##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552145_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	483522
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.839086949507987	30.0	28.0	33.0	25.0	33.0
2	28.409832437820825	31.0	27.0	33.0	16.0	34.0
3	28.183867538602172	31.0	25.0	33.0	16.0	34.0
4	33.34705349498058	35.0	32.0	35.0	28.0	37.0
5	34.33777780535322	35.0	33.0	35.0	32.0	37.0
6	33.479670004674034	35.0	33.0	37.0	28.0	37.0
7	34.61570311175086	35.0	35.0	37.0	32.0	37.0
8	33.94280094804373	35.0	35.0	37.0	28.0	37.0
9	36.23896534180451	39.0	35.0	39.0	31.0	39.0
10	35.95476937967662	37.0	35.0	39.0	31.0	39.0
11	36.49441390464136	37.0	35.0	39.0	32.0	39.0
12	36.55873982983194	39.0	35.0	39.0	32.0	39.0
13	36.567291664081466	38.0	35.0	39.0	32.0	39.0
14	37.38034256972796	39.0	36.0	40.0	32.0	41.0
15	37.6546175768631	39.0	37.0	41.0	32.0	41.0
16	37.18438664631599	39.0	36.0	41.0	32.0	41.0
17	37.0775538651809	38.0	36.0	40.0	32.0	41.0
18	36.83471486302588	39.0	36.0	40.0	32.0	41.0
19	36.15032614855167	37.0	34.0	40.0	30.0	41.0
20	36.07576490831855	37.0	35.0	40.0	31.0	41.0
21	36.441760664457874	38.0	35.0	40.0	32.0	41.0
22	36.90040370448501	38.0	35.0	40.0	33.0	41.0
23	37.10507484664607	39.0	35.0	40.0	33.0	41.0
24	37.06301057656115	39.0	35.0	40.0	33.0	41.0
25	36.34985998568835	38.0	35.0	40.0	31.0	41.0
26	36.66210224146988	38.0	35.0	40.0	32.0	41.0
27	36.84981448620745	38.0	35.0	40.0	33.0	41.0
28	36.726430234818686	38.0	35.0	40.0	32.0	41.0
29	36.801109360070484	39.0	35.0	40.0	32.0	41.0
30	36.23939965503121	38.0	35.0	40.0	31.0	41.0
31	36.20866268753025	38.0	35.0	40.0	31.0	41.0
32	35.71233780469141	38.0	35.0	40.0	29.0	41.0
33	35.344681317499514	39.0	35.0	40.0	23.0	41.0
34	34.89133896699633	39.0	35.0	40.0	20.0	41.0
35	34.590217197976514	39.0	35.0	40.0	17.0	41.0
36	34.36258536323063	39.0	35.0	40.0	15.0	41.0
37	34.32000405359012	39.0	35.0	40.0	15.0	41.0
38	34.1764387142674	39.0	34.0	40.0	15.0	41.0
39	34.02960775311154	39.0	34.0	40.0	12.0	41.0
40	33.94496630970256	38.0	34.0	40.0	13.0	41.0
41	33.77467002535562	38.0	33.0	40.0	12.0	41.0
42	33.89483622255037	39.0	34.0	40.0	12.0	41.0
43	33.91918051298597	39.0	34.0	40.0	11.0	41.0
44	33.962845537535	39.0	34.0	40.0	12.0	41.0
45	33.96769330040826	39.0	34.0	40.0	12.0	41.0
46	33.78786487481438	38.0	34.0	40.0	10.0	41.0
47	33.66497904955721	38.0	33.0	40.0	10.0	41.0
48	33.64360256617072	38.0	34.0	40.0	10.0	41.0
49	33.69540372516659	38.0	34.0	40.0	10.0	41.0
50	33.6690822754704	38.0	34.0	40.0	10.0	41.0
51	32.775422834948564	37.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	12.0
11	7.0
12	17.0
13	15.0
14	16.0
15	27.0
16	48.0
17	120.0
18	299.0
19	659.0
20	1284.0
21	1984.0
22	2832.0
23	3641.0
24	5394.0
25	8645.0
26	13001.0
27	14958.0
28	13974.0
29	12202.0
30	11495.0
31	13185.0
32	16167.0
33	22172.0
34	30076.0
35	38743.0
36	53396.0
37	83840.0
38	104045.0
39	31259.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.989034625104956	13.663907743598017	17.651730427984663	27.695327203312363
2	39.08426090229607	28.335835804782413	19.292400345796054	13.287502947125468
3	25.002792013600207	26.82442577586956	35.509863046562515	12.66291916396772
4	21.83850993336394	19.226219282680002	44.90074908690814	14.03452169704791
5	26.369430966946695	22.15059500912058	35.169030571514845	16.310943452417884
6	18.6436191114365	33.21668920959129	36.21345047381505	11.92624120515716
7	59.42025388710338	5.13068691807198	31.51025186030832	3.93880733451632
8	58.27738965341804	11.443946707698926	25.011685093956427	5.266978544926601
9	53.060046905828486	7.319625580635421	25.863766281575607	13.75656123196049
10	30.323129040664128	22.580565103552683	33.286386141685384	13.809919714097807
11	24.085563841976164	21.149399613668045	39.13720575278891	15.627830791566877
12	19.870450568950325	19.51079785407903	42.00429349646965	18.614458080500988
13	19.56043365141607	21.266043737410087	45.750968932127186	13.422553679046661
14	15.915097968654992	26.658973118079427	39.71629005505437	17.709638858211207
15	14.057685069138529	21.80066263789445	47.54116668941641	16.600485603550613
16	15.37613593590364	23.498413722643438	39.98639151889677	21.139058822556162
17	15.806726477802457	23.662005038033428	42.58006874557931	17.9511997385848
18	15.93391820847862	22.02650551577798	43.08966293157292	18.949913344170483
19	16.12005244849252	23.389008152679715	40.56485537369551	19.92608402513226
20	17.920590996893626	23.411344261481382	44.541923635325794	14.126141106299198
21	16.451991843183972	27.486856854496793	41.17454841765215	14.886602884667088
22	15.743027204553256	21.48733666720439	42.113905882255615	20.65573024598674
23	16.32252513846319	25.57008781399812	41.38860279366813	16.71878425387056
24	16.567601887814824	23.247959761913624	40.65316572979099	19.531272620480557
25	13.505280007941728	29.36102183561451	39.69354031460823	17.440157841835532
26	14.531086486240543	22.897613759043022	43.2542883260741	19.317011428642335
27	17.875711963468053	24.212341941007857	39.81990478199544	18.09204131352865
28	13.867621328502116	24.376140072220085	43.838749839717735	17.91748875956006
29	16.518793353766736	20.876816359958802	44.07700166693553	18.527388619338932
30	15.57095644045152	25.95311071678228	43.29771964874401	15.178213194022195
31	20.3386815904964	22.523897568259564	38.34923747006341	18.78818337118063
32	18.37806759568334	24.255359632033286	40.88107676589689	16.485496006386473
33	17.48131419046083	22.300536480242886	39.12272864523227	21.095420684064013
34	20.115734134124196	22.951385872824815	38.52254912909857	18.410330863952414
35	16.09254594413491	23.15634035266234	39.12293546105452	21.628178242148238
36	19.77428121160981	24.902486339814942	35.99650067628774	19.326731772287506
37	17.378733542630943	24.36166296466345	40.931746642345125	17.32785685036048
38	18.826444298294597	25.066904918493883	35.3977688709097	20.70888191230182
39	21.13450887446693	23.848139278047327	37.18900070731011	17.828351140175627
40	18.028135224457213	23.958992558766713	38.06445208284215	19.948420133933926
41	19.20988083272323	24.994312564888464	32.99063951588552	22.805167086502788
42	17.329304561116142	23.509374961222033	37.45992943444145	21.70139104322037
43	19.080000496357975	23.06265278518868	34.993443938435064	22.863902780018282
44	18.96356318843817	23.77451284533072	36.40765053089622	20.854273435334896
45	18.13981576846555	22.986544562605218	35.95038074792874	22.923258921000492
46	20.86750964795811	25.869763940420498	34.75581255868399	18.506913852937405
47	16.025537617729906	23.421478236771026	41.47898130798598	19.07400283751308
48	18.958806424526703	24.51470667311932	35.482356542204904	21.044130360149072
49	18.01241722196715	21.963840321639967	39.53015581504047	20.493586641352408
50	17.97084724169738	22.427314579274572	37.07318384685702	22.528654332171026
51	18.019035328278754	21.72786346846679	34.85756594322492	25.395535260029533
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	303.0
1	846.5
2	1390.0
3	13449.0
4	25508.0
5	16946.5
6	8385.0
7	8126.5
8	7868.0
9	7568.0
10	7268.0
11	7069.0
12	6870.0
13	6484.5
14	6099.0
15	5645.0
16	5191.0
17	4868.0
18	4545.0
19	4423.5
20	4302.0
21	4226.0
22	4150.0
23	4304.0
24	4458.0
25	4638.5
26	5608.0
27	6397.0
28	7646.0
29	8895.0
30	9453.5
31	10012.0
32	11283.5
33	12555.0
34	13815.5
35	15076.0
36	15921.5
37	16767.0
38	17756.5
39	18746.0
40	21243.5
41	23741.0
42	25207.0
43	26673.0
44	28266.0
45	29859.0
46	50506.5
47	71154.0
48	55885.5
49	40617.0
50	38265.5
51	35914.0
52	29916.0
53	23918.0
54	20553.0
55	17188.0
56	14346.5
57	11505.0
58	9403.0
59	7301.0
60	6177.0
61	5053.0
62	4298.5
63	3544.0
64	3025.5
65	2507.0
66	2068.0
67	1629.0
68	1370.5
69	1112.0
70	940.5
71	769.0
72	677.0
73	585.0
74	477.0
75	297.5
76	226.0
77	186.5
78	147.0
79	103.5
80	60.0
81	39.5
82	19.0
83	18.0
84	17.0
85	11.5
86	6.0
87	4.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	483522.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.299173164529364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.32316472289618	31.563587779619507
2	8.75569998891005	7.0569494005950775
3	3.260324937482817	3.941651976847602
4	1.8720269662000546	3.017645555182582
5	1.198715515748337	2.415362207207518
6	0.8562894340587617	2.0704653709254535
7	0.7391167293140877	2.0850055144400295
8	0.5581067938600064	1.799299386405175
9	0.48774316879416574	1.7690081777147122
>10	3.8493754173281243	27.31553444876979
>50	0.06370139596412164	1.7625426211674986
>100	0.025376978880015166	1.8762887109882114
>500	0.004661077753472173	1.216978806871219
>1k	0.003625282697145023	3.271504509982226
>5k	0.0010357950563271495	3.576218805648832
>10k+	0.0010357950563271495	5.26195672763457
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15034	3.1092690715210476	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCG	10178	2.104971438734949	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTC	9368	1.9374506227224408	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGC	7767	1.6063384913199399	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	2715	0.561504957375259	TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTA	2458	0.5083532910601792	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCT	2418	0.5000806581706727	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTTGCCT	2271	0.469678732301736	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCT	2067	0.42748830456525244	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTC	1891	0.39108871985142357	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCC	1855	0.3836433502508676	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	908	0.18778876659179936	TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGCT	867	0.17930931788005508	TruSeq Adapter, Index 14 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGC	724	0.14973465530006907	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGT	685	0.14166883823280016	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	572	0.11829865031994406	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTAT	542	0.11209417565281414	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATG	521	0.1077510433858232	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTT	509	0.10526925351897122	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTTGTTGCC	503	0.10402835858554521	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18530697672494736	0.0
2	0.0	0.0	0.0	1.0686173535020123	0.0
3	0.0	0.0	0.0	1.291357994051977	0.0
4	0.0	0.0	0.0	1.9906022890375206	0.0
5	0.0	0.0	0.0	4.628331285856693	0.0
6	0.0	0.0	0.0	5.225615380479068	0.0
7	0.0	0.0	0.0	6.226603960109364	0.0
8	0.0	0.0	0.0	7.294807681966901	0.0
9	0.0	0.0	0.0	7.624265286791501	0.0
10	0.0	0.0	0.0	10.545952407542986	0.0
11	0.0	0.0	0.0	11.799876737769946	0.0
12	0.0	0.0	0.0	14.445878367478626	0.0
13	0.0	0.0	0.0	14.92631152253672	0.0
14	0.0	0.0	0.0	15.200135671179387	0.0
15	0.0	0.0	0.0	15.975074557103916	0.0
16	0.0	0.0	0.0	16.59903789279495	0.0
17	0.0	0.0	0.0	17.24575096893213	0.0
18	0.0	0.0	0.0	17.891429965958114	0.0
19	0.0	0.0	0.0	18.816103507182714	0.0
20	0.0	0.0	0.0	19.313288743842058	0.0
21	0.0	0.0	0.0	19.78255384449932	0.0
22	0.0	0.0	0.0	20.326065825339903	0.0
23	0.0	0.0	0.0	20.787471924752133	0.0
24	0.0	0.0	0.0	21.1584995098465	0.0
25	0.0	0.0	0.0	21.50119332729431	0.0
26	0.0	0.0	0.0	21.850918882698203	0.0
27	0.0	0.0	0.0	22.25152113037256	0.0
28	0.0	0.0	0.0	22.600626238309736	0.0
29	0.0	0.0	0.0	22.950765425358103	0.0
30	0.0	0.0	0.0	23.37949462485678	0.0
31	0.0	0.0	0.0	23.743076840350593	0.0
32	2.0681582223766446E-4	0.0	0.0	24.124652032379085	0.0
33	2.0681582223766446E-4	0.0	0.0	24.47665256182759	0.0
34	2.0681582223766446E-4	0.0	0.0	24.823482695720152	0.0
35	2.0681582223766446E-4	0.0	0.0	25.195544359925712	0.0
36	2.0681582223766446E-4	0.0	0.0	25.560160654530716	0.0
37	2.0681582223766446E-4	0.0	0.0	25.935324556069837	0.0
38	2.0681582223766446E-4	0.0	0.0	26.311315720897912	0.0
39	2.0681582223766446E-4	0.0	0.0	26.687513701548223	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGACGG	45	3.8380676E-10	45.000004	2
ACGGGCC	45	3.8380676E-10	45.000004	5
TTTAGCG	25	3.8874412E-5	45.0	1
GTCGAAA	20	7.0292293E-4	45.0	8
GACGTAG	20	7.0292293E-4	45.0	1
ATAGCGG	40	6.8012014E-9	45.0	2
ATTCGTA	20	7.0292293E-4	45.0	32
GTTAGCG	25	3.8874412E-5	45.0	1
TTCGTAC	20	7.0292293E-4	45.0	33
GCGTTGA	20	7.0292293E-4	45.0	17
TCGGCGT	25	3.8874412E-5	45.0	4
ACGATAT	20	7.0292293E-4	45.0	32
TACGAGG	20	7.0292293E-4	45.0	2
TCGCTGG	20	7.0292293E-4	45.0	2
CGGGCCC	20	7.0292293E-4	45.0	6
AATTCGT	20	7.0292293E-4	45.0	31
TCGTACA	20	7.0292293E-4	45.0	34
CCGACAG	20	7.0292293E-4	45.0	1
CGCACTA	20	7.0292293E-4	45.0	39
ATACGAG	20	7.0292293E-4	45.0	1
>>END_MODULE