SRR3552161_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552161_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	812090
Sequences flagged as poor quality	0
Sequence length	51
%GC	37

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38262	4.711546749744486	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG	6088	0.7496706030119814	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	5731	0.7057099582558584	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC	4431	0.5456291790318807	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCC	3582	0.4410841162925292	No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	3211	0.39539952468322476	Illumina PCR Primer Index 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	1821	0.22423622997451023	TruSeq Adapter, Index 10 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGATTACCTCGTA	1559	0.19197379600783165	No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1503	0.18507800859510645	RNA PCR Primer, Index 10 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT	1469	0.18089128052309472	TruSeq Adapter, Index 10 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC	1397	0.1720252681353052	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCT	1376	0.16943934785553325	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTC	1296	0.15958822298021155	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGATTACCT	1275	0.1570023027004396	No Hit
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	824	0.10146658621581352	Illumina PCR Primer Index 2 (95% over 22bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG	816	0.10048147372828135	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGT	30	2.164712E-6	45.000004	45
CGAACGG	30	2.164712E-6	45.000004	2
TCACGAC	35	1.2113378E-7	45.0	25
CTCGTAG	20	7.032062E-4	45.0	1
ACGTGCC	20	7.032062E-4	45.0	15
CGGTCGA	20	7.032062E-4	45.0	36
CGTTTTT	19350	0.0	44.23256	1
CGTAAGG	70	0.0	41.785713	2
GACGAAG	65	0.0	41.53846	1
AATGCGG	45	1.9270374E-8	40.0	2
TCCGCAG	40	3.4565528E-7	39.375	1
CACGACG	40	3.4565528E-7	39.375	26
ACGGGTA	80	0.0	39.375	5
TGTACGG	80	0.0	39.375	2
ACGGTCT	40	3.4565528E-7	39.375	30
CGGGAAC	100	0.0	38.25	6
GTTTTTT	23140	0.0	37.940796	2
GCGAACG	30	1.1396267E-4	37.500004	1
TACGGGT	60	1.546141E-10	37.500004	4
TATGACG	30	1.1396267E-4	37.500004	1