SRR3552145_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552145_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	483522
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15034	3.1092690715210476	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCG	10178	2.104971438734949	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTC	9368	1.9374506227224408	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGC	7767	1.6063384913199399	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	2715	0.561504957375259	TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTA	2458	0.5083532910601792	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCT	2418	0.5000806581706727	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTTGCCT	2271	0.469678732301736	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT	2067	0.42748830456525244	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTC	1891	0.39108871985142357	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCC	1855	0.3836433502508676	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	908	0.18778876659179936	TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGCT	867	0.17930931788005508	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGC	724	0.14973465530006907	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGT	685	0.14166883823280016	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	572	0.11829865031994406	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTAT	542	0.11209417565281414	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATG	521	0.1077510433858232	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTT	509	0.10526925351897122	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTTGTTGCC	503	0.10402835858554521	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGACGG	45	3.8380676E-10	45.000004	2
ACGGGCC	45	3.8380676E-10	45.000004	5
TTTAGCG	25	3.8874412E-5	45.0	1
GTCGAAA	20	7.0292293E-4	45.0	8
GACGTAG	20	7.0292293E-4	45.0	1
ATAGCGG	40	6.8012014E-9	45.0	2
ATTCGTA	20	7.0292293E-4	45.0	32
GTTAGCG	25	3.8874412E-5	45.0	1
TTCGTAC	20	7.0292293E-4	45.0	33
GCGTTGA	20	7.0292293E-4	45.0	17
TCGGCGT	25	3.8874412E-5	45.0	4
ACGATAT	20	7.0292293E-4	45.0	32
TACGAGG	20	7.0292293E-4	45.0	2
TCGCTGG	20	7.0292293E-4	45.0	2
CGGGCCC	20	7.0292293E-4	45.0	6
AATTCGT	20	7.0292293E-4	45.0	31
TCGTACA	20	7.0292293E-4	45.0	34
CCGACAG	20	7.0292293E-4	45.0	1
CGCACTA	20	7.0292293E-4	45.0	39
ATACGAG	20	7.0292293E-4	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content