##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552161_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	812090
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.987209545740004	30.0	28.0	33.0	25.0	33.0
2	29.019343915083304	31.0	28.0	33.0	16.0	34.0
3	28.665788274698617	31.0	28.0	33.0	16.0	34.0
4	33.4607383418094	35.0	32.0	35.0	28.0	37.0
5	34.47729069438117	35.0	35.0	37.0	32.0	37.0
6	33.66828184068268	35.0	33.0	37.0	28.0	37.0
7	34.866000073883434	35.0	35.0	37.0	32.0	37.0
8	34.47743107291064	35.0	35.0	37.0	32.0	37.0
9	36.6380585895652	39.0	37.0	39.0	32.0	39.0
10	36.21753007671563	37.0	35.0	39.0	32.0	39.0
11	36.733980223866816	39.0	35.0	39.0	32.0	39.0
12	36.764446058934354	39.0	35.0	39.0	32.0	39.0
13	36.89501286803187	39.0	37.0	39.0	33.0	39.0
14	37.68674284869903	39.0	37.0	41.0	33.0	41.0
15	37.95312465367139	39.0	37.0	41.0	33.0	41.0
16	37.459648561120076	39.0	36.0	41.0	32.0	41.0
17	37.45367385388319	39.0	36.0	41.0	32.0	41.0
18	37.073770148628846	39.0	36.0	39.0	32.0	41.0
19	36.27353864719428	37.0	35.0	40.0	31.0	41.0
20	36.29196394488296	37.0	35.0	39.0	32.0	41.0
21	36.42668177172481	37.0	35.0	40.0	32.0	41.0
22	36.850691425827186	38.0	35.0	40.0	33.0	41.0
23	37.09819108719477	38.0	35.0	40.0	33.0	41.0
24	37.04326983462424	38.0	35.0	40.0	33.0	41.0
25	36.29307465921265	37.0	35.0	40.0	32.0	41.0
26	36.58673422896477	38.0	35.0	40.0	33.0	41.0
27	36.83375857355712	38.0	35.0	40.0	33.0	41.0
28	36.70432710660148	38.0	35.0	40.0	33.0	41.0
29	36.68286766245121	38.0	35.0	40.0	33.0	41.0
30	36.06818332943393	38.0	35.0	40.0	31.0	41.0
31	35.854292011969115	38.0	35.0	40.0	30.0	41.0
32	35.3016476006354	38.0	35.0	40.0	25.0	41.0
33	34.69411272149639	38.0	35.0	40.0	20.0	41.0
34	34.07811818887069	38.0	34.0	40.0	15.0	41.0
35	33.745180952850056	38.0	34.0	40.0	12.0	41.0
36	33.4255685946139	38.0	33.0	40.0	10.0	41.0
37	33.370889925993424	38.0	33.0	40.0	10.0	41.0
38	33.197234296691256	38.0	33.0	40.0	10.0	41.0
39	33.10952480636383	38.0	33.0	40.0	10.0	41.0
40	32.987189843490256	38.0	33.0	40.0	10.0	41.0
41	32.83169106872391	38.0	33.0	40.0	10.0	41.0
42	32.90858279254763	38.0	33.0	40.0	10.0	41.0
43	32.87816005615141	38.0	33.0	40.0	10.0	41.0
44	32.84325259515571	38.0	33.0	40.0	9.0	41.0
45	32.88105136130232	38.0	33.0	40.0	10.0	41.0
46	32.73688999987686	38.0	32.0	40.0	8.0	41.0
47	32.63074536073588	38.0	32.0	40.0	8.0	41.0
48	32.630629610018595	38.0	32.0	40.0	8.0	41.0
49	32.63506261621249	38.0	32.0	40.0	8.0	41.0
50	32.59304756861924	38.0	32.0	40.0	8.0	41.0
51	31.71918506569469	36.0	30.0	40.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	11.0
9	25.0
10	23.0
11	28.0
12	25.0
13	31.0
14	54.0
15	67.0
16	96.0
17	215.0
18	459.0
19	870.0
20	1521.0
21	2451.0
22	3820.0
23	6045.0
24	10416.0
25	18746.0
26	28047.0
27	31589.0
28	28642.0
29	23819.0
30	21315.0
31	22216.0
32	25642.0
33	34565.0
34	45702.0
35	64062.0
36	86785.0
37	137409.0
38	170371.0
39	47021.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.56428474676452	14.663891933160118	18.842985383393465	30.928837936681898
2	49.71505621298132	19.630582817175434	17.576500141609923	13.077860828233323
3	25.145981356746173	19.417552241746606	42.06048590673447	13.375980494772746
4	21.88846063859917	18.778091098277287	44.70465096233176	14.628797300791785
5	20.315851691315	22.74809442303193	41.589848415815986	15.346205469837088
6	19.065374527453855	27.247226292652293	42.63480648696573	11.052592692928123
7	56.91438141092736	5.466881749559778	33.551330517553474	4.067406321959389
8	56.20485414178231	6.587939760371388	31.85041066876824	5.356795429078058
9	50.43246438202662	7.247965127017942	33.746382790084844	8.573187700870594
10	26.82867662451206	19.264244110874408	40.8226920661503	13.084387198463224
11	21.33704392370304	20.01637749510522	43.34925931854844	15.297319262643303
12	18.3777660111564	18.002684431528525	47.667623046706645	15.95192651060843
13	18.6870913322415	18.985087859719982	49.22040660517923	13.107414202859289
14	16.51183982070953	21.568422219212156	46.612813850681576	15.306924109396741
15	14.887019911586155	19.82514253346304	50.7399426171976	14.547894937753206
16	16.043788250070808	20.490709157851963	46.75614771761751	16.709354874459727
17	16.3800810255021	20.11562757822409	46.688667512221556	16.81562388405226
18	16.660838084448766	19.39686487950843	48.43490253543327	15.50739450060954
19	16.15215062369934	21.236439310913816	46.723639005528945	15.887771059857897
20	16.77523427206344	21.65375758844463	47.36002167247473	14.210986467017204
21	16.11877993818419	22.750064648006997	46.93457621692177	14.196579196887043
22	15.087859719981775	20.56730165375759	46.06361363888239	18.28122498737825
23	14.714009530963319	21.692423253580266	46.76439803470059	16.829169180755827
24	15.670800034478937	20.294794911894	46.162124887635606	17.872280165991455
25	13.837875112364392	22.917533770887463	45.69431959511876	17.550271521629377
26	13.666711817655678	21.122043123299143	47.766134295459864	17.445110763585316
27	15.918925242276103	21.53702175867207	45.90685761430383	16.637195384747997
28	13.232523488775874	21.54367126796291	47.92498368407443	17.298821559186788
29	15.583740718393283	19.686857368025713	47.523304067283185	17.206097846297823
30	16.52686278614439	21.932175005233407	45.929761479638955	15.61120072898324
31	15.818320629486879	24.097082835646294	43.33596029996675	16.748636234900072
32	15.984558361757934	22.983905724734942	43.995000554125774	17.03653535938135
33	15.938381213904862	22.84340405620067	42.28250563361204	18.935709096282434
34	15.294117647058824	24.97838909480476	42.18288613331035	17.54460712482607
35	15.956728933985149	23.873092883793667	40.33296802078588	19.83721016143531
36	17.09170165868315	26.004630028691402	39.05170609168934	17.851962220936105
37	17.118053417724635	26.841975643093747	39.1583445184647	16.881626420716916
38	15.570318560750657	26.907239345392753	38.68832272285091	18.834119371005677
39	16.67068920932409	24.67546700488862	40.03041534805256	18.623428437734734
40	17.326897265081456	23.854991441835264	39.71591818640791	19.10219310667537
41	16.240564469455357	25.737787683631126	36.31013803888731	21.711509808026204
42	17.15339432821485	23.748353015059905	38.132965558004656	20.965287098720587
43	17.741260205149675	23.286704675590144	38.39106502973808	20.580970089522097
44	17.02015786427613	24.118016476006353	38.323092268098364	20.538733391619154
45	16.838774027509267	23.340885862404413	39.13925796401877	20.681082146067553
46	17.941730596362472	24.2074154342499	38.69866640397001	19.152187565417623
47	15.031092612887734	24.20409067960448	40.990038049969826	19.774778657537958
48	15.94700094817077	23.70808654213203	39.289364479306485	21.05554803039072
49	16.837173219717027	21.807804553682473	40.786981738477266	20.568040488123238
50	16.60123877895307	21.435432033395312	40.11156398921302	21.851765198438596
51	16.23514635077393	21.087071629991748	37.8237633759805	24.854018643253827
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	853.0
1	1721.0
2	2589.0
3	33563.0
4	64537.0
5	41660.0
6	18783.0
7	17770.5
8	16758.0
9	16521.0
10	16284.0
11	15842.0
12	15400.0
13	14663.0
14	13926.0
15	13095.5
16	12265.0
17	11521.0
18	10777.0
19	10125.0
20	9473.0
21	9008.5
22	8544.0
23	8245.0
24	7946.0
25	8204.0
26	9260.5
27	10059.0
28	10840.5
29	11622.0
30	12045.0
31	12468.0
32	13829.5
33	15191.0
34	17551.5
35	19912.0
36	21804.0
37	23696.0
38	25613.0
39	27530.0
40	32318.0
41	37106.0
42	40353.0
43	43600.0
44	62071.0
45	80542.0
46	77994.0
47	75446.0
48	74700.5
49	73955.0
50	63524.5
51	53094.0
52	46076.0
53	39058.0
54	33104.0
55	27150.0
56	22815.5
57	18481.0
58	15396.5
59	12312.0
60	10385.5
61	8459.0
62	7055.5
63	5652.0
64	4822.5
65	3993.0
66	3129.5
67	2266.0
68	1850.0
69	1434.0
70	1160.0
71	886.0
72	756.0
73	626.0
74	526.5
75	346.5
76	266.0
77	197.5
78	129.0
79	101.5
80	74.0
81	55.0
82	36.0
83	21.5
84	7.0
85	6.0
86	5.0
87	5.5
88	6.0
89	5.0
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	812090.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.2328156980514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.25263569018472	34.613344123706995
2	9.918099503128916	8.77410934793673
3	3.701171462426965	4.911397054932579
4	1.8062170898048697	3.195762705760382
5	1.150263252700242	2.543969123046547
6	0.7852637610552249	2.0840656330268663
7	0.55057536331469	1.7047449001370458
8	0.4558929102626697	1.6132341662157563
9	0.36012346303739146	1.4336347292169265
>10	2.885366557744167	23.431428378897692
>50	0.08781709464642824	2.6724148824482676
>100	0.040962544260760576	3.4634376044377433
>500	0.0016833922298826066	0.4988898942879251
>1k	0.0030862190881181116	2.844417009223692
>5k	5.611307432942022E-4	1.4667611095992503
>10k+	2.805653716471011E-4	4.748389337125519
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38262	4.711546749744486	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG	6088	0.7496706030119814	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	5731	0.7057099582558584	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC	4431	0.5456291790318807	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCC	3582	0.4410841162925292	No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	3211	0.39539952468322476	Illumina PCR Primer Index 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	1821	0.22423622997451023	TruSeq Adapter, Index 10 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGATTACCTCGTA	1559	0.19197379600783165	No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1503	0.18507800859510645	RNA PCR Primer, Index 10 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT	1469	0.18089128052309472	TruSeq Adapter, Index 10 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC	1397	0.1720252681353052	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCT	1376	0.16943934785553325	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTC	1296	0.15958822298021155	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGATTACCT	1275	0.1570023027004396	No Hit
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	824	0.10146658621581352	Illumina PCR Primer Index 2 (95% over 22bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG	816	0.10048147372828135	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.23248654705759214	0.0
2	0.0	0.0	0.0	1.016759226194141	0.0
3	0.0	0.0	0.0	1.3162334224039207	0.0
4	0.0	0.0	0.0	1.8924010885492988	0.0
5	0.0	0.0	0.0	3.6265684837887426	0.0
6	0.0	0.0	0.0	4.406531295792338	0.0
7	0.0	0.0	0.0	5.321577657648783	0.0
8	0.0	0.0	0.0	6.627467398933616	0.0
9	0.0	0.0	0.0	7.178637835707865	0.0
10	0.0	0.0	0.0	9.092957677104755	0.0
11	0.0	0.0	0.0	10.991146301518304	0.0
12	0.0	0.0	0.0	12.951520151707323	0.0
13	0.0	0.0	0.0	13.540001723946853	0.0
14	0.0	0.0	0.0	13.788865766109668	0.0
15	0.0	0.0	0.0	14.39606447561231	0.0
16	0.0	0.0	0.0	15.269243556748636	0.0
17	0.0	0.0	0.0	16.28624906106466	0.0
18	0.0	0.0	0.0	17.40644509844968	0.0
19	0.0	0.0	0.0	18.20303168368038	0.0
20	1.2313906094152125E-4	0.0	0.0	18.883251856321344	0.0
21	1.2313906094152125E-4	0.0	0.0	19.636370353039688	0.0
22	1.2313906094152125E-4	0.0	0.0	20.41768769471364	0.0
23	2.462781218830425E-4	0.0	0.0	21.178933369454125	0.0
24	2.462781218830425E-4	0.0	0.0	21.80103190533069	0.0
25	2.462781218830425E-4	0.0	0.0	22.37325912152594	0.0
26	2.462781218830425E-4	0.0	0.0	22.933911265992684	0.0
27	2.462781218830425E-4	0.0	0.0	23.487544483985765	0.0
28	2.462781218830425E-4	0.0	0.0	24.034035636444237	0.0
29	2.462781218830425E-4	0.0	0.0	24.57818714674482	0.0
30	2.462781218830425E-4	0.0	0.0	25.2015170732308	0.0
31	2.462781218830425E-4	0.0	0.0	25.779039269046535	0.0
32	2.462781218830425E-4	0.0	0.0	26.35015823369331	0.0
33	2.462781218830425E-4	0.0	0.0	26.92410939674174	0.0
34	2.462781218830425E-4	0.0	0.0	27.47934342252706	0.0
35	2.462781218830425E-4	0.0	0.0	28.041350096664164	0.0
36	2.462781218830425E-4	0.0	0.0	28.582299991380264	0.0
37	2.462781218830425E-4	0.0	0.0	29.12522011107143	0.0
38	2.462781218830425E-4	0.0	0.0	29.701387777216812	0.0
39	2.462781218830425E-4	0.0	0.0	30.287037151054687	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGT	30	2.164712E-6	45.000004	45
CGAACGG	30	2.164712E-6	45.000004	2
TCACGAC	35	1.2113378E-7	45.0	25
CTCGTAG	20	7.032062E-4	45.0	1
ACGTGCC	20	7.032062E-4	45.0	15
CGGTCGA	20	7.032062E-4	45.0	36
CGTTTTT	19350	0.0	44.23256	1
CGTAAGG	70	0.0	41.785713	2
GACGAAG	65	0.0	41.53846	1
AATGCGG	45	1.9270374E-8	40.0	2
TCCGCAG	40	3.4565528E-7	39.375	1
CACGACG	40	3.4565528E-7	39.375	26
ACGGGTA	80	0.0	39.375	5
TGTACGG	80	0.0	39.375	2
ACGGTCT	40	3.4565528E-7	39.375	30
CGGGAAC	100	0.0	38.25	6
GTTTTTT	23140	0.0	37.940796	2
GCGAACG	30	1.1396267E-4	37.500004	1
TACGGGT	60	1.546141E-10	37.500004	4
TATGACG	30	1.1396267E-4	37.500004	1
>>END_MODULE