FastQCFastQC Report
Sat 18 Jun 2016
SRR3552146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306127
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176025.749901184802386No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC106873.4910347666164694RNA PCR Primer, Index 11 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCG97413.182012694077948No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGC90402.9530227650615597No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCT31941.0433578220803783TruSeq Adapter, Index 11 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC29990.9796587690729666Illumina PCR Primer Index 11 (100% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTC28560.9329461302008643No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTACATCTCGTA28140.9192263341684986No Hit
GAACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCT25040.8179611729772284TruSeq Adapter, Index 11 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCC17490.5713315062049411No Hit
CTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGCT11420.37304778735622796TruSeq Adapter, Index 11 (96% over 27bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTACATCT8570.2799491714223182No Hit
CCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC8470.27668255331937397RNA PCR Primer, Index 11 (96% over 26bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTACATCTCGT7670.25054960849582036No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTAT7130.23290987073992167No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGC6910.2257233109134444No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATG6460.2110235294501955No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTT5840.19077049721194145TruSeq Adapter, Index 11 (95% over 22bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCG3540.11563828084422478No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3350.1094317064486308No Hit
TCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC3190.10420511748392006Illumina PCR Primer Index 11 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAC351.2080272E-745.0000045
GCACCGA351.2080272E-745.0000049
AAGGGCG351.2080272E-745.0000045
TATAAGG351.2080272E-745.0000042
GGGTCCA207.02517E-445.07
TCGGGCT253.8840793E-545.05
CGCGCCT207.02517E-445.017
GCGGGTT207.02517E-445.05
CGGGCTA253.8840793E-545.06
TGCCTCA207.02517E-445.019
GGGTTCA207.02517E-445.07
TGCACGG253.8840793E-545.02
GGTAGTG207.02517E-445.013
TGCGTGG207.02517E-445.02
GTGCCTC207.02517E-445.018
CCGTCTC302.1602664E-644.99999619
CGTTTTT75600.044.0773771
TACGGCT11200.043.5937547
GGCGACT3100.043.54838610
ACGGCTG11350.043.017628
Produced by FastQC (version 0.11.5)