FastQCFastQC Report
Sat 18 Jun 2016
SRR3552154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences666644
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT492427.3865511427388535No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCG163992.459933637743683No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTC155622.3343793688985426No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGC130791.9619167051679758No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCT41360.6204210943172068No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC38630.5794697019698669No Hit
GAACTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT35960.5394183402235676No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAGGAATTCGTA34530.5179676108987705No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTC33170.49756691727518737No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCC22920.34381168959744635No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAGGAATT12590.18885642111831802No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCT9700.14550494716820372No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGC9210.13815469725970683No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTT8520.12780434534774182No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATG8440.12660430454635457No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTAGGAATTCGT8170.12255416684167261No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA7670.11505391183300231No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA7220.10830368232519906No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA7150.1072536466239852No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTAT6830.10245348341843623No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG207.0311513E-445.02
ACAATCG207.0311513E-445.01
TATCGAG207.0311513E-445.01
CGGACGG351.2109012E-745.02
GGTAATC302.1641245E-644.9999968
ATCTACC302.1641245E-644.99999615
TAGCACG302.1641245E-644.9999961
TACGAGG551.8189894E-1244.9999962
CGTTTTT203450.044.303271
TACGGCT16400.042.5304877
ACGGCTG16550.042.2809688
ACAACGA1850.041.35135313
CACAACG1850.041.35135312
ATGCTAG556.002665E-1140.909091
AGGGTAC1050.040.7142876
CGGCTGT17200.040.5523269
CGGGCAT501.0804797E-940.5000046
GTACGAG501.0804797E-940.5000041
GGCGACT4100.040.06097410
GAGCGAC1700.039.70588318
Produced by FastQC (version 0.11.5)