##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552157_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	831399
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.47309174054816	31.0	28.0	33.0	25.0	33.0
2	29.409209056060927	31.0	30.0	33.0	16.0	34.0
3	29.21704139648953	31.0	28.0	34.0	16.0	34.0
4	33.551543843569696	35.0	33.0	35.0	28.0	37.0
5	34.570282138900815	35.0	35.0	37.0	32.0	37.0
6	34.11759215490998	35.0	35.0	37.0	30.0	37.0
7	34.96315246951223	35.0	35.0	37.0	32.0	37.0
8	34.43569934532036	35.0	35.0	37.0	31.0	37.0
9	36.64438374354552	39.0	37.0	39.0	32.0	39.0
10	36.449450865348645	38.0	35.0	39.0	32.0	39.0
11	36.865331808193176	39.0	37.0	39.0	33.0	39.0
12	36.92927703786028	39.0	37.0	39.0	33.0	39.0
13	36.86115571464483	39.0	37.0	39.0	33.0	39.0
14	37.823431348846945	40.0	37.0	41.0	32.0	41.0
15	38.039843685162	40.0	37.0	41.0	33.0	41.0
16	37.7580884749681	40.0	36.0	41.0	32.0	41.0
17	37.46064765533757	39.0	36.0	41.0	32.0	41.0
18	37.0094852170859	39.0	36.0	39.0	32.0	40.0
19	36.00194611732754	37.0	35.0	39.0	31.0	40.0
20	35.342521460814844	35.0	35.0	39.0	31.0	40.0
21	35.6397012745986	35.0	35.0	39.0	31.0	40.0
22	36.03077583687255	35.0	35.0	39.0	32.0	41.0
23	36.18835721476692	35.0	35.0	40.0	33.0	41.0
24	36.01776643945927	35.0	35.0	40.0	32.0	41.0
25	35.476500452851155	35.0	35.0	39.0	31.0	41.0
26	35.62809673814859	35.0	35.0	40.0	31.0	41.0
27	35.80904716026841	35.0	35.0	40.0	32.0	41.0
28	35.57506564236907	35.0	35.0	40.0	31.0	41.0
29	35.48182521268368	35.0	35.0	40.0	31.0	41.0
30	34.819070025342825	35.0	35.0	40.0	27.0	41.0
31	34.47495366244126	35.0	35.0	40.0	24.0	41.0
32	33.448055626720745	35.0	33.0	40.0	18.0	41.0
33	32.35277887031377	35.0	32.0	40.0	12.0	41.0
34	31.32018802043303	35.0	28.0	40.0	8.0	41.0
35	30.743442077750874	35.0	24.0	40.0	7.0	41.0
36	30.226792430589885	35.0	22.0	40.0	7.0	41.0
37	30.23573278293575	35.0	22.0	40.0	7.0	41.0
38	30.03022736375675	35.0	21.0	40.0	7.0	41.0
39	29.82507195702665	35.0	21.0	40.0	7.0	41.0
40	29.6796736584961	35.0	21.0	40.0	7.0	41.0
41	29.323210636529513	35.0	20.0	40.0	7.0	41.0
42	29.441209335108653	35.0	18.0	40.0	7.0	41.0
43	29.361393266049152	35.0	18.0	40.0	7.0	41.0
44	29.377475796819578	35.0	18.0	40.0	7.0	41.0
45	29.409160944384105	35.0	20.0	40.0	7.0	41.0
46	29.23723627283651	35.0	20.0	40.0	7.0	41.0
47	29.059325305900057	35.0	20.0	40.0	7.0	41.0
48	29.081732116589027	35.0	20.0	40.0	7.0	41.0
49	29.11567249900469	35.0	20.0	40.0	7.0	41.0
50	29.021612968021373	35.0	20.0	40.0	7.0	41.0
51	28.28385408209536	34.0	18.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	17.0
10	14.0
11	24.0
12	21.0
13	28.0
14	45.0
15	93.0
16	158.0
17	340.0
18	751.0
19	1602.0
20	3207.0
21	5002.0
22	7147.0
23	10481.0
24	16427.0
25	29359.0
26	43510.0
27	49093.0
28	44662.0
29	36934.0
30	30938.0
31	29563.0
32	32244.0
33	38974.0
34	47357.0
35	71449.0
36	80035.0
37	104619.0
38	115397.0
39	31897.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.168671119402354	10.7741289080213	13.00627015428212	43.05092981829423
2	55.46855360663171	21.763076453062848	12.558109884664281	10.210260055641154
3	18.286526685742945	21.433752025200896	50.59856939928963	9.681151889766525
4	15.615967784421198	13.875648154496217	58.94558449071986	11.562799570362726
5	20.749724259952202	15.595039205002653	51.02363606403183	12.631600471013316
6	14.525516629199696	24.274505983288407	52.240620929301095	8.959356458210799
7	43.610709178144305	3.582515735525301	49.79714914258978	3.0096259437406108
8	42.125742272964004	8.9123272941151	44.503661900002285	4.458268532918611
9	38.58339978758695	4.649753006679103	44.912851711392484	11.853995494341465
10	23.511334509663833	14.984983142871233	50.273575022341866	11.230107325123075
11	19.276664994785897	15.172378124101666	53.27009053414786	12.280866346964574
12	14.883226946387957	13.97331485844943	56.0096896917124	15.13376850345021
13	15.57495257992853	15.303843281023912	58.75037136200548	10.37083277704207
14	12.76210339439908	19.62956414429173	54.818805411120294	12.789527050188898
15	10.948052619740944	15.233239395284334	60.20743349462773	13.611274490346995
16	12.783392811393806	17.168291037155445	53.62262884607751	16.42568730537323
17	12.948776700477147	17.4624939409357	55.65558775028596	13.933141608301188
18	14.11813100569041	15.217242262740271	55.69636239639452	14.968264335174808
19	12.754646084491322	17.252727029981994	53.78837357273704	16.204253312789646
20	14.725300367212373	17.35243848020024	56.96951764435608	10.952743508231306
21	12.971629746968663	20.491123997021887	55.05419178998291	11.483054466026541
22	12.818875173051689	14.66564188795031	56.4341549604943	16.081327978503705
23	13.327776434660132	19.17057874738844	55.35789674993595	12.143748068015478
24	13.547045401786628	17.344740611908364	53.965304264258194	15.142909722046817
25	11.716275819432067	21.978857323619586	52.962777198432995	13.342089658515347
26	12.290969799097667	15.848828300250542	56.26299767019205	15.597204230459743
27	14.97030908143984	16.692225994979545	52.94184861901445	15.39561630456616
28	11.064122040079432	17.867473980603776	55.9892422290621	15.079161750254691
29	13.737447362818575	15.532253466747012	55.48707660220905	15.243222568225365
30	13.022748403594422	18.345222931468523	53.763355500788435	14.868673164148621
31	16.549695152387724	16.68921901517803	50.515817315151935	16.245268517282316
32	16.14772209252116	18.444092427342348	49.71487817522032	15.693307304916171
33	14.522990766166425	18.463096539687925	48.508237320468275	18.50567537367738
34	16.066172800304066	22.00086841576668	46.838521576282865	15.094437207646388
35	17.142310731670353	21.452515579162352	43.10060512461526	18.30456856455204
36	18.090832440260332	23.14640744095194	43.802915327057164	14.959844791730564
37	17.04548598206156	22.58121551745913	41.20488477854797	19.168413721931348
38	19.57002594422173	22.71941630913677	40.40791485195436	17.302642894687146
39	18.492564941742774	23.509530321782922	40.10565324230604	17.892251494168264
40	20.2379362977343	21.846670491544977	41.96577094752339	15.949622263197332
41	17.96850850193469	25.06630390462341	40.05633877356119	16.908848819880706
42	19.997017076036897	21.997741156773102	41.56788738018689	16.43735438700311
43	19.25898395355299	21.857014502062185	40.624176839279336	18.25982470510549
44	18.90764843354394	21.908253437880006	39.73771919379263	19.44637893478342
45	18.54885560362714	20.8828733255633	39.994394989649976	20.573876081159586
46	19.748881102815858	22.49509561594373	39.89336046831906	17.862662812921354
47	15.440480443204768	22.01085158870771	45.56115655659918	16.987511411488345
48	17.53141391798643	22.990525608041384	40.90623154466147	18.571828929310715
49	17.297711447812663	20.298075893764604	44.16700044142463	18.237212216998095
50	16.24045734959989	20.59083544724013	43.339720158431746	19.828987044728223
51	16.56665451846827	19.70401696417725	40.87291420846068	22.856414308893804
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	652.0
1	1511.5
2	2371.0
3	51641.5
4	100912.0
5	64845.0
6	28778.0
7	27401.0
8	26024.0
9	25525.5
10	25027.0
11	24021.0
12	23015.0
13	21792.5
14	20570.0
15	19184.0
16	17798.0
17	16649.0
18	15500.0
19	14319.0
20	13138.0
21	12066.5
22	10995.0
23	10530.0
24	10065.0
25	10109.0
26	10366.0
27	10579.0
28	10798.5
29	11018.0
30	12101.0
31	13184.0
32	14289.5
33	15395.0
34	16258.5
35	17122.0
36	17718.5
37	18315.0
38	19742.5
39	21170.0
40	22245.0
41	23320.0
42	24217.0
43	25114.0
44	27729.5
45	30345.0
46	62119.0
47	93893.0
48	71166.0
49	48439.0
50	47644.0
51	46849.0
52	41794.5
53	36740.0
54	33268.5
55	29797.0
56	26294.5
57	22792.0
58	19906.0
59	17020.0
60	14630.0
61	12240.0
62	10835.5
63	9431.0
64	8311.0
65	7191.0
66	6164.0
67	5137.0
68	4363.5
69	3590.0
70	3056.5
71	2523.0
72	2250.0
73	1977.0
74	1687.5
75	1147.5
76	897.0
77	705.0
78	513.0
79	374.0
80	235.0
81	176.5
82	118.0
83	77.0
84	36.0
85	23.0
86	10.0
87	9.5
88	9.0
89	5.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	831399.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.27505488491719
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.31780614970275	35.118002206293106
2	8.635465853020373	7.6467144919861045
3	3.670951064481255	4.875946795792582
4	2.2030346394024014	3.9015791829166027
5	1.4409269216685117	3.189855927101406
6	0.9946113216465077	2.642188251303548
7	0.7426990312820451	2.3018128261091158
8	0.5689097535521624	2.015080845046936
9	0.44450151622391876	1.771229612451862
>10	1.8498015293262158	12.752708405999346
>50	0.07031554932878094	2.1752434633499096
>100	0.05246191008897124	4.281042264441323
>500	0.004669384667576152	1.360574407135818
>1k	0.0024720271769520805	2.712514493311085
>5k	2.746696863280089E-4	0.6315215316639526
>10k+	0.0010986787453120357	12.623985295097235
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63529	7.641216792418562	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTC	16011	1.925790144082444	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCG	14221	1.7104903902939501	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGC	10046	1.2083247634408991	No Hit
GAACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCT	5193	0.6246098443707534	No Hit
GCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTTCTGC	3551	0.42711141100723	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCT	3416	0.4108737200790475	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGCTAGTT	3068	0.3690165612419548	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTA	3020	0.3632431600230455	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTC	3005	0.3614389721421363	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCC	2236	0.26894427344752636	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGCCGTCTT	1712	0.2059179768077662	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGTATGC	1164	0.14000497955855132	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1133	0.13627632460467237	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	906	0.10897294800691365	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCGCTAGTTCGT	895	0.10764987689424693	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	873	0.10500373466891348	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07890314999176087	0.0
2	0.0	0.0	0.0	0.6425314439877845	0.0
3	0.0	0.0	0.0	0.8140495718662158	0.0
4	0.0	0.0	0.0	1.6259341182753406	0.0
5	0.0	0.0	0.0	4.0991148654256255	0.0
6	0.0	0.0	0.0	4.724566664140804	0.0
7	0.0	0.0	0.0	5.515642910323443	0.0
8	0.0	0.0	0.0	6.368542661225236	0.0
9	0.0	0.0	0.0	6.6643091945022785	0.0
10	0.0	0.0	0.0	9.0421085423485	0.0
11	0.0	0.0	0.0	9.939992711080961	0.0
12	0.0	0.0	0.0	11.941197908585409	0.0
13	0.0	0.0	0.0	12.342088455723426	0.0
14	0.0	0.0	0.0	12.549209224451797	0.0
15	0.0	0.0	0.0	13.118851478050852	0.0
16	0.0	0.0	0.0	13.569176773125779	0.0
17	0.0	0.0	0.0	13.975840721482706	0.0
18	0.0	0.0	0.0	14.428571600398845	0.0
19	0.0	0.0	0.0	15.103458147050935	0.0
20	0.0	0.0	0.0	15.44493077331101	0.0
21	0.0	0.0	0.0	15.760904210854235	0.0
22	0.0	0.0	0.0	16.140625620189585	0.0
23	0.0	0.0	0.0	16.46646195148178	0.0
24	0.0	0.0	0.0	16.729873382094517	0.0
25	0.0	0.0	0.0	16.96489892338095	0.0
26	0.0	0.0	0.0	17.216883830747932	0.0
27	0.0	0.0	0.0	17.49593155632855	0.0
28	0.0	0.0	0.0	17.748277301271713	0.0
29	0.0	0.0	0.0	18.01349291976536	0.0
30	0.0	0.0	0.0	18.353642474912768	0.0
31	0.0	0.0	0.0	18.62559372816181	0.0
32	1.2027919206061109E-4	0.0	0.0	18.918112723253216	0.0
33	1.2027919206061109E-4	0.0	0.0	19.20281357086068	0.0
34	1.2027919206061109E-4	0.0	0.0	19.46826974773845	0.0
35	1.2027919206061109E-4	0.0	0.0	19.761991534750464	0.0
36	1.2027919206061109E-4	0.0	0.0	20.010127507971504	0.0
37	1.2027919206061109E-4	0.0	0.0	20.27113335474303	0.0
38	1.2027919206061109E-4	0.0	0.0	20.569305471861284	0.0
39	2.4055838412122217E-4	0.0	0.0	20.98583231396718	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAAC	25	3.8898703E-5	45.000004	13
CGGCGAA	70	0.0	45.0	31
CACGGCT	20	7.0321606E-4	45.0	18
TCTACGG	20	7.0321606E-4	45.0	2
CACGTTG	20	7.0321606E-4	45.0	1
CGTCACG	20	7.0321606E-4	45.0	36
TATCGAG	20	7.0321606E-4	45.0	1
CGTGCGG	30	2.1647757E-6	44.999996	2
GAGTACG	30	2.1647757E-6	44.999996	1
TTACCGG	30	2.1647757E-6	44.999996	2
CGTTTTT	30905	0.0	44.060837	1
CACGACC	180	0.0	41.25	27
TACGGCT	1235	0.0	41.17409	7
CCGATGA	885	0.0	40.9322	18
ACCCTCG	55	6.002665E-11	40.909092	39
AGTACGG	55	6.002665E-11	40.909092	2
CGATGAA	890	0.0	40.702248	19
ACACGAC	185	0.0	40.135136	26
GAATCTG	1930	0.0	39.753887	1
GTTTTTT	34985	0.0	39.436905	2
>>END_MODULE