FastQCFastQC Report
Sat 18 Jun 2016
SRR3552157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831399
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT635297.641216792418562No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC160111.925790144082444No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCG142211.7104903902939501No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGC100461.2083247634408991No Hit
GAACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT51930.6246098443707534No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC35510.42711141100723Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT34160.4108737200790475No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGCTAGTT30680.3690165612419548No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTA30200.3632431600230455No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTC30050.3614389721421363No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCC22360.26894427344752636No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT17120.2059179768077662No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGC11640.14000497955855132No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA11330.13627632460467237No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA9060.10897294800691365No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGT8950.10764987689424693No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA8730.10500373466891348No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAC253.8898703E-545.00000413
CGGCGAA700.045.031
CACGGCT207.0321606E-445.018
TCTACGG207.0321606E-445.02
CACGTTG207.0321606E-445.01
CGTCACG207.0321606E-445.036
TATCGAG207.0321606E-445.01
CGTGCGG302.1647757E-644.9999962
GAGTACG302.1647757E-644.9999961
TTACCGG302.1647757E-644.9999962
CGTTTTT309050.044.0608371
CACGACC1800.041.2527
TACGGCT12350.041.174097
CCGATGA8850.040.932218
ACCCTCG556.002665E-1140.90909239
AGTACGG556.002665E-1140.9090922
CGATGAA8900.040.70224819
ACACGAC1850.040.13513626
GAATCTG19300.039.7538871
GTTTTTT349850.039.4369052
Produced by FastQC (version 0.11.5)