##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552141_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	545095
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.950060081270237	30.0	28.0	33.0	25.0	33.0
2	28.568686192315102	31.0	28.0	33.0	16.0	34.0
3	28.411405351360774	31.0	26.0	33.0	16.0	34.0
4	33.41682825929425	35.0	32.0	35.0	28.0	37.0
5	34.42351700162357	35.0	35.0	35.0	32.0	37.0
6	33.573848595199	35.0	33.0	37.0	28.0	37.0
7	34.72947100964052	35.0	35.0	37.0	32.0	37.0
8	34.00850310496335	35.0	35.0	37.0	30.0	37.0
9	36.301037433841806	39.0	35.0	39.0	31.0	39.0
10	36.13159724451701	37.0	35.0	39.0	32.0	39.0
11	36.67065740834167	38.0	35.0	39.0	32.0	39.0
12	36.7133527183335	39.0	35.0	39.0	32.0	39.0
13	36.73271448096203	39.0	35.0	39.0	33.0	39.0
14	37.50623102394995	39.0	36.0	41.0	32.0	41.0
15	37.81002944440877	39.0	37.0	41.0	33.0	41.0
16	37.34053330153459	39.0	36.0	41.0	32.0	41.0
17	37.24848512644585	39.0	36.0	41.0	32.0	41.0
18	36.94571771892973	39.0	36.0	39.0	32.0	41.0
19	36.19969546592796	37.0	35.0	40.0	31.0	41.0
20	36.094623872902886	37.0	35.0	39.0	31.0	41.0
21	36.3770150157312	37.0	35.0	40.0	32.0	41.0
22	36.82502132655775	38.0	35.0	40.0	33.0	41.0
23	37.00311321879672	38.0	35.0	40.0	33.0	41.0
24	36.9335858886983	38.0	35.0	40.0	33.0	41.0
25	36.16699474403544	37.0	35.0	40.0	31.0	41.0
26	36.469343875838156	38.0	35.0	40.0	32.0	41.0
27	36.71453232922701	38.0	35.0	40.0	33.0	41.0
28	36.56742035791926	38.0	35.0	40.0	33.0	41.0
29	36.57485575908787	38.0	35.0	40.0	32.0	41.0
30	36.0459075940891	38.0	35.0	40.0	31.0	41.0
31	35.909278199212984	38.0	35.0	40.0	30.0	41.0
32	35.370916996119945	38.0	35.0	40.0	27.0	41.0
33	34.882653482420494	38.0	35.0	40.0	21.0	41.0
34	34.318605013804934	38.0	34.0	40.0	18.0	41.0
35	34.02664673130372	38.0	34.0	40.0	15.0	41.0
36	33.69749309753346	38.0	34.0	40.0	12.0	41.0
37	33.59729955328888	38.0	34.0	40.0	10.0	41.0
38	33.49426063346756	38.0	34.0	40.0	10.0	41.0
39	33.31890954787698	38.0	33.0	40.0	10.0	41.0
40	33.217086929801226	38.0	33.0	40.0	10.0	41.0
41	33.01064585072327	38.0	33.0	40.0	10.0	41.0
42	33.073877030609346	38.0	33.0	40.0	10.0	41.0
43	33.06735890074207	38.0	33.0	40.0	10.0	41.0
44	33.052042304552415	38.0	33.0	40.0	10.0	41.0
45	33.15551968005577	38.0	33.0	40.0	10.0	41.0
46	33.02766306790559	38.0	33.0	40.0	10.0	41.0
47	32.89861216852108	38.0	32.0	40.0	10.0	41.0
48	32.96845870903237	38.0	33.0	40.0	10.0	41.0
49	33.01498821306378	38.0	33.0	40.0	10.0	41.0
50	32.95839440831415	38.0	33.0	40.0	10.0	41.0
51	32.09377081059265	36.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	14.0
10	15.0
11	18.0
12	14.0
13	14.0
14	18.0
15	37.0
16	60.0
17	141.0
18	316.0
19	656.0
20	1215.0
21	1991.0
22	2915.0
23	4072.0
24	6322.0
25	10640.0
26	16648.0
27	19604.0
28	18525.0
29	16175.0
30	14505.0
31	15464.0
32	18876.0
33	25544.0
34	34001.0
35	46378.0
36	61806.0
37	91763.0
38	107178.0
39	30167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.82460855447216	13.588090149423495	16.86660123464717	30.72070006145718
2	42.46819361762629	26.232858492556343	18.43898769939185	12.859960190425523
3	23.390051275465744	24.20293710270687	39.8880929012374	12.518918720589989
4	21.548720865170292	18.410001926269732	46.413010576138106	13.62826663242187
5	24.120933048367714	21.937643896935395	38.806630036966034	15.134793017730855
6	18.063089920105668	31.325915666076554	39.92790247571524	10.683091938102534
7	55.64699731239509	5.5210559627220945	34.938863867766166	3.89308285711665
8	53.837954851906545	10.666764508938808	29.824892908575567	5.6703877305790735
9	48.2985534631578	8.02172098441556	31.017712508828737	12.662013043597906
10	28.90615397316064	20.096863849420743	37.75066731487172	13.246314862546896
11	23.489300030269952	19.832322806116366	41.61935075537292	15.059026408240767
12	19.427255799447803	18.48411744741742	45.239637127473195	16.84898962566158
13	18.31955897595832	20.99597317898715	47.60711435621314	13.077353488841394
14	15.161393885469504	25.60617873948578	43.37170584943909	15.860721525605628
15	14.106348434676525	20.65621588897348	50.47138572175493	14.76604995459507
16	14.601491483135968	22.132472321338483	44.00297195901632	19.263064236509233
17	15.248534659096121	21.903521404525815	45.26972362615691	17.578220310221155
18	14.494354195140296	22.046615727533734	46.04610205560499	17.412928021720983
19	15.666810372503875	22.801346554270356	43.698070978453295	17.83377209477247
20	16.379897082159992	23.048459442849413	47.09344242746677	13.478201047523827
21	15.764041130445152	25.17799649602363	44.35006741944065	14.707894954090573
22	14.23935277336978	21.09503847953109	45.06865775690476	19.59695099019437
23	15.10782524147167	23.865014355295866	45.11030187398527	15.916858529247197
24	16.077747915500968	21.739329841587246	44.131206486942645	18.051715755969145
25	13.403168255074805	27.049229950742532	42.59349287738834	16.95410891679432
26	13.383355194966015	22.74410882506719	46.00941120355168	17.86312477641512
27	16.083618451829498	23.082398480998727	42.94407396875774	17.88990909841404
28	12.061016886964659	23.27355782019648	47.51098432383346	17.154440969005403
29	14.906025555178456	21.63842999844064	45.07948155826049	18.37606288812042
30	13.782735119566315	24.70615213861804	43.592584778800024	17.91852796301562
31	16.115172584595346	23.510764178721143	42.458470541832156	17.915592694851355
32	16.730845082049917	26.899531274365017	39.920931213825114	16.44869242975995
33	14.918683899136848	23.945000412772085	38.28690411763087	22.849411570460195
34	16.0814170007063	26.405488951467177	40.00476981076693	17.508324237059593
35	18.165090488813878	23.931057888991827	36.486484007374855	21.417367614819437
36	17.818545391170346	25.352094589016595	39.01540098514938	17.813959034663682
37	15.94804575349251	28.278923857309273	38.135187444390425	17.637842944807787
38	16.831194562415725	26.72561663563232	35.689742155037194	20.753446646914757
39	18.440088424953448	28.320384520129522	35.804767976224326	17.434759078692704
40	18.561351690989643	25.10699969730047	36.76698557132243	19.564663040387455
41	18.701510745833293	27.97640778212972	34.528476687549876	18.79360478448711
42	19.197937974114605	25.130665296874856	37.88293783652391	17.78845889248663
43	20.784633871160075	23.604509305717354	35.468496317155726	20.14236050596685
44	16.919802970124472	25.54141938561168	36.501343802456454	21.037433841807392
45	16.66626918243609	24.52379860391308	36.66039864610756	22.149533567543273
46	19.401021840229685	26.064447481631642	36.23386749098781	18.300663187150864
47	15.760188590979554	23.67513919591998	41.8756363569653	18.68903585613517
48	17.088213981049176	25.435382823177612	37.18434401342885	20.292059182344364
49	17.140865353745678	22.792907658298095	41.36838532732827	18.697841660627965
50	17.488694631211075	22.429484768710044	38.896706078756914	21.18511452132197
51	17.070235463543053	22.200533851897376	36.57472550656308	24.154505177996498
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	623.0
1	1515.5
2	2408.0
3	22310.0
4	42212.0
5	27184.0
6	12156.0
7	11747.0
8	11338.0
9	10848.5
10	10359.0
11	9980.5
12	9602.0
13	9231.0
14	8860.0
15	8167.0
16	7474.0
17	6916.5
18	6359.0
19	6029.0
20	5699.0
21	5550.0
22	5401.0
23	5242.5
24	5084.0
25	5496.0
26	7073.0
27	8238.0
28	8796.0
29	9354.0
30	11015.0
31	12676.0
32	12686.5
33	12697.0
34	13767.0
35	14837.0
36	15487.0
37	16137.0
38	16710.5
39	17284.0
40	19211.5
41	21139.0
42	23816.0
43	26493.0
44	28122.5
45	29752.0
46	50661.5
47	71571.0
48	58467.0
49	45363.0
50	43132.0
51	40901.0
52	34085.0
53	27269.0
54	23022.0
55	18775.0
56	15506.0
57	12237.0
58	10154.5
59	8072.0
60	7051.5
61	6031.0
62	5157.0
63	4283.0
64	3626.0
65	2969.0
66	2365.0
67	1761.0
68	1509.5
69	1258.0
70	1066.5
71	875.0
72	767.5
73	660.0
74	526.5
75	340.0
76	287.0
77	224.5
78	162.0
79	118.5
80	75.0
81	51.5
82	28.0
83	24.5
84	21.0
85	15.0
86	9.0
87	6.0
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	545095.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.99542012681242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.11511494343628	32.433573754872256
2	9.310759592666173	7.6339700240219726
3	3.469886639863625	4.267483817808683
4	1.7600224916021665	2.8861144590348
5	1.0277171109338326	2.1065847367123176
6	0.7248082204654724	1.7828290505609592
7	0.5440253625579088	1.5611783808392043
8	0.45452965601247397	1.4906907366661521
9	0.31755362741402177	1.1716419931767972
>10	3.0990570633338126	24.022057346670174
>50	0.12834109219049553	3.447230388302304
>100	0.039177807089394744	3.1134547474064047
>500	0.003152237352020267	0.9012680745234549
>1k	0.004052876595454629	3.520336114929898
>5k	0.001350958865151543	4.766456455575805
>10k+	4.503196217171809E-4	4.895129918898798
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26516	4.8644731652280795	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCG	9326	1.7108944312459295	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTC	8801	1.614580944605986	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGC	7692	1.4111301699703722	No Hit
GCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	3125	0.573294563332997	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTA	2829	0.5189921022940955	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTACAGGT	2605	0.4778983479943863	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCT	2393	0.43900604481787575	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCT	2259	0.4144231739421569	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTC	1862	0.34159183261633297	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCC	1731	0.3175593245214137	No Hit
CTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT	1145	0.2100551280052101	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	1120	0.20546877149854614	Illumina Single End Adapter 2 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	966	0.17721681541749604	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATG	751	0.13777414946018585	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCTACAGGTCGT	736	0.13502233555618745	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGC	710	0.13025252478925692	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	667	0.12236399159779487	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2181271154569387	0.0
2	0.0	0.0	0.0	1.1742907199662445	0.0
3	0.0	0.0	0.0	1.4615800915436759	0.0
4	0.0	0.0	0.0	2.224933268512828	0.0
5	0.0	0.0	0.0	4.663957658756731	0.0
6	0.0	0.0	0.0	5.2444069382401235	0.0
7	0.0	0.0	0.0	6.192315101037434	0.0
8	0.0	0.0	0.0	7.269375063062402	0.0
9	0.0	0.0	0.0	7.620873425733129	0.0
10	0.0	0.0	0.0	10.237114631394528	0.0
11	0.0	0.0	0.0	11.57412928021721	0.0
12	0.0	0.0	0.0	14.14230546968877	0.0
13	0.0	0.0	0.0	14.73944908685642	0.0
14	0.0	0.0	0.0	15.004540492941597	0.0
15	0.0	0.0	0.0	15.872829506783221	0.0
16	0.0	0.0	0.0	16.62884451334171	0.0
17	0.0	0.0	0.0	17.40889202799512	0.0
18	0.0	0.0	0.0	18.269842871426082	0.0
19	0.0	0.0	0.0	19.281226208275623	0.0
20	0.0	0.0	0.0	19.871215109292876	0.0
21	0.0	0.0	0.0	20.490373237692513	0.0
22	0.0	0.0	0.0	21.163650372870784	0.0
23	0.0	0.0	0.0	21.806107192324273	0.0
24	0.0	0.0	0.0	22.30638696007118	0.0
25	0.0	0.0	0.0	22.763188068134912	0.0
26	0.0	0.0	0.0	23.199075390528257	0.0
27	0.0	0.0	0.0	23.645419605756796	0.0
28	0.0	0.0	0.0	24.08057311110907	0.0
29	0.0	0.0	0.0	24.50563663214669	0.0
30	0.0	0.0	0.0	25.01270420752346	0.0
31	0.0	0.0	0.0	25.50271053669544	0.0
32	0.0	0.0	0.0	25.99106577752502	0.0
33	0.0	0.0	0.0	26.458507232684212	0.0
34	0.0	0.0	0.0	26.924664508021536	0.0
35	0.0	0.0	0.0	27.413753565892183	0.0
36	0.0	0.0	0.0	27.88468065199644	0.0
37	0.0	0.0	0.0	28.328456507581247	0.0
38	0.0	0.0	0.0	28.792045423274843	0.0
39	0.0	0.0	0.0	29.24646162595511	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGACGGC	25	3.8880964E-5	45.000004	12
ATCGCAT	25	3.8880964E-5	45.000004	38
TCCGGCA	20	7.030018E-4	45.0	4
GGCGTAG	20	7.030018E-4	45.0	1
ACTTCGC	20	7.030018E-4	45.0	14
GTTAGCG	40	6.8030204E-9	45.0	1
GTAGGTA	20	7.030018E-4	45.0	4
TTGAGCG	35	1.2103555E-7	45.0	1
TTAACGG	20	7.030018E-4	45.0	2
CGTAAGG	40	6.8030204E-9	45.0	2
CCGTTGG	20	7.030018E-4	45.0	1
CGGGTCA	30	2.1633932E-6	44.999996	6
CGTTTTT	11505	0.0	43.35724	1
ATCCGGC	80	0.0	42.1875	6
TATGCGG	65	0.0	41.53846	2
CGGGATC	60	3.6379788E-12	41.249996	6
TGATCCG	95	0.0	40.26316	4
ACTAAGG	45	1.9250365E-8	40.000004	2
AGTACGG	45	1.9250365E-8	40.000004	2
ATGTGCG	40	3.4537698E-7	39.375	1
>>END_MODULE