SRR3552152_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552152_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	324275
Sequences flagged as poor quality	0
Sequence length	51
%GC	36

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17342	5.347929997687148	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCG	8477	2.6141392336751212	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGC	7432	2.2918818903708273	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTC	6918	2.1333744506977106	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	2449	0.7552231901935086	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCT	1995	0.6152185644900162	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTA	1916	0.5908565260966772	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCT	1534	0.4730552771567343	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTC	1510	0.4656541515688844	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCC	1403	0.4326574666563873	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTGAGTGT	872	0.2689075630252101	No Hit
CTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGCT	832	0.25657235371212705	TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	730	0.2251175699637653	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGT	564	0.17392645131447074	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGC	543	0.16745046642510214	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTAT	479	0.1477141315241693	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATG	445	0.13722920360804874	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	420	0.12951969778737185	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	414	0.12766941639040938	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTT	403	0.12427723382931154	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	402	0.12396885359648446	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGGA	20	7.0257875E-4	45.000004	4
TTGTACT	20	7.0257875E-4	45.000004	22
CTGCGAG	20	7.0257875E-4	45.000004	1
CGGGCTA	20	7.0257875E-4	45.000004	6
GCGATGC	30	2.1606647E-6	45.000004	9
GGTTGCG	20	7.0257875E-4	45.000004	2
CGATGCA	20	7.0257875E-4	45.000004	10
GCTACAG	30	2.1606647E-6	45.000004	27
TGATCCG	25	3.8845912E-5	45.0	4
ATCCGGC	25	3.8845912E-5	45.0	6
TGCGAAG	25	3.8845912E-5	45.0	1
CGTTTTT	7325	0.0	44.293518	1
GAATGAC	200	0.0	43.875	1
AATGACT	205	0.0	42.80488	2
GACTAAT	145	0.0	41.896553	9
TACGGCT	910	0.0	41.291206	7
ACGGCTG	905	0.0	41.270714	8
CCCTCGT	55	6.002665E-11	40.909092	14
TCGTGTA	55	6.002665E-11	40.909092	17
GCCCTCG	55	6.002665E-11	40.909092	13

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content