##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552160_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	835649
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.68699298389635	31.0	30.0	33.0	28.0	34.0
2	30.778101810688458	31.0	30.0	33.0	26.0	34.0
3	30.30164219666391	31.0	30.0	34.0	25.0	34.0
4	34.53436670180901	35.0	35.0	37.0	32.0	37.0
5	35.23933134605558	35.0	35.0	37.0	33.0	37.0
6	35.19138657498543	36.0	35.0	37.0	33.0	37.0
7	35.722600039011596	37.0	35.0	37.0	35.0	37.0
8	35.35837055988818	37.0	35.0	37.0	33.0	37.0
9	37.34795949016872	39.0	37.0	39.0	34.0	39.0
10	36.811764269448055	39.0	37.0	39.0	32.0	39.0
11	37.11485444247525	39.0	37.0	39.0	34.0	39.0
12	37.34016435130061	39.0	37.0	39.0	34.0	39.0
13	37.35237880976343	39.0	37.0	39.0	34.0	39.0
14	38.454015980393685	40.0	38.0	41.0	34.0	41.0
15	38.614753323464754	40.0	38.0	41.0	34.0	41.0
16	38.36117436866436	40.0	38.0	41.0	34.0	41.0
17	38.14160371160619	40.0	37.0	41.0	33.0	41.0
18	37.75716718382957	39.0	37.0	40.0	34.0	41.0
19	37.00117992123487	37.0	37.0	40.0	33.0	41.0
20	36.629982205447504	37.0	35.0	40.0	33.0	41.0
21	36.73831836093862	38.0	35.0	40.0	33.0	41.0
22	37.026770809275185	38.0	35.0	40.0	33.0	41.0
23	37.09955136666232	38.0	35.0	40.0	33.0	41.0
24	37.036180262287154	38.0	35.0	40.0	33.0	41.0
25	36.571378652999044	38.0	35.0	40.0	33.0	41.0
26	36.72405758877232	38.0	35.0	40.0	33.0	41.0
27	36.92878110307079	38.0	35.0	40.0	33.0	41.0
28	36.80761180830708	38.0	35.0	40.0	33.0	41.0
29	36.76734490198636	38.0	35.0	40.0	33.0	41.0
30	36.31420728080809	38.0	35.0	40.0	32.0	41.0
31	36.009827092475426	38.0	35.0	40.0	31.0	41.0
32	35.44501938014645	38.0	35.0	40.0	25.0	41.0
33	34.64220743398245	38.0	35.0	41.0	18.0	41.0
34	33.92457718491855	38.0	34.0	41.0	15.0	41.0
35	33.54703948667443	38.0	34.0	41.0	10.0	41.0
36	33.21188920228469	38.0	33.0	41.0	10.0	41.0
37	32.849063422561386	38.0	33.0	41.0	9.0	41.0
38	32.8188557636041	38.0	33.0	40.0	10.0	41.0
39	32.7809869933429	38.0	33.0	40.0	9.0	41.0
40	32.71472352626522	38.0	32.0	40.0	8.0	41.0
41	32.533131733538845	38.0	31.0	40.0	8.0	41.0
42	32.37837776386976	38.0	31.0	40.0	8.0	41.0
43	32.40195105839892	38.0	31.0	40.0	8.0	41.0
44	32.375698409260345	38.0	31.0	40.0	8.0	41.0
45	32.35920703548978	38.0	31.0	40.0	8.0	41.0
46	32.226398882784515	38.0	31.0	40.0	8.0	41.0
47	32.203815238216045	38.0	31.0	40.0	8.0	41.0
48	32.17103113867186	38.0	31.0	40.0	8.0	41.0
49	32.11076899511637	38.0	30.0	40.0	8.0	41.0
50	32.012460973446984	37.0	30.0	40.0	8.0	41.0
51	30.798439296881824	35.0	27.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	22.0
10	26.0
11	24.0
12	17.0
13	30.0
14	40.0
15	60.0
16	112.0
17	225.0
18	419.0
19	873.0
20	1419.0
21	2329.0
22	3521.0
23	6036.0
24	10236.0
25	19198.0
26	30226.0
27	35592.0
28	32473.0
29	26713.0
30	22481.0
31	21412.0
32	22530.0
33	28560.0
34	43118.0
35	55391.0
36	67538.0
37	99692.0
38	184669.0
39	120656.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.2101181237577	14.071936901737454	18.42149036258046	32.29645461192439
2	50.35894257038541	20.44040021588011	17.209378578805214	11.991278634929259
3	24.191377001587988	20.02300008735725	43.435102537069994	12.35052037398477
4	21.22577780862539	18.034844773343835	46.66815852110156	14.071218896929214
5	19.746328901249207	22.205734704403405	43.014591054378094	15.033345339969292
6	18.335210118123758	27.354666851752352	44.107873042389805	10.202249987734085
7	55.21516809090898	5.4233296515642335	35.387704646328785	3.973797611198003
8	54.365768402762406	7.066842657622997	33.70912907213435	4.858259867480246
9	48.767245577987886	7.252087898148625	35.51515049979118	8.465516024072308
10	25.979567976506885	18.767209677747477	42.31178401458028	12.941438331165358
11	20.67052075692067	19.325458416153193	45.04881834358684	14.955202483339297
12	18.042623158766418	17.375118022040354	49.16083188037083	15.4214269388224
13	17.990567810169104	18.387026131784996	50.894574157331604	12.727831900714296
14	16.119566947366657	21.060397367794373	47.815410537199234	15.00462514763974
15	14.542110383665868	19.242409193333565	52.31981370168576	13.89566672131481
16	15.56155754389702	20.09300555616054	47.9596098361872	16.385827063755237
17	15.8783173317984	19.32713376070575	48.27457461206798	16.519974295427865
18	16.11083122219975	19.03143544717938	49.77556366369134	15.082169666929538
19	15.594585765076008	20.917993080827	48.07580694765386	15.411614206443137
20	16.210035553204754	21.110897039307172	48.75719351067254	13.921873896815528
21	15.488680055860774	22.439445269485155	48.33763936772497	13.734235306929104
22	14.605414474258929	20.038078188330267	47.211568493470345	18.14493884394046
23	14.30110010303369	21.446803622094922	48.039069034965635	16.21302723990575
24	15.324376622242115	19.6777594420624	47.27690693102008	17.72095700467541
25	13.35189774654191	22.78277123529137	46.82013620551213	17.045194812654596
26	13.313604156769172	20.52584278806054	48.99138274562645	17.16917030954384
27	15.531042339546866	21.08816022037961	46.92891393396031	16.451883506113212
28	12.834694949673848	20.76338271211956	49.37276296626933	17.02915937193726
29	15.321863605413277	19.162231989746893	48.697599111588715	16.818305293251115
30	16.208838878524357	21.42322913089108	47.210491486257986	15.157440504326578
31	15.476473974120713	23.50149404833848	44.56823379193896	16.45379818560185
32	15.773249294859445	22.408092392858727	45.29210230611177	16.526556006170054
33	15.603441157710954	22.370636475362264	43.313520389541544	18.712401977385245
34	14.95903184231657	24.694698372163433	43.105179327684226	17.241090457835767
35	15.938988738094583	23.6638828024685	40.90449459043211	19.49263386900481
36	16.613554255434998	26.187909038364193	39.68484375617035	17.513692950030453
37	17.013243598687964	26.629840997835213	39.642122470080146	16.714792933396676
38	15.220624927451599	26.88712605412081	39.117141287789494	18.7751077306381
39	16.37122763265438	24.794860042912752	40.62267770319835	18.211234621234514
40	16.988472432803725	23.871864861921694	40.245725178872945	18.893937526401636
41	15.935638048989468	25.806169815317197	36.664915532717686	21.59327660297565
42	17.207942569188738	23.64377866783781	38.521795634291436	20.62648312868202
43	17.654421892445274	23.15733041025598	38.621119632764476	20.56712806453427
44	16.88292572599261	24.017978840398303	38.96456526603873	20.134530167570354
45	16.827280353354098	23.033713915770857	39.69812684512277	20.440878885752273
46	18.09467850736374	24.29668437346302	38.99376412824044	18.614872990932795
47	14.869041906350633	23.960777790675273	41.76047598932088	19.40970431365322
48	15.932646362288471	23.459610434524546	39.94703517864558	20.660708024541403
49	16.718502624905913	21.536314888188702	41.59976257974341	20.14541990716198
50	16.489818093481833	21.21572574131005	40.803136245002385	21.491319920205733
51	16.25790254042068	20.938695552797885	38.32805400353498	24.47534790324646
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	957.0
1	1958.0
2	2959.0
3	37756.0
4	72553.0
5	46674.0
6	20795.0
7	19890.5
8	18986.0
9	18513.0
10	18040.0
11	17403.5
12	16767.0
13	16343.0
14	15919.0
15	14686.5
16	13454.0
17	12606.0
18	11758.0
19	10912.5
20	10067.0
21	9463.0
22	8859.0
23	8622.0
24	8385.0
25	8595.5
26	9456.5
27	10107.0
28	10910.5
29	11714.0
30	12129.5
31	12545.0
32	13913.0
33	15281.0
34	17582.5
35	19884.0
36	21874.0
37	23864.0
38	25664.0
39	27464.0
40	32322.5
41	37181.0
42	40522.0
43	43863.0
44	64393.5
45	84924.0
46	79659.5
47	74395.0
48	74267.5
49	74140.0
50	63563.0
51	52986.0
52	45705.0
53	38424.0
54	32583.5
55	26743.0
56	22275.0
57	17807.0
58	15038.5
59	12270.0
60	10294.0
61	8318.0
62	6979.5
63	5641.0
64	4769.5
65	3898.0
66	3027.5
67	2157.0
68	1760.0
69	1363.0
70	1120.0
71	877.0
72	745.0
73	613.0
74	476.5
75	280.0
76	220.0
77	175.0
78	130.0
79	106.0
80	82.0
81	62.5
82	43.0
83	34.5
84	26.0
85	20.5
86	15.0
87	16.0
88	17.0
89	11.0
90	5.0
91	3.5
92	2.0
93	2.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	835649.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.859736558216845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.21855298218507	34.30645127763564
2	9.998840310683896	8.770930038285494
3	3.6430408858610313	4.793484405740332
4	1.8237999640183893	3.199655438269277
5	1.138096854958717	2.495831411811222
6	0.7794132873306171	2.051091687137678
7	0.5785579367678608	1.77627790832127
8	0.49071125086278167	1.7217972951195732
9	0.37667919488193713	1.4868945229034687
>10	2.812179424578146	22.747232103916453
>50	0.09203674773860267	2.7506571797822703
>100	0.04173428264425776	3.4636808272839805
>500	0.0027638597777351593	0.7646693962263724
>1k	0.0024874737999616434	2.133873713026765
>5k	8.291579333205479E-4	2.389046363479605
>10k+	2.7638597777351594E-4	5.148426431060599
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42471	5.082397035118811	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG	7469	0.8937963187893482	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTC	6718	0.8039260502914501	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC	5521	0.6606840910477964	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCC	3508	0.41979347788365684	No Hit
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	3234	0.3870045916407487	Illumina PCR Primer Index 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	1786	0.2137260979191024	TruSeq Adapter, Index 10 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCT	1690	0.20223802098728055	TruSeq Adapter, Index 10 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGATTACCTCGTA	1612	0.19290395848017527	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCT	1600	0.19146794886369756	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTC	1435	0.17172281663712874	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGC	1393	0.1666967829794567	No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	1345	0.16095274451354574	RNA PCR Primer, Index 10 (95% over 23bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCG	864	0.10339269238639667	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22545350978700388	0.0
2	0.0	0.0	0.0	0.9718195079513049	0.0
3	0.0	0.0	0.0	1.2757748767724248	0.0
4	0.0	0.0	0.0	1.8597521208067023	0.0
5	0.0	0.0	0.0	3.6757059483108336	0.0
6	0.0	0.0	0.0	4.452347815889207	0.0
7	0.0	0.0	0.0	5.363136915140208	0.0
8	0.0	0.0	0.0	6.681513410534806	0.0
9	0.0	0.0	0.0	7.21068295420685	0.0
10	0.0	0.0	0.0	9.285357847613053	0.0
11	1.1966746803981097E-4	0.0	0.0	11.165692772922602	0.0
12	2.3933493607962195E-4	0.0	0.0	13.218947189549679	0.0
13	2.3933493607962195E-4	0.0	0.0	13.826857927191918	0.0
14	2.3933493607962195E-4	0.0	0.0	14.07983495462808	0.0
15	2.3933493607962195E-4	0.0	0.0	14.667162887767471	0.0
16	2.3933493607962195E-4	0.0	0.0	15.549112127220878	0.0
17	3.5900240411943293E-4	0.0	0.0	16.54211277701523	0.0
18	3.5900240411943293E-4	0.0	0.0	17.668422986205933	0.0
19	3.5900240411943293E-4	0.0	0.0	18.360818956284277	0.0
20	3.5900240411943293E-4	0.0	0.0	19.022460387076393	0.0
21	3.5900240411943293E-4	0.0	0.0	19.761646337158304	0.0
22	3.5900240411943293E-4	0.0	0.0	20.51818407010599	0.0
23	3.5900240411943293E-4	0.0	0.0	21.275320140393873	0.0
24	3.5900240411943293E-4	0.0	0.0	21.876290164889806	0.0
25	3.5900240411943293E-4	0.0	0.0	22.440402609229473	0.0
26	3.5900240411943293E-4	0.0	0.0	22.989197617660047	0.0
27	3.5900240411943293E-4	0.0	0.0	23.515136139695016	0.0
28	3.5900240411943293E-4	0.0	0.0	24.027432570373445	0.0
29	3.5900240411943293E-4	0.0	0.0	24.56198715010728	0.0
30	3.5900240411943293E-4	0.0	0.0	25.171333897366	0.0
31	3.5900240411943293E-4	0.0	0.0	25.732215320068594	0.0
32	3.5900240411943293E-4	0.0	0.0	26.269881253971462	0.0
33	3.5900240411943293E-4	0.0	0.0	26.812932223936127	0.0
34	3.5900240411943293E-4	0.0	0.0	27.34258043748033	0.0
35	3.5900240411943293E-4	0.0	0.0	27.875818675065727	0.0
36	3.5900240411943293E-4	0.0	0.0	28.39314113940183	0.0
37	3.5900240411943293E-4	0.0	0.0	28.913933960311088	0.0
38	3.5900240411943293E-4	0.0	0.0	29.462489633805582	0.0
39	4.786698721592439E-4	0.0	0.0	30.023849726380334	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGCGT	20	7.03218E-4	45.0	12
CGACAAC	20	7.03218E-4	45.0	29
TCGATAG	20	7.03218E-4	45.0	1
AGTTCGT	20	7.03218E-4	45.0	28
CGTAAGG	95	0.0	45.0	2
ATAACGG	25	3.8898892E-5	45.0	2
TATCGAG	25	3.8898892E-5	45.0	1
TACCGTA	20	7.03218E-4	45.0	28
AGCGACT	30	2.1647884E-6	44.999996	19
CGTTTTT	21280	0.0	44.080124	1
CCTATGC	80	0.0	42.1875	35
CGAATAT	100	0.0	40.5	14
GCTACGA	100	0.0	40.5	10
ACGGGCG	95	0.0	40.263157	5
AACGAGC	45	1.9272193E-8	40.0	15
CACAACG	45	1.9272193E-8	40.0	12
GCGTAAG	90	0.0	40.0	1
TACGGCT	735	0.0	39.183674	7
AGCCGTA	35	6.2462404E-6	38.57143	13
GTTTTTT	25370	0.0	38.224277	2
>>END_MODULE