	0.0
12	1.8129407712250042E-4	0.0	0.0	12.313856306314472	0.0
13	1.8129407712250042E-4	0.0	0.0	12.785583494987218	0.0
14	1.8129407712250042E-4	0.0	0.0	13.013107561775957	0.0
15	1.8129407712250042E-4	0.0	0.0	13.587809786254283	0.0
16	1.8129407712250042E-4	0.0	0.0	14.198408238002864	0.0
17	1.8129407712250042E-4	0.0	0.0	14.87028408781885	0.0
18	1.8129407712250042E-4	0.0	0.0	15.605068982396345	0.0
19	1.8129407712250042E-4	0.0	0.0	16.1340850994398	0.0
20	1.8129407712250042E-4	0.0	0.0	16.578255588389926	0.0
21	1.8129407712250042E-4	0.0	0.0	17.0896861799525	0.0
22	1.8129407712250042E-4	0.0	0.0	17.643358291484617	0.0
23	1.8129407712250042E-4	0.0	0.0	18.149168766656395	0.0
24	1.8129407712250042E-4	0.0	0.0	18.558893380953243	0.0
25	1.8129407712250042E-4	0.0	0.0	18.938885766602006	0.0
26	1.8129407712250042E-4	0.0	0.0	19.316521329248175	0.0
27	1.8129407712250042E-4	0.0	0.0	19.729327942856106	0.0
28	1.8129407712250042E-4	0.0	0.0	20.09862397795464	0.0
29	1.8129407712250042E-4	0.0	0.0	20.508529886328613	0.0
30	1.8129407712250042E-4	0.0	0.0	20.9846081328523	0.0
31	3.6258815424500084E-4	0.0	0.0	21.39868380500009	0.0
32	3.6258815424500084E-4	0.0	0.0	21.786109247810874	0.0
33	3.6258815424500084E-4	0.0	0.0	22.15486140067804	0.0
34	3.6258815424500084E-4	0.0	0.0	22.543012019797313	0.0
35	3.6258815424500084E-4	0.0	0.0	22.959625809024818	0.0
36	3.6258815424500084E-4	0.0	0.0	23.349589368915318	0.0
37	3.6258815424500084E-4	0.0	0.0	23.73683351764898	0.0
38	3.6258815424500084E-4	0.0	0.0	24.156166718033322	0.0
39	3.6258815424500084E-4	0.0	0.0	24.754437172537575	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAC	30	2.1634405E-6	45.000004	26
GGGTCCG	30	2.1634405E-6	45.000004	7
TCGGGGC	30	2.1634405E-6	45.000004	5
TCGATTG	20	7.03009E-4	45.000004	1
CGCGACC	20	7.03009E-4	45.000004	10
TATTGCG	20	7.03009E-4	45.000004	1
CGCTCTA	20	7.03009E-4	45.000004	28
TACGCGG	20	7.03009E-4	45.000004	2
GCTACGA	120	0.0	45.000004	10
GTAAGCG	20	7.03009E-4	45.000004	1
AGGTGCG	20	7.03009E-4	45.000004	1
ACGACCA	30	2.1634405E-6	45.000004	28
CGACGGT	25	3.8881582E-5	45.0	28
TGGTTCG	25	3.8881582E-5	45.0	1
TTGAGCG	25	3.8881582E-5	45.0	1
TCGTGCG	35	1.2103919E-7	45.0	1
CATAGCG	35	1.2103919E-7	45.0	1
GCGATCA	25	3.8881582E-5	45.0	9
CGGTCTA	25	3.8881582E-5	45.0	31
GTGCGAG	25	3.8881582E-5	45.0	1
>>END_MODULE