##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552152_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	324275
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.625419782591937	31.0	30.0	33.0	28.0	34.0
2	30.562241924292653	31.0	30.0	33.0	26.0	34.0
3	30.269499653072238	31.0	30.0	34.0	25.0	34.0
4	34.48914964150798	35.0	35.0	37.0	32.0	37.0
5	35.16105157659394	35.0	35.0	37.0	33.0	37.0
6	35.110856526096676	35.0	35.0	37.0	33.0	37.0
7	35.598569115719684	37.0	35.0	37.0	35.0	37.0
8	35.00593940328425	37.0	35.0	37.0	32.0	37.0
9	37.01694549379385	39.0	37.0	39.0	32.0	39.0
10	36.757970858068	39.0	35.0	39.0	32.0	39.0
11	37.02061213476216	39.0	37.0	39.0	33.0	39.0
12	37.19270680749364	39.0	37.0	39.0	34.0	39.0
13	37.07345000385475	39.0	37.0	39.0	33.0	39.0
14	38.25827769639966	40.0	38.0	41.0	33.0	41.0
15	38.30459949117262	40.0	38.0	41.0	33.0	41.0
16	38.21068845886979	40.0	37.0	41.0	34.0	41.0
17	37.879950659162745	40.0	37.0	41.0	33.0	41.0
18	37.61227353326652	39.0	37.0	40.0	33.0	41.0
19	36.907307069616834	37.0	36.0	40.0	32.0	41.0
20	36.42653303523244	37.0	35.0	40.0	32.0	41.0
21	36.70265361190348	38.0	35.0	40.0	33.0	41.0
22	37.01694549379385	38.0	35.0	40.0	33.0	41.0
23	37.097608511294425	38.0	35.0	40.0	33.0	41.0
24	37.013497802790845	38.0	35.0	40.0	33.0	41.0
25	36.546884588697864	38.0	35.0	40.0	33.0	41.0
26	36.682411533420705	38.0	35.0	40.0	33.0	41.0
27	36.9157135147637	38.0	35.0	40.0	33.0	41.0
28	36.72041014570966	38.0	35.0	40.0	33.0	41.0
29	36.65211009174312	38.0	35.0	40.0	33.0	41.0
30	36.26008480456403	38.0	35.0	40.0	31.0	41.0
31	35.992817824377454	38.0	35.0	40.0	31.0	41.0
32	35.50362809343921	38.0	35.0	40.0	27.0	41.0
33	34.78714054429111	38.0	35.0	40.0	21.0	41.0
34	34.1887101996762	38.0	34.0	40.0	16.0	41.0
35	33.85199290725465	38.0	34.0	40.0	15.0	41.0
36	33.447049572122424	38.0	33.0	40.0	10.0	41.0
37	33.17872176393493	38.0	33.0	40.0	10.0	41.0
38	33.07340374681983	38.0	33.0	40.0	10.0	41.0
39	33.11409760234369	38.0	33.0	40.0	10.0	41.0
40	32.938934546295584	38.0	33.0	40.0	10.0	41.0
41	32.78063063757613	38.0	32.0	40.0	10.0	41.0
42	32.5869647675584	38.0	32.0	40.0	10.0	41.0
43	32.67003315087503	38.0	32.0	40.0	10.0	41.0
44	32.6671066224655	38.0	32.0	40.0	10.0	41.0
45	32.67001464806106	38.0	32.0	40.0	10.0	41.0
46	32.578604579446456	38.0	31.0	40.0	10.0	41.0
47	32.54980803330506	37.0	31.0	40.0	10.0	41.0
48	32.57852440058592	37.0	31.0	40.0	10.0	41.0
49	32.52329041708426	37.0	31.0	40.0	10.0	41.0
50	32.432722226505284	37.0	31.0	40.0	10.0	41.0
51	31.068383316629404	35.0	27.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	8.0
11	6.0
12	3.0
13	7.0
14	9.0
15	21.0
16	39.0
17	112.0
18	217.0
19	372.0
20	623.0
21	940.0
22	1390.0
23	2135.0
24	3531.0
25	6292.0
26	9929.0
27	12676.0
28	12156.0
29	10300.0
30	9055.0
31	8803.0
32	9595.0
33	12676.0
34	19485.0
35	24254.0
36	28113.0
37	40482.0
38	66948.0
39	44089.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.161899622234216	11.925371983655848	15.543905635648755	32.368822758461185
2	42.429111093978875	28.778043327422715	17.14840798704803	11.644437591550382
3	22.225888520545833	26.711279007015648	40.56186878421093	10.500963688227586
4	20.235910878112712	17.038316243928765	50.2517924601033	12.473980417855216
5	24.75491480996068	20.489707809729396	40.11194202451623	14.643435355793693
6	16.319173540976024	32.39349317708735	41.3155500732403	9.971783208696323
7	51.781358414925606	5.615295659548223	38.51607431963611	4.087271605890062
8	49.87556857605428	12.842803176316398	32.099915195435976	5.181713052193354
9	45.38740266748901	7.220723151645979	33.030915118340914	14.360959062524092
10	28.63402975869247	19.165523089969934	39.39773340528872	12.802713746048877
11	23.337907640120267	19.340374681982887	42.69894379770257	14.622773880194279
12	20.18965384318865	16.80888135070542	46.41770102536427	16.583763780741652
13	17.476524554776038	20.236527638578366	50.191658314702025	12.095289491943566
14	14.898157428108858	24.53781512605042	43.591087811271294	16.97293963456942
15	12.756765091357645	19.83902551846427	53.431809421016105	13.972399969161978
16	13.902089276077403	21.61683756071236	44.71790918202143	19.763163981188807
17	13.840721609744817	21.03893300439442	47.93153958831239	17.188805797548376
18	13.532341376917739	21.456479839642277	47.902243466193816	17.108935317246164
19	15.013800015419013	22.58145092899545	44.68429573664328	17.720453318942255
20	15.714748284634956	21.642741500269832	49.87865237838254	12.763857836712667
21	15.042170996839102	26.599953742965077	44.786986354174694	13.570888906021125
22	13.165368899853519	20.797471282090818	45.73864775267905	20.29851206537661
23	13.751599722457792	25.22241924292653	45.41669878960759	15.609282245008094
24	16.11595096754298	20.657466656387324	44.02436203839334	19.202220337676355
25	12.218949965307225	28.69046334129982	42.803176316398115	16.287410376994835
26	13.30784056741963	23.063757613137	45.09536658700177	18.5330352324416
27	16.92051499498882	24.108858222187955	42.099452625086734	16.87117415773649
28	11.845193123120808	22.85035849202066	48.69848122735333	16.6059671575052
29	17.48670110245933	22.5740498034076	42.38871328347853	17.550535810654537
30	16.587155963302752	25.436435124508517	44.00616760465654	13.970241307532188
31	18.6958599953743	22.541978259193584	42.09575206229281	16.66640968313931
32	20.434816128286176	25.69979184334284	40.098064914039014	13.76732711433197
33	17.193431501040784	24.86377303214864	38.233289646133684	19.709505820676895
34	20.493716752756146	25.963765322642818	37.92398427260812	15.618533651992909
35	19.99506591627477	24.583763780741656	37.335286408141236	18.085883894842343
36	20.527021817901474	28.109783362886436	37.053118495104464	14.310076324107623
37	23.340066301750056	24.81443219489631	38.31192660550459	13.533574897849048
38	17.810191966694937	28.399352401511063	36.916197671729236	16.87425796006476
39	25.563487780433274	25.024747513684375	34.99190501888829	14.419859686994062
40	18.776655616374992	26.431269755608668	39.5318788065685	15.260195821447844
41	22.232981265900857	26.02636650990672	34.7563025210084	16.984349703184026
42	18.545370441754685	23.851669108010178	41.2766941639041	16.326266286331045
43	23.066224654999615	23.687919204379	35.034153110785596	18.21170302983579
44	20.096523012874876	23.6583147020276	37.376917739572896	18.868244545524632
45	17.913807724924833	23.172307455092128	37.81327576902321	21.100609050959836
46	22.366818286947808	25.91627476678745	36.00986816745046	15.707038778814278
47	15.923213322026058	22.875337290879656	44.779585228586846	16.42186415850744
48	18.838023282707578	24.212165600185028	37.658160511911184	19.291650605196207
49	17.58908333975792	21.01765476832935	43.85136072777735	17.54190116413538
50	18.9749441060828	21.305373525557012	39.40328424947961	20.31639811888058
51	17.695166139850436	21.381851823298128	36.68152031454784	24.2414617223036
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	397.0
1	836.5
2	1276.0
3	14648.5
4	28021.0
5	18107.0
6	8193.0
7	7747.5
8	7302.0
9	7040.0
10	6778.0
11	6397.0
12	6016.0
13	5660.0
14	5304.0
15	4900.5
16	4497.0
17	4066.0
18	3635.0
19	3350.0
20	3065.0
21	2928.5
22	2792.0
23	2794.5
24	2797.0
25	2979.0
26	3351.0
27	3541.0
28	4144.0
29	4747.0
30	5399.0
31	6051.0
32	6541.0
33	7031.0
34	7632.0
35	8233.0
36	8902.5
37	9572.0
38	9829.5
39	10087.0
40	11433.5
41	12780.0
42	14197.5
43	15615.0
44	16737.5
45	17860.0
46	34714.0
47	51568.0
48	38721.5
49	25875.0
50	24354.5
51	22834.0
52	18873.0
53	14912.0
54	12373.0
55	9834.0
56	8107.0
57	6380.0
58	5360.5
59	4341.0
60	3795.5
61	3250.0
62	2741.0
63	2232.0
64	1841.5
65	1451.0
66	1191.0
67	931.0
68	790.5
69	650.0
70	536.0
71	422.0
72	386.5
73	351.0
74	279.5
75	172.5
76	137.0
77	103.0
78	69.0
79	55.0
80	41.0
81	28.5
82	16.0
83	13.5
84	11.0
85	7.0
86	3.0
87	3.0
88	3.0
89	2.5
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	324275.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.09266937584204
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.34063097127158	33.81751616920269
2	8.526042994451007	7.177678176992809
3	3.108782543266762	3.925708672653515
4	1.6625965693339462	2.7993251079353216
5	1.0279269286146113	2.1634094174349814
6	0.7386793629677751	1.8655791720096107
7	0.6017565681880891	1.7730678188637807
8	0.4844002289845433	1.6311758947382848
9	0.47183489158247327	1.7874711082230592
>10	2.917919388877213	20.97144249947137
>50	0.07298472650455574	2.082215996631042
>100	0.03538653406281491	3.0879480450783157
>500	0.0036860972982098866	1.0988266533637137
>1k	0.004423316757851864	3.3535779844398905
>5k	0.002211658378925932	7.083568488091921
>10k+	7.372194596419773E-4	5.381488794869676
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17342	5.347929997687148	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCG	8477	2.6141392336751212	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGC	7432	2.2918818903708273	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTC	6918	2.1333744506977106	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	2449	0.7552231901935086	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCT	1995	0.6152185644900162	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTA	1916	0.5908565260966772	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCT	1534	0.4730552771567343	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTC	1510	0.4656541515688844	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCC	1403	0.4326574666563873	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTGAGTGT	872	0.2689075630252101	No Hit
CTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGCT	832	0.25657235371212705	TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTTCTGC	730	0.2251175699637653	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGT	564	0.17392645131447074	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGC	543	0.16745046642510214	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTAT	479	0.1477141315241693	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATG	445	0.13722920360804874	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	420	0.12951969778737185	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	414	0.12766941639040938	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTGAGTGTCGTATGCCGTCTT	403	0.12427723382931154	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	402	0.12396885359648446	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.083802328270758E-4	0.0	0.0	0.2790841107085036	0.0
2	3.083802328270758E-4	0.0	0.0	1.4879346233906408	0.0
3	3.083802328270758E-4	0.0	0.0	1.7919975329581375	0.0
4	3.083802328270758E-4	0.0	0.0	2.608280009251407	0.0
5	3.083802328270758E-4	0.0	0.0	5.589391719990749	0.0
6	3.083802328270758E-4	0.0	0.0	6.203685143782284	0.0
7	3.083802328270758E-4	0.0	0.0	7.349009328502043	0.0
8	3.083802328270758E-4	0.0	0.0	8.555392799321563	0.0
9	3.083802328270758E-4	0.0	0.0	8.883509367049571	0.0
10	3.083802328270758E-4	0.0	0.0	12.341376917739574	0.0
11	3.083802328270758E-4	0.0	0.0	13.710893531724617	0.0
12	3.083802328270758E-4	0.0	0.0	17.19497340220492	0.0
13	3.083802328270758E-4	0.0	0.0	17.750982961992136	0.0
14	3.083802328270758E-4	0.0	0.0	18.104695089044792	0.0
15	3.083802328270758E-4	0.0	0.0	19.037545293346696	0.0
16	3.083802328270758E-4	0.0	0.0	19.7619304602575	0.0
17	3.083802328270758E-4	0.0	0.0	20.50204301904248	0.0
18	3.083802328270758E-4	0.0	0.0	21.24678128131987	0.0
19	3.083802328270758E-4	0.0	0.0	22.03345925526174	0.0
20	3.083802328270758E-4	0.0	0.0	22.55431346850667	0.0
21	3.083802328270758E-4	0.0	0.0	23.06869169686223	0.0
22	3.083802328270758E-4	0.0	0.0	23.590162670572816	0.0
23	3.083802328270758E-4	0.0	0.0	24.099606815203146	0.0
24	3.083802328270758E-4	0.0	0.0	24.50851900393185	0.0
25	3.083802328270758E-4	0.0	0.0	24.873024439133452	0.0
26	3.083802328270758E-4	0.0	0.0	25.252332125510755	0.0
27	3.083802328270758E-4	0.0	0.0	25.617454321178013	0.0
28	3.083802328270758E-4	0.0	0.0	25.98905250173464	0.0
29	3.083802328270758E-4	0.0	0.0	26.356641739264514	0.0
30	3.083802328270758E-4	0.0	0.0	26.787757304756767	0.0
31	3.083802328270758E-4	0.0	0.0	27.16336442834014	0.0
32	3.083802328270758E-4	0.0	0.0	27.549764860072468	0.0
33	3.083802328270758E-4	0.0	0.0	27.919512759232134	0.0
34	3.083802328270758E-4	0.0	0.0	28.299128825842264	0.0
35	3.083802328270758E-4	0.0	0.0	28.681828694780666	0.0
36	3.083802328270758E-4	0.0	0.0	29.08704032071544	0.0
37	3.083802328270758E-4	0.0	0.0	29.459255261737724	0.0
38	3.083802328270758E-4	0.0	0.0	29.85583224115334	0.0
39	3.083802328270758E-4	0.0	0.0	30.216945493793848	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGGA	20	7.0257875E-4	45.000004	4
TTGTACT	20	7.0257875E-4	45.000004	22
CTGCGAG	20	7.0257875E-4	45.000004	1
CGGGCTA	20	7.0257875E-4	45.000004	6
GCGATGC	30	2.1606647E-6	45.000004	9
GGTTGCG	20	7.0257875E-4	45.000004	2
CGATGCA	20	7.0257875E-4	45.000004	10
GCTACAG	30	2.1606647E-6	45.000004	27
TGATCCG	25	3.8845912E-5	45.0	4
ATCCGGC	25	3.8845912E-5	45.0	6
TGCGAAG	25	3.8845912E-5	45.0	1
CGTTTTT	7325	0.0	44.293518	1
GAATGAC	200	0.0	43.875	1
AATGACT	205	0.0	42.80488	2
GACTAAT	145	0.0	41.896553	9
TACGGCT	910	0.0	41.291206	7
ACGGCTG	905	0.0	41.270714	8
CCCTCGT	55	6.002665E-11	40.909092	14
TCGTGTA	55	6.002665E-11	40.909092	17
GCCCTCG	55	6.002665E-11	40.909092	13
>>END_MODULE