SRR3552162_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552162_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	651395
Sequences flagged as poor quality	0
Sequence length	51
%GC	35

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42612	6.5416529141304425	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG	14222	2.1833142716784746	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	11617	1.7834033113548613	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGC	10455	1.6050169252143476	No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	3936	0.6042416659630486	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCT	3517	0.5399181756077341	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTA	2908	0.4464265154015613	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTC	2362	0.36260640625119933	No Hit
GAACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT	2310	0.3546235387130696	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCC	2288	0.35124617167770705	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1211	0.1859087036283668	No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	1206	0.18514112021123896	TruSeq Adapter, Index 27 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAGTTACT	1181	0.18130320312559967	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	1054	0.16180658433055212	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGC	959	0.14722249940512286	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	842	0.129261047444331	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	784	0.12035707980564787	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	766	0.11759377950398758	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGT	758	0.11636564603658302	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	716	0.10991794533270903	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	683	0.10485189477966517	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTCGT	20	7.031032E-4	45.000004	14
CTAAGCG	20	7.031032E-4	45.000004	1
CGACAAT	30	2.164048E-6	45.000004	20
GCCCTCG	20	7.031032E-4	45.000004	13
TCATCGA	30	2.164048E-6	45.000004	16
TTCATCG	30	2.164048E-6	45.000004	15
TAGTGCG	20	7.031032E-4	45.000004	1
TATACGG	20	7.031032E-4	45.000004	2
TCGACAA	30	2.164048E-6	45.000004	19
GTTATCA	20	7.031032E-4	45.000004	22
AACACGT	20	7.031032E-4	45.000004	41
CTCACGA	25	3.8889382E-5	45.0	24
CGACGGT	25	3.8889382E-5	45.0	28
CCGATGA	35	1.2108467E-7	45.0	18
TCGATGC	45	3.8380676E-10	45.0	24
ATCCGGC	25	3.8889382E-5	45.0	6
CCGTAAT	25	3.8889382E-5	45.0	23
GTTCCGT	25	3.8889382E-5	45.0	20
GCGATCG	25	3.8889382E-5	45.0	9
TCCGTAA	25	3.8889382E-5	45.0	22

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content