SRR3552163_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552163_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	628034
Sequences flagged as poor quality	0
Sequence length	51
%GC	35

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38336	6.104128120452077	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG	11601	1.84719298636698	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	9481	1.5096316441466544	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGC	8842	1.4078855603359053	No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	3772	0.6006044258750323	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCT	2932	0.4668537053726391	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAGTTACT	2835	0.451408681695577	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTA	2692	0.4286392138005267	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCC	2397	0.381667234576472	No Hit
GAACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT	2053	0.32689312998977765	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTC	1909	0.3039644350465102	No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	1245	0.19823767503033274	TruSeq Adapter, Index 27 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	1181	0.1880471439444361	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1080	0.17196521207450552	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGC	987	0.157157096590312	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	758	0.12069410254858814	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGT	748	0.11910183206641678	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	728	0.11591729110207408	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	685	0.10907052802873729	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	679	0.1081151657394345	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGAGTTAC	648	0.10317912724470331	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAC	20	7.0308393E-4	45.000004	23
CGGCGAA	20	7.0308393E-4	45.000004	31
GCGAACC	20	7.0308393E-4	45.000004	33
CAATTCG	20	7.0308393E-4	45.000004	24
CCGCTCC	20	7.0308393E-4	45.000004	35
GCGTTAG	20	7.0308393E-4	45.000004	1
TCGGCGT	20	7.0308393E-4	45.000004	4
ACGATAG	20	7.0308393E-4	45.000004	1
CGCTCCT	20	7.0308393E-4	45.000004	36
GACGGTA	20	7.0308393E-4	45.000004	4
TCCGTAA	25	3.8887767E-5	45.000004	22
CGGTCTA	25	3.8887767E-5	45.000004	31
CGGTAGC	25	3.8887767E-5	45.000004	12
CCTATCG	20	7.0308393E-4	45.000004	11
GGCGAAC	20	7.0308393E-4	45.000004	32
TCGCGAG	20	7.0308393E-4	45.000004	1
GTACGTA	25	3.8887767E-5	45.000004	39
AACACGT	20	7.0308393E-4	45.000004	41
CGGACGG	50	2.1827873E-11	45.000004	2
CGTTTGG	30	2.1639225E-6	44.999996	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content