##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552154_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	666644
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.721043015462527	31.0	30.0	33.0	28.0	34.0
2	30.7530271029215	31.0	30.0	33.0	26.0	34.0
3	30.376709308116475	31.0	30.0	34.0	25.0	34.0
4	34.49180372132653	35.0	35.0	37.0	32.0	37.0
5	35.1860693263571	35.0	35.0	37.0	33.0	37.0
6	35.14252884598076	36.0	35.0	37.0	33.0	37.0
7	35.6079601706458	37.0	35.0	37.0	35.0	37.0
8	35.111258782798615	37.0	35.0	37.0	32.0	37.0
9	37.1173024882846	39.0	37.0	39.0	33.0	39.0
10	36.81701327845147	39.0	37.0	39.0	32.0	39.0
11	37.06955436484841	39.0	37.0	39.0	33.0	39.0
12	37.30696443679085	39.0	37.0	39.0	34.0	39.0
13	37.15626331295264	39.0	37.0	39.0	33.0	39.0
14	38.39071228421766	40.0	38.0	41.0	34.0	41.0
15	38.38299452181374	40.0	38.0	41.0	33.0	41.0
16	38.34897036499241	40.0	37.0	41.0	34.0	41.0
17	37.92981861383287	40.0	37.0	41.0	33.0	41.0
18	37.5139309736531	39.0	37.0	40.0	33.0	41.0
19	36.72549066668267	37.0	36.0	39.0	32.0	41.0
20	36.06108507689262	36.0	35.0	40.0	32.0	41.0
21	36.33351983967455	37.0	35.0	40.0	33.0	41.0
22	36.634725580669745	37.0	35.0	40.0	33.0	41.0
23	36.67400141604814	37.0	35.0	40.0	33.0	41.0
24	36.50346661786501	37.0	35.0	40.0	33.0	41.0
25	36.11321934945788	36.0	35.0	40.0	32.0	41.0
26	36.23381144958929	37.0	35.0	40.0	33.0	41.0
27	36.44840274569335	37.0	35.0	40.0	33.0	41.0
28	36.237782084590876	37.0	35.0	40.0	33.0	41.0
29	36.16983427436533	37.0	35.0	40.0	33.0	41.0
30	35.709485122494165	36.0	35.0	40.0	31.0	41.0
31	35.36407437852887	37.0	35.0	40.0	29.0	41.0
32	34.7169538764318	37.0	35.0	40.0	22.0	41.0
33	33.80771446229172	37.0	34.0	40.0	16.0	41.0
34	32.98084584875886	37.0	33.0	40.0	12.0	41.0
35	32.45855509087309	37.0	32.0	40.0	10.0	41.0
36	32.09089259034807	37.0	31.0	40.0	8.0	41.0
37	31.71865043411476	37.0	30.0	40.0	8.0	41.0
38	31.635552108771698	36.0	29.0	40.0	8.0	41.0
39	31.583092325139056	36.0	29.0	40.0	7.0	41.0
40	31.45478396265473	36.0	28.0	40.0	7.0	41.0
41	31.218026412898038	36.0	26.0	40.0	7.0	41.0
42	31.13661864503393	36.0	25.0	40.0	7.0	41.0
43	31.14954008436287	36.0	25.0	40.0	7.0	41.0
44	31.137886188130395	36.0	25.0	40.0	7.0	41.0
45	31.0779396499481	36.0	24.0	40.0	7.0	41.0
46	30.958345083732848	35.0	24.0	40.0	7.0	41.0
47	30.937876887814184	35.0	24.0	40.0	7.0	41.0
48	30.93654034237164	35.0	24.0	40.0	7.0	41.0
49	30.929310096543283	35.0	24.0	40.0	7.0	41.0
50	30.804303346313773	35.0	24.0	40.0	7.0	41.0
51	29.776439898956564	35.0	23.0	39.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	12.0
10	24.0
11	16.0
12	14.0
13	13.0
14	22.0
15	52.0
16	105.0
17	228.0
18	528.0
19	1080.0
20	1610.0
21	2489.0
22	3470.0
23	5596.0
24	9283.0
25	16381.0
26	26036.0
27	32478.0
28	31629.0
29	25967.0
30	22319.0
31	21089.0
32	21765.0
33	25877.0
34	36703.0
35	51614.0
36	59332.0
37	73238.0
38	120160.0
39	77509.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.63739567145283	11.79070088383005	14.528143956894535	37.043759487822584
2	50.56536922255357	25.57316948776258	13.802269277155425	10.059192012528426
3	19.63896772490265	24.61238682115192	46.02816495760856	9.720480496336876
4	17.291687917389194	15.108513689465441	55.85979923317392	11.739999159971438
5	22.230905850798926	17.905208777098423	46.366276453399415	13.497608918703236
6	14.819903876731807	28.299662188514407	47.904128740377175	8.976305194376609
7	45.81200760825868	4.802563287151764	45.811707598058334	3.5737215065312222
8	43.326123088185	11.266883074024516	40.65273219289456	4.7542616448959265
9	39.52814395689453	6.128608372684671	40.91269103149507	13.430556638925722
10	24.280275529367998	16.469509963338755	47.27545736555043	11.974757141742819
11	19.78132256496721	16.630315430724647	49.95244838324503	13.635913621063118
12	16.514511493390778	15.20091683117226	53.1445569149351	15.140014760501858
13	15.380022920779307	16.379656908334884	57.17894408409886	11.06137608678695
14	13.887622179154091	21.07961670696804	50.80822747973431	14.22453363414356
15	11.841252602588488	16.8556230911851	58.624543234469975	12.67858107175644
16	12.732132892518347	19.361008274281325	50.300460215647334	17.606398617552994
17	12.694781622575169	18.318472828076153	53.76362796335076	15.223117585997926
18	13.415706134008557	18.235069992379742	53.57942170033782	14.769802173273892
19	12.715782336599442	19.68756937735883	51.09863735367003	16.498010932371702
20	13.766718068414328	19.256004704159942	55.4007836266433	11.576493600782428
21	12.984141460809667	22.71767240086163	51.79091089097029	12.50727524735841
22	12.206515021510732	17.311188580411734	52.438482908418884	18.043813489658646
23	12.215215317320789	22.52221575533568	52.56853733026923	12.694031597074302
24	14.772952280377533	17.506945236138026	50.3198108735697	17.400291609914735
25	11.551292743953294	24.719190452475384	49.30487636579643	14.424640437774885
26	12.144412910038941	17.976311194580617	52.10997173903913	17.769304156341313
27	14.744001296044065	19.683819249854494	49.758341783620644	15.813837670480796
28	10.509207313048643	19.572365460425655	53.7549276675407	16.163499558985006
29	15.383023022782774	17.227935749815494	49.27637539676349	18.112665830638242
30	13.357654160241447	20.901260642861857	52.94895026430899	12.79213493258771
31	15.469125950282308	21.977097221305524	46.46827992151733	16.085496906894832
32	18.289371838642513	22.067850306910437	45.96246272373261	13.680315130714444
33	16.029094989229634	21.150419114249885	44.396709488122596	18.423776408397885
34	16.268953144406908	25.0750025500867	41.939475942182035	16.716568363324352
35	14.883506039205333	25.104103539520345	43.01201240842188	17.000378012852437
36	15.137164663598563	27.03256910734965	41.67486694547615	16.15539928357564
37	17.653950234307967	25.486466539862356	42.79690509477322	14.062678131056455
38	18.770438194898624	27.19847474814144	37.87898788558811	16.152099171371827
39	21.029364998409946	25.07350249908497	38.804969368958545	15.09216313354654
40	18.780488536610246	25.946532182094188	40.44002496084867	14.832954320446895
41	15.287769784172662	28.708276081386767	39.93360774266325	16.07034639177732
42	16.51376146788991	25.46786590744085	42.709452121372124	15.308920503297113
43	18.910092943160066	22.524465831838285	40.53227809745532	18.033163127546338
44	17.677051019734673	23.289041827422132	40.32767114081879	18.70623601202441
45	16.5544128500369	22.773774308326484	40.13476458199578	20.537048259640827
46	20.139534744181304	24.552234775982384	39.15718134416571	16.15104913567061
47	14.030877049819694	22.700421814341688	46.99494782822616	16.27375330761246
48	16.686267333089326	24.221623535200195	40.5898800559219	18.502229075788577
49	15.654982269397161	21.15356922135353	45.42379440900991	17.767654100239408
50	16.234151961166678	20.697703721926544	43.226369696569684	19.84177462033709
51	16.03824530034021	20.50929731610875	39.833704345947766	23.61875303760328
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	793.0
1	1743.0
2	2693.0
3	41903.5
4	81114.0
5	51100.0
6	21086.0
7	19659.5
8	18233.0
9	17548.0
10	16863.0
11	15969.0
12	15075.0
13	14389.5
14	13704.0
15	12758.0
16	11812.0
17	10842.0
18	9872.0
19	8993.0
20	8114.0
21	7745.5
22	7377.0
23	7396.0
24	7415.0
25	7570.5
26	8223.0
27	8720.0
28	9288.5
29	9857.0
30	11185.0
31	12513.0
32	13092.0
33	13671.0
34	14365.5
35	15060.0
36	16371.0
37	17682.0
38	18859.0
39	20036.0
40	22080.5
41	24125.0
42	26033.0
43	27941.0
44	61524.0
45	95107.0
46	68933.0
47	42759.0
48	41922.0
49	41085.0
50	35521.0
51	29957.0
52	26897.5
53	23838.0
54	20937.5
55	18037.0
56	15278.5
57	12520.0
58	10865.0
59	9210.0
60	7884.5
61	6559.0
62	5852.5
63	5146.0
64	4346.0
65	3546.0
66	2910.0
67	2274.0
68	1912.5
69	1551.0
70	1348.0
71	1145.0
72	1003.5
73	862.0
74	756.5
75	519.5
76	388.0
77	311.5
78	235.0
79	181.5
80	128.0
81	106.5
82	85.0
83	60.0
84	35.0
85	31.0
86	27.0
87	16.5
88	6.0
89	4.5
90	3.0
91	2.5
92	2.0
93	3.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	666644.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.71469438750698
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.83833875011887	32.505935626173454
2	8.92340536963665	7.266274450411901
3	3.3641951951994202	4.109165376973914
4	1.837787162352501	2.99299770657863
5	1.1002507184767905	2.2398185876208747
6	0.8142254511336334	1.9890564243261528
7	0.5756014676215213	1.6404806490247514
8	0.48617497657572845	1.5835572472107318
9	0.3812592241230642	1.3970567513329728
>10	2.512133617565505	18.253276078560926
>50	0.09803843784342701	2.800346177083117
>100	0.06001592568373428	4.214861717147789
>500	0.0044732366969242945	1.3715640537922498
>1k	0.0026093880732058383	3.326236339815309
>5k	0.0	0.0
>10k+	0.0014910788989747647	14.309372813947205
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49242	7.3865511427388535	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCG	16399	2.459933637743683	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTC	15562	2.3343793688985426	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGC	13079	1.9619167051679758	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCT	4136	0.6204210943172068	No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	3863	0.5794697019698669	No Hit
GAACTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCT	3596	0.5394183402235676	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAGGAATTCGTA	3453	0.5179676108987705	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTC	3317	0.49756691727518737	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCC	2292	0.34381168959744635	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAGGAATT	1259	0.18885642111831802	No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCT	970	0.14550494716820372	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGC	921	0.13815469725970683	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTT	852	0.12780434534774182	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATG	844	0.12660430454635457	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTAGGAATTCGT	817	0.12255416684167261	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	767	0.11505391183300231	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	722	0.10830368232519906	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	715	0.1072536466239852	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTAT	683	0.10245348341843623	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16050545718554432	0.0
2	0.0	0.0	0.0	0.9886836152429183	0.0
3	0.0	0.0	0.0	1.211441189000426	0.0
4	0.0	0.0	0.0	2.0180186126328294	0.0
5	0.0	0.0	0.0	5.083372834676379	0.0
6	0.0	0.0	0.0	5.628491368706536	0.0
7	0.0	0.0	0.0	6.717078380664943	0.0
8	0.0	0.0	0.0	7.822915979143291	0.0
9	0.0	0.0	0.0	8.108375684773282	0.0
10	0.0	0.0	0.0	11.311134578575672	0.0
11	0.0	0.0	0.0	12.456273513299452	0.0
12	0.0	0.0	0.0	15.384673078884681	0.0
13	0.0	0.0	0.0	15.854039037327269	0.0
14	1.500051001734059E-4	0.0	0.0	16.092097131302463	0.0
15	1.500051001734059E-4	0.0	0.0	16.8742237236066	0.0
16	1.500051001734059E-4	0.0	0.0	17.446343175667973	0.0
17	1.500051001734059E-4	0.0	0.0	18.007662260516856	0.0
18	1.500051001734059E-4	0.0	0.0	18.60888270201187	0.0
19	1.500051001734059E-4	0.0	0.0	19.18940243968295	0.0
20	1.500051001734059E-4	0.0	0.0	19.60701663856571	0.0
21	1.500051001734059E-4	0.0	0.0	20.03903132706512	0.0
22	1.500051001734059E-4	0.0	0.0	20.525497866927477	0.0
23	1.500051001734059E-4	0.0	0.0	20.964862805335382	0.0
24	1.500051001734059E-4	0.0	0.0	21.324125020250687	0.0
25	1.500051001734059E-4	0.0	0.0	21.65503627123322	0.0
26	1.500051001734059E-4	0.0	0.0	21.967796905094772	0.0
27	1.500051001734059E-4	0.0	0.0	22.32405901800661	0.0
28	1.500051001734059E-4	0.0	0.0	22.65422024348828	0.0
29	1.500051001734059E-4	0.0	0.0	22.998931963686765	0.0
30	1.500051001734059E-4	0.0	0.0	23.425996483880454	0.0
31	1.500051001734059E-4	0.0	0.0	23.774908346883795	0.0
32	1.500051001734059E-4	0.0	0.0	24.12562027108922	0.0
33	3.000102003468118E-4	0.0	0.0	24.49253274611337	0.0
34	3.000102003468118E-4	0.0	0.0	24.842194634617577	0.0
35	3.000102003468118E-4	0.0	0.0	25.216907374850745	0.0
36	3.000102003468118E-4	0.0	0.0	25.558168977745243	0.0
37	3.000102003468118E-4	0.0	0.0	25.907080840748584	0.0
38	3.000102003468118E-4	0.0	0.0	26.28644373908713	0.0
39	3.000102003468118E-4	0.0	0.0	26.684857285147697	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	20	7.0311513E-4	45.0	2
ACAATCG	20	7.0311513E-4	45.0	1
TATCGAG	20	7.0311513E-4	45.0	1
CGGACGG	35	1.2109012E-7	45.0	2
GGTAATC	30	2.1641245E-6	44.999996	8
ATCTACC	30	2.1641245E-6	44.999996	15
TAGCACG	30	2.1641245E-6	44.999996	1
TACGAGG	55	1.8189894E-12	44.999996	2
CGTTTTT	20345	0.0	44.30327	1
TACGGCT	1640	0.0	42.530487	7
ACGGCTG	1655	0.0	42.280968	8
ACAACGA	185	0.0	41.351353	13
CACAACG	185	0.0	41.351353	12
ATGCTAG	55	6.002665E-11	40.90909	1
AGGGTAC	105	0.0	40.714287	6
CGGCTGT	1720	0.0	40.552326	9
CGGGCAT	50	1.0804797E-9	40.500004	6
GTACGAG	50	1.0804797E-9	40.500004	1
GGCGACT	410	0.0	40.060974	10
GAGCGAC	170	0.0	39.705883	18
>>END_MODULE