##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552151_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	432807
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.781595491754985	30.0	28.0	33.0	25.0	33.0
2	28.416388829201008	31.0	27.0	33.0	16.0	34.0
3	28.160212288618254	31.0	25.0	33.0	16.0	34.0
4	33.344964383662926	35.0	32.0	35.0	28.0	37.0
5	34.319849263066445	35.0	33.0	35.0	32.0	37.0
6	33.51805077089788	35.0	33.0	37.0	28.0	37.0
7	34.64767667805742	35.0	35.0	37.0	32.0	37.0
8	33.91767231121493	35.0	35.0	37.0	28.0	37.0
9	36.16255975527198	39.0	35.0	39.0	31.0	39.0
10	35.90343963937737	37.0	35.0	39.0	31.0	39.0
11	36.46456734757063	37.0	35.0	39.0	32.0	39.0
12	36.51150281765313	39.0	35.0	39.0	32.0	39.0
13	36.52231364095313	38.0	35.0	39.0	32.0	39.0
14	37.30956061246699	39.0	36.0	40.0	32.0	41.0
15	37.62045900366676	39.0	36.0	41.0	32.0	41.0
16	37.15075541754177	39.0	36.0	41.0	32.0	41.0
17	37.04783656456573	38.0	36.0	40.0	32.0	41.0
18	36.814778873724315	39.0	36.0	40.0	32.0	41.0
19	36.06256830411708	37.0	34.0	40.0	30.0	41.0
20	35.98578350165316	37.0	35.0	39.0	31.0	41.0
21	36.34153098263198	38.0	35.0	40.0	32.0	41.0
22	36.797369266208726	38.0	35.0	40.0	32.0	41.0
23	36.995187231260125	38.0	35.0	40.0	33.0	41.0
24	36.95219578241572	38.0	35.0	40.0	33.0	41.0
25	36.22111010219336	38.0	35.0	40.0	31.0	41.0
26	36.52211031706973	38.0	35.0	40.0	32.0	41.0
27	36.68850087914475	38.0	35.0	40.0	33.0	41.0
28	36.560721060426474	38.0	35.0	40.0	32.0	41.0
29	36.54872957230359	38.0	35.0	40.0	31.0	41.0
30	36.05710859574822	38.0	35.0	40.0	30.0	41.0
31	35.976285041600526	38.0	35.0	40.0	30.0	41.0
32	35.47891554434194	38.0	35.0	40.0	27.0	41.0
33	35.007601540640515	38.0	35.0	40.0	22.0	41.0
34	34.516953284027295	39.0	35.0	40.0	18.0	41.0
35	34.244845855080904	39.0	34.0	40.0	15.0	41.0
36	33.99503704884625	39.0	34.0	40.0	12.0	41.0
37	34.02843530719235	39.0	34.0	40.0	12.0	41.0
38	33.89201653392852	38.0	34.0	40.0	12.0	41.0
39	33.72088482857255	38.0	34.0	40.0	11.0	41.0
40	33.576885309156275	38.0	33.0	40.0	10.0	41.0
41	33.33801209315006	38.0	33.0	40.0	10.0	41.0
42	33.52443698923539	38.0	33.0	40.0	10.0	41.0
43	33.49436584898118	38.0	33.0	40.0	10.0	41.0
44	33.53275016346778	38.0	34.0	40.0	10.0	41.0
45	33.563544489807235	38.0	34.0	40.0	10.0	41.0
46	33.412747483289316	38.0	33.0	40.0	10.0	41.0
47	33.232242084809165	38.0	33.0	40.0	10.0	41.0
48	33.29260848368904	38.0	33.0	40.0	10.0	41.0
49	33.35648453005612	38.0	33.0	40.0	10.0	41.0
50	33.270436938404416	38.0	33.0	40.0	10.0	41.0
51	32.47481671969261	37.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	11.0
11	10.0
12	12.0
13	17.0
14	23.0
15	40.0
16	64.0
17	111.0
18	260.0
19	588.0
20	1212.0
21	2004.0
22	2787.0
23	3702.0
24	5158.0
25	8456.0
26	12333.0
27	14327.0
28	13535.0
29	11640.0
30	10911.0
31	11924.0
32	14858.0
33	20539.0
34	27177.0
35	34968.0
36	47822.0
37	74010.0
38	88729.0
39	25570.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.900678593460825	13.41868315438521	16.55888190348123	29.121756348672733
2	39.703840279847604	27.995619294512338	18.549838611667557	13.75070181397251
3	23.546523046069034	26.740787464158387	36.56733832863147	13.14535116114111
4	21.44050350387124	18.240693888962053	45.64135977468017	14.677442832486538
5	26.247958096796033	21.613559854623425	36.13227142814234	16.00621062043821
6	18.59096548808129	32.267731344456074	37.55068656468126	11.590616602781378
7	57.32116162631381	5.268630128440622	33.385088503651744	4.025119741593828
8	55.91961313010187	11.7364090691694	26.618562084254645	5.725415716474087
9	50.57404339578612	7.543547123775725	27.89326420321298	13.989145277225184
10	30.330378205528095	20.90354361181774	35.3034955534453	13.462582629208864
11	24.26000503688711	21.468691587705376	39.45407537308777	14.817228002319741
12	19.780410205934746	19.130929028412204	42.97920320142696	18.109457564226087
13	19.152647715956537	21.258667258154325	46.09652801364118	13.492157012247954
14	15.049202069282613	26.497491953688364	41.38172441758105	17.071581559447978
15	13.415679506107804	21.726543239827453	48.614740519446315	16.243036734618432
16	14.79481616517293	22.533600426980154	41.55501181820072	21.116571589646192
17	15.041808473522842	23.345971761085195	43.3980966111916	18.214123154200372
18	14.883539314290203	21.813417990004783	44.59747647334724	18.705566222357774
19	15.445221542165447	23.71495839947136	40.80317554937882	20.036644508984374
20	17.30910082323068	23.175688008742927	45.328749303962276	14.186461864064121
21	15.777933351355223	27.231768432580804	41.92908155367173	15.061216662392244
22	15.05682671490988	20.86611353328389	43.34818983981313	20.728869911993105
23	15.734958076001544	24.983653221874878	42.75392958062139	16.527459121502194
24	15.940130358335239	23.31709052764858	41.978988325050196	18.763790788965984
25	13.673300108362389	28.901796874819492	40.35909770405747	17.065805312760656
26	14.996753749361725	23.013953101497897	42.97943425129446	19.009858897845923
27	17.505031110864657	23.66967262544275	40.11765059252739	18.707645671165206
28	13.360227537909509	24.278257976419052	43.358818133717804	19.002696351953645
29	18.35344622429859	20.576608049315283	41.07026919620061	19.99967653018551
30	14.565845746487465	25.419182222098996	41.33227974593757	18.682692285475973
31	17.005270247477515	22.81917806320138	37.96472792722854	22.21082376209257
32	18.403121945809563	23.847118923677296	38.34041501177199	19.40934411874115
33	15.46878862864972	23.346895960555166	38.87945435263293	22.304861058162185
34	18.79475147121003	25.157633772097032	36.320346020281555	19.72726873641138
35	16.510823531042703	23.549064594611455	37.76001774462983	22.180094129716014
36	17.51565940476933	25.6631708821715	36.485546675538984	20.335623037520186
37	15.292035480017653	25.927722980450866	36.471914733356904	22.308326806174577
38	15.635375583112104	25.106802801248595	36.46382798799465	22.793993627644653
39	17.683170558701686	27.132416989558855	35.46037841347298	19.72403403826648
40	16.721772060063724	24.899782119974954	39.81497526611169	18.563470553849637
41	15.737499624543966	27.845436880641948	36.06896376444928	20.348099730364805
42	16.475935001051276	24.02733782032176	39.72486581778945	19.77186136083751
43	18.741841051554157	23.464731392976546	36.94949480946473	20.84393274600457
44	17.725452684452886	24.516008290069248	35.379510959850464	22.379028065627406
45	16.08107077750591	24.142631704200717	36.37210119059996	23.404196327693406
46	19.89385569087376	26.111638674975218	35.211306656315635	18.783198977835387
47	14.783725771533271	24.42243309373462	41.48246215980795	19.311378974924157
48	17.26728079721446	25.331845372186677	36.17825035177342	21.222623478825433
49	17.04246927614387	22.401208852906723	40.45174870092212	20.104573170027287
50	17.03276518170917	22.876709480207115	38.509543514776794	21.580981823306924
51	17.258963001984718	22.28845651757019	35.557419357819995	24.895161122625094
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	434.0
1	935.5
2	1437.0
3	13915.0
4	26393.0
5	17579.0
6	8765.0
7	8351.5
8	7938.0
9	7662.0
10	7386.0
11	6948.0
12	6510.0
13	6028.5
14	5547.0
15	5171.5
16	4796.0
17	4582.5
18	4369.0
19	4038.5
20	3708.0
21	3601.5
22	3495.0
23	3574.0
24	3653.0
25	4016.5
26	4902.0
27	5424.0
28	6208.5
29	6993.0
30	7937.0
31	8881.0
32	9976.5
33	11072.0
34	11860.0
35	12648.0
36	13939.0
37	15230.0
38	16212.0
39	17194.0
40	18561.0
41	19928.0
42	21562.0
43	23196.0
44	24101.5
45	25007.0
46	43534.0
47	62061.0
48	48811.0
49	35561.0
50	33419.0
51	31277.0
52	26686.0
53	22095.0
54	18937.0
55	15779.0
56	13319.5
57	10860.0
58	8991.0
59	7122.0
60	5905.5
61	4689.0
62	3871.5
63	3054.0
64	2551.0
65	2048.0
66	1641.5
67	1235.0
68	1072.5
69	910.0
70	760.0
71	610.0
72	514.0
73	418.0
74	362.0
75	256.5
76	207.0
77	154.0
78	101.0
79	81.0
80	61.0
81	41.0
82	21.0
83	13.5
84	6.0
85	3.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	432807.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.18734307786046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.42950647840806	32.71490333831333
2	8.580742370686389	7.06835959768388
3	3.1929544252164996	3.9452792803009418
4	1.6618621899193915	2.7379075265733785
5	1.1003649571231606	2.266055449994342
6	0.7828808256736788	1.9346868693660286
7	0.6193311491253467	1.7856023162481847
8	0.48164068724058096	1.5870000180507438
9	0.43981169574051043	1.6303207681907985
>10	3.6071436398848395	27.165317932540155
>50	0.06315922320616754	1.7647444463133772
>100	0.03157961160308377	2.4671118068887457
>500	0.002819608178846765	0.7276841735061609
>1k	0.003947451450385471	3.189686164940985
>5k	0.0016917649073080589	5.360660939841108
>10k+	5.63921635769353E-4	3.6546793712478265
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15735	3.635569665000797	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCG	8917	2.0602716684341984	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC	7709	1.7811634285027738	RNA PCR Primer, Index 27 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGC	6454	1.491195844799183	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTA	2342	0.5411187896683741	No Hit
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	2286	0.5281799970887717	Illumina PCR Primer Index 6 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT	2065	0.47711797637284054	No Hit
GAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	1989	0.4595581864433801	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCCTATTT	1877	0.4336806012841751	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTC	1876	0.4334495514166823	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCC	1298	0.2999027280057855	No Hit
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	787	0.1818362457169131	RNA PCR Primer, Index 27 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	725	0.16751115393235322	RNA PCR Primer, Index 27 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGT	573	0.13239157407343227	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT	536	0.12384272897619493	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATG	512	0.1182975321563653	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGC	480	0.11090393639659248	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTAT	453	0.10466558997428416	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18946089134417882	0.0
2	0.0	0.0	0.0	1.0279408604759166	0.0
3	0.0	0.0	0.0	1.2633806754511827	0.0
4	0.0	0.0	0.0	2.042018728902259	0.0
5	0.0	0.0	0.0	4.602282310591095	0.0
6	0.0	0.0	0.0	5.19284577190295	0.0
7	0.0	0.0	0.0	6.133911882201535	0.0
8	0.0	0.0	0.0	7.255890038747062	0.0
9	0.0	0.0	0.0	7.53684667761843	0.0
10	0.0	0.0	0.0	10.382918945395986	0.0
11	0.0	0.0	0.0	11.540478781535418	0.0
12	0.0	0.0	0.0	14.031196353108891	0.0
13	0.0	0.0	0.0	14.541123410665724	0.0
14	0.0	0.0	0.0	14.804751309475124	0.0
15	0.0	0.0	0.0	15.661946317873786	0.0
16	0.0	0.0	0.0	16.301954450829122	0.0
17	0.0	0.0	0.0	16.93248953921725	0.0
18	0.0	0.0	0.0	17.583819115679738	0.0
19	0.0	0.0	0.0	18.4442488222233	0.0
20	0.0	0.0	0.0	18.928760394355916	0.0
21	2.31049867492901E-4	0.0	0.0	19.40310577231884	0.0
22	2.31049867492901E-4	0.0	0.0	19.99551763257064	0.0
23	2.31049867492901E-4	0.0	0.0	20.51353143548972	0.0
24	2.31049867492901E-4	0.0	0.0	20.931038546049393	0.0
25	2.31049867492901E-4	0.0	0.0	21.305570381255386	0.0
26	2.31049867492901E-4	0.0	0.0	21.68726476235366	0.0
27	2.31049867492901E-4	0.0	0.0	22.105002922780823	0.0
28	2.31049867492901E-4	0.0	0.0	22.504026043941064	0.0
29	2.31049867492901E-4	0.0	0.0	22.91968475556079	0.0
30	2.31049867492901E-4	0.0	0.0	23.359372653399785	0.0
31	2.31049867492901E-4	0.0	0.0	23.76994826793467	0.0
32	2.31049867492901E-4	0.0	0.0	24.16573669095001	0.0
33	2.31049867492901E-4	0.0	0.0	24.543965324035888	0.0
34	2.31049867492901E-4	0.0	0.0	24.928663353411565	0.0
35	2.31049867492901E-4	0.0	0.0	25.33276957165665	0.0
36	2.31049867492901E-4	0.0	0.0	25.734565291226804	0.0
37	2.31049867492901E-4	0.0	0.0	26.14583405536417	0.0
38	4.62099734985802E-4	0.0	0.0	26.54670557546435	0.0
39	4.62099734985802E-4	0.0	0.0	26.981541426085993	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACGCG	30	2.1623528E-6	45.000004	1
TAACGGG	30	2.1623528E-6	45.000004	3
GTTACGG	30	2.1623528E-6	45.000004	2
GATACCG	20	7.0284074E-4	45.0	27
CCGGGTG	25	3.8867624E-5	45.0	5
TTCCCGA	20	7.0284074E-4	45.0	32
ACGATCC	45	3.8380676E-10	45.0	26
GTTCCCG	20	7.0284074E-4	45.0	31
GCTAGCG	20	7.0284074E-4	45.0	1
TCCCGAT	20	7.0284074E-4	45.0	33
CGGCTAT	20	7.0284074E-4	45.0	31
CACAACG	40	6.7975634E-9	45.0	12
TTCGAAT	20	7.0284074E-4	45.0	43
TTCCTCG	20	7.0284074E-4	45.0	12
CGTTTTT	7830	0.0	43.936783	1
ATGGGCG	70	0.0	41.785713	5
CCGATGA	500	0.0	41.399998	18
CGATGAA	505	0.0	40.9901	19
TACGGGC	55	6.002665E-11	40.909092	4
TGACCCA	55	6.002665E-11	40.909092	11
>>END_MODULE