##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552150_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	443882
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.504895445185884	31.0	30.0	33.0	28.0	34.0
2	30.450468818289547	31.0	30.0	33.0	25.0	34.0
3	30.06369936154203	31.0	30.0	34.0	25.0	34.0
4	34.44282264205352	35.0	33.0	37.0	32.0	37.0
5	35.09654592887299	35.0	35.0	37.0	33.0	37.0
6	35.048769718078226	35.0	35.0	37.0	32.0	37.0
7	35.54880351084297	37.0	35.0	37.0	33.0	37.0
8	35.023943300246465	37.0	35.0	37.0	32.0	37.0
9	37.00178425797847	39.0	37.0	39.0	32.0	39.0
10	36.583152729779535	39.0	35.0	39.0	32.0	39.0
11	36.87741561946643	39.0	37.0	39.0	33.0	39.0
12	37.112775467353934	39.0	37.0	39.0	34.0	39.0
13	37.012474035892424	39.0	37.0	39.0	33.0	39.0
14	38.15780545280052	40.0	37.0	41.0	33.0	41.0
15	38.27264453165481	40.0	38.0	41.0	33.0	41.0
16	38.11186756840782	40.0	37.0	41.0	34.0	41.0
17	37.82815703272491	39.0	37.0	41.0	33.0	41.0
18	37.571108988424854	39.0	37.0	40.0	33.0	41.0
19	36.91695540706765	37.0	36.0	40.0	32.0	41.0
20	36.58022402350174	38.0	35.0	40.0	32.0	41.0
21	36.8340932950649	38.0	35.0	40.0	33.0	41.0
22	37.14901482826517	38.0	35.0	40.0	33.0	41.0
23	37.20772637773102	38.0	35.0	40.0	33.0	41.0
24	37.12724778206821	39.0	35.0	41.0	33.0	41.0
25	36.66977485007277	38.0	35.0	40.0	32.0	41.0
26	36.83289252549101	38.0	35.0	40.0	33.0	41.0
27	37.04061665037104	38.0	35.0	40.0	33.0	41.0
28	36.90612820524374	39.0	35.0	40.0	33.0	41.0
29	36.87314196115184	39.0	35.0	41.0	33.0	41.0
30	36.517000013517105	38.0	35.0	40.0	32.0	41.0
31	36.38712540720282	39.0	35.0	40.0	31.0	41.0
32	35.931668776837085	39.0	35.0	41.0	30.0	41.0
33	35.258449317611436	39.0	35.0	41.0	22.0	41.0
34	34.744770006443154	39.0	35.0	41.0	18.0	41.0
35	34.456218093997954	39.0	35.0	41.0	15.0	41.0
36	34.22068702943575	39.0	35.0	41.0	15.0	41.0
37	33.90926642666294	39.0	34.0	41.0	10.0	41.0
38	33.81447772155663	39.0	34.0	41.0	10.0	41.0
39	33.72127502354229	38.0	34.0	40.0	10.0	41.0
40	33.60131070870186	38.0	33.0	40.0	10.0	41.0
41	33.40148057366597	38.0	33.0	40.0	10.0	41.0
42	33.394613883870036	38.0	33.0	40.0	10.0	41.0
43	33.411872975250176	38.0	33.0	40.0	10.0	41.0
44	33.38985360974313	38.0	33.0	40.0	10.0	41.0
45	33.355795008583364	38.0	33.0	40.0	10.0	41.0
46	33.237259001266104	38.0	33.0	40.0	10.0	41.0
47	33.216859435615774	38.0	33.0	40.0	10.0	41.0
48	33.201078214480425	38.0	33.0	40.0	10.0	41.0
49	33.177060570151525	38.0	33.0	40.0	10.0	41.0
50	33.071820889335456	38.0	33.0	40.0	10.0	41.0
51	31.819096066071612	36.0	30.0	40.0	9.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	0.0
9	5.0
10	4.0
11	10.0
12	10.0
13	12.0
14	12.0
15	26.0
16	64.0
17	117.0
18	262.0
19	477.0
20	796.0
21	1211.0
22	1894.0
23	2922.0
24	4535.0
25	8288.0
26	12905.0
27	15506.0
28	15079.0
29	12364.0
30	10729.0
31	10972.0
32	12506.0
33	16654.0
34	25688.0
35	31264.0
36	37498.0
37	55752.0
38	97884.0
39	68431.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.7349250476478	12.999400741638546	16.334746621849952	29.93092758886371
2	40.209334913332825	28.60332250462961	18.331899018207544	12.855443563830027
3	23.00836708855056	27.29914707061787	37.49712761499678	12.195358225834795
4	21.100652876214852	18.000729923718467	46.680649361767315	14.217967838299368
5	25.502273126641768	21.261281151296966	37.07133877922511	16.16510694283616
6	18.18749127020244	32.17544302314579	38.80152833410681	10.835537372544955
7	56.57449502345217	5.341734965598965	33.92883694315156	4.154933067797297
8	54.87224082075867	11.46948963913833	28.309325451358696	5.348944088744306
9	49.68978241965207	7.546825507679969	29.294046615992535	13.469345456675422
10	28.77161047305365	20.52527473517737	36.83749284719813	13.865621944570854
11	22.708287337625766	21.057848707539392	41.008195871875856	15.225668082958984
12	19.801884284562114	18.86875340743711	44.47668524517777	16.852677062823002
13	17.477167355288117	20.734789876588824	48.17924583560496	13.608796932518102
14	14.95419953951726	25.203770371405014	42.42568971032842	17.416340378749307
15	13.235499524648443	21.411546311857656	50.788497844021606	14.56445631947229
16	14.59306752695536	22.723832009407904	42.313497731379066	20.369602732257672
17	14.851920104892741	21.690449263543012	45.20548253815203	18.252148093412213
18	14.826462888785757	21.978588904258338	45.73850708071064	17.456441126245263
19	15.071122505530749	24.02710630302648	42.00778585299697	18.893985338445805
20	16.01619349286522	23.14871970478641	46.608783415412205	14.226303386936168
21	15.365119558801663	26.691778445622937	42.946548857579266	14.996553137996136
22	14.676423013323362	20.833915319837253	43.11348511541356	21.37617655142583
23	14.538097962972142	25.538994597663343	43.7704164620327	16.152490977331812
24	15.985554719497525	21.875408329240653	42.62439116702187	19.514645784239956
25	13.567569759530688	28.680370008245436	41.136608377902235	16.615451854321645
26	15.072023645923915	22.71099075880527	43.01728837844292	19.199697216827897
27	16.649244619065428	23.872335440499953	40.447911832423934	19.03050810801069
28	13.213196299917545	22.624931851257767	44.924777305680344	19.237094543144348
29	19.082323680617822	20.259213034094646	40.54455913959115	20.113904145696377
30	14.454967761702434	24.295871425288702	42.269341852113854	18.979818960895013
31	17.105672228204792	22.38320094079057	38.61364056213138	21.89748626887326
32	17.85069004825607	23.526973384818486	39.01487332218923	19.607463244736216
33	14.394366070261915	23.111547663568246	39.723169671218926	22.77091659495091
34	19.177844562293583	25.109601200319005	36.857768506044394	18.854785731343014
35	15.55796360293952	23.422891669407637	38.32257221513826	22.69657251251459
36	17.650862166071164	25.090677252062488	37.153342555003356	20.105118026862996
37	15.181737488792066	25.77959908263908	36.785677274590995	22.252986153977858
38	15.229047359433363	24.950099350728348	37.38042993408158	22.44042335575671
39	17.803380177614773	26.962571133769785	36.04088473963801	19.193163948977432
40	16.683938524202375	24.78361366309064	41.104392608846496	17.428055203860485
41	15.507950311118721	28.222365403418024	36.74242253571896	19.5272617497443
42	16.39895287486314	23.907029345636904	40.68829103230138	19.00572674719858
43	18.63806146678622	23.350124582659358	37.31261911949572	20.699194831058705
44	17.77949995719583	24.489391324721435	35.78676314876476	21.94434556931797
45	16.002225816771123	23.97033445825692	37.01817149602822	23.00926822894373
46	20.21528243992773	26.194123663496153	35.47451800253221	18.116075894043913
47	14.574819433993719	23.920321166436125	42.51738074533321	18.987478654236938
48	17.716870699870686	24.860435881608176	36.255356153211885	21.16733726530925
49	16.644063061804715	22.165800820938898	41.213205311321474	19.976930805934913
50	16.912602898968647	22.5463073519539	38.72020041362344	21.820889335454016
51	17.454188275262343	21.9574121050189	35.60135351287054	24.987046106848215
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	507.0
1	1019.5
2	1532.0
3	15290.5
4	29049.0
5	19222.0
6	9395.0
7	9000.5
8	8606.0
9	8325.0
10	8044.0
11	7651.0
12	7258.0
13	6755.5
14	6253.0
15	5766.5
16	5280.0
17	4971.0
18	4662.0
19	4339.0
20	4016.0
21	3864.0
22	3712.0
23	3794.0
24	3876.0
25	4230.0
26	5052.5
27	5521.0
28	6256.5
29	6992.0
30	8041.0
31	9090.0
32	10001.5
33	10913.0
34	11795.0
35	12677.0
36	13906.0
37	15135.0
38	16250.0
39	17365.0
40	18803.5
41	20242.0
42	21727.0
43	23212.0
44	24656.5
45	26101.0
46	47332.5
47	68564.0
48	51423.0
49	34282.0
50	32600.0
51	30918.0
52	26157.5
53	21397.0
54	18009.5
55	14622.0
56	12318.5
57	10015.0
58	8216.0
59	6417.0
60	5441.0
61	4465.0
62	3796.5
63	3128.0
64	2565.0
65	2002.0
66	1639.5
67	1277.0
68	1101.5
69	926.0
70	796.5
71	667.0
72	549.5
73	432.0
74	368.5
75	261.5
76	218.0
77	161.0
78	104.0
79	80.0
80	56.0
81	44.0
82	32.0
83	22.5
84	13.0
85	11.5
86	10.0
87	7.0
88	4.0
89	3.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	443882.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.43800575333061
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.25729598695261	32.050069911138166
2	8.759419013721178	7.08426872945381
3	3.1395438610681774	3.8087067815012623
4	1.6984550425742002	2.7472853913355553
5	1.0901967721660109	2.20426916725558
6	0.7910340246162152	1.9192703063106467
7	0.6044878847626934	1.7110999193296714
8	0.48546392153052886	1.5704954281508772
9	0.43397223881586094	1.5794074701019394
>10	3.6266837779325773	26.913143830319175
>50	0.07168074345378285	1.9388842957487635
>100	0.03273608756157011	2.4056316041426675
>500	0.003386491816714149	0.9010847792367412
>1k	0.003386491816714149	2.939252732272227
>5k	0.0011288306055713831	3.819111350295944
>10k+	0.0011288306055713831	6.408018303406977
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17106	3.8537268913810427	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCG	10970	2.471377528262016	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC	9196	2.0717217638922056	RNA PCR Primer, Index 27 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGC	7537	1.697973785825963	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCT	2667	0.6008353571444663	No Hit
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	2411	0.5431623719817429	Illumina PCR Primer Index 6 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTC	2251	0.5071167562550408	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTA	2235	0.5035121946823705	No Hit
GAACTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCT	2083	0.4692688597420035	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCC	1231	0.27732595599731463	No Hit
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	756	0.17031553430866764	RNA PCR Primer, Index 27 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	721	0.16243055586845154	RNA PCR Primer, Index 27 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT	710	0.15995241978724073	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCCTATTT	696	0.15679842841115432	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGT	536	0.12075281268445216	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTAT	529	0.11917581699640895	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATG	474	0.1067851365903551	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGC	469	0.10565871109889566	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.252850982918884E-4	0.0	0.0	0.18946476766347814	0.0
2	2.252850982918884E-4	0.0	0.0	1.0748352039505995	0.0
3	2.252850982918884E-4	0.0	0.0	1.3228740971699686	0.0
4	2.252850982918884E-4	0.0	0.0	2.100558256473567	0.0
5	2.252850982918884E-4	0.0	0.0	4.985784510297782	0.0
6	2.252850982918884E-4	0.0	0.0	5.624918334151869	0.0
7	2.252850982918884E-4	0.0	0.0	6.733771587944544	0.0
8	2.252850982918884E-4	0.0	0.0	7.9568443865712055	0.0
9	2.252850982918884E-4	0.0	0.0	8.253770146119916	0.0
10	2.252850982918884E-4	0.0	0.0	11.555999116882415	0.0
11	2.252850982918884E-4	0.0	0.0	12.693688863256451	0.0
12	2.252850982918884E-4	0.0	0.0	15.450953181250872	0.0
13	2.252850982918884E-4	0.0	0.0	15.941173555134021	0.0
14	2.252850982918884E-4	0.0	0.0	16.213993809165498	0.0
15	2.252850982918884E-4	0.0	0.0	17.039438409306978	0.0
16	2.252850982918884E-4	0.0	0.0	17.68285265002861	0.0
17	2.252850982918884E-4	0.0	0.0	18.312299214656147	0.0
18	2.252850982918884E-4	0.0	0.0	18.973961548339425	0.0
19	2.252850982918884E-4	0.0	0.0	19.536273153675978	0.0
20	2.252850982918884E-4	0.0	0.0	19.97422738475541	0.0
21	2.252850982918884E-4	0.0	0.0	20.450930652741043	0.0
22	2.252850982918884E-4	0.0	0.0	20.997697586295455	0.0
23	4.505701965837768E-4	0.0	0.0	21.499858070388076	0.0
24	4.505701965837768E-4	0.0	0.0	21.902217255937387	0.0
25	4.505701965837768E-4	0.0	0.0	22.25546429005907	0.0
26	4.505701965837768E-4	0.0	0.0	22.609612464573917	0.0
27	4.505701965837768E-4	0.0	0.0	23.0378794364268	0.0
28	4.505701965837768E-4	0.0	0.0	23.406220572134036	0.0
29	4.505701965837768E-4	0.0	0.0	23.809706183174807	0.0
30	4.505701965837768E-4	0.0	0.0	24.26207866054492	0.0
31	4.505701965837768E-4	0.0	0.0	24.65948157393181	0.0
32	6.758552948756652E-4	0.0	0.0	25.05057650456653	0.0
33	6.758552948756652E-4	0.0	0.0	25.425901478320814	0.0
34	6.758552948756652E-4	0.0	0.0	25.78793463127588	0.0
35	6.758552948756652E-4	0.0	0.0	26.17249629406013	0.0
36	6.758552948756652E-4	0.0	0.0	26.574630194511155	0.0
37	6.758552948756652E-4	0.0	0.0	26.961219423180033	0.0
38	6.758552948756652E-4	0.0	0.0	27.346907511455747	0.0
39	6.758552948756652E-4	0.0	0.0	27.74926669700506	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAAGG	25	3.8869224E-5	45.000004	2
TAGGACG	25	3.8869224E-5	45.000004	1
TATACGG	25	3.8869224E-5	45.000004	2
CGATCCA	25	3.8869224E-5	45.000004	27
GCGTAAG	25	3.8869224E-5	45.000004	15
TCACGAC	20	7.0286024E-4	45.0	25
TAGCGAG	20	7.0286024E-4	45.0	1
GCGTTCT	20	7.0286024E-4	45.0	26
GTGTACG	20	7.0286024E-4	45.0	1
GTCAAGC	40	6.7993824E-9	45.0	16
CCGACAC	20	7.0286024E-4	45.0	45
GTATACG	20	7.0286024E-4	45.0	1
TCGACAG	20	7.0286024E-4	45.0	1
CCGTTGT	20	7.0286024E-4	45.0	16
TCGCGTT	20	7.0286024E-4	45.0	32
GCCCGTT	20	7.0286024E-4	45.0	14
CACGTGA	30	2.16248E-6	44.999996	43
CGTTTTT	8480	0.0	43.540684	1
AACGGGA	90	0.0	42.5	4
TGCGAGG	70	0.0	41.785713	2
>>END_MODULE