##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552163_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	628034
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.11740447173242	31.0	28.0	33.0	25.0	33.0
2	28.827272090364534	31.0	28.0	33.0	16.0	34.0
3	28.66929815901687	31.0	27.0	33.0	16.0	34.0
4	33.452322644952346	35.0	32.0	35.0	28.0	37.0
5	34.473553979561615	35.0	35.0	35.0	32.0	37.0
6	33.63250397271485	35.0	33.0	37.0	28.0	37.0
7	34.76689478595108	35.0	35.0	37.0	32.0	37.0
8	33.9999554164265	35.0	35.0	37.0	29.0	37.0
9	36.34774231968333	39.0	35.0	39.0	32.0	39.0
10	36.237573125021896	37.0	35.0	39.0	32.0	39.0
11	36.74718406965228	39.0	35.0	39.0	32.0	39.0
12	36.82393787597487	39.0	35.0	39.0	33.0	39.0
13	36.82578650200467	39.0	37.0	39.0	33.0	39.0
14	37.64221045357417	39.0	37.0	41.0	32.0	41.0
15	37.93695086571746	39.0	37.0	41.0	33.0	41.0
16	37.47546470414022	39.0	36.0	41.0	32.0	41.0
17	37.359470984055	39.0	36.0	41.0	32.0	41.0
18	37.02958438555874	39.0	36.0	39.0	32.0	41.0
19	36.2723944881965	37.0	35.0	39.0	31.0	41.0
20	36.034287952563076	37.0	35.0	39.0	32.0	41.0
21	36.29048586541493	37.0	35.0	39.0	32.0	41.0
22	36.72045621733855	38.0	35.0	40.0	33.0	41.0
23	36.91517656687377	38.0	35.0	40.0	33.0	41.0
24	36.80705025524096	38.0	35.0	40.0	33.0	41.0
25	36.10840495896719	37.0	35.0	40.0	31.0	41.0
26	36.394612393596525	37.0	35.0	40.0	32.0	41.0
27	36.59214310053277	38.0	35.0	40.0	33.0	41.0
28	36.41859517159899	38.0	35.0	40.0	33.0	41.0
29	36.3549250518284	38.0	35.0	40.0	32.0	41.0
30	35.738292831279836	37.0	35.0	40.0	30.0	41.0
31	35.59418916810236	38.0	35.0	40.0	30.0	41.0
32	35.01090864507336	38.0	35.0	40.0	24.0	41.0
33	34.29403662859017	38.0	34.0	40.0	18.0	41.0
34	33.7549256887366	38.0	34.0	40.0	15.0	41.0
35	33.37836327332597	38.0	33.0	40.0	12.0	41.0
36	33.04682867488066	38.0	33.0	40.0	10.0	41.0
37	32.99161032682944	38.0	33.0	40.0	10.0	41.0
38	32.67513064579306	38.0	32.0	40.0	10.0	41.0
39	32.5990089708519	38.0	32.0	40.0	10.0	41.0
40	32.396218994513035	37.0	31.0	40.0	10.0	41.0
41	32.27147574812829	37.0	31.0	40.0	10.0	41.0
42	32.39212685937385	38.0	31.0	40.0	8.0	41.0
43	32.37131906871284	38.0	31.0	40.0	8.0	41.0
44	32.36840043691902	38.0	31.0	40.0	8.0	41.0
45	32.37397816041807	38.0	31.0	40.0	8.0	41.0
46	32.20593471054115	37.0	31.0	40.0	8.0	41.0
47	32.03921762197588	37.0	30.0	40.0	8.0	41.0
48	32.114759710461534	37.0	31.0	40.0	8.0	41.0
49	32.13945104882857	37.0	31.0	40.0	8.0	41.0
50	32.06812051576826	36.0	31.0	40.0	8.0	41.0
51	31.20634710859603	35.0	28.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	23.0
11	12.0
12	15.0
13	18.0
14	27.0
15	35.0
16	87.0
17	166.0
18	375.0
19	830.0
20	1375.0
21	2269.0
22	3248.0
23	4867.0
24	7746.0
25	13738.0
26	21594.0
27	26189.0
28	24789.0
29	20965.0
30	18825.0
31	19408.0
32	22508.0
33	28736.0
34	37676.0
35	54337.0
36	70561.0
37	102464.0
38	114202.0
39	30940.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.80278456261922	14.371355690933928	16.65578615170516	32.17007359474169
2	47.79375001990338	24.599145906113364	15.73736453758873	11.869739536394526
3	22.761824996735847	22.8710547518128	42.55836467452399	11.808755576927364
4	19.896693491116725	17.693787279032662	49.29987866898926	13.109640560861354
5	22.88379291567017	20.752857329380255	41.77687832187429	14.58647143307528
6	16.75036701834614	30.632417990108816	42.43161994414315	10.185595047401891
7	51.41632459389142	5.700328326173424	39.09963473315139	3.7837123467837728
8	49.80239923316254	10.757697831646057	34.14369285739307	5.296210077798335
9	44.57099456398858	8.151628733476212	35.18137553062414	12.096001171911075
10	26.4775473939309	19.44894703153014	41.62035813347685	12.453147441062109
11	21.666502132050176	18.985596321218278	45.058070104484784	14.289831442246758
12	18.378304359318125	17.35208603355869	48.5763509618906	15.693258645232582
13	17.354792893378384	19.312967132352707	51.29674508068035	12.035494893588563
14	14.038093479015467	23.70906670657958	46.911154491635806	15.34168532276915
15	12.952483464271042	19.468691185509066	53.581493995548016	13.99733135467188
16	13.524745475563424	20.69553559202209	47.057484148947346	18.722234783467137
17	13.561208469605148	21.242480502647947	48.75595907227952	16.44035195546738
18	13.400866832050495	20.686778104370145	49.61912890066461	16.29322616291475
19	14.834387947149358	21.02449867363869	47.525770897753944	16.615342481458008
20	15.056191225315827	21.68465401554693	50.67050510004235	12.588649659094889
21	14.442848635583424	24.629717499371054	47.492810898772994	13.434622966272528
22	12.932102402099249	20.32692497539942	47.87415967925304	18.866812943248295
23	13.754669333188968	22.806408570236645	48.077014938681664	15.361907157892727
24	14.473101774744679	20.543632988022942	47.00239159026422	17.980873646968156
25	12.656639608683607	25.98951649114538	45.531292891786116	15.822551008384897
26	13.657063152631862	21.087711811780892	48.27764101943525	16.977584016151994
27	16.748137839671102	21.99562444071499	45.55867994407946	15.697557775534445
28	12.141221653604742	22.486362203320205	48.73462264781843	16.637793495256624
29	18.52479324367789	19.8156469235742	45.09023396822465	16.569325864523258
30	16.233356792785102	23.248582083135627	46.40226484553384	14.115796278545428
31	17.26451115703927	23.249855899521364	43.47774165092972	16.00789129250964
32	19.554036883353447	24.32272775040842	41.73945996554327	14.383775400694867
33	15.205546196543498	22.530468095676348	42.51728409608397	19.74670161169618
34	18.333720785817327	23.45796565154116	41.98594343618339	16.22237012645812
35	19.090845400089805	24.801841938493776	38.437568666664546	17.669743994751876
36	18.424002522156442	26.892015400440105	37.38682937547967	17.297152701923782
37	19.862618902798257	26.327873968606795	38.90123783107284	14.908269297522109
38	16.542575720422782	28.3193266606585	37.43603053337877	17.702067085539955
39	20.356063525223156	23.263230971571602	39.87777731778853	16.502928185416714
40	17.11276778008834	25.922959584990622	40.896352745233536	16.067919889687502
41	16.177786552957325	26.90825655935825	37.15388020393801	19.760076683746423
42	16.795587500039808	26.414652709885132	38.84805599696832	17.941703793106743
43	19.746064703503315	23.69298477470965	36.16715018613642	20.393800335650617
44	18.28085740580924	23.856033272083994	38.35047147128977	19.512637850816994
45	17.010862469229373	23.70747443609741	38.56415416999717	20.717508924676054
46	19.61565775101348	25.190355936143582	37.99698742424773	17.196998888595203
47	15.270988513360741	22.53827022103899	44.77337214227255	17.417369123327717
48	16.951630007292597	24.44421798819809	39.43544457784133	19.168707426667982
49	16.587796202116444	22.1462532283284	42.74306805045587	18.522882519099284
50	16.812465567150824	21.65774464439823	40.651143090979154	20.878646697471794
51	16.95417764006407	20.513857530006337	38.575936971565234	23.956027858364358
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	601.0
1	1439.5
2	2278.0
3	31979.5
4	61681.0
5	39586.5
6	17492.0
7	16315.0
8	15138.0
9	14252.0
10	13366.0
11	12734.5
12	12103.0
13	11393.0
14	10683.0
15	9842.5
16	9002.0
17	8152.0
18	7302.0
19	6754.5
20	6207.0
21	6149.0
22	6091.0
23	6038.0
24	5985.0
25	6503.0
26	7733.0
27	8445.0
28	9776.5
29	11108.0
30	12183.0
31	13258.0
32	13778.0
33	14298.0
34	14870.5
35	15443.0
36	16470.0
37	17497.0
38	18711.0
39	19925.0
40	21950.0
41	23975.0
42	26556.5
43	29138.0
44	30766.0
45	32394.0
46	56492.5
47	80591.0
48	66085.5
49	51580.0
50	48758.0
51	45936.0
52	38353.5
53	30771.0
54	25840.0
55	20909.0
56	17222.0
57	13535.0
58	10904.0
59	8273.0
60	7034.5
61	5796.0
62	4829.0
63	3862.0
64	3118.5
65	2375.0
66	1864.5
67	1354.0
68	1136.5
69	919.0
70	779.0
71	639.0
72	550.0
73	461.0
74	361.5
75	222.5
76	183.0
77	131.5
78	80.0
79	58.0
80	36.0
81	29.5
82	23.0
83	16.0
84	9.0
85	7.5
86	6.0
87	3.0
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	628034.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.52120353225027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.77232295301589	31.919493310873065
2	9.765748592783705	7.914397727459503
3	3.5198192654843945	4.278819385602865
4	1.7747642936783659	2.8766234066364578
5	1.0198592367531623	2.066296185336015
6	0.7238461134989127	1.759866941467066
7	0.5311378006230283	1.5065640045902244
8	0.43884202848647935	1.422592572384496
9	0.3607353532278811	1.3155687602482646
>10	2.9058331468740937	21.893687349533096
>50	0.12314864825559046	3.3720173444779844
>100	0.0521013511483915	3.597852778180784
>500	0.00631531529071412	1.624671639064699
>1k	0.003947072056696326	3.5340366742246094
>5k	7.89414411339265E-4	2.9305826385688594
>10k+	7.89414411339265E-4	7.986929281352024
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38336	6.104128120452077	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG	11601	1.84719298636698	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	9481	1.5096316441466544	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGC	8842	1.4078855603359053	No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	3772	0.6006044258750323	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCT	2932	0.4668537053726391	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAGTTACT	2835	0.451408681695577	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTA	2692	0.4286392138005267	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCC	2397	0.381667234576472	No Hit
GAACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT	2053	0.32689312998977765	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTC	1909	0.3039644350465102	No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	1245	0.19823767503033274	TruSeq Adapter, Index 27 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	1181	0.1880471439444361	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1080	0.17196521207450552	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGC	987	0.157157096590312	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	758	0.12069410254858814	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGT	748	0.11910183206641678	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	728	0.11591729110207408	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	685	0.10907052802873729	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	679	0.1081151657394345	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGAGTTAC	648	0.10317912724470331	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.20635825448940662	0.0
2	0.0	0.0	0.0	1.1942028616285105	0.0
3	0.0	0.0	0.0	1.451354544499183	0.0
4	0.0	0.0	0.0	2.1080068913466468	0.0
5	0.0	0.0	0.0	4.4960941605072335	0.0
6	0.0	0.0	0.0	5.087622644633889	0.0
7	0.0	0.0	0.0	6.087409280389278	0.0
8	0.0	0.0	0.0	7.167924029590755	0.0
9	0.0	0.0	0.0	7.550228172360095	0.0
10	0.0	0.0	0.0	10.366954655321209	0.0
11	0.0	0.0	0.0	11.903654897664776	0.0
12	0.0	0.0	0.0	14.590133655184273	0.0
13	0.0	0.0	0.0	15.171790062321467	0.0
14	0.0	0.0	0.0	15.448048990978196	0.0
15	0.0	0.0	0.0	16.25867389345163	0.0
16	0.0	0.0	0.0	17.105124881773918	0.0
17	0.0	0.0	0.0	17.968135483110785	0.0
18	0.0	0.0	0.0	18.896110720120248	0.0
19	0.0	0.0	0.0	19.9224882729279	0.0
20	0.0	0.0	0.0	20.550161296999843	0.0
21	0.0	0.0	0.0	21.1639815678769	0.0
22	0.0	0.0	0.0	21.85518618418748	0.0
23	0.0	0.0	0.0	22.491775922959587	0.0
24	0.0	0.0	0.0	23.04333841798374	0.0
25	0.0	0.0	0.0	23.51894961100832	0.0
26	0.0	0.0	0.0	23.97019906565568	0.0
27	0.0	0.0	0.0	24.446924848017783	0.0
28	0.0	0.0	0.0	24.919192273029804	0.0
29	0.0	0.0	0.0	25.40069486683842	0.0
30	0.0	0.0	0.0	25.950983545476838	0.0
31	0.0	0.0	0.0	26.434078409767626	0.0
32	0.0	0.0	0.0	26.931026027253303	0.0
33	0.0	0.0	0.0	27.411573258772613	0.0
34	0.0	0.0	0.0	27.90740628692077	0.0
35	0.0	0.0	0.0	28.419480473987075	0.0
36	0.0	0.0	0.0	28.89509166701166	0.0
37	0.0	0.0	0.0	29.3730912657595	0.0
38	0.0	0.0	0.0	29.85252390794129	0.0
39	0.0	0.0	0.0	30.35329297458418	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAC	20	7.0308393E-4	45.000004	23
CGGCGAA	20	7.0308393E-4	45.000004	31
GCGAACC	20	7.0308393E-4	45.000004	33
CAATTCG	20	7.0308393E-4	45.000004	24
CCGCTCC	20	7.0308393E-4	45.000004	35
GCGTTAG	20	7.0308393E-4	45.000004	1
TCGGCGT	20	7.0308393E-4	45.000004	4
ACGATAG	20	7.0308393E-4	45.000004	1
CGCTCCT	20	7.0308393E-4	45.000004	36
GACGGTA	20	7.0308393E-4	45.000004	4
TCCGTAA	25	3.8887767E-5	45.000004	22
CGGTCTA	25	3.8887767E-5	45.000004	31
CGGTAGC	25	3.8887767E-5	45.000004	12
CCTATCG	20	7.0308393E-4	45.000004	11
GGCGAAC	20	7.0308393E-4	45.000004	32
TCGCGAG	20	7.0308393E-4	45.000004	1
GTACGTA	25	3.8887767E-5	45.000004	39
AACACGT	20	7.0308393E-4	45.000004	41
CGGACGG	50	2.1827873E-11	45.000004	2
CGTTTGG	30	2.1639225E-6	44.999996	2
>>END_MODULE