##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552155_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	642515
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.21048068916679	31.0	28.0	33.0	25.0	33.0
2	29.001288685867255	31.0	28.0	33.0	16.0	34.0
3	28.779925760488084	31.0	28.0	33.0	16.0	34.0
4	33.47173529022669	35.0	32.0	35.0	28.0	37.0
5	34.49671058263231	35.0	35.0	35.0	32.0	37.0
6	33.87128705166416	35.0	33.0	37.0	28.0	37.0
7	34.808152338855905	35.0	35.0	37.0	32.0	37.0
8	34.02654568375836	35.0	35.0	37.0	28.0	37.0
9	36.30287075009922	39.0	35.0	39.0	31.0	39.0
10	36.20069570360225	37.0	35.0	39.0	32.0	39.0
11	36.72506011532805	39.0	35.0	39.0	32.0	39.0
12	36.80738504159436	39.0	35.0	39.0	33.0	39.0
13	36.742360878734345	39.0	35.0	39.0	32.0	39.0
14	37.658565169684756	39.0	37.0	41.0	32.0	41.0
15	37.881722605697924	40.0	37.0	41.0	32.0	41.0
16	37.537847365431155	39.0	36.0	41.0	32.0	41.0
17	37.239524369080875	39.0	36.0	41.0	32.0	41.0
18	36.93707695540182	39.0	36.0	39.0	32.0	41.0
19	36.06276273705672	37.0	35.0	39.0	31.0	41.0
20	35.56354637634919	35.0	35.0	39.0	30.0	40.0
21	35.93120004980428	36.0	35.0	39.0	31.0	40.0
22	36.32924056247714	37.0	35.0	40.0	32.0	41.0
23	36.46008731313666	37.0	35.0	40.0	33.0	41.0
24	36.33997649860314	37.0	35.0	40.0	32.0	41.0
25	35.772360178361595	36.0	35.0	40.0	31.0	41.0
26	35.957557411111026	36.0	35.0	40.0	31.0	41.0
27	36.10542477607527	37.0	35.0	40.0	32.0	41.0
28	35.92918920180852	37.0	35.0	40.0	31.0	41.0
29	35.91009081500043	37.0	35.0	40.0	31.0	41.0
30	35.318504626351135	36.0	35.0	40.0	29.0	41.0
31	35.060923091289695	36.0	35.0	40.0	27.0	41.0
32	34.30632592235201	37.0	34.0	40.0	21.0	41.0
33	33.55319019789421	37.0	33.0	40.0	15.0	41.0
34	32.76218765320654	37.0	33.0	40.0	11.0	41.0
35	32.26482338933722	37.0	31.0	40.0	9.0	41.0
36	31.926582258779952	37.0	31.0	40.0	8.0	41.0
37	31.837476167871568	37.0	30.0	40.0	7.0	41.0
38	31.67054776931278	36.0	30.0	40.0	8.0	41.0
39	31.527472510369407	36.0	29.0	40.0	7.0	41.0
40	31.361737858260117	36.0	27.0	40.0	8.0	41.0
41	31.136199154883542	35.0	26.0	40.0	7.0	41.0
42	31.232808572562508	36.0	27.0	40.0	7.0	41.0
43	31.230766596888788	36.0	26.0	40.0	7.0	41.0
44	31.278542913395018	37.0	26.0	40.0	7.0	41.0
45	31.274757787755927	36.0	26.0	40.0	7.0	41.0
46	31.111967813980996	36.0	25.0	40.0	7.0	41.0
47	30.95786557512276	35.0	25.0	40.0	7.0	41.0
48	31.05251550547458	35.0	25.0	40.0	7.0	41.0
49	31.10733912826938	36.0	26.0	40.0	7.0	41.0
50	30.989091305261354	35.0	25.0	40.0	7.0	41.0
51	30.366050598040513	35.0	24.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	16.0
10	16.0
11	11.0
12	21.0
13	21.0
14	39.0
15	44.0
16	79.0
17	213.0
18	590.0
19	1398.0
20	2351.0
21	3576.0
22	5121.0
23	6866.0
24	10300.0
25	16792.0
26	25344.0
27	29643.0
28	28181.0
29	23761.0
30	21452.0
31	22109.0
32	24182.0
33	30317.0
34	38435.0
35	56640.0
36	67326.0
37	92377.0
38	105239.0
39	30046.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.75258165179023	11.975128985315518	14.765725313805902	35.50656404908835
2	49.61705174198268	25.60173692442978	13.966366543971736	10.814844789615808
3	20.239060566679377	24.85155988576142	44.30215636989019	10.607223177669004
4	17.66340085445476	15.538781195769749	54.67545504774207	12.122362902033416
5	23.65843598982125	18.353034559504447	44.6355338007673	13.352995649907006
6	15.356684279744442	29.20523256266391	45.810292366715174	9.627790790876478
7	46.5856828245255	4.835684770005369	45.04626351135771	3.5323688941114213
8	44.5030855310771	12.059484992568267	38.30260772122052	5.134821755134122
9	40.39890119296825	6.247791880345206	38.898547115631544	14.454759811054995
10	26.301954039983507	16.74373360933208	45.178089227488854	11.776223123195567
11	21.84338108837926	16.96831980576329	47.78721119351299	13.401087912344458
12	16.54436083204283	15.741578017633827	50.87009641798246	16.84396473234088
13	17.407375703290974	16.721321681205886	54.60183808938312	11.269464526120013
14	13.89788565247504	22.818922515427655	49.132860711423085	14.150331120674226
15	12.081585643914929	17.24566741632491	56.05441118106192	14.618335758698239
16	12.99175894726193	19.292623518517075	48.718706956257826	18.99691057796316
17	13.01230321471094	20.324350404270717	51.44907122790907	15.214275153109266
18	13.664583706216977	18.22074192820401	51.7366909721952	16.377983393383815
19	13.030979821482767	19.64218734192976	49.37908064403166	17.947752192555814
20	15.445242523520852	19.3117670404582	53.53680458821973	11.706185847801219
21	13.48310934375073	23.76489264842066	49.99182898453733	12.760169023291285
22	12.544609853466456	17.594141771009237	52.19208890064824	17.66915947487607
23	13.744737476946064	22.21706886220555	50.75756986218221	13.28062379866618
24	14.619269589036833	19.26647626903652	49.02406947697719	17.090184664949458
25	11.7402706551598	25.442051936530664	47.901761048380195	14.91591635992934
26	12.25014202003066	18.48517155241512	51.705718932631925	17.5589674949223
27	15.945931223395563	19.790510727376017	48.649136596032776	15.614421453195645
28	10.75741422379244	21.570235714341297	51.713656490509955	15.958693571356312
29	15.165560337112751	17.672583519450907	49.313401243550736	17.848454899885606
30	13.429258460891964	22.560251511637862	50.976553076581865	13.033936950888306
31	15.49349042434807	22.22827482626865	45.115211318023704	17.163023431359576
32	19.08718084402699	22.451771553971504	44.56238375757764	13.898663844423867
33	17.061702839622424	21.413040940678428	42.98856836027174	18.536687859427406
34	16.257363641315767	25.068208524314606	40.92729352622118	17.74713430814845
35	15.78157708380349	24.938561745640182	42.133491046901625	17.1463701236547
36	15.436215496914468	27.20636872290919	40.613215255674966	16.744200524501373
37	17.569083990257038	25.49948250235403	42.184384800354856	14.747048707034077
38	19.005159412620717	27.020536485529522	37.106526695874805	16.867777405974955
39	20.821615059570593	25.41574904866034	37.81156860151125	15.951067290257814
40	18.750535006964817	25.590842237146216	39.56608016933457	16.0925425865544
41	15.612398154128698	28.241675291627434	38.94648373967923	17.19944281456464
42	16.704045819941946	25.468666101180514	41.534127607915764	16.29316047096177
43	18.795358863217203	22.63231208609916	39.76000560298203	18.81232344770161
44	17.76954623627464	23.33470813910959	39.95548742052715	18.940258204088618
45	16.559457755850058	23.337042714956073	39.22756667159521	20.875932857598656
46	20.249488338793647	24.5885310070582	38.503848159186944	16.658132494961205
47	14.602149366162658	23.455950444736697	45.46928865473958	16.472611534361064
48	16.52101507357805	25.0221395609441	39.84622927091196	18.610616094565884
49	16.207714994980662	21.806339151615138	44.09749188734893	17.888453966055266
50	16.448487583947458	21.28915278242531	42.396364287215086	19.865995346412145
51	15.961183785592553	20.805273028645246	39.43409881481366	23.79944437094854
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	652.0
1	1558.0
2	2464.0
3	37235.0
4	72006.0
5	45422.5
6	18839.0
7	17749.5
8	16660.0
9	15967.5
10	15275.0
11	14545.0
12	13815.0
13	13130.5
14	12446.0
15	11519.5
16	10593.0
17	9838.5
18	9084.0
19	8310.0
20	7536.0
21	7262.5
22	6989.0
23	7027.0
24	7065.0
25	7198.0
26	8009.0
27	8687.0
28	9206.0
29	9725.0
30	11115.0
31	12505.0
32	13180.0
33	13855.0
34	14351.0
35	14847.0
36	16131.0
37	17415.0
38	18727.5
39	20040.0
40	22025.0
41	24010.0
42	25528.5
43	27047.0
44	56182.5
45	85318.0
46	65028.0
47	44738.0
48	43022.5
49	41307.0
50	36138.0
51	30969.0
52	27966.0
53	24963.0
54	22233.0
55	19503.0
56	16693.0
57	13883.0
58	12006.0
59	10129.0
60	8535.0
61	6941.0
62	5998.5
63	5056.0
64	4369.0
65	3682.0
66	2975.0
67	2268.0
68	1893.0
69	1518.0
70	1270.0
71	1022.0
72	930.0
73	838.0
74	723.0
75	507.5
76	407.0
77	321.5
78	236.0
79	179.5
80	123.0
81	97.0
82	71.0
83	49.0
84	27.0
85	21.5
86	16.0
87	9.0
88	2.0
89	2.5
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	642515.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.44661420462143
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.0057887456186	33.159690602760975
2	8.774941758482328	7.27383251463679
3	3.4075905767942505	4.236992760110842
4	1.775624787795731	2.9437454220772987
5	1.1346132368085178	2.3512938548729707
6	0.7735583545412263	1.9236824811259186
7	0.5793907943997112	1.680965071043578
8	0.4397004830544009	1.4579277029393152
9	0.38343064431015966	1.430271179005735
>10	2.5562545397167646	18.86347684861406
>50	0.10380395448928989	2.934496809183679
>100	0.05624286979406099	3.86098238845618
>500	0.004529627097508268	1.340447226560742
>1k	0.0030197513983388456	3.6314730418916827
>5k	0.0	0.0
>10k+	0.0015098756991694228	12.910722096720198
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44384	6.907854291339502	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCG	13697	2.1317790246142114	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTC	13027	2.027501303471514	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGC	11416	1.7767678575597456	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAGGAATTCGTA	3650	0.5680801226430512	No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	3488	0.5428667035010856	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCT	3401	0.5293261635915114	No Hit
GAACTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT	3197	0.4975759320794067	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAGGAATT	3172	0.49368497233527625	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTC	2806	0.43672132168120587	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCC	2495	0.3883177824642226	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGC	1003	0.15610530493451513	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTAGGAATTCGT	953	0.14832338544625417	No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCT	907	0.14116401951705407	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATG	906	0.14100838112728886	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	759	0.11812953783180159	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTT	757	0.11781826105227114	No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	698	0.10863559605612322	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTAGGAAT	684	0.10645665859941014	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14801210866672374	0.0
2	0.0	0.0	0.0	0.9335190618117865	0.0
3	0.0	0.0	0.0	1.1454985486720155	0.0
4	0.0	0.0	0.0	1.916064216399617	0.0
5	0.0	0.0	0.0	4.64098114440908	0.0
6	0.0	0.0	0.0	5.174665182914017	0.0
7	0.0	0.0	0.0	6.151762993860065	0.0
8	0.0	0.0	0.0	7.20123265604694	0.0
9	0.0	0.0	0.0	7.489630592281892	0.0
10	0.0	0.0	0.0	10.369407718107748	0.0
11	0.0	0.0	0.0	11.538252025244546	0.0
12	0.0	0.0	0.0	14.28464705104161	0.0
13	0.0	0.0	0.0	14.764791483467311	0.0
14	0.0	0.0	0.0	15.004630242095516	0.0
15	0.0	0.0	0.0	15.847256484284413	0.0
16	0.0	0.0	0.0	16.450043967845108	0.0
17	0.0	0.0	0.0	17.037578889208813	0.0
18	1.556383897652195E-4	0.0	0.0	17.637564881753732	0.0
19	1.556383897652195E-4	0.0	0.0	18.540267542392005	0.0
20	1.556383897652195E-4	0.0	0.0	19.015587184734986	0.0
21	1.556383897652195E-4	0.0	0.0	19.459467872345392	0.0
22	1.556383897652195E-4	0.0	0.0	19.956265612475974	0.0
23	1.556383897652195E-4	0.0	0.0	20.38224788526338	0.0
24	1.556383897652195E-4	0.0	0.0	20.763717578577932	0.0
25	1.556383897652195E-4	0.0	0.0	21.08184244725804	0.0
26	1.556383897652195E-4	0.0	0.0	21.42237924406434	0.0
27	1.556383897652195E-4	0.0	0.0	21.79809031695758	0.0
28	4.669151692956585E-4	0.0	0.0	22.13598126113787	0.0
29	4.669151692956585E-4	0.0	0.0	22.491459343361633	0.0
30	4.669151692956585E-4	0.0	0.0	22.907480759204066	0.0
31	6.22553559060878E-4	0.0	0.0	23.253309261262384	0.0
32	6.22553559060878E-4	0.0	0.0	23.605207660521543	0.0
33	6.22553559060878E-4	0.0	0.0	23.98340894765103	0.0
34	6.22553559060878E-4	0.0	0.0	24.347135864532344	0.0
35	6.22553559060878E-4	0.0	0.0	24.726115343610655	0.0
36	6.22553559060878E-4	0.0	0.0	25.08735204625573	0.0
37	6.22553559060878E-4	0.0	0.0	25.44998949440869	0.0
38	6.22553559060878E-4	0.0	0.0	25.84297642856587	0.0
39	6.22553559060878E-4	0.0	0.0	26.26195497381384	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	80	0.0	45.0	31
CGTATGG	20	7.030962E-4	45.0	2
CCGATTT	20	7.030962E-4	45.0	11
TGCGACG	25	3.8888782E-5	45.0	1
CTCGAAT	35	1.2108103E-7	45.0	43
CATCGAA	25	3.8888782E-5	45.0	42
GCTACGA	20	7.030962E-4	45.0	10
CGACTGG	35	1.2108103E-7	45.0	2
TTCGCAG	20	7.030962E-4	45.0	1
CAATCGA	20	7.030962E-4	45.0	41
TCGAATA	35	1.2108103E-7	45.0	44
TCGCGCG	20	7.030962E-4	45.0	1
ACGACTG	20	7.030962E-4	45.0	1
GTACGAG	25	3.8888782E-5	45.0	1
CGGGTCA	30	2.1640008E-6	44.999996	6
CGTGCGG	30	2.1640008E-6	44.999996	2
GCGCCTA	30	2.1640008E-6	44.999996	42
CGTTTTT	18375	0.0	44.130615	1
CCGATGA	960	0.0	42.656246	18
CGATGAA	960	0.0	42.656246	19
>>END_MODULE