SRR3552141_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552141_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	545095
Sequences flagged as poor quality	0
Sequence length	51
%GC	37

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26516	4.8644731652280795	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCG	9326	1.7108944312459295	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTC	8801	1.614580944605986	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGC	7692	1.4111301699703722	No Hit
GCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	3125	0.573294563332997	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTA	2829	0.5189921022940955	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTACAGGT	2605	0.4778983479943863	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCT	2393	0.43900604481787575	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCT	2259	0.4144231739421569	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTC	1862	0.34159183261633297	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCC	1731	0.3175593245214137	No Hit
CTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT	1145	0.2100551280052101	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	1120	0.20546877149854614	Illumina Single End Adapter 2 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	966	0.17721681541749604	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATG	751	0.13777414946018585	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCTACAGGTCGT	736	0.13502233555618745	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGC	710	0.13025252478925692	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	667	0.12236399159779487	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACGGC	25	3.8880964E-5	45.000004	12
ATCGCAT	25	3.8880964E-5	45.000004	38
TCCGGCA	20	7.030018E-4	45.0	4
GGCGTAG	20	7.030018E-4	45.0	1
ACTTCGC	20	7.030018E-4	45.0	14
GTTAGCG	40	6.8030204E-9	45.0	1
GTAGGTA	20	7.030018E-4	45.0	4
TTGAGCG	35	1.2103555E-7	45.0	1
TTAACGG	20	7.030018E-4	45.0	2
CGTAAGG	40	6.8030204E-9	45.0	2
CCGTTGG	20	7.030018E-4	45.0	1
CGGGTCA	30	2.1633932E-6	44.999996	6
CGTTTTT	11505	0.0	43.35724	1
ATCCGGC	80	0.0	42.1875	6
TATGCGG	65	0.0	41.53846	2
CGGGATC	60	3.6379788E-12	41.249996	6
TGATCCG	95	0.0	40.26316	4
ACTAAGG	45	1.9250365E-8	40.000004	2
AGTACGG	45	1.9250365E-8	40.000004	2
ATGTGCG	40	3.4537698E-7	39.375	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content