##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552162_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	651395
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.69290215614182	31.0	30.0	33.0	28.0	34.0
2	30.677269552268594	31.0	30.0	33.0	26.0	34.0
3	30.330874507787133	31.0	30.0	34.0	25.0	34.0
4	34.501674099432755	35.0	35.0	37.0	32.0	37.0
5	35.20526408707466	35.0	35.0	37.0	33.0	37.0
6	35.13272438382241	36.0	35.0	37.0	33.0	37.0
7	35.636446395812065	37.0	35.0	37.0	35.0	37.0
8	35.07873717176214	37.0	35.0	37.0	32.0	37.0
9	37.14678651202419	39.0	37.0	39.0	33.0	39.0
10	36.85446464894572	39.0	37.0	39.0	32.0	39.0
11	37.121520736266014	39.0	37.0	39.0	34.0	39.0
12	37.33835077026996	39.0	37.0	39.0	34.0	39.0
13	37.25604894111868	39.0	37.0	39.0	34.0	39.0
14	38.4181947973196	40.0	38.0	41.0	34.0	41.0
15	38.50489487945102	40.0	38.0	41.0	34.0	41.0
16	38.35404478081656	40.0	37.0	41.0	34.0	41.0
17	38.06997750980588	40.0	37.0	41.0	33.0	41.0
18	37.68950636710444	39.0	37.0	40.0	33.0	41.0
19	36.961616223643105	37.0	37.0	40.0	33.0	41.0
20	36.43660298282916	37.0	35.0	40.0	32.0	41.0
21	36.63002018744387	37.0	35.0	40.0	33.0	41.0
22	36.92812195365331	38.0	35.0	40.0	33.0	41.0
23	36.99249763968099	38.0	35.0	40.0	33.0	41.0
24	36.873290399834204	38.0	35.0	40.0	33.0	41.0
25	36.43062043767606	37.0	35.0	40.0	33.0	41.0
26	36.597313458040055	38.0	35.0	40.0	33.0	41.0
27	36.79622195442089	38.0	35.0	40.0	33.0	41.0
28	36.62947673838455	38.0	35.0	40.0	33.0	41.0
29	36.54782428480415	38.0	35.0	40.0	33.0	41.0
30	36.06128078968982	38.0	35.0	40.0	31.0	41.0
31	35.80089346709754	38.0	35.0	40.0	30.0	41.0
32	35.23774668212068	38.0	35.0	40.0	25.0	41.0
33	34.34499650749545	38.0	34.0	40.0	18.0	41.0
34	33.74070878652738	38.0	34.0	40.0	15.0	41.0
35	33.31352251705954	38.0	33.0	40.0	10.0	41.0
36	32.95577030833826	38.0	33.0	40.0	10.0	41.0
37	32.62389947727569	38.0	32.0	40.0	9.0	41.0
38	32.38525625772381	37.0	31.0	40.0	9.0	41.0
39	32.380021338819	38.0	31.0	40.0	8.0	41.0
40	32.206948165091845	37.0	31.0	40.0	8.0	41.0
41	32.099426615187404	37.0	30.0	40.0	8.0	41.0
42	31.97313918590103	37.0	30.0	40.0	8.0	41.0
43	31.99904052072859	37.0	30.0	40.0	8.0	41.0
44	31.975943935707214	37.0	30.0	40.0	8.0	41.0
45	31.933148089868666	37.0	29.0	40.0	8.0	41.0
46	31.7851349795439	37.0	29.0	40.0	8.0	41.0
47	31.75378380245473	37.0	29.0	40.0	8.0	41.0
48	31.778174533117387	36.0	29.0	40.0	8.0	41.0
49	31.74192617382694	36.0	29.0	40.0	8.0	41.0
50	31.612169267495144	36.0	29.0	40.0	8.0	41.0
51	30.339583509237865	35.0	25.0	39.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	13.0
10	14.0
11	16.0
12	13.0
13	13.0
14	20.0
15	33.0
16	86.0
17	163.0
18	389.0
19	725.0
20	1169.0
21	1777.0
22	2915.0
23	4602.0
24	7568.0
25	13953.0
26	23017.0
27	28831.0
28	27995.0
29	23144.0
30	19576.0
31	18525.0
32	19686.0
33	24103.0
34	35628.0
35	47800.0
36	57801.0
37	78732.0
38	130493.0
39	82584.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.414924891962634	13.71026796337092	16.240376422907758	33.63443072175868
2	48.568533685398265	25.193162366920223	15.372853644869855	10.865450302811658
3	21.780486494369775	23.45427889375878	43.96579648293278	10.799438128938663
4	19.238250216842314	16.907252895708442	51.34258015489833	12.51191673255091
5	22.374903092593588	20.110071462016137	43.13051220841425	14.38451323697603
6	15.88744156771237	30.40735651946975	44.374304377528226	9.330897535289647
7	49.800044519838195	5.656015167448322	40.7714213342135	3.7725189784999884
8	47.74798701248858	11.00714620161346	36.39573530653444	4.84913147936352
9	42.8492696443786	8.076205681652453	37.0715157469738	12.003008926995141
10	25.15171286239532	18.78476193400318	43.59029467527383	12.473230528327665
11	20.42677637992309	18.198328203317494	47.013716715664074	14.361178701095342
12	18.07965980702953	16.64550695046784	50.36421833142717	14.910614911075463
13	16.1373667283292	18.711380959325755	53.53648707773318	11.614765234611871
14	13.736672832920119	22.83192225915151	48.21959026397194	15.211814643956432
15	12.498867814459738	18.869810176620945	55.72655608348237	12.904765925436948
16	12.995801318708311	20.55849369430223	48.36021154598976	18.0854934409997
17	13.09773639650289	19.923088141603788	50.59526093998266	16.38391452191067
18	12.954965880917108	20.387783142332992	51.24647871107393	15.410772265675973
19	14.313588529233416	20.765587700243323	48.961996945018	15.958826825505263
20	14.251721305812909	21.313949293439467	52.141173942078154	12.293155458669471
21	13.77228870347485	24.179184672894326	48.927455691247246	13.121070932383578
22	12.411516821590586	19.808871729135163	48.71007606751664	19.069535381757614
23	12.985822734285648	22.825321041764212	49.54351814183406	14.645338082116075
24	14.34076098219974	19.500303195449767	48.05824422969166	18.100691592658833
25	12.137950091726218	25.89504064354194	46.72019281695438	15.246816447777462
26	13.374373460035768	20.321003385042868	49.33488896905871	16.96973418586265
27	16.325731698892376	21.56341390400602	46.558693265990684	15.552161131110923
28	11.808503289094942	21.297369491629503	50.41441828690733	16.479708932368226
29	18.725197460834057	19.149978123872614	45.827800336201534	16.297024079091795
30	16.15087619647065	22.372907375709055	47.685505722334376	13.790710705485917
31	17.08441114838155	22.64969795592536	44.76577192026344	15.500118975429656
32	19.530853015451456	23.67825973487669	42.90330751694441	13.887579732727454
33	14.60219989407349	22.116995064438626	43.77221194513314	19.508593096354744
34	18.525625772380813	22.949055488605225	43.09167248750758	15.433646251506383
35	18.9451868681829	24.61133413673731	38.98279845562217	17.460680539457627
36	18.22550065628382	26.824123611633492	38.05617175446542	16.894203977617266
37	19.966840396380075	26.083098580738262	39.44258092248175	14.507480100399912
38	15.988916095456673	28.14498115582711	38.062465938485865	17.80363681023035
39	20.403288327358975	22.840672710106773	40.78170695200301	15.974332010531246
40	16.889137927064223	26.077725496818367	41.661971614765235	15.371164961352173
41	15.66944787724806	27.1219459774791	37.663015528212526	19.54559061706031
42	16.690180305344686	26.369407195326954	39.82007844702523	17.120334052303136
43	19.619125108421155	23.62038394522525	36.50381105166604	20.256679894687554
44	17.731944519070613	23.801840665034273	39.22151689834893	19.244697917546187
45	16.8895984771145	23.243346970732045	39.32114922589212	20.545905326261334
46	19.54620468379401	25.232309121193747	38.468517566146495	16.75296862886574
47	14.876841240721836	22.29169705017693	45.79003523207884	17.04142647702239
48	17.041272960338965	24.228156494907083	39.61897159173773	19.11159895301622
49	16.19601010139777	21.573546005112103	43.8974815588084	18.33296233468172
50	16.597916778605914	21.22183928338412	41.633878061698354	20.546365876311608
51	16.840626655101744	20.29736181579533	39.031463244268075	23.830548284834855
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	711.0
1	1661.5
2	2612.0
3	35798.5
4	68985.0
5	44375.0
6	19765.0
7	18315.0
8	16865.0
9	15901.0
10	14937.0
11	14324.0
12	13711.0
13	12852.5
14	11994.0
15	10924.5
16	9855.0
17	8859.0
18	7863.0
19	7324.0
20	6785.0
21	6693.5
22	6602.0
23	6506.0
24	6410.0
25	6929.5
26	7900.5
27	8352.0
28	9713.5
29	11075.0
30	12217.0
31	13359.0
32	13876.0
33	14393.0
34	14890.5
35	15388.0
36	16418.5
37	17449.0
38	18525.0
39	19601.0
40	21760.5
41	23920.0
42	26577.0
43	29234.0
44	31159.0
45	33084.0
46	61091.0
47	89098.0
48	69401.0
49	49704.0
50	47468.0
51	45232.0
52	37696.5
53	30161.0
54	25123.5
55	20086.0
56	16481.0
57	12876.0
58	10387.0
59	7898.0
60	6903.5
61	5909.0
62	4841.0
63	3773.0
64	3071.5
65	2370.0
66	1851.0
67	1332.0
68	1124.5
69	917.0
70	771.5
71	626.0
72	523.5
73	421.0
74	339.0
75	223.5
76	190.0
77	129.5
78	69.0
79	49.5
80	30.0
81	25.5
82	21.0
83	15.5
84	10.0
85	7.0
86	4.0
87	4.5
88	5.0
89	5.5
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	651395.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.6018011502334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.35608748981325	31.03042195681875
2	9.976484332008738	7.901734973892581
3	3.598341616374343	4.2750242748679845
4	1.7988780237136544	2.849552391545319
5	1.125596666465867	2.228782768037342
6	0.7000170027927034	1.6633160487827408
7	0.5381286685125701	1.4917605166572265
8	0.4319044844953162	1.3683356408702059
9	0.3535245521793594	1.260018811541907
>10	2.927716215296656	21.406321466192747
>50	0.12534427345529175	3.3933189039026557
>100	0.05618881219419792	3.782647277685288
>500	0.006286860105644523	1.6513000498085597
>1k	0.003929287566027826	3.419149641391207
>5k	0.0	0.0
>10k+	0.0015717150264111307	12.278315278005486
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42612	6.5416529141304425	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG	14222	2.1833142716784746	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTC	11617	1.7834033113548613	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGC	10455	1.6050169252143476	No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	3936	0.6042416659630486	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCT	3517	0.5399181756077341	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTA	2908	0.4464265154015613	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTC	2362	0.36260640625119933	No Hit
GAACTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT	2310	0.3546235387130696	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCC	2288	0.35124617167770705	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1211	0.1859087036283668	No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	1206	0.18514112021123896	TruSeq Adapter, Index 27 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAGTTACT	1181	0.18130320312559967	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	1054	0.16180658433055212	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGC	959	0.14722249940512286	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	842	0.129261047444331	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	784	0.12035707980564787	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	766	0.11759377950398758	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGAGTTACTCGT	758	0.11636564603658302	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	716	0.10991794533270903	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	683	0.10485189477966517	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5351668342557126E-4	0.0	0.0	0.19696190483500794	0.0
2	1.5351668342557126E-4	0.0	0.0	1.1810038455929197	0.0
3	1.5351668342557126E-4	0.0	0.0	1.4564127756583947	0.0
4	1.5351668342557126E-4	0.0	0.0	2.1314256326806316	0.0
5	1.5351668342557126E-4	0.0	0.0	4.825796943482833	0.0
6	1.5351668342557126E-4	0.0	0.0	5.433569493164669	0.0
7	1.5351668342557126E-4	0.0	0.0	6.510028477344775	0.0
8	1.5351668342557126E-4	0.0	0.0	7.653267218815005	0.0
9	1.5351668342557126E-4	0.0	0.0	8.036905410695507	0.0
10	1.5351668342557126E-4	0.0	0.0	11.166343002325778	0.0
11	1.5351668342557126E-4	0.0	0.0	12.675719033765995	0.0
12	1.5351668342557126E-4	0.0	0.0	15.521457794425809	0.0
13	1.5351668342557126E-4	0.0	0.0	16.096377773854574	0.0
14	1.5351668342557126E-4	0.0	0.0	16.378848471357625	0.0
15	1.5351668342557126E-4	0.0	0.0	17.192640410196578	0.0
16	1.5351668342557126E-4	0.0	0.0	18.028231718081962	0.0
17	1.5351668342557126E-4	0.0	0.0	18.88930679541599	0.0
18	1.5351668342557126E-4	0.0	0.0	19.82714021446281	0.0
19	1.5351668342557126E-4	0.0	0.0	20.54145334244199	0.0
20	1.5351668342557126E-4	0.0	0.0	21.093499336040345	0.0
21	3.070333668511425E-4	0.0	0.0	21.716777070748165	0.0
22	3.070333668511425E-4	0.0	0.0	22.373521442442758	0.0
23	3.070333668511425E-4	0.0	0.0	22.988816309612446	0.0
24	3.070333668511425E-4	0.0	0.0	23.516453150546134	0.0
25	3.070333668511425E-4	0.0	0.0	23.972397700320084	0.0
26	3.070333668511425E-4	0.0	0.0	24.40301199732881	0.0
27	3.070333668511425E-4	0.0	0.0	24.8436048787602	0.0
28	3.070333668511425E-4	0.0	0.0	25.268692575165606	0.0
29	3.070333668511425E-4	0.0	0.0	25.72125975790419	0.0
30	3.070333668511425E-4	0.0	0.0	26.236615264163834	0.0
31	3.070333668511425E-4	0.0	0.0	26.68734024670131	0.0
32	3.070333668511425E-4	0.0	0.0	27.179054183713415	0.0
33	3.070333668511425E-4	0.0	0.0	27.62716938263266	0.0
34	3.070333668511425E-4	0.0	0.0	28.10199648446795	0.0
35	3.070333668511425E-4	0.0	0.0	28.580815020072308	0.0
36	3.070333668511425E-4	0.0	0.0	29.051190138088256	0.0
37	3.070333668511425E-4	0.0	0.0	29.506060071078224	0.0
38	3.070333668511425E-4	0.0	0.0	29.959087803867085	0.0
39	3.070333668511425E-4	0.0	0.0	30.431305122084144	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTCGT	20	7.031032E-4	45.000004	14
CTAAGCG	20	7.031032E-4	45.000004	1
CGACAAT	30	2.164048E-6	45.000004	20
GCCCTCG	20	7.031032E-4	45.000004	13
TCATCGA	30	2.164048E-6	45.000004	16
TTCATCG	30	2.164048E-6	45.000004	15
TAGTGCG	20	7.031032E-4	45.000004	1
TATACGG	20	7.031032E-4	45.000004	2
TCGACAA	30	2.164048E-6	45.000004	19
GTTATCA	20	7.031032E-4	45.000004	22
AACACGT	20	7.031032E-4	45.000004	41
CTCACGA	25	3.8889382E-5	45.0	24
CGACGGT	25	3.8889382E-5	45.0	28
CCGATGA	35	1.2108467E-7	45.0	18
TCGATGC	45	3.8380676E-10	45.0	24
ATCCGGC	25	3.8889382E-5	45.0	6
CCGTAAT	25	3.8889382E-5	45.0	23
GTTCCGT	25	3.8889382E-5	45.0	20
GCGATCG	25	3.8889382E-5	45.0	9
TCCGTAA	25	3.8889382E-5	45.0	22
>>END_MODULE