##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552146_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	306127
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.583698922342688	31.0	30.0	33.0	28.0	34.0
2	30.515204473960154	31.0	30.0	33.0	26.0	34.0
3	30.15795078513166	31.0	30.0	34.0	25.0	34.0
4	34.41860731003799	35.0	35.0	37.0	32.0	37.0
5	35.08048620343844	35.0	35.0	37.0	33.0	37.0
6	35.02712599672685	35.0	35.0	37.0	32.0	37.0
7	35.50211840183976	37.0	35.0	37.0	33.0	37.0
8	34.841885883963194	37.0	35.0	37.0	32.0	37.0
9	36.784726600397875	39.0	35.0	39.0	32.0	39.0
10	36.63723552643184	38.0	35.0	39.0	32.0	39.0
11	36.92098050808978	39.0	37.0	39.0	33.0	39.0
12	37.102454210180746	39.0	37.0	39.0	34.0	39.0
13	36.964926321428685	39.0	37.0	39.0	33.0	39.0
14	38.12860675471291	40.0	37.0	41.0	33.0	41.0
15	38.12901834859388	40.0	37.0	41.0	33.0	41.0
16	38.016088094157	40.0	37.0	41.0	33.0	41.0
17	37.689298885756564	39.0	36.0	41.0	32.0	41.0
18	37.34753549997223	39.0	36.0	40.0	33.0	41.0
19	36.65296755921562	37.0	36.0	40.0	32.0	41.0
20	36.15897650321599	37.0	35.0	40.0	32.0	41.0
21	36.49844345647395	37.0	35.0	40.0	32.0	41.0
22	36.84628928516596	38.0	35.0	40.0	33.0	41.0
23	36.9001427512111	38.0	35.0	40.0	33.0	41.0
24	36.79086457581331	38.0	35.0	40.0	33.0	41.0
25	36.33871889771239	37.0	35.0	40.0	32.0	41.0
26	36.5087104371715	38.0	35.0	40.0	33.0	41.0
27	36.724395430654596	38.0	35.0	40.0	33.0	41.0
28	36.49309926925753	38.0	35.0	40.0	33.0	41.0
29	36.477311703966	38.0	35.0	40.0	32.0	41.0
30	36.08117219324006	38.0	35.0	40.0	31.0	41.0
31	35.7237355737978	38.0	35.0	40.0	30.0	41.0
32	35.30022833660539	38.0	35.0	40.0	27.0	41.0
33	34.56484073603439	38.0	35.0	40.0	21.0	41.0
34	33.95385575267781	38.0	34.0	40.0	15.0	41.0
35	33.726544865366336	38.0	34.0	40.0	13.0	41.0
36	33.2648410626962	38.0	33.0	40.0	10.0	41.0
37	32.95600518738955	38.0	33.0	40.0	10.0	41.0
38	32.80471830318789	38.0	32.0	40.0	10.0	41.0
39	32.8111666073231	38.0	32.0	40.0	10.0	41.0
40	32.82297869838335	38.0	33.0	40.0	10.0	41.0
41	32.49563416490542	37.0	31.0	40.0	10.0	41.0
42	32.476527715621295	37.0	31.0	40.0	10.0	41.0
43	32.551421468867495	38.0	31.0	40.0	10.0	41.0
44	32.56011393963943	38.0	32.0	40.0	10.0	41.0
45	32.482502360131576	37.0	31.0	40.0	10.0	41.0
46	32.33200273089273	37.0	31.0	40.0	10.0	41.0
47	32.2409849506904	37.0	31.0	40.0	10.0	41.0
48	32.27314807253199	37.0	31.0	40.0	10.0	41.0
49	32.259604673877185	37.0	31.0	40.0	8.0	41.0
50	32.11091801768547	37.0	31.0	40.0	8.0	41.0
51	30.718038591826268	35.0	27.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	4.0
11	7.0
12	5.0
13	8.0
14	6.0
15	18.0
16	40.0
17	89.0
18	205.0
19	370.0
20	574.0
21	934.0
22	1404.0
23	2252.0
24	3427.0
25	5917.0
26	9914.0
27	11992.0
28	11680.0
29	9848.0
30	8877.0
31	9016.0
32	9949.0
33	12512.0
34	18689.0
35	25232.0
36	29777.0
37	40166.0
38	60229.0
39	32979.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.965765842281144	11.857170390066868	14.533510601809052	33.64355316584293
2	42.80837691546319	31.136097110022966	14.668421929460647	11.387104045053196
3	19.190728031176604	29.979387639770422	40.75661408500393	10.07327024404904
4	18.236548883306604	15.04179637862717	55.00364227918478	11.718012458881445
5	26.489659520395133	17.47085359997648	40.468171706513964	15.571315173114424
6	15.400471046330445	31.99913761282082	42.57024045575856	10.030150885090174
7	47.47114759560574	4.675837152554331	43.09289935222963	4.760115899610293
8	43.719436704374324	14.046784504470367	35.58065770088884	6.653121090266458
9	40.29765424154028	6.213760955420463	35.46207946375197	18.026505339287286
10	27.867519036216994	16.313817467913644	42.27134489933916	13.547318596530197
11	22.505038758423794	16.267104829041543	45.06234340649469	16.165513006039976
12	18.50081828783479	14.048417813521837	48.85390703858203	18.596856860061347
13	16.299771010070984	17.463667040150003	53.48433819950543	12.752223750273577
14	14.262707961074977	23.682981246345474	44.43384608348823	17.620464709091323
15	10.569142872075968	17.865787728622433	56.82053526804235	14.744534131259249
16	11.13982105466032	20.92628222927086	45.817585511895395	22.11631120417343
17	11.908782956093386	20.084148082331843	49.31874679463098	18.688322166943784
18	11.979015245306686	20.05801513750829	49.65945506276807	18.303514554416957
19	11.926422693849286	21.82656217844228	46.01619589255439	20.230819235154037
20	13.638130579792046	20.973321529953253	52.15351798436597	13.235029905888732
21	13.13866467185188	25.811509602223914	46.31051818362967	14.739307542294538
22	11.535408506926863	18.982971119829354	47.083073364975974	22.398547008267812
23	12.322336807926122	24.762271867558237	47.591032479983795	15.324358844531844
24	16.028641707526614	18.211722585724225	44.47239217710297	21.287243529646194
25	11.08363522328968	28.342811970195374	43.13046546041349	17.443087346101454
26	13.98341211327325	19.037196980338226	46.285038562426706	20.694352343961818
27	17.311769298363096	22.152897326926407	42.37979662035691	18.155536754353584
28	11.367504336435532	20.201746334037836	49.37362597876045	19.057123350766187
29	20.277205212215847	17.61719809098838	42.6437393630748	19.46185733372097
30	17.97064616972694	21.797489277326076	44.825513594031236	15.406350958915743
31	17.10956563779085	21.108559519415145	43.67272406550223	18.109150777291777
32	18.180036390125668	26.29006915430524	40.045471323992984	15.484423131576111
33	14.243761576077901	20.444129397276296	38.47488134009741	26.837227686548392
34	19.862671374952225	24.977215338731966	38.63821224524462	16.52190104107119
35	18.012132219634335	22.147017414341107	38.3585897356326	21.482260630391963
36	19.44029765424154	22.376987328788378	37.41453710388172	20.76817791308836
37	18.067664727384386	26.731389260013	40.126810114756296	15.074135897846318
38	14.647188911791512	23.225981373743576	38.190032241520676	23.936797472944235
39	18.412619599055294	28.1716411816011	35.5078121171932	17.907927102150413
40	16.88710894498035	22.01994597013658	41.09862900038219	19.994316084500877
41	15.085895723016918	27.97368412456268	34.92406746219706	22.01635269022334
42	16.692418506044877	21.28463023516384	43.9611664439922	18.061784814799086
43	19.540582830001927	21.376422203856567	34.838482067899925	24.24451289824158
44	17.516259591607405	22.476945842738473	37.50306245447151	22.503732111182615
45	16.534967513482965	20.418976437883625	38.27365766495605	24.772398383677363
46	22.053265474786606	24.003436482244297	35.786454641374334	18.156843401594763
47	13.348708215871191	20.208606232054017	46.94652872827291	19.496156823801886
48	17.015160374615764	22.808507580187307	37.46647633171853	22.709855713478394
49	15.885563834617658	18.871252780708662	45.34457921058907	19.89860417408461
50	17.221283976911543	18.564190679031906	40.65012233484796	23.564403009208597
51	15.961349374605966	18.882032620448378	36.5256903180706	28.630927686875058
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	334.0
1	697.5
2	1061.0
3	14910.5
4	28760.0
5	18162.0
6	7564.0
7	7090.5
8	6617.0
9	6378.5
10	6140.0
11	5865.0
12	5590.0
13	5176.0
14	4762.0
15	4457.5
16	4153.0
17	3776.0
18	3399.0
19	3165.5
20	2932.0
21	2712.0
22	2492.0
23	2422.5
24	2353.0
25	2414.0
26	2770.5
27	3066.0
28	3438.0
29	3810.0
30	4082.0
31	4354.0
32	4914.5
33	5475.0
34	5829.5
35	6184.0
36	6708.0
37	7232.0
38	7484.5
39	7737.0
40	8650.5
41	9564.0
42	11186.5
43	12809.0
44	13730.5
45	14652.0
46	37575.5
47	60499.0
48	42824.5
49	25150.0
50	24208.5
51	23267.0
52	18859.0
53	14451.0
54	11973.5
55	9496.0
56	7971.0
57	6446.0
58	5386.0
59	4326.0
60	3727.0
61	3128.0
62	2599.5
63	2071.0
64	1695.5
65	1320.0
66	1071.0
67	822.0
68	701.0
69	580.0
70	466.5
71	353.0
72	327.0
73	301.0
74	228.0
75	123.0
76	91.0
77	82.0
78	73.0
79	59.5
80	46.0
81	35.0
82	24.0
83	15.5
84	7.0
85	5.5
86	4.0
87	2.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	306127.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.18831648429446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.43416129311737	35.98438491853352
2	8.567723763389228	7.571865784133074
3	3.003387943902659	3.9814397097085537
4	1.6553060063691523	2.925807427511746
5	1.0641503726660948	2.3511506727124645
6	0.7408747243000916	1.964280407555211
7	0.6066465196519555	1.876468188313346
8	0.4980206965608349	1.7605356924287163
9	0.40508568254087973	1.6110048909035544
>10	1.9250461750445487	13.260209461086896
>50	0.054257970443067065	1.6290327553259396
>100	0.031960174370573746	2.279660555386562
>500	0.005202819083581773	1.6766556886973631
>1k	0.005202819083581773	5.668114373269167
>5k	0.0014865197381662208	6.1683193906807405
>10k+	0.0014865197381662208	9.291070083753127
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17602	5.749901184802386	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTC	10687	3.4910347666164694	RNA PCR Primer, Index 11 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCG	9741	3.182012694077948	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGC	9040	2.9530227650615597	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCT	3194	1.0433578220803783	TruSeq Adapter, Index 11 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	2999	0.9796587690729666	Illumina PCR Primer Index 11 (100% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTC	2856	0.9329461302008643	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTACATCTCGTA	2814	0.9192263341684986	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCT	2504	0.8179611729772284	TruSeq Adapter, Index 11 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCC	1749	0.5713315062049411	No Hit
CTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGCT	1142	0.37304778735622796	TruSeq Adapter, Index 11 (96% over 27bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTACATCT	857	0.2799491714223182	No Hit
CCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	847	0.27668255331937397	RNA PCR Primer, Index 11 (96% over 26bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTACATCTCGT	767	0.25054960849582036	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTAT	713	0.23290987073992167	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGC	691	0.2257233109134444	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATG	646	0.2110235294501955	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTT	584	0.19077049721194145	TruSeq Adapter, Index 11 (95% over 22bp)
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCG	354	0.11563828084422478	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	335	0.1094317064486308	No Hit
TCTGTCTCTTATACACATCTGACGCGGTACATCTCGTATGCCGTCTTCTGC	319	0.10420511748392006	Illumina PCR Primer Index 11 (100% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.39885407036948717	0.0
2	0.0	0.0	0.0	1.9315512842709073	0.0
3	0.0	0.0	0.0	2.323218794813917	0.0
4	0.0	0.0	0.0	3.5795601172062574	0.0
5	0.0	0.0	0.0	8.14008565072666	0.0
6	0.0	0.0	0.0	8.972419943356842	0.0
7	0.0	0.0	0.0	10.707320817830508	0.0
8	0.0	0.0	0.0	12.646385323738187	0.0
9	0.0	0.0	0.0	13.112205065218031	0.0
10	0.0	0.0	0.0	17.32842905068811	0.0
11	0.0	0.0	0.0	19.138462141529494	0.0
12	0.0	0.0	0.0	23.603602426443928	0.0
13	0.0	0.0	0.0	24.335978205124018	0.0
14	0.0	0.0	0.0	24.796244695828854	0.0
15	0.0	0.0	0.0	26.127391572778617	0.0
16	0.0	0.0	0.0	27.06687093918537	0.0
17	0.0	0.0	0.0	27.861312461821402	0.0
18	0.0	0.0	0.0	28.680580282039806	0.0
19	0.0	0.0	0.0	29.5593005517318	0.0
20	0.0	0.0	0.0	30.126058792592616	0.0
21	0.0	0.0	0.0	30.73822302508436	0.0
22	0.0	0.0	0.0	31.421926194030583	0.0
23	0.0	0.0	0.0	32.038990353676745	0.0
24	0.0	0.0	0.0	32.475737194040384	0.0
25	0.0	0.0	0.0	32.905624136387836	0.0
26	0.0	0.0	0.0	33.30513153037791	0.0
27	0.0	0.0	0.0	33.73501847272537	0.0
28	0.0	0.0	0.0	34.1315859104228	0.0
29	0.0	0.0	0.0	34.51051361036433	0.0
30	0.0	0.0	0.0	34.98580654434271	0.0
31	0.0	0.0	0.0	35.40491364695045	0.0
32	0.0	0.0	0.0	35.789067935856686	0.0
33	0.0	0.0	0.0	36.16113573778203	0.0
34	0.0	0.0	0.0	36.53581683418973	0.0
35	0.0	0.0	0.0	36.94545074429894	0.0
36	0.0	0.0	0.0	37.3220918115684	0.0
37	0.0	0.0	0.0	37.70101951150993	0.0
38	0.0	0.0	0.0	38.08092719688234	0.0
39	0.0	0.0	0.0	38.47357469285623	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGAC	35	1.2080272E-7	45.000004	5
GCACCGA	35	1.2080272E-7	45.000004	9
AAGGGCG	35	1.2080272E-7	45.000004	5
TATAAGG	35	1.2080272E-7	45.000004	2
GGGTCCA	20	7.02517E-4	45.0	7
TCGGGCT	25	3.8840793E-5	45.0	5
CGCGCCT	20	7.02517E-4	45.0	17
GCGGGTT	20	7.02517E-4	45.0	5
CGGGCTA	25	3.8840793E-5	45.0	6
TGCCTCA	20	7.02517E-4	45.0	19
GGGTTCA	20	7.02517E-4	45.0	7
TGCACGG	25	3.8840793E-5	45.0	2
GGTAGTG	20	7.02517E-4	45.0	13
TGCGTGG	20	7.02517E-4	45.0	2
GTGCCTC	20	7.02517E-4	45.0	18
CCGTCTC	30	2.1602664E-6	44.999996	19
CGTTTTT	7560	0.0	44.077377	1
TACGGCT	1120	0.0	43.593754	7
GGCGACT	310	0.0	43.548386	10
ACGGCTG	1135	0.0	43.01762	8
>>END_MODULE