SRR3552151_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552151_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	432807
Sequences flagged as poor quality	0
Sequence length	51
%GC	38

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15735	3.635569665000797	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCG	8917	2.0602716684341984	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC	7709	1.7811634285027738	RNA PCR Primer, Index 27 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGC	6454	1.491195844799183	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTA	2342	0.5411187896683741	No Hit
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	2286	0.5281799970887717	Illumina PCR Primer Index 6 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT	2065	0.47711797637284054	No Hit
GAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	1989	0.4595581864433801	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCCTATTT	1877	0.4336806012841751	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTC	1876	0.4334495514166823	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCC	1298	0.2999027280057855	No Hit
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	787	0.1818362457169131	RNA PCR Primer, Index 27 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	725	0.16751115393235322	RNA PCR Primer, Index 27 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGT	573	0.13239157407343227	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT	536	0.12384272897619493	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATG	512	0.1182975321563653	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGC	480	0.11090393639659248	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTAT	453	0.10466558997428416	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACGCG	30	2.1623528E-6	45.000004	1
TAACGGG	30	2.1623528E-6	45.000004	3
GTTACGG	30	2.1623528E-6	45.000004	2
GATACCG	20	7.0284074E-4	45.0	27
CCGGGTG	25	3.8867624E-5	45.0	5
TTCCCGA	20	7.0284074E-4	45.0	32
ACGATCC	45	3.8380676E-10	45.0	26
GTTCCCG	20	7.0284074E-4	45.0	31
GCTAGCG	20	7.0284074E-4	45.0	1
TCCCGAT	20	7.0284074E-4	45.0	33
CGGCTAT	20	7.0284074E-4	45.0	31
CACAACG	40	6.7975634E-9	45.0	12
TTCGAAT	20	7.0284074E-4	45.0	43
TTCCTCG	20	7.0284074E-4	45.0	12
CGTTTTT	7830	0.0	43.936783	1
ATGGGCG	70	0.0	41.785713	5
CCGATGA	500	0.0	41.399998	18
CGATGAA	505	0.0	40.9901	19
TACGGGC	55	6.002665E-11	40.909092	4
TGACCCA	55	6.002665E-11	40.909092	11

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content