FastQCFastQC Report
Sat 18 Jun 2016
SRR3552150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443882
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171063.8537268913810427No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCG109702.471377528262016No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC91962.0717217638922056RNA PCR Primer, Index 27 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGC75371.697973785825963No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT26670.6008353571444663No Hit
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC24110.5431623719817429Illumina PCR Primer Index 6 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTC22510.5071167562550408No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTA22350.5035121946823705No Hit
GAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT20830.4692688597420035No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCC12310.27732595599731463No Hit
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT7560.17031553430866764RNA PCR Primer, Index 27 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC7210.16243055586845154RNA PCR Primer, Index 27 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT7100.15995241978724073No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCCTATTT6960.15679842841115432No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGT5360.12075281268445216No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTAT5290.11917581699640895No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATG4740.1067851365903551No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGC4690.10565871109889566No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGG253.8869224E-545.0000042
TAGGACG253.8869224E-545.0000041
TATACGG253.8869224E-545.0000042
CGATCCA253.8869224E-545.00000427
GCGTAAG253.8869224E-545.00000415
TCACGAC207.0286024E-445.025
TAGCGAG207.0286024E-445.01
GCGTTCT207.0286024E-445.026
GTGTACG207.0286024E-445.01
GTCAAGC406.7993824E-945.016
CCGACAC207.0286024E-445.045
GTATACG207.0286024E-445.01
TCGACAG207.0286024E-445.01
CCGTTGT207.0286024E-445.016
TCGCGTT207.0286024E-445.032
GCCCGTT207.0286024E-445.014
CACGTGA302.16248E-644.99999643
CGTTTTT84800.043.5406841
AACGGGA900.042.54
TGCGAGG700.041.7857132
Produced by FastQC (version 0.11.5)